Article
Immunology
Li Liu, Mingjuan Yuan, Yi Shi, Xin Su
Summary: The study evaluated the performance of BAL mNGS and serum BDG in differentiating P. jirovecii colonisation and infection, finding that they are useful adjunct tests for this purpose.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Critical Care Medicine
Daniele Roberto Giacobbe, Silvia Dettori, Vincenzo Di Pilato, Erika Asperges, Lorenzo Ball, Enora Berti, Ola Blennow, Bianca Bruzzone, Laure Calvet, Federico Capra Marzani, Antonio Casabella, Sofia Choudaly, Anais Dartevel, Gennaro De Pascale, Gabriele Di Meco, Melissa Fallon, Louis-Marie Galerneau, Miguel Gallego, Mauro Giacomini, Adolfo Gonzalez Saez, Luise Haensel, Giancarlo Icardi, Philipp Koehler, Katrien Lagrou, Tobias Lahmer, P. Lewis White, Laura Magnasco, Anna Marchese, Cristina Marelli, Mercedes Marin-Arriaza, Ignacio Martin-Loeches, Armand Mekontso-Dessap, Malgorzata Mikulska, Alessandra Mularoni, Anna Nordlander, Julien Poissy, Giovanna Russelli, Alessio Signori, Carlo Tascini, Louis-Maxime Vaconsin, Joel Vargas, Antonio Vena, Joost Wauters, Paolo Pelosi, Jean-Francois Timsit, Matteo Bassetti
Summary: This study describes the characteristics of ICU patients undergoing diagnostic workup for PJP, and finds that PJP is most common in non-HIV patients. The results also suggest that combining serum BDG with respiratory Pneumocystis PCR can improve the accuracy of PJP diagnosis.
Article
Microbiology
Dora Pungan, Taylor Eddens, Kejing Song, Meredith A. Lakey, Nicolle S. Crovetto, Simran K. Arora, Shahid Husain, Jay K. Kolls
Summary: Pneumocystis jirovecii is an important etiological agent of pneumonia, but its diagnosis often goes undetected. A newly developed NGS-based genotyping assay can be used for genotyping Pneumocystis and provides a more accurate method for non-bronchoscopic diagnosis.
Article
Biochemistry & Molecular Biology
Nina Tsvetkova, Rumen Harizanov, Iskra Rainova, Aleksandra Ivanova, Nina Yancheva-Petrova
Summary: This study investigated the presence of DHPS gene mutations among P. jirovecii isolates from Bulgarian patients with PCP. The results showed that infections caused by P. jirovecii mutants potentially resistant to sulfonamides are still rare events in Bulgaria, with DHPS genotype 1 being the predominant strain in the country.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Shuhei Ideguchi, Kazuko Yamamoto, Tatsuro Hirayama, Takahiro Takazono, Yoshifumi Imamura, Taiga Miyazaki, Noriho Sakamoto, Koichi Izumikawa, Katsunori Yanagihara, Shimpei Morimoto, Hiroshi Mukae
Summary: This study evaluated the diagnostic performance of serum (1, 3)-beta-D-glucan (BDG) levels using the Fungitec G-test MK kit for Pneumocystis jirovecii pneumonia (PCP) in non-HIV patients, showing that the serum BDG test was useful for diagnosing PCP but results should be interpreted carefully in case of methotrexate (MTX) administration.
Article
Medicine, General & Internal
Xiaoxiao Lu, Jianhui Zhang, Wentao Ma, Lihua Xing, Hanbing Ning, Mengying Yao
Summary: The incidence of non-HIV-infected Pneumocystis Jirovecii Pneumonia (PJP) is increasing. This study found that metagenomic next-generation sequencing (mNGS) can diagnose PJP quickly and accurately, and has advantages over traditional detection methods. However, PJP can easily be ignored due to atypical symptoms and ambiguous imaging features.
