Article
Medicine, Research & Experimental
Rachel Liuqing Shi, Gang Xiao, Thomas M. Dillon, Arnold McAuley, Margaret S. Ricci, Pavel V. Bondarenko
Summary: Chemical modifications in stressed therapeutic proteins can affect binding to target and efficacy. Competitive SEC and peptide mapping can reveal critical residues and modifications in antibody-target binding.
Article
Chemistry, Multidisciplinary
Hye Jin Kim, Dongsung Park, Yejin Park, Dae-Hyeong Kim, Jinsik Kim
Summary: This study developed a biosensor with enhanced antibody-antigen binding probability by applying an electric field to align the orientation of polar antibodies. The results showed that this method effectively maximized the sensitivity of the biosensor by optimizing the alignment condition and aligning the antibodies on the sensor surface.
Article
Medicine, Research & Experimental
Pavel Bondarenko, Andrew C. Nichols, Gang Xiao, Rachel Liuqing Shi, Pik Kay Chan, Thomas M. Dillon, Fernando Garces, David J. Semin, Margaret S. Ricci
Summary: The article introduces a method for large-scale, parallel identification of all critical chemical modifications in therapeutic proteins. By stressing the antibodies, separating them, and analyzing the fractions, it can effectively assess the quality of the proteins. This method is more sensitive to low-level modifications and suitable for quickly evaluating the impact of modifications on binding.
Article
Immunology
Robert L. Atmar, Khalil Ettayebi, Sasirekha Ramani, Frederick H. Neill, Lisa Lindesmith, Ralph S. Baric, Amanda Brinkman, Ralph Braun, James Sherwood, Mary K. Estes
Summary: In an outbreak of GII.2, researchers investigated the immune response to vaccination and infection of both GII.4 homotypic and GII.2 heterotypic strains. The results showed that the vaccine provided protection from GII.2-associated illness, but there was little GII.2-specific neutralization response after vaccination. The choice of antibody assay can affect the assessment of vaccine immunogenicity.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Kristen A. Rodrigues, Sergio A. Rodriguez-Aponte, Neil C. Dalvie, Jeong Hyun Lee, Wuhbet Abraham, Diane G. Carnathan, Luis E. Jimenez, Julia T. Ngo, Jason Y. H. Chang, Zeli Zhang, Jingyou Yu, Aiquan Chang, Catherine Nakao, Benjamin Goodwin, Christopher A. Naranjo, Libin Zhang, Murillo Silva, Dan H. Barouch, Guido Silvestri, Shane Crotty, J. Christopher Love, Darrell J. Irvine
Summary: The study suggests that anchoring RBD and molecular adjuvants to alum via phosphate mediation is an effective strategy to enhance the efficacy of SARS-CoV-2 subunit vaccines, significantly boosting humoral immune responses and neutralizing antibody titers.
Article
Immunology
Zheng Wang, Dan Li, Yulu Chen, Yeping Sun, Changzhong Jin, Caiqin Hu, Yi Feng, Junwei Su, Li Ren, Yanling Hao, Shuo Wang, Meiling Zhu, Ying Liu, Jianxun Qi, Biao Zhu, Yiming Shao
Summary: In this study, memory B cells specific to the receptor binding domain (RBD) were isolated from two COVID-19 convalescents, and their antibodies were tested for neutralizing activity against different SARS-CoV-2 variants. Three potent RBD-specific neutralizing antibodies were identified, and the complex structures of two antibodies with RBD were resolved. The analysis of antibody repertoire showed higher CDR3 frequencies in the light chain compared to the heavy chain. This research contributes to the development of RBD-specific antibody-based drugs and immunogens against multiple variants.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kazi Lutful Kabir, Buyong Ma, Ruth Nussinov, Amarda Shehu
Summary: This paper investigates the application of Markov State Models (MSM) in the field of molecular dynamics and explores two important aspects in building the models: the selection of structure representation and the application of clustering algorithms. The results suggest that using fewer dimensions and including more structures leads to better models, which contribute to our understanding of antibody dynamics and antibody-antigen recognition.
