期刊
JOURNAL OF IMMUNOLOGY
卷 190, 期 2, 页码 605-612出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1201345
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资金
- Ministry of Education, Science, Sports and Culture of Japan [22689030, 22113007, 23113506]
- Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program) from the Ministry of Education, Science, Sports and Culture of Japan
- Ministry of Health, Labor and Welfare of Japan [H21-010]
- International Human Frontier Science Program [CDA-00059/2009, RGY0077/2011]
- Takeda Science Foundation
- Cell Science Research Foundation
- Astellas Foundation for Research on Metabolic Disorders
- Nakajima Foundation
- Sumitomo Science Foundation
- Grants-in-Aid for Scientific Research [22689030, 24111007, 21390505, 12J10335, 22113007] Funding Source: KAKEN
Osteoclasts are bone-resorbing polykaryons differentiated from monocyte/macrophage-lineage hematopoietic precursors. It remains unclear whether osteoclasts originate from circulating blood monocytes or from bone tissue-resident precursors. To address this question, we combined two different experimental procedures: 1) shared blood circulation parabiosis with fluorescently labeled osteoclast precursors, and 2) photoconversion-based cell tracking with a Kikume Green-Red protein (KikGR). In parabiosis, CX(3)CR1-EGFP knock-in mice in which osteoclast precursors were labeled with EGFP were surgically connected with wild-type mice to establish a shared circulation. Mature EGFP(+) osteoclasts were found in the bones of the wild-type mice, indicating the mobilization of EGFP(+) osteoclast precursors into bones from systemic circulation. Receptor activator for NF-kappa B ligand stimulation increased the number of EGFP(+) osteoclasts in wild-type mice, suggesting that this mobilization depends on the bone resorption state. Additionally, KikGR(+) monocytes (including osteoclast precursors) in the spleen were exposed to violet light, and 2 d later we detected photoconverted red KikGR(+) osteoclasts along the bone surfaces. These results indicate that circulating monocytes from the spleen entered the bone spaces and differentiated into mature osteoclasts during a certain period. The current study used fluorescence-based methods clearly to demonstrate that osteoclasts can be generated from circulating monocytes once they home to bone tissues. The Journal of Immunology, 2013, 190: 605-612.
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