FRONTIERS IN MEDICINE
(2022)
Article
Infectious Diseases
Erica Franceschini, Giovanni Dolci, Antonella Santoro, Marianna Meschiari, Alice Ricco, Marianna Menozzi, Giulia Jole Burastero, Biagio Cuffari, Nicola De Maria, Lucia Serio, Emanuela Biagioni, Barbara Catellani, Stefano Di Sandro, Antonio Colecchia, Massimo Girardis, Fabrizio Di Benedetto, Cristina Mussini
Summary: This study aims to describe a case series of patients with decompensated liver cirrhosis and Pneumocystis jirovecii pneumonia (PCP). The results show that liver cirrhosis can be an independent risk factor for PCP, especially in patients with severe alcoholic hepatitis who are on corticosteroid treatment for decompensated cirrhosis. Primary prophylaxis for PCP should be considered in these patients.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Review
Microbiology
Kazuto Takeuchi, Yoshihiro Yakushijin
Summary: Pneumocystis jirovecii pneumonia (PJP) is a life-threatening pneumonia commonly seen in immunocompromised patients, and could have significant impacts on cancer patients undergoing chemotherapy, highlighting the importance of PJP prophylaxis.
Article
Dermatology
Margot Salse, Victor Mercier, Marie-Jose Carles, Catherine Lechiche, Milene Sasso
Summary: The study evaluated the performance of the RealStar(R) P jirovecii PCR kit for PCP diagnosis, showing excellent concordance with an in-house PCR. The kit performed well in samples from patients with proven and probable PCP, but had a lower percentage of positive samples in the possible PCP category.
Article
Infectious Diseases
Eun-Ki Min, Juhan Lee, Su Jin Jeong, Deok-Gie Kim, Seung Hyuk Yim, Mun Chae Choi, Dong Jin Joo, Myoung Soo Kim, Jae Geun Lee
Summary: The risk factors for late-onset Pneumocystis jirovecii pneumonia (PCP) after liver transplantation (LT) have been analyzed in this study. Age >= 65 years at LT, cytomegalovirus infection, steroid pulse therapy, hepatocellular carcinoma recurrence, and lymphocytopenia were identified as independent risk factors for PCP. Targeted prophylaxis considering these risk factors could improve the prevention of this potentially lethal complication.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Amjad Kanj, Bilal Samhouri, Nadine Abdallah, Omar Chehab, Misbah Baqir
Summary: The study examined host factors in PCP-related hospitalizations, finding that malignancy was the most prevalent factor, followed by HIV. The prevalence of HIV decreased over time, while non-HIV patients showed higher rates of interventions and worse outcomes compared to HIV patients.
MAYO CLINIC PROCEEDINGS
(2021)
Article
Infectious Diseases
Qin Lang, Lijuan Li, Yue Zhang, Xing He, Yafeng Liu, Zhen Liu, Haiying Yan
Summary: The purpose of this study was to develop and validate a nomogram for the diagnosis of pneumocystis jirovecii pneumonia (PJP) in non-HIV-infected pneumonia patients receiving oral glucocorticoid treatment. The nomogram showed good diagnostic power and could help reduce the mortality rate by promoting timely treatment of PJP.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Gastroenterology & Hepatology
S. Vieujean, A. Moens, D. Hassid, K. Rothfuss, E. Savarino, S. R. Vavricka, C. Reenaers, B. Jacobsen, M. Allez, M. Ferrante, J. F. Rahier
Summary: This study aimed to describe the exposure to immunosuppressive treatment and outcomes in inflammatory bowel disease (IBD) patients with Pneumocystis jirovecii pneumonia (PJP). The results showed that among 17 IBD patients, 18 episodes of PJP were reported, with two deaths. The study highlights the importance of adjusted prophylactic therapy in immunosuppressed IBD patients.
