Review
Immunology
Arianne C. Richard
Summary: The advent of technologies that can characterize individual cells has revealed extensive diversity between cells of the same subset, including CD8(+) T cells. This review focuses on heterogeneity in CD8(+) T cell responses, particularly the impact of TCR stimulation strength and the mechanisms underlying variation between cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Tamar Nizharadze, Nils B. Becker, Thomas Hoefer
Summary: This study uses mathematical inference to establish quantitatively testable models of mammalian CD8+ T cell memory development based on complex experimental data. Previous inference studies have shown that precursors of memory T cells develop early in the immune response. Recent work has validated a crucial prediction of this T cell diversification model and refined the model.
TRENDS IN IMMUNOLOGY
(2023)
Review
Immunology
Marco Pio La Manna, Mojtaba Shekarkar Azgomi, Bartolo Tamburini, Giusto Davide Badami, Leila Mohammadnezhad, Francesco Dieli, Nadia Caccamo
Summary: This article examines the heterogeneity of the memory T cell compartment, with a particular focus on the emerging role of CD8(+) T-RM and T-SCM cells in the immune metabolism or modulation in chronic infections or autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Marzia Rossi, Andrea Vecchi, Camilla Tiezzi, Valeria Barili, Paola Fisicaro, Amalia Penna, Ilaria Montali, Stephane Daffis, Simon P. Fletcher, Anuj Gaggar, Jonathan Medley, Michael Graupe, Latesh Lad, Alessandro Loglio, Roberta Soffredini, Marta Borghi, Teresa Pollicino, Cristina Musolino, Arianna Alfieri, Federica Brillo, Diletta Laccabue, Marco Massari, Chiara Boarini, Gianluca Abbati, Giuseppe Pedrazzi, Gabriele Missale, Pietro Lampertico, Carlo Ferrari, Carolina Boni
Summary: This study characterized the functional impairment of exhausted HBV-specific CD8 T cells in patients with chronic HBV infection. It identified subsets of patients with different capacities to control infection and respond to immune modulation. It also found that functionally more efficient HBV-specific CD8 T cell subsets with lower expression of coinhibitory molecules were present in some chronic viraemic patients. The ability to distinguish patient cohorts with different responses to immune modulation through phenotypic CD8 T cell exhaustion profiling is clinically significant.
Review
Immunology
Fabienne Grabnitz, Annette Oxenius
Summary: Establishing cellular diversity is essential for the development of multicellular organisms. Asymmetric cell division (ACD) can induce cellular diversification by unevenly distributing lineage-specific cargo to daughter cells, resulting in different fates. The role of ACD in fate diversification of T-cells, specifically CD8 T-cells, is currently being investigated.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Cell Biology
Florian Schmidt, Hannah F. Fields, Yovita Purwanti, Ana Milojkovic, Syazwani Salim, Kan Xing Wu, Yannick Simoni, Antonella Vitiello, Daniel T. Macleod, Alessandra Nardin, Evan W. Newell, Katja Fink, Andreas Wilm, Michael Fehlings
Summary: In this study, high-dimensional mass cytometry and single-cell RNA sequencing were used to analyze CD8(+) T cells binding to different virus antigens. Phenotypic signatures of antigen-specific T cells were extracted using machine learning, which accurately predicted virus specificity for bulk CD8(+) T cells.
Article
Oncology
Yaoxin Zhang, Wenhui Li, Jiawei Zhai, Yujia Jin, Lianjun Zhang, Cheng Chen
Summary: This study analyzed the phenotype and cytokine secretion of CD8(+) T cells in malignant pleural effusions (MPE). The results showed that MPE-derived CD8(+) T cells expressed higher levels of PD1 and CD39 and had greater cytokine production compared to peripheral blood T cells. These findings highlight the potential of MPE-derived CD8(+) T cells as a target for immunotherapy.
