期刊
JOURNAL OF IMMUNOLOGY
卷 191, 期 10, 页码 5005-5012出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1300787
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资金
- Dutch Cancer Society [KUN2006-3699, KUN2009-4402]
- European Union [ENCITE HEALTH-F5-2008-201842, ERC-2010-AdG-269019, Pharmachild FP7-HEALTH-2010-260353]
- Netherlands Organisation for Scientific Research [NWO-Vidi-917.76.363]
- Nijmeegs Offensief tegen Kanker foundation
- Netherlands Institute for Regenerative Medicine [FES0908]
- Radboud University Nijmegen Medical Centre Ph.D. grant
- Netherlands Organisation for Scientific Research Spinoza award
Plasmacytoid dendritic cells (pDCs) play a crucial role in initiating immune responses by secreting large amounts of type I IFNs. Currently, the role for human pDCs as professional APCs in the cross-presentation of exogenous Ags is being re-evaluated. Human pDCs are equipped with a broad repertoire of Ag uptake receptors and an efficient Ag-processing machinery. In this study, we set out to investigate which receptor can best be deployed to deliver Ag to pDCs for Ag (cross-) presentation. We show that targeting nanoparticles to pDCs via the C-type lectins DEC-205, DC immunoreceptor, blood DC Ag-2, or the FcR CD32 led to uptake, processing, and (cross-) presentation of encapsulated Ag to both CD4(+) and CD8(+) T cells. This makes these receptors good candidates for potential in vivo targeting of pDCs by nanocarriers. Notably, the coencapsulated TLR7 agonist R848 efficiently activated pDCs, resulting in phenotypical maturation as well as robust IFN-alpha and TNF-alpha production. Taken together, their cross-presentation capacity and type I IFN production to further activate components of both the innate and adaptive immune system mark pDCs as inducers of potent antitumor responses. These findings pave the way to actively recruit human pDCs for cellular cancer immunotherapy.
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