Article
Biochemistry & Molecular Biology
Kavita Reginald, Fook Tim Chew
Summary: This review evaluates the current modes of allergen-specific immunotherapy for cockroach allergens in terms of clinical outcomes and explores future trends in research and development for a more targeted approach to cockroach immunotherapy with better efficacy and fewer adverse effects.
MOLECULAR IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anna Pomes, L. Karla Arruda
Summary: The identification of cockroach allergens, the determination of their function and structure, and the development of molecules with reduced IgE reactivity and T cell modulatory capacity have contributed to understanding cockroach allergy and defining future directions for diagnosis and immunotherapy. Recent advancements include component analyses of B and T cell reactivity and a cockroach immunotherapy trial, CRITICAL.
MOLECULAR IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Neelam Sharma, Sumeet Patiyal, Anjali Dhall, Akshara Pande, Chakit Arora, Gajendra P. S. Raghava
Summary: AlgPred 2.0 is a web server developed for predicting allergenic proteins and regions in a protein, using various approaches and techniques for model training and validation, achieving high prediction accuracy.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Allergy
Maria Suprun, Scott H. Sicherer, Robert A. Wood, Stacie M. Jones, Donald Y. M. Leung, A. Wesley Burks, David Dunkin, Marc Witmer, Galina Grishina, Robert Getts, Mayte Suarez-Farinas, Hugh A. Sampson
Summary: Molecular studies of hen's egg allergens help define allergic phenotypes, with IgE to sequential epitopes on the ovomucoid (OVM) protein associated with a persistent disease. This study constructed an epitope library spanning across 7 allergens and evaluated sequential epitope-specific IgE and IgG(4) among baked-egg reactive or tolerant children. Ses-IgE was identified as a potential biomarker of baked-egg reactivity.
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael F. Sharp, Aya C. Taki, Thimo Ruethers, Juan N. Stephen, Norelle L. Daly, Andreas L. Lopata, Sandip D. Kamath
Summary: This study identified two specific IgE epitopes on parvalbumin from Asian seabass, with lower and patient-specific IgG(4) binding. It highlights the importance of advancing epitope analysis for diagnostics and immunotherapies for fish allergy across all age groups.
MOLECULAR IMMUNOLOGY
(2021)
Review
Allergy
Scott A. Smith, Maksymilian Chruszcz, Martin D. Chapman, Anna Pomes
Summary: In this review, a major milestone in allergy research is discussed, which is the determination of the atomic resolution structure of an authentic human IgE epitope. The molecular insights provided by IgE epitopes will enable the development of structure-based approaches for novel diagnostics, antibody therapeutics, and immunotherapies.
CURRENT ALLERGY AND ASTHMA REPORTS
(2022)
Article
Physiology
Michael E. Scharf, Zachery M. Wolfe, Kapil R. Raje, Mahsa Fardisi, Jyothi Thimmapuram, Ketaki Bhide, Ameya D. Gondhalekar
Summary: Cockroaches, important urban pests, easily develop resistance to insecticides. This research aimed to understand their physiological responses to insecticide selection pressures. The study found that resistant cockroach strains had upregulated detoxification enzyme genes and downregulated genes from microbial and viral origins, suggesting a connection between resistance selection and elimination of commensal microbes.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Masatoshi Nakatsuji, Keisuke Sugiura, Keisuke Suda, Michiko Sakurai, Miki Ubatani, Haruka Muroya, Rina Okubo, Ryo Noguchi, Yoichi Kamata, Yuma Fukutomi, Osamu Ishibashi, Shigenori Nishimura, Takashi Inui
Summary: The study successfully predicted five critical residues that may serve as IgE epitopes of Can f 1, and validated their importance by generating mutants.
Review
Allergy
Luis Caraballo, Kevin Llinas-Caballero
Summary: Helminth infections can either decrease or increase the symptoms of allergic diseases. Certain components from helminths can increase allergic responses and symptoms, despite the immunosuppression caused by the infections. The role of individual IgE-binding molecules in this process is still unclear.
