Article
Food Science & Technology
Jing Dong, Tianhui Yan, Qiuhong Yang, Yi Song, Bo Cheng, Shun Zhou, Yongtao Liu, Xiaohui Ai
Summary: Aeromonas hydrophila is a common pathogen in freshwater aquaculture that can also cause health problems in humans. Antibiotic-resistant strains of A. hydrophila are a concern as they limit antibiotic usage and can lead to treatment failure. Additionally, antibiotic residues in aquatic products pose a risk to quality and safety. This study explores alternative strategies to combat infections caused by antibiotic-resistant bacteria, focusing on palmatine, a compound that inhibits the expression of a key virulence factor in A. hydrophila. The findings suggest that palmatine could be a promising treatment for A. hydrophila-associated infections in aquaculture.
Article
Veterinary Sciences
Jing Dong, Tianhui Yan, Qiuhong Yang, Shun Zhou, Yi Song, Yongtao Liu, Liang Ma, Ning Xu, Yibin Yang, Xiaohui Ai
Summary: The fast-growing demand for aquatic products has led to the rapid development of aquaculture. However, antibiotic resistance caused treatment failure in the control of diseases caused by bacterial pathogens. In this study, researchers identified aerolysin as a target for developing drugs and found that polydatin could decrease the pathogenesis of antibiotic-resistant A. hydrophila infections.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Kyoo-Tae Kim, Seung-Hun Lee, Kyoung-Ki Lee, Jee Eun Han, Dongmi Kwak
Summary: The research suggests that stress can increase the virulence of Aeromonas hydrophila and lead to animal mortality, while serial passaging in mice can dramatically enhance the virulence of A. hydrophila.
Article
Immunology
Jing Dong, Lushan Zhang, Yongtao Liu, Ning Xu, Shun Zhou, Yibin Yang, Qiuhong Yang, Xiaohui Ai
Summary: The study found that luteolin can reduce aerolysin-induced hemolysis by inhibiting aerolysin activity, and hinder the formation of functional heptamer. In addition, luteolin can protect cells from aerolysin-induced cell death and increase the survival rate in A. hydrophila-infected channel catfish.
Article
Pharmacology & Pharmacy
Helena Hader, Nina A. Hering, Joerg-Dieter Schulzke, Roland Buecker, Rita Rosenthal
Summary: The study demonstrates the antagonizing effects of myrrh against IL-13-induced barrier impairment in a human intestinal cell model. This suggests the potential use of myrrh as a treatment option for inflammatory bowel disease.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Immunology
Zhiyuan Lu, Lin Feng, Wei-Dan Jiang, Pei Wu, Yang Liu, Jun Jiang, Sheng-Yao Kuang, Ling Tang, Shu-Wei Li, Xiang-An Liu, Cheng-Bo Zhong, Xiao-Qiu Zhou
Summary: The study found that dietary Mannan oligosaccharides (MOS) supplementation can reduce skin lesion morbidity in grass carp challenged by pathogenic bacteria, maintain skin barrier function by improving antioxidant capacity, inhibiting cell apoptosis, and strengthening cell connections. Additionally, MOS can regulate immune barrier function by modulating the expression of immune factors and related signaling pathways.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Chunnuan Zhang, Xiaoyu Yuan, Ruiyi Xu, Qian Qi, Yang Wang
Summary: This study investigated the effects of Aeromonas hydrophila infection on intestinal histopathology, innate immune response, and antioxidant capacity in blunt snout bream. The results showed that infection induced changes in intestinal morphology, reduced innate immune enzyme activities and antioxidant enzyme activities, and increased expression of inflammatory cytokines. These findings highlight the impact of Aeromonas hydrophila infection on intestinal injury, immune response, and antioxidant capacity in blunt snout bream.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Chen Feng, Xiaofeng Liu, Niewen Hu, Yiyang Tang, Mengzhe Feng, Zejun Zhou
Summary: The secretory serine protease Ssp1 in Aeromonas hydrophila plays a crucial role in its pathogenicity by increasing intestinal permeability and disrupting tight junctions. The mutant strain delta Ssp1 exhibited reduced virulence, but showed no differences in biofilm formation, swimming motility, and resistance to environmental stress.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Franz-Leonard Klaus, Cornelia Kirsch, Joerg P. Mueller, Otmar Huber, Juliane Reiche
Summary: Phosphoinositide 3-kinases (PI3Ks) are enzymes that phosphorylate phospholipids in the cell membrane, promoting the PI3K/AKT signaling cascade. They play a significant role in various cellular functions, including TNF alpha-induced tight junction (TJ) impairment in inflammatory bowel diseases. This study focuses on the functional role of PI3K gamma in HT-29/B6 cells under inflammatory conditions. It is found that PI3K gamma deficiency protects the cells against TNF alpha-induced barrier dysfunction, independent of any PI3K activity.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Food Science & Technology
Lucas Heils, Martina Schneemann, Ralf Gerhard, Joerg-Dieter Schulzke, Roland Buecker
Summary: The study investigated the impact of Clostridioides difficile binary toxin (CDT) on the intestinal epithelial barrier and found that sublethal concentrations of CDT induced barrier dysfunction with increased permeability and redistribution of tight junction proteins. This suggests that CDT may contribute to diarrhea by compromising the intestinal barrier function.
