Article
Agriculture, Dairy & Animal Science
Mohsen Hanifeh, Thomas Spillmann, Mirja Huhtinen, Yannes S. Sclivagnotis, Thomas Gronthal, Ulla Hynoenen
Summary: The study demonstrates that canine-derived Enterococcus faecalis and E. faecium strains adhere better to the duodenal mucosa of healthy beagles compared to chicken-derived strains, with canine E. faecalis showing the highest hydrophobicity levels. This suggests that both bacterial species and host origin may influence mucosal adhesion.
Article
Microbiology
Renata Kwit, Magdalena Zajac, Aleksandra Smialowska-Weglinska, Magdalena Skarzynska, Arkadiusz Bomba, Anna Lalak, Ewelina Skrzypiec, Dominika Wojdat, Weronika Koza, Emilia Mikos-Wojewoda, Paulina Pasim, Milena Skora, Marcin Polak, Jaroslaw Wiacek, Dariusz Wasyl
Summary: This study aimed to assess the prevalence of Enterococcus spp. in wild birds in Poland, determine their antimicrobial susceptibility, and conduct Whole Genome Sequencing (WGS) analysis. The results showed that out of 138 samples from various species of free-living birds, 66.7% tested positive for Enterococcus. Fourteen species were detected, with Enterococcus faecalis being the most common. Resistance to antimicrobial agents was observed in some E. faecalis and E. faecium strains, along with the presence of multidrug resistance phenotype in one E. faecium strain. WGS analysis also revealed variations in virulence gene diversity between E. faecalis and E. faecium strains, as well as the presence of plasmid replicons in both strains.
Article
Infectious Diseases
Sonia Nina Coccitto, Marzia Cinthi, Simona Fioriti, Gianluca Morroni, Serena Simoni, Carla Vignaroli, Cristiana Garofalo, Marina Mingoia, Andrea Brenciani, Eleonora Giovanetti
Summary: This study characterized a linezolid-resistant Enterococcus gallinarum isolate from pigs that carried the cfr, optrA, and poxtA genes. Two plasmids were identified, each carrying different resistance genes. The plasmids showed features of circular intermediates and were not transferable to other enterococcal strains. The persistence of these plasmids in the absence of selective pressure is concerning.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Laura Escola-Verge, Nuria Fernandez-Hidalgo, Maria Nieves Larrosa, Ruben Fernandez-Galera, Benito Almirante
Summary: The study showed an increasing prevalence of E. faecalis as a causative agent of infective endocarditis (EFIE) over the past decade, along with clinical changes including older patient age, fewer surgical indications, and a slightly decreased in-hospital mortality rate. Additionally, there was an increase in the percentage of echocardiograms performed in patients with EFMB and EFIE diagnoses. Further investigations into the factors driving these clinical changes in EFIE are warranted.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Hiroshi Sasano, Kazuhiko Hanada
Summary: This study aimed to investigate the clinical outcomes and evaluate the risk factors for mortality in adult patients treated with vancomycin for vancomycin-susceptible Enterococcus faecium bacteremia. The results showed that concurrent use of vasopressors may be an independent risk factor for mortality.
Article
Food Science & Technology
Marlena Golas-Pradzynska, Magdalena Luszczynska, Jolanta Grazyna Rola
Summary: This study investigates the antimicrobial resistance, virulence, and resistance genes of Enterococcus faecalis and Enterococcus faecium isolated from raw goat's and sheep's milk and cheese. The results show that E. faecalis is the dominant species, with high levels of antimicrobial resistance and presence of virulence genes in some strains.
Article
Infectious Diseases
Lucia Malisova, Vladislav Jakubu, Katarina Pomorska, Martin Musilek, Helena Zemlickova
Summary: The main mechanisms of linezolid resistance in linezolid-resistant enterococci in the Czech Republic were found to be the G2576T mutation in the domain V of 23S rRNA and the presence of the optrA gene, with some isolates also showing resistance to tigecycline and vancomycin.