JOURNAL OF CROHNS & COLITIS
(2022)
Article
Immunology
Romain Lecuyer, Nahema Issa, Benoit Tessoulin, Rose-Anne Lavergne, Florent Morio, Frederic Gabriel, Emmanuel Canet, Celine Bressollette-Bodin, Aurelie Guillouzouic, David Boutoille, Francois Raffi, Raphael Lecomte, Paul Le Turnier, Colin Deschanvres, Fabrice Camou, Benjamin Jean Gaborit
Summary: Respiratory cytomegalovirus (CMV) coinfection at diagnosis of Pneumocystis jirovecii pneumonia (PcP) is independently associated with increased 90-day mortality, specifically in HIV-negative patients. This suggests that CMV coinfection may have an adverse impact on the prognosis of PcP patients.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Chemistry, Medicinal
Florian Leidner, Nese Kurt Yilmaz, Celia A. Schiffer
Summary: Using a combination of homology modeling, molecular dynamics, and machine learning, the study identified six molecular features involving mainly residues that did not vary as the best indicators of resistance to trimethoprim in Pneumocystis jirovecii dihydrofolate reductase variants.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Dermatology
Bertrand Nyuykonge, Emmanuel Siddig, Najwa A. Mhmoud, Sahar Bakhiet, Eduard Zijlstra, Annelies Verbon, Ahmed H. Fahal, Wendy W. J. van de Sande
Summary: This study compared the performance of Wako and Fungitell beta-D-glucan assays in patients with eumycetoma. The results showed that when using the optimized cut-off value, the Wako assay and Fungitell assay had comparable performance in terms of sensitivity and specificity. Therefore, the Wako assay can be used in combination with current diagnostic tools.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Dermatology
Emmanuel Edwar Siddig, Bertrand Nyuykonge, Najwa Adam Mhmoud, Omnia Babekir Abdallah, Mustafa El Nour Bahar, Eiman Siddig Ahmed, Borna Nyaoke, Eduard E. Zijlstra, Annelies Verbon, Sahar Mubarak Bakhiet, Ahmed Hassan Fahal, Wendy W. J. van de Sande
Summary: This study compared the diagnostic performance of ultrasound, histopathology, culture, and two species-specific PCRs in identifying the causative organism of mycetoma. The results showed that the two PCR methods were the most sensitive and specific, while ultrasound was the least specific. The evaluation concluded that PCR directly on DNA isolated from grains is the most rapid and reliable diagnostic tool.
Article
Dermatology
Emmanuel Edwar Siddig, Ayman Ahmed, Omnia Babekir Hassan, Sahar Mubarak Bakhiet, Annelies Verbon, Ahmed Hassan Fahal, Wendy W. J. van de Sande
Summary: PCR performed on ultrasound-guided fine-needle aspiration (US-FNA) material has similar sensitivity and specificity as PCR performed on deep-seated biopsies.
Article
Infectious Diseases
Rebecca van Grootveld, Valentina Masarotto, Peter A. von dem Borne, Nicole M. A. Blijlevens, Dana A. Chitu, Martha T. van der Beek, Marta Fiocco, Mark G. J. de Boer
Summary: The study aimed to estimate the cumulative incidence of death due to different causes of death (CODs) in older patients with AML or high-risk MDS, and investigate the effect of IA on each separate COD. The results showed that infection was the most common cause of death in the first year after diagnosis, while leukemia became the most likely cause of death over time. Patients with IA had an increased risk of dying from leukemia or infection.
BMC INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Jingyi Ma, Matthew Todd, Wendy W. J. van de Sande, Bernhard Biersack
Summary: In this study, a series of natural naphthoquinones and anthraquinones were evaluated for their activity against the most common causative agent of eumycetoma. Naphthazarin was identified as a considerably active antifungal compound against Madurella mycetomatis, with reduced toxicity to Galleria mellonella larvae.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Chemistry, Medicinal
Tariq A. Khan, Ibrahim S. Al Nasr, Waleed S. Koko, Jingyi Ma, Simon Eckert, Lucas Brehm, Ridha Ben Said, Ismail Daoud, Riadh Hanachi, Seyfeddine Rahali, Wendy W. J. van de Sande, Klaus Ersfeld, Rainer Schobert, Bernhard Biersack
Summary: Synthetic derivatives of the natural alkaloid piperlongumine, N-acylpyrrolidones and -piperidones, were prepared and tested against Leishmania major and Toxoplasma gondii parasites. Replacement of an aryl meta-methoxy group with halogens increased antiparasitic activities. Some compounds showed strong activity against L. major promastigotes and high activity against T. gondii parasites. QSAR studies and docking calculations revealed binding differences between 2-pyrrolidone and 2-piperidone derivatives. Compound 4 b exhibited microtubules-destabilizing effects in T. b. brucei cells.
