Article
Biochemistry & Molecular Biology
Masato Mizuno, Shuhei Ebine, Okuto Shounai, Shizuka Nakajima, Shota Tomomatsu, Ken Ikeuchi, Yoshitaka Matsuo, Toshifumi Inada
Summary: This study reveals that polytryptophan sequences can induce Rqc2-independent ribosome-associated quality control (RQC), and this process does not require CAT-tailing. Polytryptophan sequences also induce ribosome-associated quality control coupled with canonical no-go mRNA decay (NGD) and ribosome-associated quality control uncoupled with NGD.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Cell Biology
Manuel Olazabal-Moran, Miriam Sanchez-Ortega, Laura Martinez-Munoz, Carmen Hernandez, Manuel S. Rodriguez, Mario Mellado, Ana C. Carrera
Summary: PI3-kinase and PTEN regulate the PIP3/AKT signaling pathway through the ubiquitin ligase cCBL, influencing cell survival, division, and migration. These findings reveal a novel mechanism for PI3-kinase/PTEN crosstalk and suggest that cCBL may be a potential target for modulating PTEN activity in cancer.
Article
Multidisciplinary Sciences
Xiaolan Liu, Xufeng Cen, Ronghai Wu, Ziyan Chen, Yanqi Xie, Fengqi Wang, Bing Shan, Linghui Zeng, Jichun Zhou, Bojian Xie, Yangjun Cai, Jinyan Huang, Yingjiqiong Liang, Youqian Wu, Chao Zhang, Dongrui Wang, Hongguang Xia
Summary: This study reveals that cisplatin enhances the anti-tumor effect of PD-L1 blockade and upregulates ARIH1 expression, promoting T cell infiltration and sensitizing tumors to immune checkpoint blockade. ARIH1 mediates activation of the STING pathway by ubiquitination and degradation of DNA-PKcs, which is a mechanism of tumor resistance to ICB. Activating ARIH1 is an effective strategy to improve the efficacy of cancer immunotherapy.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hang Chen, Rui Yang, Lei Xing, Bin Wang, Dawei Liu, Xiaoqiang Ou, Yumei Deng, Rong Jiang, Junxia Chen
Summary: This study identified a novel hypoxia-induced circRNA, circPFKFB4, and demonstrated its role in promoting breast cancer progression. The study revealed an interaction between circPFKFB4 and the CRL4DDB2 E3 ubiquitin ligase and suggested circPFKFB4 as a potential biomarker and therapeutic target for breast cancer.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Immunology
Jing Song, Warren Anderson, Alex Hu, Kazushige Obata-Ninomiya, Steven F. Ziegler, David J. Rawlings, Jane H. Buckner
Summary: This study utilized gene editing to knock out the CBLB gene in human CD4+ T cells, revealing that CBLB knockout resulted in hyperproliferation and excessive IL-2 production in CD4+ T cells. The knockout cells showed resistance to Treg suppression and were able to overcome Treg inhibition by producing high levels of cytokines, promoting the proliferation and activation of T effector cells.
JOURNAL OF IMMUNOLOGY
(2022)
Review
Cell Biology
Yi Xiong, Chao Yu, Qianting Zhang
Summary: This review discusses the functions of ubiquitin proteasome system (UPS) components in mammalian spermatogenesis, including the composition of proteasome isoforms at each stage, the specificity and associated degradation events of each isoform, E3 ubiquitin ligases mediating protein ubiquitination, and the deubiquitinases involved in spermatogenesis and male fertility. Exploring the UPS machineries in spermatogenesis provides insights into protein dynamics during male germ cell production and sheds light on the etiology and pathogenesis of human male infertility.
Article
Multidisciplinary Sciences
Ali Inayat Mallano, Zaib-un Nisa, Binish Khaliq, Naila Ali, Qurban Ali, Chen Chao, Zhu Yanming
Summary: This study isolated and characterized the GsPUB8 protein gene from wild soybean to investigate its response to abiotic stress. The GsPUB8 protein showed higher expression in roots under saline and drought stress, and was found to contain a U-box domain.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
M. Heider, Ruth Eichner, Jacob Stroh, Volker Morath, Anna Kuisl, Jana Zecha, Jannis Lawatscheck, Kheewoong Baek, Anne-Kathrin Garz, Martina Rudelius, Friedrich-Christian Deuschle, Ulrich Keller, Simone Lemeer, Mareike Verbeek, Katharina S. Gotze, Arne Skerra, Wolfgang A. Weber, Johannes Buchner, Brenda A. Schulman, Bernhard Kuster, Vanesa Fernandez-Saiz, Florian Bassermann
Summary: This study identifies the role of the CRBN-AHA1-HSP90 axis in the biogenesis of transmembrane proteins, linking IMiD activity to tumor metabolism, and nominating CD98hc and LAT1 as attractive diagnostic and therapeutic targets in MM.