EXPERIMENTAL CELL RESEARCH
(2022)
Review
Immunology
Joseph S. Dolina, Natalija Van Braeckel-Budimir, Graham D. Thomas, Shahram Salek-Ardakani
Summary: Recent research has revealed the heterogeneity within the exhausted CD8(+) T cell lineage, which consists of multiple interconnected subpopulations with distinct characteristics and locations. This understanding calls for a re-focusing of cancer immunotherapies on targeting the driver mechanisms underlying CD8(+) T(ex) development to stabilize functional subsets.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Zhi-Yong Shi, Sheng-Xiao Zhang, Di Fan, Cai-Hong Li, Zhe-Hao Cheng, Yan Xue, Li-Xiang Wu, Ke-Yi Lu, Su-Yun Yang, Yan Cheng, Zhi-Fang Wu, Chong Gao, Xiao-Feng Li, Hai-Yan Liu, Si-Jin Li
Summary: In this study, the effects of total thyroidectomy and radioactive iodine therapy on immune function in patients with differentiated thyroid carcinoma (DTC) were investigated. The results showed that both treatments caused changes in immune function, particularly in regulatory T cells and various lymphocyte subgroups. The percentage of Th17 cells before treatment may serve as a significant predictor of poor prognosis in DTC patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Midori Nakamura-Hoshi, Takushi Nomura, Masako Nishizawa, Trang Thi Thu Hau, Hiroyuki Yamamoto, Midori Okazaki, Hiroshi Ishii, Kenzo Yonemitsu, Yuriko Suzaki, Yasushi Ami, Tetsuro Matano
Summary: Human T-cell leukemia virus type 1 (HTLV-1) can proliferate from latently infected cells in the absence of CD8(+) cells, suggesting that CD8(+) cells are responsible for the control of HTLV-1 replication.
MICROBIOLOGY SPECTRUM
(2023)
Article
Rheumatology
Rosanne D. Reitsema, Kornelis S. M. van der Geest, Maria Sandovici, William F. Jiemy, Jacoba C. Graver, Wayel H. Abdulahad, Annemieke M. H. Boots, Peter Heeringa, Elisabeth Brouwer
Summary: Evidence suggests that CD8(+) T cells are involved in the pathogenesis of GCA, and this study provides a comprehensive analysis of how CD8(+) T cells function in GCA patients. The findings show that circulating CD8(+) T cells in GCA patients have a proliferation-prone phenotype and display altered gene expression. CD8(+) T cells are present in inflamed arteries and produce IFN-gamma, which plays a crucial role in the local inflammatory responses in GCA.
Article
Immunology
Emma C. Reilly, Mike Sportiello, Kris Lambert Emo, Andrea M. Amitrano, Rakshanda Jha, Ashwin B. R. Kumar, Nathan G. Laniewski, Hongmei Yang, Minsoo Kim, David J. Topham
Summary: CD8 T cell memory within lung tissue is highly heterogeneous, with various subsets such as T-CM, T-EM, T-RM, and populations not fitting neatly into these classifications. CD49a-expressing T-RM cells exhibit increased and diverse effector potential, functioning as a versatile group with antiviral activity, chemokine production, release of GM-CSF, and the ability to kill specific targets.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Lisa J. Sudmeier, Kimberly B. Hoang, Edjah K. Nduom, Andreas Wieland, Stewart G. Neill, Matthew J. Schniederjan, Suresh S. Ramalingam, Jeffrey J. Olson, Rafi Ahmed, William H. Hudson
Summary: Metastatic brain tumors are heavily infiltrated by CD8(+) T cell subsets with distinct antigen specificities, phenotypic states, and spatial localization within the tumor microenvironment. These subsets show minimal clonal overlap with circulating and other tumor-infiltrating CD8(+) T cells, and occupy discrete niches within the brain metastasis tumor microenvironment. This study provides potential targets for immunotherapy of brain metastases.
CELL REPORTS MEDICINE
(2022)
Article
Immunology
Namit Holay, Barry E. Kennedy, J. Patrick Murphy, Prathyusha Konda, Michael Giacomantonio, Tatjana Brauer-Chapin, Joao A. Paulo, Vishnupriyan Kumar, Youra Kim, Mariam Elaghil, Gary Sisson, Derek Clements, Christopher Richardson, Steven P. Gygi, Shashi Gujar
Summary: CD8 T cells are important in antiviral immunity. Type I interferons can cause bystander activation of CD8 T cells, including naive CD8 T cells, and promote their antiviral responses. This study found that exposure to reovirus can lead to bystander activation of naive CD8 T cells within 24 hours, and these cells display an innate, antiviral, interferon-sensitive signature. This novel aspect of CD8 T cell activation may have implications for understanding the immune response to virus infections.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Erietta Stelekati, Zhangying Cai, Sasikanth Manne, Zeyu Chen, Jean-Christophe Beltra, Lance Alec Buchness, Xuebing Leng, Svetlana Ristin, Kito Nzingha, Viktoriya Ekshyyan, Christina Niavi, Mohamed S. Abdel-Hakeem, Mohammed-Alkhatim Ali, Sydney Drury, Chi Wai Lau, Zhen Gao, Yuguang Ban, Simon K. Zhou, K. Mark Ansel, Makoto Kurachi, Martha S. Jordan, Alejandro V. Villarino, Shin Foong Ngiow, E. John Wherry
Summary: This study identifies miR-29a as a key regulator of exhausted CD8 T cells (TEX) during chronic viral infection. It shows that miR-29a improves CD8 T cell responses, inhibits exhaustion-driving transcriptional pathways, and regulates ribosomal biogenesis. As a result, miR-29a promotes a memory-like CD8 T cell differentiation state during chronic infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Rheumatology
Silvia Menegatti, Vincent Guillemot, Eleonora Latis, Hanane Yahia-Cherbal, Daniela Mittermueller, Vincent Rouilly, Elena Mascia, Nicolas Rosine, Surya Koturan, Gael A. Millot, Claire Leloup, Darragh Duffy, Aude Gleizes, Salima Hacein-Bey-Abina, Jeremie Sellam, Francis Berenbaum, Corinne Miceli-Richard, Maxime Dougados, Elisabetta Bianchi, Lars Rogge
Summary: Anti-TNF therapy induces significant changes in patients' innate immune responses, with selective action and minor effects on Th1/Th17 immunity. Immune response profiling provides new insights into the biology of TNF-blocker action and identifies signaling pathways associated with therapeutic responses to biological therapies.
