期刊
JOURNAL OF INFECTIOUS DISEASES
卷 200, 期 3, 页码 473-480出版社
OXFORD UNIV PRESS INC
DOI: 10.1086/600106
关键词
-
资金
- NCI NIH HHS [P30CA43703-12] Funding Source: Medline
- NIDCR NIH HHS [R01DE 13932-4, R01 DE017486-01A1] Funding Source: Medline
Background. The administration of parenteral nutrition, including lipid emulsion ( LE), to patients via medical catheters is an unexplained risk factor for the development of candidemia. Germination and biofilm formation are recognized virulence determinants of Candida albicans. No studies have addressed the effect of LE on candidal biofilm production. In this study, we investigated the effect of LE on candidal germination and its ability to form biofilm on medical catheter material. Methods. C. albicans strain SC-5314 was grown in standard growth medium in the presence or absence a commercially available LE. Biofilms grown on silicone-elastomer catheter discs in these media were compared for mass by dry weight measurements. Biofilm morphology was analyzed by scanning electron microscopy and confocal laser microscopy. The effect of LE on C. albicans germination and growth was evaluated microscopically and by determination of colony-forming units, respectively. Results. Addition of LE to standard growth medium increased C. albicans biofilm production and resulted in observed changes in biofilm morphology and architecture. Furthermore, LE induced germination and supported the growth of C. albicans. Conclusions. LE-inducible candidal virulence determinants, such as germination and enhanced biofilm production, may help to explain the increased risk of candidemia in patients receiving LE via medical catheters.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据