Article
Biochemistry & Molecular Biology
Alessandra Pulliero, Luca Mastracci, Letizia Tarantini, Zumama Khalid, Valentina Bollati, Alberto Izzotti
Summary: This study reveals the relationship between KRAS mutation and let-7a downregulation in cigarette smoke-induced lung carcinogenesis. Long-term exposure to cigarette smoke leads to a significant decrease in let-7a expression and an increase in KRAS mutation, resulting in a higher incidence of lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Philip B. Busbee, Marpe Bam, Xiaoming Yang, Osama A. Abdulla, Juhua Zhou, Jay Paul (Jack) Ginsberg, Allison E. Aiello, Monica Uddin, Mitzi Nagarkatti, Prakash S. Nagarkatti
Summary: Post-traumatic stress disorder (PTSD) patients often have comorbid autoimmune and inflammatory disorders. Studies have shown that PTSD patients exhibit increased inflammatory T helper cell phenotype and downregulated miRNAs, which affect the development of Th17 cells by modulating TP53 expression. Additionally, decreased expression of TP53 is associated with an increase in the inflammatory Th17 phenotype in PTSD patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Medicinal
Zhen Song, Qiang-Yan Zhang, Jia-Jing Li, Jing-Lian Su, Yuan-Hua Liu, Gong-Jun Yang, Huai-Song Wang
Summary: Let-7a, a type of low-expressed microRNAs in cancer cells, has been investigated as a promising biomarker and therapeutic target for tumor suppression. Developing simple and sensitive detection methods for let-7a is important for cancer diagnosis and treatment. In this work, the hybridization chain reaction (HCR) was used to initiate the detection of let-7a, followed by analysis using lateral flow assay (LFA) and electrochemical impedance spectroscopy.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Dermatology
Liquan Wang, Tianhao Li, Xuda Ma, Yunzhu Li, Zhujun Li, Ziming Li, Nanze Yu, Jiuzuo Huang, Qin Han, Xiao Long
Summary: This study suggests that ADSC-Exo may be able to prevent the activity, collagen deposition, and myofibroblast trans-differentiation of LSFs. The authors confirm that let-7a-5p in ADSC-Exo can directly target TGF-R1 to control the Smad pathway and reduce fibrosis in LSFs.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2023)
Article
Immunology
Patrick Coit, Kaila L. Schollaert, Emily M. Mirizio, Kathryn S. Torok, Amr H. Sawalha
Summary: The study assessed DNA methylation differences between juvenile systemic sclerosis (jSSc) and juvenile localized scleroderma (jLS), revealing distinct epigenetic patterns and unique methylated genes in both diseases. Compared to healthy controls, differential methylation sites and genes were identified in jSSc and jLS, indicating potential novel diagnostic biomarkers for localized scleroderma. Pathway analysis showed enrichment of inflammatory pathways in jSSc and the HIPPO signaling pathway in jLS, highlighting different biological processes involved in each condition.
CLINICAL IMMUNOLOGY
(2021)
Article
Chemistry, Analytical
Cuiping Fu, Xuefeng Wang, Fumin Xue, Peihua Zhu, Weijia Zhou, Shenguang Ge, Jinghua Yu
Summary: A photoelectrochemical biosensor based on a multiple signal amplification strategy was developed for highly sensitive detection of microRNA. The sensor utilized TiO2 and CuInS2-sensitized TiO2 layers to enhance photoelectrical properties and achieve initial signal amplification. Signal amplification was further carried out through an enzyme-free hybridization reaction. The sensor exhibited excellent linear response, with a wide detection range and low detection limit. Clinical biomolecular monitoring of miRNA using this sensor demonstrated excellent quantitative detection capability.
Article
Chemistry, Analytical
Lingli Wang, Tengteng Shan, Lu Pu, Jin Zhang, Rong Hu, Yunhui Yang, Jianmei Yang, Yan Zhao
Summary: A sensitive and portable biosensor for simple detection of microRNAs is proposed, based on a supersandwich hybridization signal amplification strategy and a glucometer transducer. The biosensor can accurately quantify microRNAs and discriminate them from their family members, making it suitable for serum sample detection.
