Article
Biochemistry & Molecular Biology
Ruihao Zhong, Junbin Wei, Bin Liu, Honghui Luo, Zhaoqi Zhang, Xuequn Pang, Fang Fang
Summary: This study identified the major proanthocyanidins (PAs) in litchi pericarp as (-)-epicatechin (EC) and several procyanidins (PCs). The EC and PC content decreased with fruit development. Nine PA biosynthesis-related genes were identified, including ANR1a, LAR1/2, and ANS1, which were highly correlated with changes in EC/PC content. Transcription factor genes such as MYB, bHLH, WRKY, and AP2 were found to play a regulatory role in PA biosynthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Virginia Diez-Obrero, Christopher H. Dampier, Ferran Moratalla-Navarro, Matthew Devall, Sarah J. Plummer, Anna Diez-Villanueva, Ulrike Peters, Stephanie Bien, Jeroen R. Huyghe, Anshul Kundaje, Gemma Ibanez-Sanz, Elisabeth Guino, Mireia Obon-Santacana, Robert Carreras-Torres, Graham Casey, Victor Moreno
Summary: This study characterized gene expression and splicing profiles across different colon subsites, identified numerous expression and splicing QTLs, proposed candidate effector genes for diseases affecting colon tissue and other tissues, and developed the Colon Transcriptome Explorer web application for visualization. The findings provide valuable insights into the genetic underpinnings of complex traits and diseases influenced by transcriptomic changes in colon tissue.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Endocrinology & Metabolism
Ya Hu, Xiang Zhang, Ou Wang, Ming Cui, Xiaobin Li, Mengyi Wang, Surong Hua, Quan Liao
Summary: Mutations in genes such as MEN1, mTOR, ZFX, CASR, EZH2, and FAT1 were commonly found in parathyroid adenoma (PA) patients, with a novel EZH1 somatic mutation also being discovered. RNA-seq data revealed dysregulation of numerous genes in PA samples compared to normal tissue samples. Unsupervised clustering analysis grouped patients into 10 subgroups related to mutations or abnormal expression of potential pathogenic genes.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Immunology
Kathryn A. A. Recto, Tianxiao Huan, Dong Heon Lee, Gha Young Lee, Jessica Gereige, Chen Yao, Shih-Jen Hwang, Roby Joehanes, Rachel S. S. Kelly, Jessica Lasky-Su, George O'Connor, Daniel Levy
Summary: Measurement of circulating immunoglobulin E (IgE) concentration is helpful for diagnosing and treating asthma and allergic diseases. Identifying gene expression signatures associated with IgE might elucidate novel pathways for IgE regulation. Our findings build upon prior knowledge of IgE regulation and provide a deeper understanding of underlying molecular mechanisms, offering promising therapeutic targets for asthma and IgE-related diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Pooja Wakchoure, Khizra Momin, Abdul Arif Khan
Summary: Tuberculosis (TB) is a significant health burden for patients with human immunodeficiency virus (HIV) due to immunosuppression and susceptibility. This study analyzed gene expression data from TB-infected macrophages and blood samples from TB and HIV patients to identify genes associated with both diseases. Host targets, including PLSCR1, STAT1, FBXO6, ITGAL, and APP, were found to be commonly modulated in both HIV and TB. These targets may play important roles in the pathogenesis of this comorbidity and further research is needed for therapeutic innovation.
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Elisa Padoan, Serena Ferraresso, Sara Pegolo, Carlo Barnini, Massimo Castagnaro, Luca Bargelloni
Summary: This study comprehensively defined the transcriptomic fingerprints of mild and severe equine asthma using gene expression analysis. The pathogenesis of equine asthma involves reduced mucociliary clearance and excessive complement activation leading to tissue injury. Furthermore, severe equine asthma is characterized by chronic inflammation and tissue remodeling similar to human asthma.