Article
Infectious Diseases
Martijn Sijbom, Frederike L. Buchner, Nicholas H. Saadah, Mattijs E. Numans, Mark G. J. De Boer
Summary: This study investigated trends in antibiotic prescribing in primary care and examined the associations between antibiotic selection pressure (ASP) and the prevalence of drug-resistant microorganisms. The findings revealed significant associations between the volume of antibiotic prescribing, ASP, and the prevalence of drug-resistant microorganisms. Countries with higher antibiotic volumes and ASP had higher prevalence of drug-resistant microorganisms. The study suggests that the impact of ASP generated from primary care on increasing antimicrobial resistance may be larger than currently assumed.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Review
Pharmacology & Pharmacy
David J. Chandler, Alexandro Bonifaz, Wendy W. J. van de Sande
Summary: Eumycetoma, a chronic subcutaneous mycosis, has limited treatment options and often requires extensive surgery. This article discusses the need for better treatments, current developments, and future challenges.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Infectious Diseases
Henk Scheper, Rachid Mahdad, Brenda Elzer, Claudia Lowik, Wierd Zijlstra, Taco Gosens, Joris C. T. van der Lugt, Robert J. P. van der Wal, Rudolf W. Poolman, Matthijs P. Somford, Paul C. Jutte, Pieter K. Bos, Richard E. Zwaan, Rob G. H. H. Nelissen, Leo G. Visser, Mark G. J. de Boer
Summary: Moderate to heavy wound drainage and persistent wound drainage were strongly associated with prosthetic joint infection (PJI), and additional parameters are needed to guide the decision to reoperate on patients for suspected acute PJI.
JOURNAL OF BONE AND JOINT INFECTION
(2023)
Letter
Infectious Diseases
Chiara van der Worp, Tara Middeldorp, Laura Kuijpers, Jonna Bank, Lisa Dol, Martha van der Beek, Esther J. van Zuuren, Bart Hendriks, Leo Visser, Mark de Boer, Merel Lambregts
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Roos Wijnakker, Maurits S. van Maaren, Lonneke G. M. Bode, Maja Bulatovic, Bart J. C. Hendriks, Masja C. M. Loogman, Suzanne P. M. Lutgens, Ananja Middel, Chris M. G. Nieuwhof, Eveline E. Roelofsen, Jan W. Schoones, Kim C. E. Sigaloff, Aline B. Sprikkelman, Lieke M. M. de Vrankrijker, Mark G. J. de Boer
Summary: The Dutch Working Party on Antibiotic Policy (SWAB) established a multidisciplinary expert committee to provide evidence-based recommendations for antibiotic therapy in patients with reported antibiotic allergies. A total of 60 recommendations were generated for suspected allergy to β-lactam antibiotics (BLAs) and non-β-lactam antibiotics, based on systematic literature searches and evaluations.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Jingyi Ma, Mickey Konings, Annelies Verbon, Wendy W. J. van de Sande
Summary: Eumycetoma is a common subcutaneous implantation mycosis with the formation of black or white grains. In this study, a grain model of F. senegalensis was established in G. mellonella larvae, and the efficacy of antifungal therapy was evaluated. The results showed that amphotericin B can prolong larval survival. This model can be used to monitor grain formation and study the effectiveness of antifungal drugs.
Article
Dermatology
Bertrand Nyuykonge, Emmanuel E. Siddig, Borna A. Nyaoke, Eduard E. Zijlstra, Annelies Verbon, Sahar M. Bakhiet, Ahmed H. Fahal, Wendy W. J. van de Sande
Summary: The study measured and evaluated (1,3)-beta-D-glucan concentrations in 104 patients with eumycetoma, showing stability before treatment, significant drop after surgery, and low predictive accuracy for recurrence, suggesting limited value in determining treatment response to azoles.
Review
Microbiology
Emmanuel Edwar Siddig, Ayman Ahmed, Hala Fathi Eltigani, Sahar Mubarak Bakhiet, Wendy W. J. van de Sande, Ahmed Hassan Fahal
Summary: This is a case report on a fungal infection caused by Fusarium falciforme, resulting in a recurrent eumycetoma. This is the first report of Fusarium falciforme causing eumycetoma in Sudan, highlighting the need for increased awareness among healthcare providers and improved diagnostic and surveillance systems in at-risk areas to improve case management and reduce the threat of further spread.
Review
Surgery
Laura M. Gerritsen, Henk Scheper, Mark G. J. de Boer, Jan W. Schoones, Rob G. H. H. Nelissen, Bart G. C. Pijls
Summary: This systematic review and meta-analysis evaluated the outcome of DAIR in treating streptococcal hip and knee prosthetic joint infection (PJI). The study included 25 observational studies with a total of 1367 patients. The overall success rate of streptococcal PJI treated with DAIR was 71%, with knee PJI showing a higher success rate (76%) compared to hip PJI (58%).
SURGERY IN PRACTICE AND SCIENCE
(2023)