Article
Food Science & Technology
Wen-Ting Chen, Meei-Ju Yang, Yi-Wei Tsuei, Tsung-Chen Su, An-Ci Siao, Yow-Chii Kuo, Ling-Ru Huang, Yi Chen, Sy-Jou Chen, Po-Chuan Chen, Ching-Feng Cheng, Hui-Chen Ku, Yung-Hsi Kao
Summary: This study investigates the effect of epigallocatechin gallate (EGCG) on the growth of white and beige preadipocytes by modulating the miR-let-7a and HMGA2 pathways. The results show that EGCG inhibits the growth of 3T3-L1 and D12 preadipocytes, upregulates miR-let-7a expression, and downregulates HMGA2 mRNA and protein levels. Overexpression of miR-let-7a and HMGA2 antagonizes the inhibitory effects of EGCG on cell growth. Therefore, EGCG acts on preadipocytes through the miR-let-7a/HMGA2 pathway to inhibit their growth.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Engineering, Biomedical
Zhiwen Luo, Yaying Sun, Beijie Qi, Jinrong Lin, Yisheng Chen, Yuzhen Xu, Jiwu Chen
Summary: The study found that bone marrow mesenchymal stem cell-derived extracellular vesicles (BMSC-EVs) can significantly inhibit the fibrogenic process in shoulder capsule fibrosis. BMSC-EVs suppressed abnormal proliferation and high collagen production in fibroblasts, improving shoulder mobility. The let-7a-5p molecule in BMSC-EVs was found to have anti-fibrotic effects by targeting TGFBR1 gene.
BIOACTIVE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Chien-Hsien Lo, Li-Ching Li, Shun-Fa Yang, Chin-Feng Tsai, Yao-Tsung Chuang, Hsiao-Ju Chu, Kwo-Chang Ueng
Summary: This study investigated the role of miRNAs in modulating fibrotic molecular mechanisms in response to hypoxic conditions, and found that miR-let-7a, miR-let-7e, and miR-133a play important roles in hypoxia-related atrial fibrosis by inhibiting collagen expression and post-transcriptional repression by the JNK pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Chien-Hsiu Li, Chiao-Chun Liao
Summary: Cancer is often the result of abnormal glucose uptake and imbalanced nutrient metabolism. Dysregulation of glucose metabolism and autophagy, as well as the involvement of miRNA Let-7, play crucial roles in tumor progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
Ying Huang, Manshu Yu, Akihiro Kuma, Janet D. Klein, Yanhua Wang, Faten Hassounah, Hui Cai, Xiaonan H. Wang
Summary: This study found that Acu/LFES enhances protein synthesis in both hindlimb and forelimb muscles by reducing let-7-5p levels, which leads to upregulation of the Igf1 signaling pathway. This provides a new understanding of how electrical acupuncture treatment positively influences muscle health.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Endocrinology & Metabolism
Maryam Daneshvar, Mansoureh Movahedin, Mohammad Salehi, Mehrdad Noruzinia
Summary: The study evaluated the impact of re-vitrification on the expression of miR-16, miR-let7a, and target genes in human blastocysts. The results showed that re-vitrification did not affect the ability of blastocysts to re-expand in culture, but led to a significant decrease in the expression of miR-16 and miR-let7a, along with a significant upregulation of the target genes ITG beta 3 and BCL-2 in re-vitrified and vitrified embryos.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Environmental Sciences
Valeria Longo, Gaspare Drago, Alessandra Longo, Silvia Ruggieri, Mario Sprovieri, Fabio Cibella, Paolo Colombo
Summary: There is growing evidence that environmental pollutants can induce epigenetic modifications altering miRNA expression and leading to pathological conditions in animals. This study found associations between serum concentrations of various inorganic and organic pollutants and the expression of miRNAs in pregnant women. These findings provide innovative early-warning approaches to human health risk assessment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Respiratory System
Donato Lacedonia, Giulia Scioscia, Piera Soccio, Massimo Conese, Lucia Catucci, Grazia P. Palladino, Filomena Simone, Carla M. I. Quarato, Sante Di Gioia, Roberto Rana, Francesco Sollitto, Maria P. Foschino-Barbaro
Summary: This study analyzed the expression of exosomal miRNAs in serum from IPF patients and found significant down-regulation of let-7d and unexpectedly, miR-16. A clustering of miR-16, miR-21, and miR-26a expression was also identified. These findings may help identify key players in the pathophysiology of IPF and potential targets for future medications.
