期刊
JOURNAL OF IMMUNOLOGY
卷 193, 期 4, 页码 1622-1635出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1401243
关键词
-
类别
资金
- I2HD CIML-SANOFI project
- INSERM
- Agence Nationale de la Recherche (EMICIF) [ANR-08-MIEN-008-02]
- Agence Nationale de Recherches sur le SIDA et les Hepatites Virales
- Institut National du Cancer [2011-155]
- Ligue contre la Cancer (Comite de la Savoie)
- Lyon Biopole (France)
- Association pour la Recherche sur le Cancer (France)
- Fondation pour la Recherche Medicale
- European Research Council under the European Community [281225]
- Agence Nationale de la Recherche (PhyloGenDC) [ANR-09-BLAN-0073-02]
- European Research Council [281225]
- European Research Council (ERC) [281225] Funding Source: European Research Council (ERC)
- Agence Nationale de la Recherche (ANR) [ANR-09-BLAN-0073] Funding Source: Agence Nationale de la Recherche (ANR)
Human monocyte-derived dendritic cell (MoDC) have been used in the clinic with moderately encouraging results. Mouse XCR1(+) DC excel at cross-presentation, can be targeted in vivo to induce protective immunity, and share characteristics with XCR1(+) human DC. Assessment of the immunoactivation potential of XCR1(+) human DC is hindered by their paucity in vivo and by their lack of a well-defined in vitro counterpart. We report in this study a protocol generating both XCR1(+) and XCR1(-) human DC in CD34(+) progenitor cultures (CD34-DC). Gene expression profiling, phenotypic characterization, and functional studies demonstrated that XCR1(-) CD34-DC are similar to canonical MoDC, whereas XCR1(+) CD34-DC resemble XCR1(+) blood DC (bDC). XCR1(+) DC were strongly activated by polyinosinic-polycytidylic acid but not LPS, and conversely for MoDC. XCR1(+) DC and MoDC expressed strikingly different patterns of molecules involved in inflammation and in cross-talk with NK or T cells. XCR1(+) CD34-DC but not MoDC efficiently cross-presented a cell-associated Ag upon stimulation by polyinosinic-polycytidylic acid or R848, likewise to what was reported for XCR1(+) bDC. Hence, it is feasible to generate high numbers of bona fide XCR1(+) human DC in vitro as a model to decipher the functions of XCR1(+) bDC and as a potential source of XCR1(+) DC for clinical use.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据