Article
Immunology
Thijs ten Doesschate, Sander Kuiper, Cees van Nieuwkoop, Robert-Jan Hassing, Tom Ketels, Suzan P. van Mens, Wouter van den Bijllaardt, Akke K. van der Bij, Suzanne E. Geerlings, Ad Koster, Evert L. Koldewijn, Judith Branger, Andy I. M. Hoepelman, Cornelis H. van Werkhoven, Marc J. M. Bonten
Summary: Fosfomycin is noninferior to ciprofloxacin as oral step-down treatment for febrile urinary tract infections (fUTIs) caused by E. coli in women, but its use is associated with more gastrointestinal events.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Medicine, General & Internal
Katarzyna Werbel, Dorota Jankowska, Anna Wasilewska, Katarzyna Taranta-Janusz
Summary: The study analyzed the increasing resistance of bacteria in children with UTI over the years, identified parameters related to antibiotic sensitivity using the CART method, and provided guidance for antibiotic therapy to inhibit increasing antibiotic resistance.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Infectious Diseases
Guadalupe Miranda-Novales, Karen Flores-Moreno, Yolanda Lopez-Vidal, Samuel Ponce de Leon-Rosales
Summary: This study evaluated the antimicrobial susceptibility patterns of Escherichia coli and Klebsiella pneumoniae, and found that multidrug-resistant bacteria represented 61% of all isolates.
Review
Nutrition & Dietetics
Reeta Ala-Jaakkola, Arja Laitila, Arthur C. Ouwehand, Liisa Lehtoranta
Summary: Urinary tract infections (UTIs) are a common bacterial disease worldwide, and antibiotic resistance is a major concern. D-mannose, a dietary supplement, shows promise as an alternative treatment for UTIs, reducing the risk and recurrence.
Article
Immunology
Mohamed Kettani Halabi, Fatima Azzahra Lahlou, Idrissa Diawara, Younes El Adouzi, Rabiaa Marnaoui, Rachid Benmessaoud, Imane Smyej
Summary: ESBL-EC infections pose challenges due to limited treatment options, increased hospital costs, reduced microbiological and clinical responses, and a threat to patient's life. Majority of ESBL-EC isolates were from female adult patients over 50, resistant to third-generation cephalosporins and quinolones, but sensitive to carbapenems and fosfomycin.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Younjung Kim, Maura Carrai, Marcus H. Y. Leung, Jaime Chin, Jun Li, Patrick K. H. Lee, Julia A. Beatty, Dirk U. Pfeiffer, Vanessa R. Barrs
Summary: The study found that the feline bladder microbiome is typically sparse, with various phyla, families, and genera identified. Different urotypes were associated with disease status, with the Escherichia-Shigella-predominant urotype being common in CKD cases. The microbial composition of CKD cases was more similar to that of E. coli PUC cases than to controls, indicating potential implications for the development of clinical infections.
Article
Microbiology
Lotte Jakobsen, Carina Vingsbro Lundberg, Niels Frimodt-Moller
Summary: Ciprofloxacin is highly effective in clearing urine and kidney tissue for fully susceptible E. coli, but not for low-level resistant strains. PKPD correlations show a higher correlation between AUC/MIC and effect in urine and kidneys, while %T->MIC shows a lower correlation.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Biochemistry & Molecular Biology
Maria Maisto, Fortuna Iannuzzo, Ettore Novellino, Elisabetta Schiano, Vincenzo Piccolo, Gian Carlo Tenore
Summary: Urinary tract infections (UTIs) are a common bacterial infection, especially in women. The main etiological agents are E. coli, Pseudomonas aeruginosa, and Proteus mirabilis. Due to the increase in antimicrobial resistance, there is a need for natural alternatives for UTI treatment. This review summarizes the potential therapeutic effects of natural polyphenol-based nutraceuticals and foods through in vitro and in vivo studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Lei Huang, Chenwei Huang, Yan Yan, Liying Sun, Haixia Li
Summary: This study compared the etiological profiles and antibiotic resistance patterns of urinary tract infections (UTIs) sorted by different age categories. The study found that the pathogens and antibiotic resistance varied among different age groups, especially in pediatric and geriatric patients. Therefore, different antibiotic therapies should be considered for different age categories when initiating empirical antimicrobial therapies.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Amanda Brumwell, Granger Sutton, Paul M. M. Lantos, Kate Hoffman, Felicia Ruffin, Lauren Brinkac, Thomas H. H. Clarke, Mark D. D. Adams, Vance G. G. Fowler Jr, Derrick E. E. Fouts, Joshua T. T. Thaden
Summary: This study aims to further understand the risk factors, clinical outcomes, and bacterial genetics associated with ST131 bloodstream infections. A cohort study was conducted in which 88 out of 227 patients with E. coli bloodstream infection were infected with ST131. There was no significant difference in in-hospital mortality between patients with ST131 and non-ST131 infections overall, but ST131 was associated with higher mortality in patients with urinary tract infections.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Maria L. Jensen, Volkert Siersma, Lillian M. Soes, Dagny Nicolaisdottir, Lars Bjerrum, Barbara J. Holzknecht
Summary: We investigated the impact of prior antibiotic use on multi-resistant Escherichia coli urinary tract infections (UTIs) among elderly patients in general practice. The study found that exposure to antibiotics within 90 days prior to the UTI episode significantly increased the odds of resistance to all four antibiotics. Mecillinam showed the lowest increase in resistance, suggesting it as a favorable antibiotic choice.