BMC PULMONARY MEDICINE
(2021)
Letter
Dermatology
Tselmeg Mijiddorj Myangat, Shoma Wada, Soichiro Sawamura, Ikko Kajihara, Toshihiro Kimura, Kayo Kashiwada-Nakamura, Katsunari Makino, Jun Aoi, Shinichi Masuguchi, Satoshi Fukushima
JOURNAL OF DERMATOLOGY
(2022)
Letter
Dermatology
Kayo Kashiwada-Nakamura, Tselmeg Mijiddorj Myangat, Ikko Kajihara, Hisashi Kanemaru, Soichiro Sawamura, Katsunari Makino, Jun Aoi, Shinichi Masuguchi, Satoshi Fukushima
JOURNAL OF DERMATOLOGY
(2022)
Letter
Dermatology
Hiromitsu Noguchi, Tadahiko Matsumoto, Utako Kimura, Masataro Hiruma, Rui Kano, Takashi Yaguchi, Kayo Kashiwada-Nakamura, Satoshi Fukushima
JOURNAL OF DERMATOLOGY
(2022)
Article
Dermatology
Atsushi Fukunaga, Satoshi Fukushima, Hiroaki Iwata, Makiko Nakahara, Rikako Sasaki, Naoko Baba, Hiroshi Matsunaka, Yumi Murakami, Masutaka Furue, Chikako Nishigori
Summary: The study investigated the potential of bioactive substances in the stratum corneum as early and sensitive indicators of sun exposure on the skin using ROC analysis. Results showed that IL-1ra/IL-1 alpha, TNF-alpha, and Bax in the stratum corneum are sensitive to sun exposure and decreased significantly after sunscreen application, indicating their potential as markers for sun damage.
PHOTODERMATOLOGY PHOTOIMMUNOLOGY & PHOTOMEDICINE
(2022)
Article
Immunology
Yusuke Miyashita, Takanobu Yoshida, Yuriko Takagi, Hirotake Tsukamoto, Ken Takashima, Takahisa Kouwaki, Katsunari Makino, Satoshi Fukushima, Kimitoshi Nakamura, Hiroyuki Oshiumi
Summary: The study found that EV miR-92a-2-5p levels in serum were negatively correlated with adverse reactions after COVID-19 vaccination, while EV miR-148a levels were associated with specific antibody titers. This suggests that circulating EV miRNAs could serve as biomarkers for vaccine efficacy and adverse reactions.
Review
Oncology
Hisashi Kanemaru, Yukari Mizukami, Akira Kaneko, Ikko Kajihara, Satoshi Fukushima
Summary: Because of the success of novel therapeutic approaches, less invasive and repeatable monitoring tools are needed for melanoma. Liquid biopsy, specifically microRNA, cell-free DNA, and circulating tumor cells obtained from blood samples, has shown promising potential as a diagnostic and prognostic tool for melanoma. Despite challenges, recent technical advances have improved the sensitivity and provided high-throughput analysis for liquid biopsy. Standardizing assay procedures and increasing sensitivity are necessary for widespread clinical implementation, but liquid biopsy could be utilized quickly. This review emphasizes the importance of liquid biopsy and its potential future perspectives.
CURRENT TREATMENT OPTIONS IN ONCOLOGY
(2022)
Letter
Dermatology
Yudo Kusaba, Ikko Kajihara, Tselmeg Mijiddorj Myangat, Kenichiro Tanaka, Hisashi Kanemaru, Soichiro Sawamura, Katsunari Makino, Jun Aoi, Shinichi Masuguchi, Satoshi Fukushima
JOURNAL OF DERMATOLOGY
(2022)
Article
Dermatology
Hiromitsu Noguchi, Tadahiko Matsumoto, Utako Kimura, Masataro Hiruma, Rui Kano, Takashi Yaguchi, Masahide Kubo, Kayo Kashiwada-Nakamura, Satoshi Fukushima
Summary: This study reported two cases of phaeohyphomycosis caused by Exophiala jeanselmei and E. oligosperma. The patients were successfully treated with fosravuconazole and terbinafine, respectively. A summary of 32 cases of cutaneous and subcutaneous phaeohyphomycosis caused by Exophiala species in Japan was included, with different antifungal treatments and their success rates discussed.
