期刊
JOURNAL OF INFECTION AND CHEMOTHERAPY
卷 15, 期 2, 页码 62-69出版社
ELSEVIER SCIENCE BV
DOI: 10.1007/s10156-009-0669-9
关键词
Loop-mediated isothermal amplification (LAMP); Nucleic acid amplification test; Rapid diagnosis tool; Point of care testing (POCT) device
Loop-mediated isothermal amplification (LAMP) is an established nucleic acid amplification method offering rapid, accurate, and cost-effective diagnosis of infectious diseases. This technology has been developed into commercially available detection kits for a variety of pathogens including bacteria and viruses. The current focus on LAMP methodology is as a diagnostic system to be employed in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The combination of LAMP and novel microfluidic technologies such as Lab-on-a-chip may facilitate the realization of genetic point-of-care testing systems to be used by both developed and developing countries in the near future. This review will describe the historical, current, and future developments of such technologies.
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