Article
Immunology
Jennifer H. Ku, Katia J. Bruxvoort, S. Bianca Salas, Cara D. Varley, Joan A. Casey, Eva Raphael, Sarah C. Robinson, Keeve E. Nachman, Bruno J. Lewin, Richard Contreras, Rong X. Wei, Magdalena E. Pomichowski, Harpreet S. Takhar, Sara Y. Tartof
Summary: A study showed that the prevalence of multidrug-resistant Escherichia coli causing outpatient urinary tract infection declined overall between 2016 and 2021. The choice of care setting (in-person vs virtual) did not significantly affect the treatment of infections. Virtual healthcare can expand access to UTI care.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Microbiology
Cheng-Yen Kao, Yen-Zheng Zhang, Deng-Chi Yang, Pek Kee Chen, Ching-Hao Teng, Wei-Hung Lin, Ming-Cheng Wang
Summary: This study investigated the characteristics of host and bacteria in recurrent urinary tract infections (RUTI) caused by genetically identical or different Escherichia coli strains. The results showed that E. coli strains in RUTI were more virulent and important for the development of RUTI in patients who were younger, without anatomical/functional defects or immune dysfunction. Prior antibiotic therapy, especially fluoroquinolones, could induce subsequent antimicrobial resistance in genetically highly-related E. coli RUTI.
Review
Infectious Diseases
Iain J. Abbott, Trisha N. Peel, Kelly A. Cairns, Andrew J. Stewardson
Summary: This article highlights the key clinical decisions involved in the diagnosis and treatment of urinary tract infections (UTIs) in adult women, emphasizing the importance of clinical effectiveness and antimicrobial stewardship in the post-antimicrobial era. The article discusses the diagnosis and treatment of UTIs, as well as emerging areas of research in this field.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Emergency Medicine
Jessica L. Wesolek, Janet Y. Wu, Courtney M. Smalley, Lu Wang, Matthew J. Campbell
Summary: Identifying risk factors associated with TMP-SMX resistance in E. coli can guide empiric antibiotic prescribing for urinary tract infections. This study identified three predictors of TMP-SMX resistance: recurrent UTIs, genitourinary abnormalities, and recent use of TMP-SMX. The resistance rate of TMP-SMX was higher in the emergency department population compared to the institutional antibiogram.
AMERICAN JOURNAL OF EMERGENCY MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Sebastien Crepin, Elizabeth N. Ottosen, Courtney E. Chandler, Anna Sintsova, Robert K. Ernst, Harry L. T. Mobley
MOLECULAR MICROBIOLOGY
(2020)
Article
Genetics & Heredity
Amol C. Shetty, John Mattick, Matthew Chung, Carrie McCracken, Anup Mahurkar, Scott G. Filler, Claire M. Fraser, David A. Rasko, Vincent M. Bruno, Julie C. Dunning Hotopp
MICROBIAL GENOMICS
(2020)
Article
Infectious Diseases
Rodrigo T. Hernandes, Tracy H. Hazen, Luis F. dos Santos, Taylor K. S. Richter, Jane M. Michalski, David A. Rasko
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Microbiology
Wanghsu Jiang, Wimal Ubhayasekera, Michael C. Breed, Allison N. Norsworthy, Nina Serr, Harry L. T. Mobley, Melanie M. Pearson, Stefan D. Knight
Article
Microbiology
Laura A. Mike, Andrew J. Stark, Valerie S. Forsyth, Jay Vornhagen, Sara N. Smith, Michael A. Bachman, Harry L. T. Mobley
Summary: The study identifies a close relationship between capsule biosynthesis and hypermucoviscosity in Klebsiella pneumoniae, with central metabolism playing a key regulatory role. Certain metabolic genes specifically impact only one of these properties, with genes related to the TCA cycle playing a significant role.
Article
Microbiology
Mark T. Anderson, Aric N. Brown, Ali Pirani, Sara N. Smith, Amanda L. Photenhauer, Yuang Sun, Evan S. Snitkin, Michael A. Bachman, Harry L. T. Mobley
Summary: Bloodstream infections, caused by Gram-negative species, are a significant public health burden due to high mortality rates and treatment costs. A study characterizing infection kinetics and bacterial replication rates of six common pathogens in a murine model revealed different colonization abilities in major organs. While some species replicated rapidly in the liver or kidney, others were steadily cleared from targeted organs during infection, highlighting the complex dynamics of bacterial replication and immune-mediated clearance. Insights from this study may aid in developing strategies to combat bloodstream infections in the future.