Review
Medicine, General & Internal
Masoud Dadashi, Parastoo Sharifian, Nazila Bostanshirin, Bahareh Hajikhani, Narjess Bostanghadiri, Nafiseh Khosravi-Dehaghi, Alex van Belkum, Davood Darban-Sarokhalil
Summary: Based on the analysis of 114 studies worldwide, it was found that the prevalence of antibiotic-resistant E. faecalis and E. faecium to linezolid, tigecycline, and daptomycin is relatively low but increasing.
FRONTIERS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Elham Boodaghi Malidareh, Mohammad Ahanjan, Saba Asgharzadeh Marghmalek, Hamid Reza Goli
Summary: The high resistance rates and prevalence of resistance genes in enterococci isolated from the stool of healthy individuals and the hospital environment highlight the potential transmission of resistance genes to clinical isolates.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Infectious Diseases
Grace Mwikuma, Henson Kainga, Simegnew Adugna Kallu, Chie Nakajima, Yasuhiko Suzuki, Bernard Mudenda Hang'ombe
Summary: The study aimed to determine the prevalence and patterns of antimicrobial resistance and detect drug-resistant genes in Enterococcus species in poultry from four districts in Zambia. The results showed that poultry are a potential source of multidrug-resistant Enterococcus strains, which can be transmitted to humans, posing a significant public health concern.
Article
Infectious Diseases
Mary Catherine McEllistrem, Hayley R. Nordstrom, Aaron Lucas, Brooke K. Decker, Daria Van Tyne
Summary: VRE bacteremia is associated with higher mortality rates and longer hospitalizations compared to VSE bacteremia. Differences in genetic characteristics and biofilm formation exist between VSE and VRE strains, with VRE strains potentially remaining protected in the heart valves and causing infection when disrupted during cardiac surgery. This study highlights the importance of understanding bacterial adherence and biofilm formation in endocarditis patients.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Andreas Berge, Ludvig Arkel, Bo Nilson, Magnus Rasmussen
Summary: The management and outcome of patients with cardiac implantable electronic devices (CIED) and monomicrobial Enterococcus faecalis bacteremia (EfsB) were studied. Extraction of the CIED was found to be unnecessary if no structural changes were found on the CIED.
Article
Infectious Diseases
Athraa Harjan Mohsen Alduhaidhawi, Sundus Nsaif AlHuchaimi, Thikra Abdullah Al-Mayah, Mushtak T. S. Al-Ouqaili, Samar Sami Alkafaas, Saravanan Muthupandian, Morteza Saki
Summary: This study revealed a higher prevalence of E. faecalis than E. faecium in hospital wastewater (HWW). The lack of CRISPR-Cas genes was associated with higher antibiotic resistance rates and multidrug resistance in E. faecalis and E. faecium isolates from HWW.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Beatriz Rizzo Paschoalini, Karen Vanessa Munive Nunez, Juliana Takahashi Maffei, Helio Langoni, Felipe Freitas Guimaraes, Clarice Gebara, Natylane Eufransino Freitas, Marcos Veiga dos Santos, Carlos Eduardo Fidelis, Roberto Kappes, Monica Correia Goncalves, Nathalia Cristina Cirone Silva
Summary: This study evaluates the antimicrobial resistance and virulence characteristics of Enterococcus spp. isolated from bovine milk, showing high resistance in E. faecium and E. faecalis. The findings highlight the potential risks and the need for control strategies in dairy farms.
