Article
Engineering, Environmental
Ke Zhang, Joseph Hodge, Anamika Chatterjee, Tae Seok Moon, Kimberly M. Parker
Summary: Double-stranded RNA undergoes alkaline hydrolysis at orders-of-magnitude slower rates compared to single-stranded RNA, and can remain intact for months at neutral pH. Increasing pH effectively attenuates enzymatic degradation of dsRNA without inducing alkaline hydrolysis, which differs significantly from ssRNA. Understanding these unique properties of dsRNA allows for greater control of stability in RNAi technology and provides insights into various applications, including isolation, detection, and inactivation of dsRNA viruses.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Genetics & Heredity
Ilke Karayel-Hacioglu, Buket Gul, Feray Alkan
Summary: This study reports the presence of PBVs in sheep in Turkey for the first time and provides molecular characterization and epidemiological analysis of PBVs.
Article
Agronomy
Ningbo Chen, Xin Dai, Qian Hu, Hongxiao Tan, Liping Qiao, Laifeng Lu
Summary: Postharvest quality deterioration is a major issue in food loss and waste, but the technique of spray-induced gene silencing (SIGS) offers a new solution. By spraying double-stranded RNA (dsRNA) onto the surface of plants, specific genes can be targeted for RNA interference, effectively reducing browning in freshly-cut potatoes. This study highlights the potential application of SIGS in controlling postharvest quality deterioration.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Steven R. Strezsak, Penny J. Beuning, Nicholas J. Skizim
Summary: A method for accurately determining the concentration of long dsRNA was developed using nucleic acid digestion and reversed-phase HPLC/UV methods. The study found that sequence design can dramatically change the extinction coefficient of the molecule, with ssRNA overhangs leading to a 5% reduction in the calculated extinction coefficient.
ANALYTICAL METHODS
(2021)
Article
Biochemistry & Molecular Biology
Wenbo Xu, Miao Zhang, Yangcun Li, Wanwan He, Shengchun Li, Jiang Zhang
Summary: RNA interference (RNAi) is an effective technology for pest management. This study demonstrates that RNAi can be used to control the 28-spotted potato ladybird by targeting the beta-Actin gene. Transgenic potato plants expressing the target gene showed enhanced resistance to the pest. These findings expand the application range of RNAi technology to Coccinellidae pests.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Microbiology
Alexander Stevens, Katherine Muratore, Yanxiang Cui, Patricia J. Johnson, Z. Hong Zhou
Summary: The atomic structure of TVV2 reveals the interactions driving capsid assembly and the properties governing viral genome organization. Comparison with other dsRNA viruses suggests conserved strategies of RNA release and potential domains involved in viral RNA maintenance. This provides insights for future investigations into the interactions between Trichomonas vaginalis and its viruses.
Article
Plant Sciences
Jinqiu Fu, Shijing Xu, Huan Lu, Fanchi Li, Shengchun Li, Ling Chang, David G. Heckel, Ralph Bock, Jiang Zhang
Summary: Plant-mediated RNA interference (RNAi) using dsRNA or siRNA has shown promise in pest control, but little is known about the molecular mechanisms. This study found that siRNAs derived from transgenic plants may be more effective triggers of RNAi in Lepidoptera than dsRNAs.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Hitomi Sano, Anton Kratz, Taiko Nishino, Haruna Imamura, Yuki Yoshida, Noriaki Shimizu, Hiroaki Kitano, Ayako Yachie
Summary: Based on transcriptome analysis, this study demonstrates that NMN can alleviate the poly(I:C)-induced inflammatory response in human primary cells, HPMECs and HCAECs, by downregulating major inflammatory mediators. Furthermore, network analysis reveals potential common and distinct genes that regulate the identified inflammation modules. These findings suggest a robust regulatory mechanism by which NMN can alleviate inflammatory pathway activation, potentially contributing to the development of new treatments for chronic or acute inflammation.
SCIENTIFIC REPORTS
(2023)
Article
Virology
Jose L. Huaman, Carlo Pacioni, Subir Sarker, Mark Doyle, David M. Forsyth, Anthony Pople, Jordan O. Hampton, Teresa G. Carvalho, Karla J. Helbig
Summary: PBVs were detected in wild deer and cattle in southeastern Australia, with both genogroups I and II being found. The study revealed the predominance of genogroup I and the simultaneous detection of both genogroups in these animals, as well as provided evidence of the respiratory tract tropism of PBVs.
Article
Agronomy
Danielle Duanis-Assaf, Ilya Shlar, Ortal Galsurker, Olga Davydov, Dalia Maurer, Oleg Feygenberg, Elena Poverenov, Robert Fluhr, Noam Alkan
Summary: By incorporating dsRNA into LDH nano-clays, the stability of dsRNA is improved and it can serve as a long-term controlled-release mechanism to selectively control postharvest pathogen B. cinerea. The LDH-dsRNA complex shows continued efficacy after long-term storage and reduces gray mold development significantly.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Endocrinology & Metabolism
Bong Jung Kang, Zakea Sultana, Marcy N. Wilder
Summary: Vitellogenesis-inhibiting hormone (VIH) negatively regulates reproduction in shrimp, and transcriptional silencing of Liv-SGP-G was successfully achieved with dsRNA injections. Although the treatment did not promote ovarian maturation, it was the first report of co-transcriptional repression of Liv-SGP-G using dsRNAs for homologous genes. This study indicates that subadult shrimp can respond to transcriptional silencing techniques.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ah-Young Hong, Seok-Jin Lee, Ki Baek Lee, Ji-Woong Shin, Eui Man Jeong, In-Gyu Kim
Summary: UV-irradiation induces the secretion of dsRNA from damaged noncoding RNAs, which enhances the expression of MMP in keratinocytes, leading to dysregulation of extracellular matrix homeostasis. This study demonstrates that TG2 mediates dsRNA-induced MMP-1 expression through NF-kappa B activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Danielle Duanis-Assaf, Ortal Galsurker, Olga Davydov, Dalia Maurer, Oleg Feygenberg, Moshe Sagi, Elena Poverenov, Robert Fluhr, Noam Alkan
Summary: Pathogenic fungi cause postharvest losses, prompting the need for new control strategies. The development of dsRNA-ERG effectively inhibits fungal growth and grey-mould development, reducing the required fungicide amount.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Bailing Ren, Jingnan Cao, Yanqiu He, Sheng Yang, Jiang Zhang
Summary: RNA interference is a efficient technology for pest control, and expression of dsRNAs in transplastomic plants may have potential effects on non-target pests. The study showed that ACT plants had cross-resistance to another coleopteran insect with highly conserved nucleotide sequence, providing an opportunity for simultaneous control of different pests.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Virology
Mi-Sun Yoo, A-Tai Truong, Hana Jeong, Do-Hyun Hahn, Ju-Seong Lee, Soon-Seek Yoon, So-Youn Youn, Yun-Sang Cho
Summary: Sacbrood virus (SBV) infection poses a significant threat to Apis cerana colonies in South Korea. RNA interference (RNAi) targeting the VP3 gene is an effective method for protecting and treating SBV in vitro and in infected colonies. Laboratory-based experiments and large-scale field trials demonstrate the safety and efficacy of VP3 double-stranded RNA (dsRNA) treatment in increasing survival rates and preventing symptomatic SBV infections.