JOURNAL OF DERMATOLOGY
(2022)
Letter
Dermatology
Yudo Kusaba, Ikko Kajihara, Soichiro Sawamura, Hisashi Kanemaru, Katsunari Makino, Jun Aoi, Shinichi Masuguchi, Jun Morinaga, Satoshi Fukushima
JOURNAL OF DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Soichiro Sawamura, Katsunari Makino, Maho Ide, Shuichi Shimada, Ikko Kajihara, Takamitsu Makino, Masatoshi Jinnin, Satoshi Fukushima
Summary: In patients with systemic sclerosis (SSc), the levels of alpha 2(I) collagen are higher than alpha 1(I) collagen in fibroblasts. Further investigation of the overall regulatory mechanisms of type I collagen may help to understand the aberrant collagen metabolism in SSc.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Dermatology
Takashi Inozume, Satoshi Fukushima
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Dermatology
Satoshi Fukushima, Azusa Miyashita, Haruka Kuriyama, Toshihiro Kimura, Satoru Mizuhashi, Yosuke Kubo, Satoshi Nakahara, Hisashi Kanemaru, Nobuhiro Tsuchiya, Hiroaki Mashima, Rong Zhang, Yasushi Uemura
Summary: Cancer immunotherapy is the primary treatment for unresectable cancers, but it is not a complete cure for all patients. Immune cell therapy, specifically using immune cells generated from induced pluripotent stem cells (iPSCs), shows promise for innovative cancer immunotherapy methods. iPSC-derived dendritic cells (iPS-DCs) can activate T cells, while iPSC-derived macrophages (iPS-MPs) attack cancer cells. iPSCs offer a source for genetic modification and addition of immune functions. Additionally, immortalized iPS-DCs and iPS-MPs could potentially reduce costs through mass production. This review summarizes the achievements and future prospects of cancer research using iPS-DCs and iPS-MPs.
EXPERIMENTAL DERMATOLOGY
(2023)
Letter
Dermatology
Tomohiro Ishino, Tselmeg Mijiddorj Myangat, Soichiro Sawamura, Ikko Kajihara, Shuichi Shimada, Hisashi Kanemaru, Kayo Kashiwada-Nakamura, Katsunari Makino, Shinichi Masuguchi, Satoshi Fukushima
JOURNAL OF DERMATOLOGY
(2023)
Letter
Dermatology
Chiaki Obaru, Toshihiro Kimura, Manami Yamamura, Haruka Kuriyama, Kayo Kashiwada-Nakamura, Satoru Mizuhashi, Tomoyo Matsumura, Takahiro Watanabe, Toshihiro Inoue, Satoshi Fukushima
JOURNAL OF DERMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Chikako Senju, Yuka Nakazawa, Taichi Oso, Mayuko Shimada, Kana Kato, Michiko Matsuse, Mariko Tsujimoto, Taro Masaki, Yasushi Miyazaki, Satoshi Fukushima, Satoshi Tateishi, Atsushi Utani, Hiroyuki Murota, Katsumi Tanaka, Norisato Mitsutake, Shinichi Moriwaki, Chikako Nishigori, Tomoo Ogi
Summary: Xeroderma pigmentosum (XP) is a rare genodermatosis characterized by photosensitivity and a high risk of skin tumors due to DNA repair deficiency. This study identified two deep intronic mutations in the ERCC4/XPF gene in 17 cases of XP-F, a rare subtype of XP. These mutations result in reduced gene expression and early-onset skin cancers, highlighting the need for attention to these variants. Additionally, antisense oligonucleotides designed for these mutations can restore DNA repair capacity, suggesting potential therapeutic targets for XP-F.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)