Article
Immunology
Ting Sun, Xiaojing Wu, Ying Cai, Tianshu Zhai, Linna Huang, Yi Zhang, Qingyuan Zhan
Summary: The study compared the pathogenic diagnosis performance of immunocompromised and immunocompetent patients with severe community-acquired pneumonia (SCAP) using metagenomic next-generation sequencing (mNGS). It was found that immunocompromised patients had a higher rate of mixed pathogens and required more antibiotic adjustments compared to immunocompetent patients. Metagenomic next-generation sequencing (mNGS) may be a useful technique for detecting mixed pathogens and guiding personalized antibiotic treatment in immunocompromised SCAP patients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Zhenli Diao, Dongsheng Han, Rui Zhang, Jinming Li
Summary: This review summarizes the potential applications and challenges of metagenomic next-generation sequencing (mNGS) in the diagnosis of lower respiratory tract infections (LRIs), aiming to promote the integration of mNGS into the management of patients with respiratory tract infections in a clinical setting.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Public, Environmental & Occupational Health
Quan Zhang, Wei Xu, Fei Peng, Si Lei, Yanjun Zhong, Jun Yi, Shangjie Wu
Summary: A case of aspiration pneumonia with radiological and clinical similarities to COVID-19 was reported. The study highlights the importance of careful examination of CT images, attention to clinical history, and judicious utilization of NGS for accurate diagnosis.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Infectious Diseases
Shixiao Li, Peng Zhou, Lihong Yang, Tianbin Tang, Jiajia Qin, Jiao Qian, Shen Bo, Sufei Yu
Summary: This study aims to evaluate the clinical value of the sampling time of mNGS in patients with severe pneumonia. The results showed that early sampling can significantly shorten the mechanical ventilation time and hospitalization time. The study emphasizes the positive significance of early sampling.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Junli Tang, Wanmei Tan, Lingxin Luo, Huan Xu, Na Li
Summary: Psittacosis pneumonia is easily underdiagnosed and misdiagnosed. This study uses mNGS in the diagnosis of psittacosis pneumonia. It is prone to occur in the cold season, and touching the innards of killed poultry during cooking may be an atypical exposure history that has not been reported before. There are sporadic cases and family outbreak cases as well. In addition to typical symptoms of fever and weakness, headache may be the main and only symptom in some patients. The rate of severe pneumonia is high among inpatients with psittacosis pneumonia, and the incidence of hepatic involvements is also high. Psittacosis pneumonia can be cured if the diagnosis is accurate and timely, even if it is severe pneumonia on admission. Some worthy attention about psittacosis pneumonia were put forward, such as its sick season, special exposure history, the rate of severe disease, and the high cure rate. mNGS can quickly and objectively detect more rare pathogenic microorganisms in clinical specimens without the need for specific amplification and has an advantage in the diagnosis of rare pathogenic bacteria in difficult cases such as psittacosis pneumonia. The use of mNGS can improve the accuracy and reduce the delay in the diagnosis of psittacosis, which shortens the course of disease control.
