期刊
JOURNAL OF IMMUNOLOGY
卷 193, 期 5, 页码 2218-2229出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1203441
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资金
- National Institute of Allergy and Infectious Diseases [1R56AI085474]
- National Center for Advancing Translational Sciences [KL2TR000072, TR000071]
- American Gastroenterology Association
- American Cancer Society [RSG-10-159-01-LIB]
- Clinical and Translational Science Award Multi-Institutional Pilot Fund
- Crohn's & Colitis Foundation of America
Signaling via programmed death ligand-1 (PD-L1) and PD-L2 is crucial for maintaining peripheral tolerance. CD90(+) myofibroblasts/fibroblasts (CMFs) are major programmed cell death-1 (PD-1) ligand-expressing cells in normal human colonic mucosa. CMFs suppress activated CD4(+) T cell proliferation via PD-1 ligands. It is not known whether signaling through TLRs contribute to the regulation PD-1 ligands on CMFs upon colonic mucosal tolerance. In this study, we demonstrated that stimulation of TLR4 on human CMFs upregulates PD-L1, but not PD-L2, and reinforces CMF-mediated suppression of CD4(+) T cell proliferation and IFN-gamma production. TLR4-mediated upregulation of PD-L1 on CMFs involved NF-kappa B pathways and was JAK2 and MyD88 dependent. MyD88-dependent stimulation of TLR1/2 and TLR5 also upregulated PD-L1 expression on CMFs in culture. PD-L1 expression was drastically decreased in vivo in the colonic mucosa of mice devoid of MyD88. Induction of MyD88 deficiency in CMFs in fibroblast-specific MyD88 conditional knockout mice resulted in a strong increase in a mucosal IFN-gamma expression concomitantly with the abrogation of PD-L1 expression in CMFs under homeostasis and epithelial injury induced by dextran sodium sulfate. Together, these data suggest that MyD88-dependent TLR stimulation of CMFs in the normal colonic mucosa may reinforce these cells' anti-inflammatory capacity and thus contribute to the maintenance of mucosal tolerance.
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