Article
Microbiology
Maria Kulecka, Edyta Waker, Filip Ambrozkiewicz, Agnieszka Paziewska, Karolina Skubisz, Patrycja Cybula, Lukasz Targonski, Michal Mikula, Jan Walewski, Jerzy Ostrowski
Summary: Our study revealed that the presence of specific genes related to carbon metabolism and oxidative phosphorylation increased the risk of infection recurrence. More core genes were found to be under positive selective pressure in recurrent disease isolates, mainly associated with amino acid metabolism. Prophage elements were more prevalent in single infection isolates, while plasmids did not influence the odds of recurrence.
Article
Biochemistry & Molecular Biology
Laura Tijerina-Rodriguez, Elvira Garza-Gonzalez, Adrian Martinez-Melendez, Rayo Morfin-Otero, Adrian Camacho-Ortiz, Esteban Gonzalez-Diaz, Hector Raul Perez-Gomez, Licet Villarreal-Trevino, Hector Maldonado-Garza, Sergio Esparza-Ahumada, Eduardo Rodriguez-Noriega
Summary: This study evaluated risk factors associated with severe-complicated and recurrent outcomes in different age groups of Clostridium difficile infection (CDI) patients. Female gender and lymphoma were identified as risk factors for severe-complicated CDI, while mature adulthood, previous rifampicin use, and neoplasm were risk factors for recurrent infection. Autoimmune disorders, leukemia, lymphoma, and previous colistin treatment were found to be risk factors for 30-day mortality.
BIOMEDICAL JOURNAL
(2022)
Article
Medicine, General & Internal
Maria Martinez-Urbistondo, Angela Gutierrez-Rojas, Ane Andres, Isabel Gutierrez, Gabriela Escudero, Sonia Garcia, Andrea Gutierrez, Enrique Sanchez, Jesus Herraiz, Sara De La Fuente, Alejandro Callejas, Carmen De Mendoza, Victor Moreno-Torres
Summary: Immunosuppression is an independent risk factor for mortality in COVID-19 patients, with severe lymphopenia playing a key role in immunocompromised individuals. Prompt identification of severe lymphopenia is critical in these patients. Immunosuppression remains significantly associated with a higher mortality rate even after adjustment for other factors.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Jianfeng Wang, Chu Yang, Chao Zhang, Xiaoyan Mao, An Lizhe
Summary: In this study, the genome of Clostridium difficile was studied using metagenomic technology, revealing its gene sequence information and potential toxicological genes. Through gene function analysis and pathogenicity analysis, the metabolism activity, catalytic activity, carbohydrate-active enzyme genes and other aspects of the bacterium were understood, and an assessment was made on its virulence genes.
Review
Microbiology
Marta Mattana, Riccardo Tomasello, Claudia Cammarata, Paola Di Carlo, Teresa Fasciana, Giulio Giordano, Alessandro Lucchesi, Sergio Siragusa, Mariasanta Napolitano
Summary: This review analyzed the correlation between coagulation complications related to C. difficile infection and inflammasome activation, particularly the pyrin-dependent one. The evidence suggests that inflammasome activation increases the risk of venous thromboembolism, and inhibition of molecules involved in coagulation activation could block this process. It may be possible to reduce complications and mortality associated with C. difficile infection by reducing inflammatory activity and preventing thromboembolic complications.
Review
Medicine, General & Internal
Ibrahim A. Al-Zahrani
Summary: Clostridioides difficile (C. difficile) infection is a global threat to many healthcare settings. Excessive use of antimicrobials, lack of optimal antibiotic policies, and suboptimal infection control practices have fueled the development of this health issue. Prudent use of antimicrobials and simple infection control measures can significantly reduce infection rates.
