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Microbiology
Michelle A. A. Wiebe, John R. R. Brannon, Bradley D. D. Steiner, Adebisi Bamidele, Alexandra C. C. Schrimpe-Rutledge, Simona G. G. Codreanu, Stacy D. D. Sherrod, John A. A. McLean, Maria Hadjifrangiskou
Summary: This study investigates a novel acid tolerance mechanism in E. coli, where l-serine is deaminated to pyruvate and ammonia, promoting the survival of the bacteria under acidic conditions.
Article
Infectious Diseases
Wedad M. Abdelraheem, W. K. M. Mahdi, Ibtehal S. Abuelela, Noha Anwar Hassuna
Summary: This study describes the occurrence and characterization of fosfomycin-resistant uropathogenic E. coli (UPEC) isolated from children with urinary tract infections. The results showed a high prevalence of fosfomycin resistance among UPEC isolates, as well as high resistance to other antibiotics. Close monitoring of these strains is necessary to prevent the widespread dissemination of antibiotic resistance genes.
BMC INFECTIOUS DISEASES
(2023)
Article
Microbiology
Arwen E. Frick-Cheng, Anna Sintsova, Sara N. Smith, Ali Pirani, Evan S. Snitkin, Harry L. T. Mobley
Summary: More than half of women will experience a urinary tract infection (UTI), with uropathogenic Escherichia coli (UPEC) causing the majority of cases. Iron acquisition systems play a crucial role in UPEC pathogenesis, and this study reveals the importance of the ferric citrate uptake system as an alternative iron source when other siderophores are limited. Understanding these iron acquisition mechanisms is important for developing new strategies to combat UTI.
Article
Pharmacology & Pharmacy
Leila Mousavifar, Meysam Sarshar, Clarisse Bridot, Daniela Scribano, Cecilia Ambrosi, Anna Teresa Palamara, Gerard Vergoten, Benoit Roubinet, Ludovic Landemarre, Julie Bouckaert, Rene Roy
Summary: A small library of FimH antagonists based on previously described C-linked allyl alpha-D-mannopyranoside was synthesized. Two new members with sub nanomolar affinity were identified and shown to have improved binding compared to previously published analogs. The crystal structure of FimH co-crystallized with one of the antagonists revealed its binding mode, and the antagonists were effective in decreasing bacterial adhesion to human bladder epithelial cells.
Article
Microbiology
Mahta Yousefipour, Seyedeh Elham Rezatofighi, Mohammad Roayaei Ardakani
Summary: The main aetiological agent of urinary tract infection is Escherichia coli, with some "intermediary strains" carrying virulence markers of intestinal pathogenic E. coli. This suggests that intestinal pathogenic E. coli have a greater tendency to form hybrid strains and cause UTI.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Bill Soderstrom, Matthew J. Pittorino, Daniel O. Daley, Iain G. Duggin
Summary: During infection, some uropathogenic Escherichia coli exhibit filamentation, a stress response that promotes bacterial dispersal and survival. This process involves dynamic repositioning of FtsZ division rings and the distribution of DamX protein throughout the cell envelope.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Camille Chagneau, Clemence Massip, Nadege Bossuet-Greif, Christophe Fremez, Jean-Paul Motta, Ayaka Shima, Celine Besson, Pauline Le Faouder, Nicolas Cenac, Marie-Paule Roth, Helene Coppin, Maxime Fontanie, Patricia Martin, Jean-Philippe Nougayrede, Eric Oswald
Summary: This study found the presence of the genotoxin Colibactin in the urine of patients with urinary tract infections, and demonstrated its genotoxicity in bladder cells. This discovery raises the hypothesis of a potential link between urinary tract infections and bladder cancer.
Article
Microbiology
Noble Selasi Gati, Imke Johanna Temme, Barbara Middendorf-Bauchart, Alexander Kehl, Ulrich Dobrindt, Alexander Mellmann
Summary: Hybrid Shiga toxin-producing Escherichia coli strains are positioned between STEC and UPEC, causing both diarrhea and urinary tract infections, with potential to adapt to both intestinal and extraintestinal environments.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Immunology
Naseem Maalouf, Chamutal Gur, Vladimir Yutkin, Viviana Scaiewicz, Ofer Mandelboim, Gilad Bachrach
Summary: Bladder cancer cells express high levels of mannose, and mannose-targeted therapy holds potential for improving bladder cancer treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Dorota Wojnicz, Dorota Tichaczek-Goska, Michal Glensk, Andrzej B. Hendrich
Summary: The study found that European goldenrod extract can limit the survival of planktonic forms of bacteria and reduce biofilm formation, but when combined with antibiotics, it weakens their antibacterial activity and shortens the duration of post-antibiotic effects.