Article
Microbiology
Yanpeng Xiong, Junwen Chen, Xiang Sun, Guangjian Xu, Peiyu Li, Qiwen Deng, Zhijian Yu, Zhong Chen, Jinxin Zheng
Summary: Telithromycin demonstrated potent in vitro antibacterial activity against clinical enterococci isolates in China, especially strains carrying erythromycin-resistant methylase genes. Subinhibitory concentrations of telithromycin inhibited biofilm formation of Enterococcus strains, and the combination of telithromycin and ampicillin showed enhanced antibiofilm activity against established biofilms. These findings suggest telithromycin's potential as a candidate for enterococcal infection treatment.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jung-Yien Chien, Chong-Jen Yu, Po-Ren Hsueh
Summary: The performance of metagenomic next-generation sequencing (mNGS) was evaluated and compared with that of conventional culture testing in patients with sepsis. mNGS had higher detection rates than blood culture and BALF culture, especially for bacteria, viruses, and multipathogen infections. RNA-based mNGS increased the detection rate of several bacteria, fungi, and viruses. mNGS offers a sensitive diagnostic method for patients with sepsis, but clinical correlation is advised to interpret the data due to the lack of unified diagnostic criteria.
MICROBIOLOGY SPECTRUM
(2022)
Letter
Infectious Diseases
Jia-Arng Lee, Shin-Hei Du, Tai-fen Lee, Yu-Shan Huang, Chun-Hsing Liao, Yu-Tsung Huang, Po-Ren Hsueh
JOURNAL OF INFECTION
(2023)
Letter
Infectious Diseases
Jia-Arng Lee, Tai-Fen Lee, Yu-Tsung Huang, Po-Ren Hsueh
JOURNAL OF INFECTION
(2022)
Letter
Infectious Diseases
Yuag-Meng Liu, Wen-Chien Ko, Mao-Wang Ho, Yu-Lin Lee, Po-Ren Hsueh
JOURNAL OF INFECTION
(2023)
Letter
Infectious Diseases
Jiaxin Yu, Hsiu-Hsien Lin, Kun-Hao Tseng, Ni Tien, Po-Ren Hsueh, Der-Yang Cho
JOURNAL OF INFECTION
(2023)
Review
Virology
Chih-Cheng Lai, Chi-Kuei Hsu, Shun-Chung Hsueh, Muh-Yong Yen, Wen-Chien Ko, Po-Ren Hsueh
Summary: Following the emergence of multisystem inflammatory syndrome in children (MIS-C), a similar clinical scenario called multisystem inflammatory syndrome in adults (MIS-A) has been observed. Although the prevalence of MIS-A is low and likely underdiagnosed, it can be associated with high mortality. MIS-A is a multisystem disease that affects the cardiovascular, respiratory, gastrointestinal, dermatologic, hematologic, and neurologic systems. The diagnosis of MIS-A requires clinical manifestations, laboratory evidence of inflammation, and SARS-CoV-2 infection. The appropriate treatment for MIS-A is still uncertain, but anti-inflammatory agents like intravenous immunoglobulin and corticosteroids are commonly used. Further research is needed to determine the true prevalence, pathogenesis, and effective treatment for MIS-A.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Review
Virology
Chih-Cheng Lai, Po-Ren Hsueh
Summary: Nirmatrelvir/ritonavir (NMV-r) is an effective antiviral agent for treating nonhospitalized COVID-19 patients. However, there have been rare cases of virologic and symptomatic rebound after NMV-r treatment, which is a cause for concern. This review provides comprehensive information on the frequency, mechanism, outcomes, and management of COVID-19 rebound after NMV-r. The incidence of rebound was less than 2% and most cases had mild symptoms, with favorable clinical outcomes.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Hon-Yen Wu, Yi-Tsung Lin, Wan-Chuan Tsai, Yen-Ling Chiu, Mei-Ju Ko, Ju-Yeh Yang, Mei-Fen Pai, Hsin-Sui Hsu, Shih-Ping Hsu, Yu-Sen Peng, Chun-Hsing Liao