SAUDI MEDICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Srijita Basak, Debashrito Deb, Utkarsh Narsaria, Tamalika Kar, Filippo Castiglione, Indraneel Sanyal, Pratap D. Bade, Anurag P. Srivastava
Summary: Clostridium difficile infection is a major health-associated infection with high incidence and mortality rates. A chimeric vaccine candidate was designed using immunoinformatics, which showed stability and reliability in silico and molecular dynamics simulations. Docking studies demonstrated stable interactions with immune receptors, and in silico modeling indicated competent expression in E. coli system for potential immune response.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Peng An Khun, Long Duc Phi, Huong Thi Thu Bui, Nguyen Thi Bui, Quyen Thi Huyen Vu, Luong Duy Trinh, Deirdre A. Collins, Thomas Riley
Summary: This study investigated the prevalence, molecular type, and antimicrobial susceptibility of Clostridioides difficile in the environment in Vietnam. It found that the overall contamination rate of C. difficile was 24.5%, with the highest prevalence in pig farm and hospital soils. C. difficile was also found in pig feces and potato surfaces. These findings highlight the importance of environmental sources in the epidemiology of C. difficile infection in Vietnam.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Jiuxing Li, Jimmy Gu, Hongfen Zhang, Rudi Liu, Wenqing Zhang, Mostafa Mohammed-Elsabagh, Jianrun Xia, Devon Morrison, Sandy Zakaria, Dingran Chang, Amjad Arrabi, Yingfu Li
Summary: A highly specific DNA aptamer for RNase H2 from Clostridium difficile was developed through SELEX, demonstrating its potential for diagnostic tests and drug development.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Meng Wang, Zifeng Deng, Yanmei Li, Yi Ma, Jufang Wang
Summary: A novel lytic protein Cw1-CWB2 with high binding specificity and strong lytic activity has been designed in this study for combating C. difficile.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ashleigh S. Paparella, Briana L. Aboulache, Rajesh K. Harijan, Kathryn S. Potts, Peter C. Tyler, Vern L. Schramm
Summary: Clostridium difficile produces toxins TcdA and TcdB causing diarrhea by affecting host cell functions using UDP-glucose, leading to disease onset. Researchers identified potential therapeutic approach using transition state analogue iminosugars.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, General & Internal
Camelia Cojocariu, Irina Girleanu, Anca Trifan, Andrei Olteanu, Cristina Maria Muzica, Laura Huiban, Stefan Chiriac, Ana Maria Singeap, Tudor Cuciureanu, Catalin Sfarti, Carol Stanciu
Summary: This study found that factors such as age, urban residence, history of antibiotic use, hospitalizations, and alcohol consumption were risk factors for the development of CDI in COVID-19 patients.
WORLD JOURNAL OF CLINICAL CASES
(2021)
Article
Pediatrics
Ryan M. Hanson, Amy J. Wagner
Summary: Severe, complicated Clostridium difficile colitis is rare in pediatric patients and little is known about its medical and surgical management in this population. A case report of a 15-year-old female patient with severe, complicated C difficile colitis successfully treated with diverting loop ileostomy and colonic lavage is presented. This approach, instead of subtotal colectomy and end ileostomy, should be considered as an alternative for pediatric patients with severe, complicated C difficile colitis.
Review
Medicine, General & Internal
Gowthami Sai Kogilathota Jagirdhar, Salim Surani
Summary: Pseudomembranous colitis is a severe inflammation of the colon caused by various factors, with Clostridium difficile being the most common cause. Symptoms include abdominal pain, diarrhea, fever, and dehydration. Other than C. difficile, other pathogens, medications, and diseases should be considered as differential diagnoses. Complications can be serious, and early diagnosis and treatment are crucial.
WORLD JOURNAL OF CLINICAL CASES
(2023)
Article
Pharmacology & Pharmacy
Giuseppe Esposito, Chiara Corpetti, Marcella Pesce, Luisa Seguella, Giuseppe Annunziata, Alessandro Del Re, Martina Vincenzi, Roberta Lattanzi, Jie Lu, Walter Sanseverino, Giovanni Sarnelli
Summary: The newly designed PEA-producing probiotic showed therapeutic potential in inhibiting colonic inflammation and restoring tight junction protein expression in a mouse model of CDI.
FRONTIERS IN PHARMACOLOGY
(2021)