Article
Biochemistry & Molecular Biology
Jigar Desai, Dhaneshwar Kumar, Tilo Freiwald, Daniel Chauss, Melissa D. Johnson, Michael S. Abers, Julie M. Steinbrink, John R. Perfect, Barbara Alexander, Vasiliki Matzaraki, Brendan D. Snarr, Marissa A. Zarakas, Vasileios Oikonomou, Lakmali M. Silva, Raju Shivarathri, Emily Beltran, Luciana Negro Demontel, Luopin Wang, Jean K. Lim, Dylan Launder, Heather R. Conti, Muthulekha Swamydas, Micah T. McClain, Niki M. Moutsopoulos, Majid Kazemian, Mihai G. Netea, Vinod Kumar, Jorg Kohl, Claudia Kemper, Behdad Afzali, Michail S. Lionakis
Summary: Systemic candidiasis, a common and high-mortality nosocomial fungal infection, can be a complication of anti-complement C5-targeted monoclonal antibody treatment, which highlights the important role of C5 in antifungal immunity. Transcription of complement system genes was found to be the top biological pathway induced in candidemic patients and predictive of candidemia. Mechanistically, C5a-C5aR1 promotes fungal clearance and host survival in a mouse model of systemic candidiasis by enhancing phagocyte effector function and ensuring survival in infected tissues. Local production of C5 by phagocytes provides a crucial substrate for antifungal protection.
Article
Cell Biology
Fuquan Wang, Ming Chen, Chenchen Wang, Haifa Xia, Dingyu Zhang, Shanglong Yao
Summary: Maresin1 plays an important role in regulating neutrophils and has potential value in the treatment of septic lung injury. Using single-cell sequencing technology, this study revealed for the first time the regulatory mechanism of Maresin1 on neutrophils.
Article
Endocrinology & Metabolism
Ning Ma, Weidong Wu
Summary: This study investigated the transcriptomic responses to septic shock and cardiogenic shock and identified differentially expressed genes and related molecular mechanisms. A total of 28 genes were found to be differentially expressed in both types of shock, and specific genes associated with septic shock were identified. The study provides a basis for distinguishing between septic shock and cardiogenic shock, improving diagnosis and guiding treatment choices.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Health Care Sciences & Services
Athanasios Chalkias, Eleni Laou, Nikolaos Papagiannakis, Vaios Spyropoulos, Evaggelia Kouskouni, Kassiani Theodoraki, Theodoros Xanthos
Summary: The present study investigated the changes in stressed volume (Vs) and other determinants of venous return during hyperdynamic septic shock. The results showed that the baseline Vs decreased by 7% for each mmHg decrease in MAP. Significant changes were also observed in other determinants of venous return.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Anitra C. Carr, Stephanie Bozonet, Juliet Pullar, Emma Spencer, Patrice Rosengrave, Geoff Shaw
Summary: Despite septic patients having significantly lower plasma ascorbate status, the intracellular ascorbate content in neutrophils from septic patients is comparable to that of healthy controls, while erythrocytes show lower content. The mechanisms behind this remain uncertain, potentially involving increased generation of dehydroascorbic acid.
Article
Medicine, General & Internal
Paolo Persona, Ilaria Valeri, Elisabetta Saraceni, Alessandro De Cassai, Fabrizia Calabrese, Paolo Navalesi
Summary: The study compared the agreement between MostCare and PiCCO systems in detecting changes in cardiac output in septic shock patients. The results showed good consistency between the two systems after reducing norepinephrine infusion, suggesting that MostCare may be an effective tool for monitoring hemodynamic changes in emergency situations.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Pediatrics
Nicholas W. Miller, Brittany G. Seman, Stephen M. Akers, Jessica M. Povroznik, Kathleen Brundage, Wei Fang, Cory M. Robinson
Summary: This study is the first to evaluate the impact of opioid exposure on neonatal immune profile and functionality, and found differences in immune cell populations, cytokine levels, and functional activity in neonates exposed to opioids, which may increase susceptibility to infections.