Article
Medicine, General & Internal
Arjun Chandna, Endashaw M. Aderie, Riris Ahmad, Eggi Arguni, Elizabeth A. Ashley, Tanya Cope, Vu Quoc Dat, Nicholas P. J. Day, Arjen M. Dondorp, Victor Illanes, Joanne De Jesus, Carolina Jimenez, Kevin Kain, Keang Suy, Constantinos Koshiaris, Estrella Lasry, Mayfong Mayxay, Dinesh Mondal, Rafael Perera, Tiengkham Pongvongsa, Sayaphet Rattanavong, Michael Rekart, Melissa Richard-Greenblatt, Mohammad Shomik, Phouthalavanh Souvannasing, Veronica Tallo, Claudia Turner, Paul Turner, Naomi Waithira, James A. Watson, Mikhael Yosia, Sakib Burza, Yoel Lubell
Summary: This study aims to develop a prognostic clinical prediction tool to assist healthcare providers in identifying febrile children who may benefit from referral or admission for medical care. The study will recruit at least 4900 paediatric inpatients and outpatients under the age of 5 with acute febrile illness in seven hospitals across six countries in Asia.
Article
Immunology
Ming-Wei Jan, Hong-Lin Su, Tsung-Hsien Chang, Kuen-Jer Tsai
Summary: Human parechovirus type 3 (PeV-A3) infection is identified as a cause of severe nerve disease or sepsis in infants and young children, with neuropathogenic mechanisms involving interferon and inflammatory cytokine expression, activation of death signaling pathways, and establishment of an experimental disease model of parechovirus encephalitis. In vivo studies in neonatal mice showed weight loss, paralysis, and mortality, with evidence of PeV-A3 infection, inflammatory cytokine expression, and death signaling transduction in the brain tissues. These findings suggest that PeV-A3-mediated neurological disorders involve activation of death signaling and inflammation responses, which may contribute to clinical manifestations associated with PeV-A3 infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
AbdulRahman A. Saied, Manish Dhawan, Asmaa A. Metwally, Mathumalar Loganathan Fahrni, Priyanka Choudhary, Om Prakash Choudhary
Summary: Monkeypox is a zoonotic disease that spreads between animals and humans. The current outbreak is not related to travel and mainly spreads through close contact and exposure to contaminated materials. Diagnosis of monkeypox relies on nucleic-acid amplification testing, while other methods are time-consuming. There is no specific treatment for monkeypox, but vaccinia vaccine and certain drugs used for smallpox may be beneficial.
Article
Biochemistry & Molecular Biology
Maximilien Franck, Juan de Toro-Martin, Thibault V. Varin, Veronique Garneau, Genevieve Pilon, Denis Roy, Patrick Couture, Charles Couillard, Andre Marette, Marie-Claude Vohl
Summary: Numerous studies have found that diets rich in phenolic compounds are beneficial for immune-metabolic health, but the effects vary among individuals and the mechanisms are not well understood. This study used transcriptomic analysis to identify two subgroups of responders and non-responders to raspberry consumption based on whole-blood RNAseq data. Responders showed a significant decrease in triglycerides, cholesterol, and C-reactive protein levels, and had distinct gene expression and microbiota profiles compared to non-responders. The transcriptomic approach used in this study provides a promising framework for understanding the inter-individual variability in the impact of foods on immune-metabolic health.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Emergency Medicine
Preston Dean, Daniel Schumacher, Todd A. Florin
Summary: This study developed expert consensus on factors associated with severity in pediatric CAP through a Delphi process, identifying important clinical, radiographic, and laboratory factors as well as outcomes. The majority of items reached consensus, providing a foundation for future development of risk stratification scores in pediatric CAP.
PEDIATRIC EMERGENCY CARE
(2021)
Review
Infectious Diseases
Alexandra Binnie, Emanuel Fernandes, Helder Almeida-Lousada, Ramon Andrade de Mello, Pedro Castelo-Branco
Summary: CRISPR gene-editing technology has the potential to revolutionize the diagnosis and treatment of infectious diseases, offering novel approaches such as rapid diagnostic systems, drug-resistance gene identification, and targeted therapies against resistant bacteria and emerging viruses like SARS-CoV-2. Despite risks like off-target modifications, strategies are being developed to control these risks, with ongoing phase 1 clinical trials for CRISPR-based therapies for cancer and monogenic diseases.
Article
Multidisciplinary Sciences
Xiaoyao Chang, Zhongren Yang, Xiaoyan Zhang, Fenglan Zhang, Xiumei Huang, Xu Han
Summary: This study is the first to identify the WRKY gene family in Cynanchum thesioides. The classification and analysis of the genes provide a foundation for further understanding their functions in the growth, development, and responses of Cynanchum thesioides.