Article
Immunology
Allyson E. Shea, Jolie A. Stocki, Stephanie D. Himpsl, Sara N. Smith, Harry L. T. Mobley
Summary: Uropathogenic Escherichia coli (UPEC) causes urinary tract infections (UTI) in nearly half of women worldwide, and the role of the ila locus in UPEC uropathogenesis remains unclear. This study found that the ila locus is related to bladder cell adherence and invasion, and its loss may affect the fitness of UPEC during UTI.
INFECTION AND IMMUNITY
(2022)
Review
Microbiology
Aric N. Brown, Mark T. Anderson, Michael A. Bachman, Harry L. T. Mobley
Summary: ArcAB, a member of the bacterial transcriptional regulator two-component system family, acts as a sensor of oxygen consumption. This review provides an overview of the structure and function of ArcAB proteins and discusses the literature on its role as an oxygen consumption sensor. The regulation of ArcAB activity is primarily mediated by the bacterial quinone pool, but the exact mechanism is still not fully understood. The review also explores the involvement of ArcAB in regulating central metabolic pathways and its potential interactions with other regulatory systems.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2022)
Review
Microbiology
Pradip Kumar Singh, Janay Little, Michael S. Donnenberg
Summary: This article introduces the importance of type IV pili (T4P) and their impact on microbiology, and reviews the important discoveries and research milestones in this field. It also points out some important discoveries that are often overlooked in current reports.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2022)
Article
Microbiology
Arwen E. Frick-Cheng, Anna Sintsova, Sara N. Smith, Ali Pirani, Evan S. Snitkin, Harry L. T. Mobley
Summary: More than half of women will experience a urinary tract infection (UTI), with uropathogenic Escherichia coli (UPEC) causing the majority of cases. Iron acquisition systems play a crucial role in UPEC pathogenesis, and this study reveals the importance of the ferric citrate uptake system as an alternative iron source when other siderophores are limited. Understanding these iron acquisition mechanisms is important for developing new strategies to combat UTI.
Article
Microbiology
Ashok R. Nayak, Pradip K. Singh, Jinlei Zhao, Montserrat Samso, Michael S. Donnenberg
Summary: Type 4 pili are important surface appendages on bacteria and archaea that can extend and retract with great force. This study presents the detailed structure of an enzyme that converts chemical energy to mechanical energy in a human pathogen, revealing a mechanism of action not previously seen in enzymes that power type 4 pilus extension.
Article
Immunology
Sophia Mason, Jay Vornhagen, Sara N. Smith, Laura A. Mike, Harry L. T. Mobley, Michael A. Bachman
Summary: Healthcare-acquired infections, particularly caused by Klebsiella pneumoniae, are a major concern for hospitalized patients and long-term-care facilities. Previous studies have linked the ter operon to infection in colonized patients. This study further explores the role of the ter operon in enhancing fitness during gut colonization and bladder infection.
INFECTION AND IMMUNITY
(2023)
Article
Microbiology
A. E. Shea, A. E. Frick-Cheng, S. N. Smith, H. L. T. Mobley
Summary: This study demonstrates that phenotypic characteristics can be used to predict the severity of urinary tract infection caused by uropathogenic Escherichia coli (UPEC) strains. These characteristics can help differentiate UPEC from other E. coli strains and reduce the overuse of antibiotics.
Article
Microbiology
Melanie M. Pearson, Sapna Pahil, Valerie S. Forsyth, Allyson E. Shea, Harry L. T. Mobley
Summary: Ordered transposon libraries are a valuable resource for studying bacterial species, especially those with difficult methods for targeted genetic mutations. In this study, we constructed an ordered transposon library for the bacterial urinary tract pathogen Proteus mirabilis strain HI4320. The library enables large-scale screens and facilitates the study of P. mirabilis biology. However, the library's coverage of predicted genes was lower than expected due to unmapped insertions and a high proportion of mixed clones and multiple transposon insertion events. Future library construction can be improved by addressing these challenges.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Melanie M. Pearson, Allyson E. Shea, Sapna Pahil, Sara N. Smith, Valerie S. Forsyth, Harry L. T. Mobley
Summary: Animal models for host-microbial interactions are valuable but lacking for many microbes. This study introduces a method called organ agar, which enables screening of mutant libraries without physiological bottlenecks. The authors demonstrate that growth defects on organ agar accurately predict bacterial colonization deficiencies in a murine model. The method is also useful for identifying unknown links between biosynthetic genes and swarming motility.
INFECTION AND IMMUNITY
(2023)