Article
Biotechnology & Applied Microbiology
Xiaofang Lai, Jing Chen, Shenyuan Liang, Hao Chen, Sen Liu, Huan Gao
Summary: This study found that Psychrobacter sp. B6 can significantly improve the growth and immunity of Exopalaemon carinicauda. It increases the activity of digestive enzymes and antioxidant enzymes, and enhances the resistance against pathogenic bacteria.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Cell Biology
Yihua Luo, Xueyan Yu, Peizhuang Zhao, Jun Huang, Xue Huang
Summary: Resveratrol (RSV) has been found to regulate the expression of intestinal epithelial tight junction proteins by attenuating the Notch1 pathway, thereby exerting anti-inflammatory effects.
Article
Biochemistry & Molecular Biology
Restituto Tocmo, Bryan Le, Amber Heun, Jan Peter van Pijkeren, Kirk Parkin, Jeremy James Johnson
Summary: Xanthones from mangosteen exhibit anti-inflammatory and anti-oxidative effects. The study isolated seven prenylated xanthones from mangosteen fruit pericarp extracts, with Garcinone D (GarD) showing dual protection against intestinal epithelial barrier dysfunction through AhR and Nrf2 pathways.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Microbiology
Weiming Zhong, Kangyong Chen, Linlin Yang, Tao Tang, Sifan Jiang, Jiajing Guo, Zhipeng Gao
Summary: This study evaluated the antibacterial activity of essential oil from satsuma mandarin against Aeromonas hydrophila, demonstrating strong potential for controlling and preventing infections in aquaculture.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Puqiao Lian, Saskia Braber, Soheil Varasteh, Harry J. Wichers, Gert Folkerts
Summary: Hypoxia and hyperthermia can disrupt intestinal epithelial integrity and barrier function, triggering heat shock and oxidative stress responses. A Caco-2/HT-29 cell model was developed to study these effects, highlighting potential interventions for hyperthermia and/or hypoxia-induced intestinal injury.