ANNALS OF THE RHEUMATIC DISEASES
(2021)
Article
Multidisciplinary Sciences
Capucine L. Grandjean, Zacarias Garcia, Fabrice Lemaitre, Beatrice Breart, Philippe Bousso
Summary: It was found that macrophages in the bone marrow are the main way to eliminate tumors, but they can only act on neighboring tumors and have a limited active time.
Article
Biochemistry & Molecular Biology
Roxana Khazen, Marine Cazaux, Fabrice Lemaitre, Beatrice Corre, Zacarias Garcia, Philippe Bousso
Summary: The majority of CTL interactions with tumor cells either in vitro or in vivo result in sublethal hits, which are associated with reversible calcium elevation and membrane damage in the target cells. In the therapeutic context of anti-CD19 CAR T cells, most interactions with tumor cells do not lead to lethal hit delivery. Differences in CTL lytic potential and tumor cell resistance to cytotoxic hits are two important barriers for effective anti-tumor responses in vivo.
Article
Immunology
Morgane Boulch, Marine Cazaux, Yann Loe-Mie, Ronan Thibaut, Beatrice Corre, Fabrice Lemaitre, Capucine L. Grandjean, Zacarias Garcia, Philippe Bousso
Summary: Chimeric antigen receptor (CAR) T cell therapy relies on interactions with the tumor microenvironment (TME) for optimal activity, with IFN-gamma playing a crucial role in enhancing host immune response and sustaining CAR T cell cytotoxicity. CAR T cell-derived IFN-gamma facilitates host interleukin-12 production to support host immune and CAR T cell responses, highlighting the importance of cytokine-mediated cross-talk for effective CAR T cell therapy.
SCIENCE IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Margot Bardou, Jeremy Postat, Clemence Loaec, Fabrice Lemaitre, Gustave Ronteix, Zacarias Garcia, Philippe Bousso
Summary: Dendritic cell activation by viral RNA sensors is crucial for antiviral immunity, with type I interferon signaling playing a key role. Research shows that a quorum of type I interferon-producing cells is needed for optimal DC activation, with this collective behavior favored by the requirement for prolonged cytokine exposure. Collective DC activation is essential for the development of innate and adaptive immunity in lymph nodes, highlighting the role of collective processes in governing immune responses.
Article
Cell Biology
Mariana M. S. Oliveira, Shin-Yu Kung, Helene D. Moreau, Mathieu Maurin, Julien Record, Doriane Sanseau, Susanne Nylen, Ana-Maria Lennon-Dumenil, Lisa S. Westerberg
Summary: The study found that DCs lacking WASp or carrying the WASp L272P mutation exhibit different characteristics in migration and cell morphology. Compared to wild-type DCs, they show increased migration speed or higher speed fluctuations, but still exhibit differences in location within lymph nodes and cell structure.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Review
Rheumatology
Cindy Orvain, Morgane Boulch, Philippe Bousso, Yannick Allanore, Jerome Avouac
Summary: CAR-T cell therapy targets tumor antigens with high specificity, demonstrating efficacy in managing B cell malignancies. The technology has potential applications in autoimmune rheumatic diseases, with ongoing research exploring its use and impact in this area.