PEDIATRIC RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Jeffrey Wang, Willard N. Applefeld, Junfeng Sun, Steve B. Solomon, Jing Feng, Zoe G. Couse, Thomas F. Risoleo, Robert L. Danner, Jesus Tejero, Juan Lertora, Elmira Alipour, Swati Basu, Vandana Sachdev, Daniel B. Kim-Shapiro, Mark T. Gladwin, Harvey G. Klein, Charles Natanson
Summary: The study found that elevated CFH levels in septic shock lead to various pathophysiological changes, including increased pulmonary arterial pressures and right ventricular afterload, depressed cardiac function, worsening shock, lactate levels, metabolic acidosis, and multiorgan failure. These effects are mainly attributed to nitric oxide scavenging and iron supply promoting bacterial growth.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Xiaoyuan Yang, Ethan Zheng, Yonggang Ma, Victor Chatterjee, Nuria Villalba, Jerome W. Breslin, Ruisheng Liu, Mack H. Wu, Sarah Y. Yuan
Summary: Renal dysfunction is a common complication of septic injury, with protein palmitoylation playing a regulatory role in vascular function. The deficiency of DHHC21 can attenuate renal pathology induced by CLP, leading to improved renal perfusion and oxygen saturation. DHHC21 contributes to impaired renal perfusion and function during septic injury by promoting alpha 1AR palmitoylation-associated vasoconstriction.
SCIENTIFIC REPORTS
(2021)
Article
Biophysics
S. A. McErlain-Naylor, M. A. King, S. J. Allen
Summary: This study quantified the surface-measured accelerations at major human body segments during impact landings and found that accelerations decreased from the foot to the torso, with no further attenuation beyond the L5 vertebra. Active and passive mechanisms within the lower limb contribute to progressive attenuation of accelerations, preventing excessive accelerations from reaching the torso and head.
JOURNAL OF BIOMECHANICS
(2021)
Article
Immunology
Stephanie L. Silva-Del Toro, Lee-Ann H. Allen
Summary: Neutrophils, also known as PMNs, show heterogeneous characteristics and considerable plasticity, as demonstrated by the induction of N1-like subtype differentiation in H. pylori infection. The study revealed that microtubule dynamics and dynein activity are essential for the hypersegmentation of the nucleus, with an increase in microtubule abundance and length in hypersegmented cells. These findings advance the understanding of nuclear morphology regulation processes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Qiang Wang, Jin Deng, Jianhui Sun, Huacai Zhang, Di Liu, Chu Gao, Jinchao Qiu, Wenyi Liu, Guoxin Qu, Dalin Wen, Juan Du, Anqiang Zhang, Ling Zeng, Jianxin Jiang
Summary: Studies have shown that CP-673451, a PDGFR kinase inhibitor, may effectively treat sepsis by inhibiting lymphocyte chemotaxis and reducing cytokine storm.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Charles A. Warwick, Alex L. Keyes, Trent M. Woodruff, Yuriy M. Usachev
Summary: The complement cascade plays a crucial role in the recognition and clearance of pathogens and tissue repair in the innate immune system. Recent studies have revealed unexpected roles of complement cascade in neuroimmune interaction and neuronal processes regulation, particularly in directing neuronal responses to injury. Dysregulation of complement cascade can lead to chronic inflammation, persistent pain, and neural dysfunction, while complement-dependent changes promote nerve regeneration and tissue repair.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Immunology
Marcela Hortova-Kohoutkova, Monika Skotakova, Isaac G. Onyango, Miriam Slezakova, Roman Panovsky, Lukas Opatril, Peter Slanina, Marco De Zuani, Ondrej Mrkva, Ivana Andrejcinova, Petra Laznickova, Martina Dvoncova, Alexandra Mytnikova, Vaughn Ostland, Michal Sitina, Gorazd B. Stokin, Vladimir Sramek, Marcela Vlkova, Martin Helan, Jan Fric
Summary: This study investigated the dynamics of iron regulation in different clinical settings and determined the suitability of hepcidin and/or ferritin levels as biomarkers of inflammatory disease severity. The results showed that hepcidin levels reflect overall immune cell activation driven by intrinsic stimuli, while ferritin levels are more strongly influenced by pathogen-induced inflammation. Furthermore, the hepcidin-to-ferritin ratio has the ability to predict mortality in septic shock.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Fanny Vardon-Bounes, Cedric Garcia, Alexandra Piton, Jennifer Series, Marie-Pierre Gratacap, Michael Poette, Thierry Seguin, Laure Crognier, Stephanie Ruiz, Stein Silva, Jean-Marie Conil, Vincent Minville, Bernard Payrastre
Summary: This study assessed platelet activation parameters at admission and 48 hours later in patients with sepsis, revealing significantly increased platelet activation levels in patients compared to controls at both time points, but with less responsiveness to different agonists.
Article
Multidisciplinary Sciences
Korbinian Kienle, Katharina M. Glaser, Sarah Eickhoff, Michael Mihlan, Konrad Knoepper, Eduardo Reategui, Maximilian W. Epple, Matthias Gunzer, Ralf Baumeister, Teresa K. Tarrant, Ronald N. Germain, Daniel Irimia, Wolfgang Kastenmueller, Tim Laemmermann
Summary: The study found that neutrophils have evolved an intrinsic mechanism to limit self-aggregation during inflammation, with GPCR desensitization acting as a negative feedback control to stop migration when high concentrations of attractants are detected. However, interfering with this process may hinder bacterial clearance, as bacteria proliferation within neutrophil clusters becomes impeded. This highlights the delicate balance of neutrophil chemotaxis and arrest in combating bacterial escape.