Article
Biochemistry & Molecular Biology
Geyan Liu, Kang Wang, Zhen Yang, Xiaoyu Tang, Yungfu Chang, Ke Dai, Xinwei Tang, Bangdi Hu, Yiwen Zhang, Sanjie Cao, Xiaobo Huang, Qigui Yan, Rui Wu, Qin Zhao, Senyan Du, Xintian Wen, Yiping Wen
Summary: In this study, three monoclonal antibodies 5D11, 2H81, and 4F2 against recombinant HbpA were generated. Among them, 5D11 showed strong binding affinity and can potentially be used for serological diagnostic tools for G. parasuis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Mabel T. Y. Wong, Sebastian Kelm, Xiaofeng Liu, Richard D. Taylor, Terry Baker, Jonathan W. Essex
Summary: This study conducted a long-timescale simulation on crystal structures of nine antibody-antigen pairs to explore interface conformation and hydration. Bridging water molecules were found at the antibody-antigen interface. The study also found a correlation between a higher proportion of interactions excluding bulk waters and a lower degree of antigen bound CDR conformational sampling with higher antibody affinity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Danming Tang, Jane Gunson, Eric Tran, Cynthia Lam, Amy Shen, Brad Snedecor, Gavin Barnard, Shahram Misaghi
Summary: Fab antigen binding fragments are a promising class of therapeutics due to their high potency and smaller size compared to full-length antibodies. By increasing heavy chain gene copy numbers in CHO cells, clones with high Fab expression titers can be obtained, suggesting the effectiveness of CHO TI hosts for Fab expression without significant process development efforts.
BIOTECHNOLOGY PROGRESS
(2022)
Article
Chemistry, Analytical
Lidia N. Gomez-Arribas, Augusto Juste-Dolz, Riikka Peltomaa, David Gimenez-Romero, Sergi Morais, Rodrigo Barderas, Carmen Cuadrado, Angel Maquieira, Elena Benito-Pena, Maria C. Moreno-Bondi
Summary: Phage display technology has become a powerful tool for antibody discovery, selecting specific binders for a given antigen from combinatorial libraries. High-affinity VH antibody fragments identified as PHA binders (with apparent association constants ranging from 108 to 1010 M^-1) through QCM-D measurements and PCA, enable evaluation and identification of clone clusters with similar binding characteristics and molecular interaction mechanisms with capabilities beyond conventional techniques.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biotechnology & Applied Microbiology
Worapol Sae-Foo, Gorawit Yusakul, Tharita Kitisripanya, Poomraphie Nuntawong, Seiichi Sakamoto, Waraporn Putalun
Summary: In this study, recombinant Fab antibodies against miroestrol were developed and applied for the quality control of Pueraria candollei. The Fab antibodies showed higher stability and better detection ability compared to ScFv antibodies.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Oncology
Lijun Yao, Julia T. Wang, Reyka G. Jayasinghe, Julie O'Neal, Chia-Feng Tsai, Michael P. Rettig, Yizhe Song, Ruiyang Liu, Yanyan Zhao, Omar M. Ibrahim, Mark A. Fiala, Julie M. Fortier, Siqi Chen, Leah Gehrs, Fernanda Martins Rodrigues, Michael C. Wendl, Daniel Kohnen, Andrew Shinkle, Song Cao, Steven M. Foltz, Daniel Cui Zhou, Erik Storrs, Matthew A. Wyczalkowski, Smrithi Mani, Scott R. Goldsmith, Ying Zhu, Mark Hamilton, Tao Liu, Feng Chen, Ravi Vij, Li Ding, John F. DiPersio
Summary: This study utilizes single-cell transcriptomic profiling to identify potential therapeutic targets for multiple myeloma. The analysis identified 38 marker genes encoding cell-surface and intracellular proteins, including 11 previously uncharacterized targets. The findings provide valuable insights for the development of targeted antitumor therapy.