SCIENTIFIC REPORTS
(2021)
Article
Urology & Nephrology
Tilman Breiderhoff, Nina Himmerkus, Luca Meoli, Anja Fromm, Sebastian Sewerin, Natalia Kriuchkova, Oliver Nagel, Yury Ladilov, Susanne M. Krug, Catarina Quintanova, Meike Stumpp, Dieter Garbe-Schoenberg, Ulrike Westernstroeer, Cosima Merkel, Merle Annette Brinkhus, Janine Altmuller, Michal R. Schweiger, Dominik Muller, Kerim Mutig, Markus Morawski, Jan Halbritter, Susanne Milatz, Markus Bleich, Dorothee Guenzel
Summary: Recent study indicates that claudin-10a is the major paracellular anion channel in the proximal tubule, and its deficiency leads to excessive reabsorption of calcium and magnesium. Various analyses, including electrophysiological studies, suggest compensatory transcellular transport in proximal and distal tubule segments, as well as metabolic adaptation in the proximal tubule, to counterbalance the loss of paracellular anion permeability.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Multidisciplinary Sciences
Caroline Hempel, Rita Rosenthal, Anja Fromm, Susanne M. Krug, Michael Fromm, Dorothee Gunzel, Joerg Piontek
Summary: This study analyzed the structural and functional differences between claudin-15 and claudin-10b cation channels and identified several mutants that affect ion permeability and pore diameter. Furthermore, the study revealed that the charge at the W63/K64 position has an impact on the assembly and properties of claudin-10b channels but not claudin-15 channels.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Immunology
Yiyu Wang, Joerg Scheffel, Carolina Ayala Vera, Wei Liu, Dorothee Guenzel, Dorothea Terhorst-Molawi, Marcus Maurer, Sabine Altrichter
Summary: This study aimed to assess the rate and extent of impaired sweating in patients with cholinergic urticaria (CholU) and its association with disease duration and severity. The results showed that reduced sweating is common in CholU patients, especially in those with long-standing and severe disease. The reduced expression of CHRM3 and ACh-E may be responsible for the impaired sweating in these patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Laura-Sophie Beier, Ayk Waldow, Saeed Khomeijani Farahani, Roman Mannweiler, Sabine Vidal-Y-Sy, Johanna M. Brandner, Jorg Piontek, Dorothee Gunzel
Summary: The formation of tight junction (TJ) is crucial for epidermal barrier function. Manipulation of TJ barriers in reconstructed human epidermis (RHE) was achieved through knockdown of claudin-1 and -4 or by using claudin-binding fusion proteins. The study demonstrates the critical role of claudin-1 in regulating paracellular permeability in the viable epidermis.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Multidisciplinary Sciences
Catarina Quintanova, Nina Himmerkus, Samuel L. Svendsen, Otto von Schwerdtner, Cosima Merkel, Lennart Pinckert, Kerim Mutig, Tilman Breiderhoff, Dominik Mueller, Dorothee Guenzel, Markus Bleich
Summary: Claudin-10b is an important component of the tight junction and allows paracellular sodium transport in the thick ascending limb of Henle's loop. It colocalizes with the basolateral Na+-K+ ATPase and the Cl- channel subunit barttin, and its absence increases the accessibility of the basolateral extracellular space to certain substances. Electron microscopy reveals a widening of basolateral membrane infoldings in the absence of Claudin-10b. These findings suggest that Claudin-10b may shape neighboring membrane invaginations through trans interaction, thereby stabilizing and facilitating high-flux salt transport.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Physiology
Natalia Kriuchkova, Tilman Breiderhoff, Dominik Mueller, Duygu Elif Yilmaz, Hasan Demirci, Hoora Drewell, Dorothee Guenzel, Nina Himmerkus, Markus Bleich, Pontus B. Persson, Kerim Mutig
Summary: Perturbed calcium homeostasis in familial hypomagnesaemia with hypercalciuria and nephrocalcinosis (FHHNC) limits life expectancy. FHHNC is caused by loss-of-function mutations in CLDN16 or CLDN19 genes, leading to impaired paracellular reabsorption of divalent cations in the cortical thick ascending limb (cTAL). Furosemide, a loop diuretic, can compensate for this impairment by activating Ca2+ transport proteins in the distal nephron and collecting duct, resulting in reduced hypercalciuria.
Article
Physiology
Dorothee Guenzel
Summary: The concept of solvent drag involves the frictional coupling between water and solutes in the same pore for transport. It has been applied to transport processes across cell membranes and along the paracellular pathway. Water-driven solute transport has been proposed as the major mechanism for absorption in the small intestine and reabsorption in the renal proximal tubule. However, the discovery of aquaporins and the claudin protein family has led to a reassessment of the solvent drag concept for transepithelial water and solute transport.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2023)
Article
Cell Biology
Karem Awad, Christian Barmeyer, Christian Bojarski, Oliver Nagel, In-Fah M. Lee, Michal R. Schweiger, Joerg-Dieter Schulzke, Roland Buecker
Summary: This study investigated the pathophysiological mechanisms of diarrhea, constipation, and antigen uptake in mixed-type irritable bowel syndrome (IBS-M). The findings showed that in IBS-M, chloride secretion was reduced and antigen permeability was increased, which contributed to a low-grade inflammation in the colon mucosa.