Article
Biochemistry & Molecular Biology
Fang Liu, Wei Yang, Minghui Hu, Yong Zhang, Beicheng Sun, Hao Yang, Juergen Brosius, Cheng Deng
Summary: This study identifies a group of GPCRs that exhibit constitutive activity and play potentially important roles in physiological functions through rapid ubiquitin-dependent degradation, with GPR26 showing anti-tumor activity in liver cancer cells.
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Chemistry, Medicinal
Adam G. Bond, Conner Craigon, Kwok-Ho Chan, Andrea Testa, Athanasios Karapetsas, Rotimi Fasimoye, Thomas Macartney, J. Julian Blow, Dario R. Alessi, Alessio Ciulli
Summary: This study describes the design and development of a new protein degradation system utilizing a variant of the Brd4 bromodomain as a degradation tag. The system effectively degrades BromoTagged proteins in a fast, selective manner, showing favorable pharmacokinetic profile in mice. This system expands the arsenal of chemical genetic degradation tools for manipulating protein levels and exploring therapeutic potential in cells and in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Martin P. Schwalm, Lena M. Berger, Maximilian N. Meuter, James D. Vasta, Cesear R. Corona, Sandra Roehm, Benedict-Tilman Berger, Frederic Farges, Sebastian M. Beinert, Franziska Preuss, Viktoria Morasch, Vladimir V. Rogov, Sebastian Mathea, Krishna Saxena, Matthew B. Robers, Susanne Mueller, Stefan Knapp
Summary: E3 ligases play a crucial role in regulating protein homeostasis by recruiting substrate proteins to the proteasomal degradation machinery. Recent research has focused on the Baculovirus IAP Repeat (BIR) family of E3 ligases, which contain a structurally conserved but diverse protein interaction domain. The Inhibitors of Apoptosis (IAP) family, which typically have three BIR domains, are promising drug targets. However, there is currently a lack of assay tools to evaluate the selectivity of inhibitors in this target area.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rui Zhang, Shaoqing Shi
Summary: HECT-type E3 ubiquitin ligases play a vital role in controlling protein function and stability, and members of the NEDD4 family have critical roles in dysregulation of autophagy in cancer cells. This review focuses on the role of NEDD4 E3 ligases in defective autophagy in cancer cells, discussing their function, substrates, and signaling pathways, providing a basis for cancer treatment through modulation of these ligases.
MOLECULAR MEDICINE
(2023)
Review
Immunology
Haoran Cui, Yaxian Zhang, Leiliang Zhang
Summary: Poxviruses have evolved various mechanisms to evade innate immunity, some of which involve poxvirus-encoded E3 ubiquitin ligases and adaptor proteins. These proteins can be categorized into five groups based on their functional domains and ubiquitin transfer mechanisms. Most known substrates of poxvirus E3 ubiquitin ligases are components of the innate immune system. Current research progress provides mechanistic insights into the interaction between these viruses and their hosts.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Jialing Zhang, Chaonan Li, Long Li, Yajun Xi, Jingyi Wang, Xinguo Mao, Ruilian Jing
Summary: TaAIRP2-1B regulates spike length by facilitating TaHIPP3 degradation, and the haplotype Hap-1B-1 of TaAIRP2-1B is a favorable natural variation for increasing spike length in wheat. This study provides insights into the role of E3 ubiquitin ligase genes in wheat development and identifies TaAIRP2-1B as a key regulator of spike length. The findings also offer genetic resources and markers for wheat molecular breeding.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Immunology
Lifen Yang, Guilin Qiao, Yassir Hassan, Zhenping Li, Xiaoqing Zhang, Huimin Kong, Weimin Zeng, Fei Yin, Jian Zhang
ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS
(2016)
Article
Cell Biology
Cheng Song, Lujuan He, Jin Zhang, Hong Ma, Xiangning Yuan, Gaoyun Hu, Lijian Tao, Jian Zhang, Jie Meng
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2016)
Article
Immunology
Patrick K. Nuro-Gyina, Elizabeth L. Rieser, Marissa C. Granitto, Wei Pei, Yue Liu, Priscilla W. Lee, Saba Aqel, Jian Zhang, Amy E. Lovett-Racke, Michael K. Racke, Yuhong Yang
JOURNAL OF NEUROINFLAMMATION
(2016)
Article
Biochemistry & Molecular Biology
Yun Xiao, Juan Tang, Hui Guo, Yixia Zhao, Rong Tang, Song Ouyang, Qiuming Zeng, Chad A. Rappleye, Murugesan V. S. Rajaram, Larry S. Schlesinger, Lijian Tao, Gordon D. Brown, Wallace Y. Langdon, Belinda T. Li, Jian Zhang
Review
Immunology
Juan Tang, Guoxin Lin, Wallace Y. Langdon, Lijian Tao, Jian Zhang
FRONTIERS IN IMMUNOLOGY
(2018)
Review
Cell Biology
Rong Tang, Wallace Y. Langdon, Jian Zhang
CELLULAR IMMUNOLOGY
(2019)
Article
Immunology
Song Ouyang, Qiuming Zeng, Na Tang, Hui Guo, Rong Tang, Weifan Yin, Aimin Wang, Hongyu Tang, Jiru Zhou, Hong Xie, Wallace Y. Langdon, Huan Yang, Jian Zhang
JOURNAL OF IMMUNOLOGY
(2019)
Article
Immunology
Victoria Y. Kim, Abel Batty, Jinhui Li, Sean G. Kirk, Sara A. Crowell, Yi Jin, Juan Tang, Jian Zhang, Lynette K. Rogers, Han-Xiang Deng, Leif D. Nelin, Yusen Liu
JOURNAL OF IMMUNOLOGY
(2019)
Article
Immunology
Juan Tang, Sha Tu, Guoxin Lin, Hui Guo, Chengkai Yan, Qingjun Liu, Ling Huang, Na Tang, Yizhi Xiao, R. Marshall Pope, Murugesan V. S. Rajaram, Amal O. Amer, Brian M. Ahmer, John S. Gunn, Daniel J. Wozniak, Lijian Tao, Vincenzo Coppola, Liwen Zhang, Wallace Y. Langdon, Jordi B. Torrelles, Stanley Lipkowitz, Jian Zhang
JOURNAL OF EXPERIMENTAL MEDICINE
(2020)
Article
Immunology
Shailesh K. Shahi, Samantha N. Jensen, Alexandra C. Murra, Na Tang, Hui Guo, Katherine N. Gibson-Corley, Jian Zhang, Nitin J. Karandikar, Joseph A. Murray, Ashutosh K. Mangalam
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Immunology
Patrick K. Nuro-Gyina, Na Tang, Hui Guo, Chengkai Yan, Qiuming Zeng, Thomas J. Waldschmidt, Jian Zhang
Summary: The study revealed the essential role of Nedd4 in signaling through Dectin-1 and Dectin-2/3, with Nedd4-deficient mice showing increased susceptibility to systemic C. albicans infection. The molecular mechanisms involve impaired activation of TGF-beta-activating kinase-1 and NF-kappa B in macrophages lacking Nedd4, highlighting the regulatory role of Nedd4 in antifungal immunity.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Sean G. Kirk, Parker R. Murphy, Xiantao Wang, Charles J. Cash, Timothy J. Barley, Bridget A. Bowman, Abel J. Batty, William E. Ackerman, Jian Zhang, Leif D. Nelin, Markus Hafner, Yusen Liu
Summary: Mkp-1-deficient mice show elevated inflammatory cytokines, increased mortality, and disease severity following E. coli infection. Further investigation reveals that Mkp-1 regulates IFN-beta production primarily through a p38-mediated mechanism, and IFN-beta plays a beneficial role in E. coli-induced sepsis.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Juan Tang, Yizhi Xiao, Guoxin Lin, Hui Guo, Han-Xiang Deng, Sha Tu, Wallace Y. Langdon, Huixiang Yang, Lijian Tao, Yalan Li, R. Marshall Pope, Neetu Gupta, Jian Zhang
Summary: Research shows that Src family kinase Lyn regulates the activity of NLRP3 in immune cells by phosphorylating it, leading to its degradation and dampening the activity of the inflammasome.
Article
Biochemistry & Molecular Biology
Na Tang, Lifen Yang, Dongdong Li, Rushi Liu, Jian Zhang
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2019)
Review
Immunology
Jinghua Yin, Jian Zhang, Qianjin Lu
CLINICAL IMMUNOLOGY
(2017)