Article
Multidisciplinary Sciences
Aili Saiding, Dilinuer Maimaitiyiming, Minglan Chen, Futian Yan, Dong Chen, Xinyu Hu, Ping Shi
Summary: This study investigates the cellular functions of PCMT1 in TNBC cells and identifies its molecular targets through RNA-seq. Silencing PCMT1 inhibits cell proliferation, migration, and invasion in TNBC. The dysregulated genes and alternative splicing events associated with PCMT1 provide new insights into the understanding of TNBC progression. The identified molecular targets could be potential therapeutic targets for TNBC treatment.
Article
Agriculture, Dairy & Animal Science
Zhixiu Wang, Wenshuang Liang, Dan Yan, Huiyue Tian, Bingqiang Dong, Wenming Zhao, Guobin Chang, Guohong Chen
Summary: This study identified candidate genes related to duck skeletal muscle growth using RNA-sequencing technology and found that these genes are mainly involved in biological processes and pathways of fat metabolism and muscle growth. These findings deepen our understanding of the molecular mechanisms involved in muscle growth in ducks.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Immunology
Helena Brenes-Chacon, Cristina Garcia-Maurino, Melissa Moore-Clingenpeel, Sara Mertz, Fang Ye, Daniel M. Cohen, Octavio Ramilo, Asuncion Mejias
Summary: This study investigated RSV infection in infants and young children, finding significant correlations between age, viral loads, and clinical manifestations, as well as differences in disease severity among patients of different age groups. Adjusted analyses revealed that respiratory distress and fever were closely related to hospitalization, while infantile wheezing and higher RSV loads were associated with reduced disease severity.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2021)
Article
Immunology
Karoliina Koivisto, Tea Nieminen, Asuncion Mejias, Cristina Capella Gonzalez, Fang Ye, Sara Mertz, Mark Peeples, Octavio Ramilo, Harri Saxen
Summary: Maternal pre-F antibodies are essential for providing immune protection to infants against RSV bronchiolitis.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Alejandro Diaz-Diaz, Eleonora Bunsow, Cristina Garcia-Maurino, Melissa Moore-Clingenpeel, Jeffrey Naples, Alexis Juergensen, Sara Mertz, Huanyu Wang, Amy L. Leber, James Gern, Mark W. Hall, Daniel M. Cohen, Octavio Ramilo, Asuncion Mejias
Summary: The study found that detection of nasopharyngeal bacteria, specifically S. pneumoniae and H. influenzae, in infants with RSV infection is associated with increased disease severity and worse clinical outcomes.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Raquel Giacomelli Cao, Lisa Christian, Zhaohui Xu, Lisa Jaramillo, Bennett Smith, Erik A. Karlsson, Stacey Schultz-Cherry, Asuncion Mejias, Octavio Ramilo
Summary: Influenza vaccination in pregnant women led to upregulation of interferon-stimulated genes on day 1 and plasma cell genes on day 7, which were significantly correlated with increased antibody titers. Compared to non-vaccinated women, those vaccinated during the previous influenza season showed more frequent significant correlations between ISGs and antibody titers.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Editorial Material
Pediatrics
Asuncion Mejias, Maria Sanchez-Codez, Octavio Ramilo, Pablo J. Sanchez
JORNAL DE PEDIATRIA
(2022)
Article
Pediatrics
Sabrina Palacios, Katelyn Krivchenia, Mariah Eisner, Bailey Young, Octavio Ramilo, Asuncion Mejias, Simon Lee, Benjamin T. Kopp
Summary: This study is the largest to date on long-term pulmonary sequelae in pediatric patients after COVID-19. Identified clinical phenotypes and risk factors require further study and treatment.