Article
Biochemistry & Molecular Biology
Yeseul Kim, Sanghun Kim, Kiu-Hyung Cho, Jin-Hyung Lee, Jintae Lee
Summary: Bacterial biofilm formation can be effectively inhibited by cinnamaldehyde and its derivatives, especially 4-nitrocinnamaldehyde. These compounds exhibit strong antibiofilm activities against UPEC and S. aureus, making them promising candidates for the treatment of biofilm-associated diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Kate R. Fortney, Sara N. Smith, Julia J. van Rensburg, Julie A. Brothwell, Jessi J. Gardner, Barry P. Katz, Nagib Ahsan, Adam S. Duerfeldt, Harry L. T. Mobley, Stanley M. Spinola
Summary: This study suggests that chemical activation of the CpxRA system may be a viable strategy for treating infections caused by uropathogenic Escherichia coli (UPEC). The most potent compound significantly reduced bacterial recovery in the urine and downregulated multiple proteins involved in UPEC virulence.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Si Zhang, Jingting Wang, Yu Fan, Wang Meng, Chengqian Qian, Peng Liu, Yi Wei, Chao Yuan, Yuhui Du, Zhiqiu Yin
Summary: Urinary tract infections (UTIs), characterized by recurrence and antibiotic resistance, remain a common health and economic issue. This study focuses on Uropathogenic Escherichia coli (UPEC) and its ability to evade the host immune response through the formation of intracellular bacterial communities (IBCs) in bladder epithelial cells (BECs). The researchers discovered that the small molecule cyclic dimeric guanosine monophosphate (c-di-GMP) and the phosphodiesterase YciR play important roles in this process. They found that YciR enhances the virulence of UPEC by inhibiting the phosphorylation of NF-kappa B.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
George L. Katumba, Hung Tran, Jeffrey P. Henderson
Summary: This study reveals that extracellular copper ions can stimulate the production of multifunctional iron siderophore Ybt in extraintestinal pathogenic Escherichia coli, suggesting the important role of dual regulation in infection pathogenesis.
Article
Microbiology
George L. Katumba, Hung Tran, Jeffrey P. Henderson
Summary: Siderophores are iron chelators utilized by microbes to acquire iron, but the study found extracellular copper ions can rapidly stimulate Ybt production in extraintestinal pathogenic Escherichia coli. This stimulation pathway involves formation of a Cu(II)-Ybt complex, internalization through a TonB-dependent outer membrane transporter, and transcriptional regulation by the specialized transcription factor YbtA. The dual regulation by iron and copper indicates a multifunctional metallophore role for Ybt in stress responses during infection pathogenesis.
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Craig Winstanley, Michael A. Brockhurst
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Robert William O'Hara, Peter J. Jenks, Matthew Emery, Mathew Upton
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(2020)
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Infectious Diseases
Sean D. W. Comber, Mathew Upton, Shaun Lewin, Neil Powell, Thomas H. Hutchinson
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Katharine Hammond, Helen Lewis, Samantha Halliwell, Florie Desriac, Brunello Nardone, Jascindra Ravi, Bart W. Hoogenboom, Mathew Upton, Jeremy P. Derrick, Maxim G. Ryadnov
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Microbiology
James Butler, Sean D. Kelly, Katie J. Muddiman, Alexandros Besinis, Mathew Upton
Summary: This study reports the characterization of a strain of C. pauculus isolated from a hospital sink trap and assesses its potential risk to human health. The findings show that the strain is multidrug-resistant and capable of biofilm formation.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
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Microbiology
Matthew J. Koch, Poppy J. Hesketh-Best, Gary Smerdon, Philip J. Warburton, Kerry Howell, Mathew Upton
Summary: Through culture-based microbial analysis of deep-sea sponges, this study discovered novel antimicrobial candidates and demonstrated antimicrobial activity, showcasing the potential for screening novel antimicrobial compounds.
Review
Infectious Diseases
Neil Powell, Jennie Stephens, Declan Kohl, Rhys Owens, Shadia Ahmed, Crispin Musicha, Mathew Upton, Bridie Kent, Sarah Tonkin-Crine, Jonathan Sandoe
Summary: This systematic review examined the effectiveness and safety of nonallergist health care worker delivery of penicillin allergy delabeling. The results showed that delabeling by nonallergists is effective and safe, with different testing methods leading to varying proportions of patients being delabeled.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
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Microbiology
Pisut Pongchaikul, Pitak Santanirand, Svetlana Antonyuk, Craig Winstanley, Alistair C. Darby
FEMS MICROBIOLOGY LETTERS
(2020)
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Respiratory System
Freddy J. Frost, Dilip S. Nazareth, Susan C. Charman, Craig Winstanley, Martin J. Walshaw
ANNALS OF THE AMERICAN THORACIC SOCIETY
(2019)