Article
Fisheries
Karen Manoutcharian, Goar Gevorkian
Summary: The development of recombinant antibody fragments as alternatives to full-length immunoglobulins offers great potential in biomedicine. These antibody fragments have numerous advantages over conventional monoclonal antibodies, such as improved stability and solubility, reduced immunogenicity, higher specificity and affinity, and the ability to target hidden epitopes and cross the blood-brain barrier. Different formats of antibody fragments, like Fab, scFv, sdAbs, and bsAbs, are being evaluated for diagnostics and therapeutics in various studies. This review specifically focuses on VNARs, the smallest recombinant antibody fragment obtained through phage display antibody libraries, and discusses the relevant research up until March 2023.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Virology
Xiangyu Huang, Jingwen Huang, Guihu Yin, Yiqin Cai, Mengli Chen, Jianing Hu, Xiuli Feng
Summary: Avian Influenza (AI) caused by the H9N2 subtype of avian influenza virus (AIV) is a serious threat to both the poultry industry and public health safety. This study successfully cloned the NP gene of the H9N2 strain and generated a monoclonal antibody against the NP protein. The study identified an antigenic epitope on the NP protein that is conserved among multiple AIVs. These findings provide important insights for studying the function of the NP protein and developing a universal assay.
Article
Chemistry, Organic
Mati Gelman, Tlalit Massarano, Ronit Lavi, Gerardo Byk
CURRENT ORGANIC CHEMISTRY
(2018)
Article
Pharmacology & Pharmacy
Thomas Maldiney, Gerardo Byk, Nicolas Wattier, Johanne Seguin, Raz Khandadash, Michel Bessodes, Cyrille Richard, Daniel Scherman
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2012)
Article
Chemistry, Multidisciplinary
Raz Khandadash, Victoria Machtey, Inbal Shainer, Hugo E. Gottlieb, Yoav Gothilf, Yuval Ebenstein, Aryeh Weiss, Gerardo Byk
JOURNAL OF NANOPARTICLE RESEARCH
(2014)
Article
Chemistry, Multidisciplinary
Efrat Shtriker, Sharon Bretler, Anna Munder, Gerardo Byk, Guy Cohen, Michal Kolitz-Domb, Arie Gruzman
JOURNAL OF NANOPARTICLE RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Raz Khandadash, Victoria Machtey, Aryeh Weiss, Gerardo Byk
JOURNAL OF PEPTIDE SCIENCE
(2014)
Article
Biochemistry & Molecular Biology
Hagit Elhawi, Hadar Eini, Amos Douvdevani, Gerardo Byk
Article
Biochemistry & Molecular Biology
Azim Turdiev, Oksana Filiutovich, Fiana Mirkin, Gerardo Byk
JOURNAL OF PEPTIDE SCIENCE
(2019)
Article
Chemistry, Medicinal
Tlalit Massarano, Alexandra Mazir, Ronit Lavi, Gerardo Byk
Article
Chemistry, Multidisciplinary
Sarin Palakkal, Dana Logviniuk, Gerardo Byk
JOURNAL OF NANOPARTICLE RESEARCH
(2020)
Article
Pharmacology & Pharmacy
Lakshmanan Eswaran, Gila Kazimirsky, Gerardo Byk
Summary: The recent application of mRNA protein expression and CRISPR/Cas9 gene editing technology for treating COVID-19 pandemic highlights the urgent need for improved nucleic acid delivery systems, especially for in vivo applications. In this study, novel cross-linked monodispersed nanohydrogels (NHGs) with well-defined sizes ranging between 50-400 nm were designed, synthesized, and characterized. The NHGs were chemically reduced to lead particles with highly positive zeta potential and low cell toxicity. The NHGs form complexes with DNA, fully embedding the DNA inside the NHGs, making them potential tools for gene delivery and foreign protein expression.
Article
Pharmacology & Pharmacy
Lakshmanan Eswaran, Gila Kazimirsky, Ronen Yehuda, Gerardo Byk
Summary: We have developed new formulations of nanohydrogels (NHGs) complexed with DNA devoid of cell toxicity, which can be incubated indefinitely with cells without apparent cellular toxicity and result in sustained expression of foreign proteins for long periods of time. The release of genes from NHGs is time-dependent, suggesting a slow but continuous release of DNA from the particles concomitantly with slow but continuous protein expression. In vivo administration of m-Cherry/NHG complexes also showed delayed but prolonged expression of the marker gene in the tissue of administration.
Meeting Abstract
Biochemistry & Molecular Biology
G. Byk, R. Khandadash, Victoria Machteya, Yuval Ebenstein, Aryeh Weiss
Meeting Abstract
Biochemistry & Molecular Biology
Victoria Machteya, R. Khandadash, Yuval Ebenstein, Aryeh Weiss, G. Byk
Meeting Abstract
Biochemistry & Molecular Biology
G. Byk, V. Machtey, R. Khandadash