MICROBIOLOGY SPECTRUM
(2022)
Review
Immunology
Minjie Lv, Changjun Zhu, Chenghua Zhu, Jing Yao, Lixu Xie, Changwen Zhang, Jianling Huang, Xingran Du, Ganzhu Feng
Summary: This meta-analysis found that metagenomic next-generation sequencing (mNGS) can significantly increase the pathogen detection rate and improve the prognosis of patients with severe pneumonia compared to conventional methods. Adjusted treatment based on mNGS results can reduce the 28-day and 90-day mortality and shorten the length of hospital and ICU stay.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Yaoguang Li, Jun Lei, Zhigang Ren, Xiaoxu Ma
Summary: This article discusses a case report of a patient with severe community-acquired pneumonia, who was successfully treated with rapid mNGS testing and ECMO therapy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Public, Environmental & Occupational Health
Huifen Wang, Su Yan, Ying Liu, Yaoguang Li, Guangying Cui, Xiaoxu Ma
Summary: Through research conducted from 2019 to 2020, it was found that mNGS can be used as an auxiliary method for PC diagnosis. Early mNGS can help in the early identification of pathogens, enabling early diagnosis and treatment, as well as reducing the misdiagnosis rate and disease progression.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Immunology
Yueming Liang, Tingyan Dong, Minjing Li, Peifang Zhang, Xiaoqun Wei, Haitao Chen, Yongsi Wang, Xinglin Gao
Summary: The incidence of severe Chlamydia psittaci pneumonia and coinfections is increasing. Early detection using metagenomic next-generation sequencing (mNGS) can accurately detect C. psittaci and other pathogens, as well as identify coinfections for better clinical treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Hongqin Zhao, Yanhong Zhao, Nana Yan, Yu Wang, Wushuang Li, Jiangman Zhao, Yue Xu, Hui Tang, Xunchao Liu
Summary: This study investigated the diagnostic performance of mNGS on BALF samples from individuals suspected of pneumonia and compared it with conventional microbiological tests and clinical diagnosis. The results showed that mNGS represents a potentially effective tool for detection of mixed pathogens in severe pneumonia.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Medicine, Research & Experimental
Xiaohui Zhang, Yuhong Qin, Wei Lei, Jian-An Huang
Summary: The diagnostic accuracy of metagenomic next-generation sequencing (mNGS) is superior to conventional microbiological tests (CMTs) for severe community-acquired pneumonia (SCAP) in immunocompromised patients. mNGS has advantages in detecting polymicrobial infections and identifying unexpected pathogens. Therefore, mNGS can be considered as an important diagnostic method for SCAP.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Kaiying Wang, Xiong Liu, Huiying Liu, Peihan Li, Yanfeng Lin, Dongdong Yin, Lang Yang, Jinhui Li, Shenlong Li, Leili Jia, Changqing Bai, Yongqiang Jiang, Peng Li, Hongbin Song
Summary: Metagenomic sequencing can rapidly diagnose unknown infections and provide additional information on antibiotic susceptibility. Optimizing and standardizing sampling and metagenomic analysis has the potential to improve the clinical utility of genomic diagnosis.
Article
Microbiology
Hengxin Chen, Yujian Liang, Ruizhi Wang, Yijie Wu, Xiaoyun Zhang, Hao Huang, Xuegao Yu, Mengzhi Hong, Juhua Yang, Kang Liao, Hongxu Xu, Min Liu, Peisong Chen, Yili Chen
Summary: The study evaluated the effectiveness of metagenomic next-generation sequencing (mNGS) for diagnosing Pneumocystis jirovecii Pneumonia (PCP) in critically pediatric patients. The results showed that mNGS had a sensitivity of 100% in detecting P. jirovecii, outperforming conventional methods and other diagnostic indicators. mNGS had a high specificity for PCP and could detect mixed infections. The study suggests that mNGS is a potential and efficient technology for diagnosing PCP.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Immunology
Na Li, Shengjin Li, Wanmei Tan, Hanghang Wang, Huan Xu, Daoxing Wang
Summary: This study utilized metagenomic next-generation sequencing to diagnose a family outbreak of psittacosis during the COVID-19 pandemic, revealing the challenges in distinguishing psittacosis pneumonia from CAP caused by other pathogens based on clinical manifestations and imaging findings. The use of mNGS improved accuracy and reduced diagnostic delays, particularly during outbreaks, potentially shortening disease control measures.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Respiratory System
Hu Li, Binwei Hao, Yongxue Wang, Dinghong Yu, Zhonghua Chen, Duanlin Du, Jian Xiong, Kang Li, Hengping Zhang, Xu Liu, Kai Liu, Fanrong Xiao, Xiaosan Cheng, Lingmei Huang
Summary: This study investigated the clinical characteristics of Chlamydia psittaci pneumonia and evaluated the diagnostic value of mNGS. Results showed that most patients had a history of exposure to poultry or birds and presented with fever. Apart from systemic and respiratory symptoms, some patients also presented with digestive and neurological symptoms. The prognosis was poor for patients with severe pneumonia, and mNGS was found to be an effective diagnostic method.
CLINICAL RESPIRATORY JOURNAL
(2022)