Article
Cell Biology
Thomas Sell, Christian Klotz, Matthias M. Fischer, Rosario Astaburuaga-Garcia, Susanne Krug, Jarno Drost, Hans Clevers, Christine Sers, Markus Morkel, Nils Bluethgen
Summary: Colorectal cancer progression is closely related to deregulation of intestinal differentiation trajectory. The sequential mutations of APC, KRAS, TP53, and SMAD4 enable oncogenic signaling and establish cancer hallmarks. Mass cytometry of isogenic human colon and patient-derived cancer organoids reveals a differentiation axis from normal to cancer states, shaped by the driver mutations. Cells along this axis can be influenced by subsequent mutations to promote or restrict stem cell properties. Nodes of the cancer cell signaling network remain coupled to the differentiation state. Single-cell RNA sequencing links protein signaling network to transcriptomic states with biological and clinical importance.
JOURNAL OF CELL BIOLOGY
(2023)
Review
Urology & Nephrology
Luca Meoli, Dorothee Guenzel
Summary: Sequential expression of claudins in the nephron mirrors the sequential expression of ion channels and transporters, contributing to the maintenance of electrolyte and water homeostasis. The regulation and dysregulation of claudins have been extensively studied in the gastrointestinal tract, but their role in the kidney is only just emerging. Investigating the role of claudins in kidney diseases may provide insights into potential prognostic markers or druggable targets.
NATURE REVIEWS NEPHROLOGY
(2023)
Article
Multidisciplinary Sciences
Matthias Wuttke, Eva Koenig, Maria-Alexandra Katsara, Holger Kirsten, Saeed Khomeijani Farahani, Alexander Teumer, Yong Li, Martin Lang, Burulca Goecmen, Cristian Pattaro, Dorothee Guenzel, Anna Koettgen, Christian Fuchsberger
Summary: A genotype imputation approach was applied to whole exome sequencing data from the UK Biobank, resulting in the discovery of 158 rare variants and 105 genes significantly associated with kidney function traits. This approach boosts statistical power and provides a comprehensive resource for directing experimental and clinical studies of kidney disease.
NATURE COMMUNICATIONS
(2023)
Article
Food Science & Technology
Martina Schneemann, Lucas Heils, Verena Moos, Franziska Weiss, Susanne M. Krug, January Weiner, Dieter Beule, Ralf Gerhard, Joerg-Dieter Schulzke, Roland Buecker
Summary: This study investigated the effects of Clostridioides difficile toxins TcdA and TcdB on epithelial barrier function using an organoid model. The toxins caused a decrease in transepithelial electrical resistance and an increase in paracellular permeability in the organoid monolayers. Redistribution of tight junction proteins and actin cytoskeleton organization were also observed. These findings provide insights into the mechanisms underlying diarrhea and inflammation in patients.
Article
Infectious Diseases
Cristiana Mateus, Claudio J. Maia, Fernanda Domingues, Roland Buecker, Monica Oleastro, Susana Ferreira
Summary: Aliarcobacter butzleri can survive in physiological concentrations of bile salts and exposure to bile salts can change its virulence mechanisms.
Article
Biochemistry & Molecular Biology
Leonie Wittner, Lukas Wagener, Jakob J. Wiese, Iris Stolzer, Susanne M. Krug, Elisabeth Naschberger, Rene Jackstadt, Rudi Beyaert, Raja Atreya, Anja A. Kuehl, Gregor Sturm, Miguel Gonzalez-Acera, Jay V. Patankar, Christoph Becker, Britta Siegmund, Zlatko Trajanoski, Beate Winner, Markus F. Neurath, Michael Schumann, Claudia Guenther
Summary: The paracaspase MALT1 plays a crucial role in mucosal inflammation. It is highly expressed in colonic epithelial cells of UC patients and experimental colitis. Mechanistically, MALT1 protease function inhibits ferroptosis and contributes to NF-kappa B signaling and STAT3 signaling, which are involved in inflammation and tissue healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
David Holthaus, Martin R. Kraft, Susanne M. Krug, Silver Wolf, Antonia Mueller, Estefania Delgado Betancourt, Madeleine Schorr, Gudrun Holland, Felix Knauf, Joerg-Dieter Schulzke, Toni Aebischer, Christian Klotz
Summary: This study used a human organoid model to recapitulate the pathogenic events leading to gastrointestinal illness caused by Giardia duodenalis, revealing a previously unknown sequence of events that resulted in intestinal barrier dysfunction.
