Article
Microbiology
Sohei Harada, Masahiro Suzuki, Toshiharu Sasaki, Aki Sakurai, Masato Inaba, Hosoda Takuya, Mitsutaka Wakuda, Yohei Doi
Summary: Carbapenemase-producing Escherichia coli strains were isolated from two international visitors without previous medical exposure in a hospital in Japan. The isolates carried different resistance genes and displayed high genetic similarity to strains isolated in South and Southeast Asian countries. This study highlights the need for active surveillance of travelers from countries with endemic carbapenem-resistant organisms, even in the absence of local hospitalization history.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Mustafa Sadek, Alaaeldin Mohamed Saad, Patrice Nordmann, Laurent Poirel
Summary: This article describes the phenotypic and genomic characteristics of an extensively drug-resistant E. coli strain isolated from the urine of a hospitalized patient in Switzerland. The strain is resistant to almost all beta-lactam antibiotics and carries multiple resistance genes. It is susceptible to tigecycline, colistin, and fosfomycin.
Article
Microbiology
Muhammad Shafiq, Mi Zeng, Budi Permana, Hazrat Bilal, Jinhu Huang, Fen Yao, Abdelazeem Mohamed Algammal, Xin Li, Yumeng Yuan, Xiaoyang Jiao
Summary: This study reports the first incident of a bla(NDM-5) and tet(X4) co-harboring Escherichia coli with resistance to carbapenem and tigecycline recovered as the causative agent of a urinary tract infection in a 94-year-old patient. The strain ECCL209 carries multiple resistance genes and exhibits resistance to almost all clinically used antibiotics. MLST analysis found that the strain belongs to the worldwide high-risk pandemic clone ST648, and multiple plasmid incompatibility types were detected.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Han Wang, Xuyong Li, Bao-Tao Liu
Summary: The study identified horses in an equestrian club in Qingdao, China carrying NDM-5 and KPC-2 producing bacteria, with plasmids capable of transferring between different bacterial species. The potential transmission risk of these CRE highlights the need for further monitoring of CRE prevalence among equestrian clubs nationwide.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Immunology
Agustina Costa, Roque Figueroa-Espinosa, Florencia Gaudenzi, Nilton Lincopan, Bruna Fuga, Barbara Ghiglione, Gabriel Gutkind, Jose Di Conza
Summary: This study describes the first clinical isolate of NDM-5- and RmtB-producing Escherichia coli in Latin America, displaying resistance to multiple antibiotics. The co-transfer of resistance genes located on conjugative plasmids poses a challenge for clinical treatment, and the spread of pathogens resistant to last resort antibiotics should raise an alarm in the healthcare system worldwide.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Patricia J. Simner, Heba H. Mostafa, Yehudit Bergman, Michael Ante, Tsigereda Tekle, Ayomikun Adebayo, Stephan Beisken, Kathryn Dzintars, Pranita D. Tamma
Summary: This study revealed the mechanisms by which New Delhi metallo-β-lactamase-producing Escherichia coli isolate developed relatively rapid resistance to cefiderocol through increased copy numbers of bla(NDM) genes. The findings highlight the importance of investigating increased bla(NDM-5) expression frequency in contributing to cefiderocol resistance.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Microbiology
Fengtian Li, Kun Ye, Xin Li, Liyan Ye, Ling Guo, Lifeng Wang, Jiyong Yang
Summary: This study investigated 144 carbapenem-resistant Escherichia coli (CREco) isolates from 10 cities in China, finding that the majority belonged to ST167, followed by ST410 and ST131; most of them carried various NDM-coding genes, with some ST types showing an outbreak trend.
