期刊
JOURNAL OF IMMUNOLOGY
卷 192, 期 3, 页码 1267-1276出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1301757
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资金
- National Institutes of Health [DK50189, DK095491, DK096709, HL60569]
- Crohn's and Colitis Foundation of America
Cytokines secreted at sites of inflammation impact the onset, progression, and resolution of inflammation. In this article, we investigated potential proresolving mechanisms of IFN-gamma in models of inflammatory bowel disease. Guided by initial microarray analysis, in vitro studies revealed that IFN-gamma selectively induced the expression of IL-10R1 on intestinal epithelia. Further analysis revealed that IL-10R1 was expressed predominantly on the apical membrane of polarized epithelial cells. Receptor activation functionally induced canonical IL-10 target gene expression in epithelia, concomitant with enhanced barrier restitution. Furthermore, knockdown of IL-10R1 in intestinal epithelial cells results in impaired barrier function in vitro. Colonic tissue isolated from murine colitis revealed that levels of IL-10R1 and suppressor of cytokine signaling 3 were increased in the epithelium and coincided with increased tissue IFN-gamma and IL-10 cytokines. In parallel, studies showed that treatment of mice with rIFN-gamma was sufficient to drive expression of IL-10R1 in the colonic epithelium. Studies of dextran sodium sulfate colitis in intestinal epithelial-specific IL-10R1-null mice revealed a remarkable increase in disease susceptibility associated with increased intestinal permeability. Together, these results provide novel insight into the crucial and underappreciated role of epithelial IL-10 signaling in the maintenance and restitution of epithelial barrier and of the temporal regulation of these pathways by IFN-gamma.
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