ARTHRITIS & RHEUMATOLOGY
(2021)
Review
Cell Biology
Ana-Maria Lennon-Dumenil, Helene D. Moreau
Summary: Cell migration plays a crucial role in physiological functions, being guided by both chemical and physical cues. Besides chemokines, the physical properties of tissues like hydraulic resistance also influence cell migration and may be involved in immune and cancer cell processes.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Immunology
Claudia A. Rivera, Violaine Randrian, Wilfrid Richer, Yohan Gerber-Ferder, Maria-Graciela Delgado, Aleksandra S. Chikina, Annika Frede, Chiara Sorini, Mathieu Maurin, Hana Kammoun-Chaari, Sara M. Parigi, Christel Goudot, Mar Cabeza-Cabrerizo, Sylvain Baulande, Sonia Lameiras, Pierre Guermonprez, Caetano Reis e Sousa, Marc Lecuit, Helene D. Moreau, Julie Helft, Danijela Matic Vignjevic, Eduardo J. Villablanca, Ana-Maria Lennon-Dumenil
Summary: There are two pools of cDC2s in the small intestine, originating from common pre-DC precursors, and their phenotypes are influenced by food-derived retinoic acid.
Review
Immunology
Philippe Bousso
Summary: Tumor immunotherapies like tumor-targeting monoclonal antibodies, immune checkpoint blockers, and CAR T cells have shown clinical successes and raised hopes, emphasizing the need for better understanding of their precise mechanisms in the patient body to optimize therapeutic efficacy. In this context, intravital two-photon imaging provides unique insights into the mode of action of these therapies and helps identify critical bottlenecks in vivo, contributing to the emergence of new concepts in anti-tumor immune responses.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Multidisciplinary Sciences
Gustave Ronteix, Shreyansh Jain, Christelle Angely, Marine Cazaux, Roxana Khazen, Philippe Bousso, Charles N. Baroud
Summary: The cytotoxic T cell responses to cancer vary greatly among individuals. By studying the stochastic modeling of high-throughput T cell behavior and matched tumor spheroid fate data generated by a microfluidics system, the authors show that tumor killing is dependent on T cell cooperativity, which may contribute to the heterogeneity of T cell responses.
NATURE COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Barthelemy Caron, Etienne Patin, Maxime Rotival, Bruno Charbit, Matthew L. Albert, Lluis Quintana-Murci, Darragh Duffy, Antonio Rausell
Summary: This study evaluated plasma protein levels in a cohort of 400 unrelated healthy individuals of western European ancestry and identified novel genetic and non-genetic factors associated with plasma protein levels.
Review
Oncology
Sonia Guedan, Maik Luu, Delphine Ammar, Paula Barbao, Chiara Bonini, Philippe Bousso, Christian J. Buchholz, Monica Casucci, Biagio De Angelis, Emmanuel Donnadieu, David Espie, Beatrice Greco, Richard Groen, Johannes B. Huppa, Chahrazade Kantari-Mimoun, Bruno Laugel, Mary Mantock, Janet L. Markman, Emma Morris, Concetta Quintarelli, Michael Rade, Kristin Reiche, Alba Rodriguez-Garcia, Juan Roberto Rodriguez-Madoz, Eliana Ruggiero, Maria Themeli, Michael Hudecek, Ibtissam Marchiq
Summary: Immunotherapy with gene engineered CAR and TCR transgenic T-cells is a groundbreaking treatment in cancer medicine, and has the potential to be used in common solid tumors. However, in order to improve and accelerate the selection of lead T-cell products for clinical translation, it is necessary to assess preclinical models and develop new models with higher predictive value.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Immunology
Valeria Fumagalli, Valentina Venzin, Pietro Di Lucia, Federica Moalli, Xenia Ficht, Gioia Ambrosi, Leonardo Giustini, Francesco Andreata, Marta Grillo, Diletta Magini, Micol Rava, Christin Friedrich, Jason D. Fontenot, Philippe Bousso, Sarah A. Gilmore, Shahzada Khan, Manuel Baca, Eric Vivier, Georg Gasteiger, Mirela Kuka, Luca G. Guidotti, Matteo Iannacone
Summary: Group 1 innate lymphoid cells (ILCs) play a role in controlling T cell-mediated liver immunopathology by limiting local IL-2 concentration.
SCIENCE IMMUNOLOGY
(2022)
Article
Oncology
Ronan Thibaut, Pierre Bost, Idan Milo, Marine Cazaux, Fabrice Lemaitre, Zacarias Garcia, Ido Amit, Beatrice Breart, Clemence Cornuot, Benno Schwikowski, Philippe Bousso