Article
Microbiology
Zimeng Hu, Weiye Chen, Genglin Guo, Chen Dong, Yun Shen, Si Qin, Long Chen, Wei Zhang
Summary: The study identified a carbapenem-resistant Gram-negative bacterium AH001 with multidrug resistance isolated from hospital sewage. Whole-genome sequencing showed that AH001 could not be classified by existing MLST, and its serotype could not be distinguished among O9, O89 or O168 according to O antigen prediction. This highlights the importance of environmental sources of Escherichia coli in the development and transfer of drug resistance in the hospital setting.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Yan Guo, Yanan Yang, Xiangzhu Xu, Li Li, Yonglin Zhou, Guobin Jia, Lijuan Wei, Qinlei Yu, Jianfeng Wang
Summary: This study identifies fisetin as an effective inhibitor of NDM-1, reducing bacterial resistance to antibiotics. Combining fisetin with meropenem significantly attenuates resistance and shows synergistic effects in vitro and in vivo. Molecular simulations reveal the mechanism of fisetin's inhibitory activity on NDM-1. Fisetin represents a promising strategy for combating carbapenem-resistant bacterial infections.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Saoussen Oueslati, Cecile Emeraud, Victor Grosperrin, Marc Levy, Garance Cotellon, Elodie Creton, Lauraine Gauthier, Remy A. Bonnin, Thierry Naas, Laurent Dortet
Summary: The whole-genome sequencing analysis revealed a polyclonal dissemination of NDM-1 and NDM-9 variants in Escherichia coli and Klebsiella pneumoniae in Tahiti since 2015. There is a potential risk of importation of NDM producers in France, as French Polynesia is not considered a foreign country from which repatriated patients have to be screened.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Sijia Pan, Shuangqing Liu, Shuhong Tai, Jing Yu, Enwu Yuan, Yitao Duan
Summary: This study reports a pandrug-resistant E. coli ST167 isolate carrying multiple antibiotic resistance genes and the possibility of transmission between different species. Continued monitoring is necessary.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Maria Piekar, Veronica E. Alvarez, Camila Knecht, Carolina Leguina, Natalia Garcia Allende, Laura Carrera Paez, Anahi S. Gambino, Adrian Gonzalez Machuca, Josefina Campos, Barbara Fox, Eduardo Carpio, Andrea Aguilar, Fernando M. Alonso, Liliana Fernandez Canigia, Maria Paula Quiroga, Daniela Centron
Summary: This study investigated the genomic features of a multi-drug resistant strain of Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli. The strain carried 19 antibiotic resistance genes and had the potential for global dissemination.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Medicine, General & Internal
Ji-Na Gu, Lin Chen, Xing-Bei Weng, Xiao-Yan Yang, Dan-Mei Pan
Summary: The research revealed that Escherichia coli Huamei202001 belongs to sequence type 410 and carries a blaNDM-5-encoding IncX3-type plasmid responsible for carbapenem resistance spreading. Only the patient was detected as having a positive faecal sample screening test for CRE.
FRONTIERS IN MEDICINE
(2021)
Article
Microbiology
Trinad Chakraborty, Mustafa Sadek, Yancheng Yao, Can Imirzalioglu, Roger Stephan, Laurent Poirel, Patrice Nordmann
Summary: Clinical NDM-5-producing Escherichia coli isolates from Switzerland and Germany were highly resistant to beta-lactams but remained susceptible to certain antibiotics, mainly associated with the ST167 clone. The bla(NDM-5) gene was predominantly present on IncF plasmids with conserved genetic structures. This suggests a common genetic origin and international spread of this multidrug-resistant superbug, particularly associated with the ST167 clone.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Baomo Liu, Yingyi Guo, Ningjing Liu, Jiong Wang, Feifeng Li, Likang Yao, Chao Zhuo
Summary: IncX3 plasmids have been prevalent in China over the last 10 years, and are specifically associated with hospital-isolated Escherichia coli ST410. Despite the highly conservative backbone, the resistance genetic contexts on these plasmids could fall into different subtypes, indicating the spread of resistance gene environment across different bacterial genera. It is crucial to adopt stringent infection control measures to target IncX3 plasmid spread.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Hitoshi Yonezawa, Shingo Tanaka, Makito Tanaka, Ryo Kobayashi, Satoshi Takahashi
Summary: The aim of this study was to identify the risk factors for false positives in high-sensitivity HBsAg quantitative tests and reduce the need for neutralization tests. The results showed that younger age, female sex, lower HBsAg values, and reagent improvement were independent risk factors for false positives. The false-positive rate was highest in the range of 0.005-0.049 IU/mL.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2024)
Article
Infectious Diseases
Nobuaki Kobayashi, Katsushi Tanaka, Suguru Muraoka, Kohei Somekawa, Ayami Kaneko, Sousuke Kubo, Hiromi Matsumoto, Hiroaki Fujii, Keisuke Watanabe, Nobuyuki Horita, Yu Hara, Takeshi Kaneko
Summary: This study identified age, a negative T-SPOT.TB result, elevated CRP levels, and a high NLR as significant independent risk factors for death in hospitalized TB patients. These findings underscore the importance of these parameters in the risk stratification and management of hospitalized TB patients.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2024)
Article
Infectious Diseases
Naokuni Hishiya, Kenji Uno, Akiyo Nakano, Mitsuru Konishi, Seiya Higashi, Shuhei Eguchi, Tadashi Ariyoshi, Asami Matsumoto, Kentaro Oka, Motomichi Takahashi, Yuki Suzuki, Saori Horiuchi, Nobuyasu Hirai, Yoshihiko Ogawa, Taku Ogawa, Ryuichi Nakano, Keiichi Mikasa, Kei Kasahara, Hisakazu Yano
Summary: This study revealed intestinal dysbiosis near a CD4 count of 350 in HIV-infected patients undergoing cART. These findings contribute to the understanding of intestinal damage and systemic inflammation in HIV infection.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2024)