PEDIATRIC PULMONOLOGY
(2022)
Article
Environmental Sciences
Guisselle Arias-Bravo, Gustavo Valderrama, Jaime Inostroza, Cecilia Tapia, Daniela Toro-Ascuy, Octavio Ramilo, Paz Orellana, Nicolas Cifuentes-Munoz, Francisco Zorondo-Rodriguez, Asuncion Mejias, Loreto F. Fuenzalida
Summary: This study evaluated the association between nasopharyngeal bacterial profile and nutritional status in children with viral lower respiratory tract infections. The results showed that overnutrition was associated with a higher presence and load of pathogenic bacteria in the nasopharynx, as well as increased proinflammatory cytokine concentrations.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Immunology
Jeanette Taveras, Cristina Garcia-Maurino, Melissa Moore-Clingenpeel, Zhaohui Xu, Sara Mertz, Fang Ye, Phyl Chen, Shira H. Cohen, Daniel Cohen, Mark E. Peeples, Octavio Ramilo, Asuncion Mejias
Summary: In this study, children with milder RSV infection and those aged >6 months had higher concentrations of mucosal interferons, which may be associated with protection against severe RSV disease. Higher levels of IL6 increased the likelihood of hospitalization in older children, while higher concentrations of IFN-lambda 2/3 had the opposite effect regardless of age. Additionally, higher concentrations of CXCL10 were associated with reduced odds of hospitalization, oxygen administration, PICU admission, and prolonged hospitalization.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Letter
Pediatrics
Katia C. Halabi, Huanyu Wang, Amy L. Leber, Pablo J. Sanchez, Octavio Ramilo, Asuncion Mejias
PEDIATRIC PULMONOLOGY
(2022)
Editorial Material
Immunology
Octavio Ramilo, Rosa Rodriguez-Fernandez, Asuncion Mejias
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Pediatrics
Asuncion Mejias, Julia Schuchard, Suchitra Rao, Tellen D. Bennett, Ravi Jhaveri, Deepika Thacker, L. Charles Bailey, Dimitri A. Christakis, Nathan M. Pajor, Hanieh Razzaghi, Christopher B. Forrest, Grace M. Lee
Summary: By using an electronic health record-based algorithm, researchers identified serology-positive children with COVID-19 between March 2020 and April 2022. Compared to PCR-positive children, serology-positive children were more likely to be hospitalized, have chronic conditions, and be diagnosed with multisystem inflammatory syndrome in children. Identifying asymptomatic or paucisymptomatic children who were not tested is crucial for understanding the burden of post-acute sequelae of SARS-CoV-2 infection in children.
JOURNAL OF PEDIATRICS
(2023)
Article
Infectious Diseases
Huanyu Wang, Tori Wright, Kathy Everhart, Sophonie J. Oyeniran, Asuncion Mejias, Amy L. Leber
Summary: Beginning in late 2021, there was a significant increase in SARS-CoV-2 reinfections in pediatric patients evaluated at our institution. We aimed to characterize the children with SARS-CoV-2 reinfection, determine the number of SARS-CoV-2 reinfections, and characterize the intervals between two infections in our patient population.
JOURNAL OF THE PEDIATRIC INFECTIOUS DISEASES SOCIETY
(2023)
Article
Health Care Sciences & Services
Vitaly Lorman, Suchitra Rao, Ravi Jhaveri, Abigail Case, Asuncion Mejias, Nathan M. Pajor, Payal Patel, Deepika Thacker, Seuli Bose-Brill, Jason Block, Patrick C. Hanley, Priya Prahalad, Yong Chen, Christopher B. Forrest, L. Charles Bailey, Grace M. Lee, Hanieh Razzaghi
Summary: Pediatric long COVID in children is not clearly defined, but this study identified several symptoms and conditions associated with it by comparing diagnosed and undiagnosed children. These findings can be used to develop a precise definition of long COVID in children for future studies.
Article
Infectious Diseases
Ravi Jhaveri, Ryan Webb, Hanieh Razzaghi, Julia Schuchard, Asuncion Mejias, Tellen D. Bennett, Pei-Ni Jone, Deepika Thacker, Grant S. Schulert, Colin Rogerson, Jonathan D. Cogen, L. Charles Charles Bailey, Christopher B. Forrest, Grace M. Lee, Suchitra Rao, RECOVER consortium
Summary: Using electronic health record data and primary chart review, this study identified seven children with Multisystem Inflammatory Syndrome in Children (MIS-C) who were managed as outpatients across nine pediatric medical centers. These findings highlight the importance of recognizing mild presentations of MIS-C and the possibility of outpatient management.
JOURNAL OF THE PEDIATRIC INFECTIOUS DISEASES SOCIETY
(2023)
Review
Medical Laboratory Technology
David Bernstein, Asuncion Mejias, Barbara Rath, Christopher W. Woods, Jamie Phillips Deeter
Summary: This scoping literature review summarizes the study characteristics of commercially available sample-to-answer RSV tests. The review reveals a lack of literature on studies of RSV tests in specific populations and settings, highlighting the need for further assessments. Molecular-based rapid RSV tests are suggested as first-line use in the current pandemic landscape due to their higher sensitivity and specificity.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2023)