Article
Cell Biology
Lynda J. Partridge, Lucy Urwin, Martin J. H. Nicklin, David C. James, Luke R. Green, Peter N. Monk
Summary: The spike protein of SARS-CoV-2 binds primarily to ACE2 on target cells but may also involve other receptors. Heparin and glycosaminoglycans such as heparan sulfate and dermatan sulfate can inhibit the binding of spike protein to cells, indicating potential interactions with host cells beyond ACE2.
Review
Cell Biology
Katharina M. Glaser, Michael Mihlan, Tim Laemmermann
Summary: This article discusses the positive feedback amplification underlying immune cell swarming, which is caused by intercellular communication within the cell population. Studies using mouse models, zebrafish larvae, and in vitro platforms for human cells have advanced our understanding of the principles and molecular mechanisms shaping immune cell swarming.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Katherine S. Marsay, Sarah Greaves, Harsha Mahabaleshwar, Charmaine Min Ho, Henry Roehl, Peter N. Monk, Tom J. Carney, Lynda J. Partridge
Summary: Collective cell migration of the posterior lateral line primordium in zebrafish development is modulated by Cd9, which contributes to effective chemokine signaling. Cd9a and Cd9b function partially redundantly in pLLP migration, with their loss leading to delayed migration and reduced neuromast numbers. Loss of both Cd9a and Cd9b sensitizes embryos to reduced levels of Cxcr4b and Cxcl12a.
Article
Multidisciplinary Sciences
Felix Juenger, Dominic Ruh, Dominik Strobel, Rebecca Michiels, Dominik Huber, Annette Brandel, Josef Madl, Alina Gavrilov, Michael Mihlan, Caterina Cora Daller, Eva A. Rog-Zielinska, Winfried Roemer, Tim Laemmermann, Alexander Rohrbach
Summary: Fluorescence techniques dominate live-cell microscopy, but long integration times result in bleaching and motion blur that limits dynamic investigations. The authors introduce Rotating Coherent Scattering (ROCS) microscopy, a label-free high contrast imaging method that combines intensity speckle patterns from all illumination directions. With 160 nm resolution and 100 Hz speed, ROCS microscopy allows for imaging thousands of acquisitions in live cells. The authors demonstrate the performance of improved Total Internal Reflection (TIR)-ROCS in combination with fluorescence, and use time-window analysis methods to understand the effects of motion blur on cellular structures and slow structure motions.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Michael Mihlan, Katharina M. Glaser, Maximilian W. Epple, Tim Laemmermann
Summary: Neutrophils are key cells of our immune response that play essential roles in eliminating bacteria and fungi from tissues. They are highly mobile, moving in a substrate-independent manner guided by chemokines and chemoattractants in the tissue. Neutrophils can sense danger signals and communicate with each other, amplifying their recruitment to sites of tissue injury and infection. Understanding the negative feedback control mechanisms that terminate neutrophil swarming is crucial for maintaining the delicate balance between tissue surveillance, host protection, and tissue destruction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Michael Mihlan, Shima Safaiyan, Manuel Stecher, Neil Paterson, Tim Laemmermann
Summary: Successful immune responses rely on the coordinated migration, interactions, and functioning of immune cells in lymphoid and parenchymal tissues. Real-time intravital microscopy has provided insights into the dynamic behavior of immune cells in living tissues, revealing unexpected biological features that were not observed in experiments outside of a living organism.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Medicine, General & Internal
Naveen Kumar Devanga Ragupathi, Balaji Veeraraghavan, Esther Karunakaran, Peter N. Monk
FRONTIERS IN MEDICINE
(2022)
Article
Multidisciplinary Sciences
Sarah Greaves, Katherine S. Marsay, Peter N. Monk, Henry Roehl, Lynda J. Partridge
Summary: CD9 expression is important for sperm-egg fusion in mice, but its role in fish fertility is more complex. Knockout of cd9b in zebrafish leads to decreased egg numbers and fertilization rates. These effects are not due to alterations in primordial germ cells. The fertility defects can be rescued by crossing with wildtype partners. In vitro fertilization experiments confirm the reduced egg numbers and fertilization rates in cd9b mutants are not solely due to mating behavior. CD9 plays a role in fish fertility in both males and females.