Summary: This study aimed to investigate the distribution of Enterobacteriaceae in the gut microbiota of hemodialysis patients and analyze its association with clinical parameters. The study found significant differences in the gut microbiota composition at the genus level between hemodialysis patients and the control group, with an increase in the Escherichia-Shigella complex and an association between Klebsiella and emergency room visits. Therefore, further research on the microbiota composition of Enterobacteriaceae is necessary.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Chun-Hsing Liao, Ren-Hsuan Ku, Li-Hua Li, Cheng-Mu Wu, Tsuey-Ching Yang
Summary: In our recent study, we found that the yceA-cybB-yceB gene cluster is up-regulated in the presence of oxidative stress. This gene cluster plays a role in alleviating oxidative stress, enhancing swimming motility, and increasing susceptibility to certain antibiotics.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Letter
Infectious Diseases
Pin-Han Wu, Chih-Hao Chen, Wen-Hsin Hsih, Chia-Huei Chou, Chih-Yu Chi, Mao-Wang Ho, Yu-Tzu Lin, Hsiu-Hsien Lin, Kun-Hao Tseng, Po-Ren Hsueh
JOURNAL OF INFECTION
(2023)
Article
Immunology
I-Ru Chen, Guei-Jane Wang, Po-Ren Hsueh, Chia-Hui Chou, Long-Bin Jeng, Hui-Ju Lin, Huang-Jiun Liao, Ping-Chin Lai, Jan-Gowth Chang, Chiu-Ching Huang
Summary: This study evaluated the immune responses and safety of COVID-19 vaccination in patients with atypical hemolytic uremic syndrome (aHUS) in Taiwan. The results showed that the vaccines effectively protected aHUS patients from severe COVID-19 complications without significant safety concerns. Transplant aHUS patients had lower immune response titers, indicating a need for additional vaccine doses.
Article
Infectious Diseases
Bing-Jie Shen, Jann-Tay Wang, Hou-Tai Chang, Shan-Chwen Chang, Chun-Hsing Liao
Summary: Active surveillance for sputum cultures revealed Burkholderia cepacia infection in an intensive care unit from September 2008 to September 2009. The use of disposable ventilator circuits resulted in a decrease in infection rates, and this effect persisted in the following years.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Medicine, Research & Experimental
Chang Yi Wang, Kao-Pin Hwang, Hui-Kai Kuo, Wen-Jiun Peng, Yea-Huei Shen, Be-Sheng Kuo, Juin-Hua Huang, Hope Liu, Yu-Hsin Ho, Feng Lin, Shuang Ding, Zhi Liu, Huan-Ting Wu, Ching-Tai Huang, Yuarn-Jang Lee, Ming-Che Liu, Yi-Ching Yang, Po-Liang Lu, Hung-Chin Tsai, Chen-Hsiang Lee, Zhi-Yuan Shi, Chun-Eng Liu, Chun-Hsing Liao, Feng-Yee Chang, Hsiang-Cheng Chen, Fu-Der Wang, Kuo-Liang Hou, Jennifer Cheng, Min-Sheng Wang, Ya-Ting Yang, Han-Chen Chiu, Ming-Han Jiang, Hao-Yu Shih, Hsuan-Yu Shen, Po-Yen Chang, Yu-Rou Lan, Chi-Tian Chen, Yi-Ling Lin, Jian-Jong Liang, Chun-Che Liao, Yu-Chi Chou, Mary Kate Morris, Carl V. Hanson, Farshad Guirakhoo, Michael Hellerstein, Hui-Jing Yu, Chwan-Chuen King, Tracy Kemp, D. Gray Heppner, Thomas P. Monath
Summary: The UB-612 vaccine showed favorable safety profile, long-lasting antibody response, and broad T cell immunity against SARS-CoV-2 variants, including Delta and Omicron, in phase I/II trials.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Cell Biology
Chun-Hsing Liao, Hsu-Feng Lu, Hsin-Hui Huang, Yu Chen, Li-Hua Li, Yi-Tsung Lin, Tsuey-Ching Yang
Summary: This study focused on elucidating the ferric citrate acquisition system of S. maltophilia, revealing the involvement of FciTABC and FeoABI systems in ferric citrate acquisition.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)