Article
Immunology
Lukas Kaltenbach, Paloma Martzloff, Sarah K. Bambach, Nadim Aizarani, Michael Mihlan, Alina Gavrilov, Katharina M. Glaser, Manuel Stecher, Roland Thuenauer, Aude Thiriot, Klaus Heger, Katrin Kierdorf, Stephan Wienert, Ulrich H. von Andrian, Marc Schmidt-Supprian, Claus Nerlov, Frederick Klauschen, Axel Roers, Marc Bajenoff, Dominic Gruen, Tim Laemmermann
Summary: The movement of mast cells differs from the general concept of interstitial immune cell migration. Integrin-dependent adhesion plays a crucial role in controlling mast cell movement and localization. Substrate-dependent haptokinesis is important for the tissue organization of resident immune cells.
Article
Microbiology
Luke R. R. Green, Rahaf Issa, Fawzyah Albaldi, Lucy Urwin, Ruth Thompson, Henna Khalid, Claire E. E. Turner, Barbara Ciani, Lynda J. J. Partridge, Peter N. N. Monk
Summary: This article investigates the role of the host membrane adapter protein CD9 in Staphylococcus aureus adhesion to epithelial cells. The study demonstrates that CD9 is essential for syndecan-mediated bacterial adhesion and that it coordinates with fibronectin to promote bacterial adherence and internalization. Insights from this research may lead to the development of alternative anti-adhesion treatments for staphylococcal infections.
Article
Multidisciplinary Sciences
Dhammika Leshan, Aye Mya Sithu Shein, Cameron Hurst, Peter N. Monk, Parichart Hongsing, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Pattama Wapeesittipan, Sirirat Luk-in, Sasipen Sae-Joo, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Naris Kueakulpattana, Matchima Laowansiri, Tingting Liao, Rosalyn Kupwiwat, Rojrit Rojanathanes, Natharin Ngamwongsatit, Somkanya Tungsanga, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Vishnu Nayak Badavath, S. M. Ali Hosseini, Talerngsak Kanjanabuch, Nattiya Hirankarn, Robin James Storer, Longzhu Cui, Mohan Amarasiri, Hitoshi Ishikawa, Paul G. Higgins, Stephen M. Stick, Anthony Kicic, Tanittha Chatsuwan, Shuichi Abe
Summary: The novel aztreonam-Ca-EDTA or ceftazidime-avibactam-Ca-EDTA combinations show favorable efficacy and safety in treating carbapenemase-positive Klebsiella pneumoniae (CPKp) infections.
Article
Medicine, Research & Experimental
Aye Mya Sithu Shein, Dhammika Leshan Wannigama, Cameron Hurst, Peter N. Monk, Mohan Amarasiri, Vishnu Nayak Badavath, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Wanwara Thuptimdang, Sirirat Luk-in, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Naris Kueakulpattana, Matchima Laowansiri, Tingting Liao, Rosalyn Kupwiwat, Rojrit Rojanathanes, Natharin Ngamwongsatit, Arsa Thammahong, Hitoshi Ishikawa, Daniel Pletzer, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Pattama Wapeesittipan, S. M. Ali Hosseini Rad, Talerngsak Kanjanabuch, Robin James Storer, Kazuhiko Miyanaga, Longzhu Cui, Hiroshi Hamamoto, Paul G. Higgins, Anthony Kicic, Tanittha Chatsuwan, Parichart Hongsing, Shuichi Abe
Summary: This study tested the efficacy of a combination therapy using phage and an adjuvant in treating multidrug-resistant Pseudomonas aeruginosa infections. The results showed that intranasal inhalation of phage along with CaEDTA successfully resensitized the bacteria to specific antibiotics, indicating its potential as a therapy for respiratory tract infections.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Endocrinology & Metabolism
Ev-Marie Schuster, Maximilian W. Epple, Katharina M. Glaser, Michael Mihlan, Kerstin Lucht, Julia A. Zimmermann, Anna Bremser, Aikaterini Polyzou, Nadine Obier, Nina Cabezas-Wallscheid, Eirini Trompouki, Andrea Ballabio, Joerg Vogel, Joerg M. Buescher, Alexander J. Westermann, Angelika S. Rambold
Summary: There is a metabolic pathway regulated by TFEB between phago-lysosomes and mitochondria in macrophages, which restricts the growth of Salmonella bacteria by producing itaconate.
Meeting Abstract
Respiratory System
Dhammika Leshan Wannigama, Cameron Hurst, Peter N. Monk, Shuichi Abe, Gunter Hartel, Paul G. Higgins, Stephen M. Stick, Anthony Kicic, Jane Davies, Tanittha Chatsuwan