CURRENT ALLERGY AND ASTHMA REPORTS
(2023)
Article
Agriculture, Multidisciplinary
Xin-Rong He, Yang Yang, Ye-Xin Chen, Shuai Kang, Fa-Jie Li, Dong-Xiao Li, Qing-Mei Liu, Gui-Xia Chen, Xiao-Mei Chen, Guang-Ming Liu
Summary: This study investigated the IgE epitope and structure-allergenicity relationship of Scy p 9. A hydrophobic bond was found to be important for maintaining the conformation of Scy p 9. Mutagenesis experiments revealed that key amino acid mutations reduced the IgE-binding capacity and surface hydrophobicity of the mutants. Ten linear epitopes were identified, with L-AA(187-205) showing the strongest IgE binding. IgE epitopes were mapped on the protruding surface of the Scy p 9 tertiary structure and were highly conserved among filamin genes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Fan Yang, Xin Ma, Wei Hu, Ziyi Xiong, Meijiang Huang, Yong Wu, Xuanyi Meng, Zhihua Wu, Anshu Yang, Xin Li, Hongbing Chen
Summary: This study investigates the digestion and transportation properties of three commercial dairy products and the residual allergen epitopes that enter the gastrointestinal mucosa. The results show that dairy processing can affect the digestion and transport characteristics of milk proteins and alter the release of epitope peptides.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Immunology
Anna Pomes, Veronique Schulten, Jill Glesner, Ricardo da Silva Antunes, Aaron Sutherland, Leonard B. Bacharier, Avraham Beigelman, Paula Busse, April Frazier, Alessandro Sette
Summary: In cockroach sensitized patients, the patterns of cockroach allergen-specific IgE and T cell reactivity are unique per subject, lacking immunodominant allergens and correlation with clinical phenotype/disease severity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Weiyuan Hou, Juanjuan Xin, Hui Lu
Summary: This study investigated the resistance development of German cockroach to chlorpyrifos (CPF) over 23 generations, revealing a gradual increase in resistance over time. The resistance evolution showed a pattern of slow growth in the beginning, followed by a period of rapid increase, and then a slowdown towards the later generations. This information could be valuable for guiding the rational use of insecticides to delay resistance development.
SCIENTIFIC REPORTS
(2021)
Article
Allergy
Niki Mitselou, Niklas Andersson, Anna Bergstrom, Inger Kull, Antonios Georgelis, Marianne van Hage, Anna M. Hedman, Catarina Almqvist, Jonas F. Ludvigsson, Erik Melen
Summary: The study found an inverse association between preterm birth and long-term IgE sensitization to common food and inhalant allergens. No relation was found between post-term birth and IgE sensitization. Replication analyses showed similar risk estimates for sensitization in a meta-analysis.
Article
Nutrition & Dietetics
Meijia Huang, Fan Yang, Yong Wu, Xuanyi Meng, Linbo Shi, Hongbing Chen, Xin Li
Summary: This study examined the IgE-binding capacity of main allergens in different yogurts and analyzed the epitopes of major allergen peptides in yogurt. The results showed that major allergens were degraded in yogurts, reducing their IgE and IgG binding ability. The study also identified peptides in yogurt with health benefits, while some IgE and T cell epitopes were destroyed, potentially reducing IgE-binding capacity and inducing oral tolerance.
FRONTIERS IN NUTRITION
(2023)
Article
Allergy
Ning Weng, Marina Miller, Alexa K. Pham, Alexis C. Komor, David H. Broide
Summary: Genetic association studies have shown a strong link between SNP rs12603332 and asthma development. Functional analysis revealed that this SNP can regulate the expression of ORMDL3 and the asthma-related gene ATF6 alpha. The study also demonstrated the use of Cytosine Base Editor technology in investigating asthma-associated SNPs in primary human CD4 cells.
Article
Genetics & Heredity
Nicole F. Brackett, Brian W. Davis, Mazhar Adli, Anna Pomes, Martin D. Chapman
Summary: This study presents a bioinformatics analysis of the major cat allergen, Fel d 1, and demonstrates proof of principle for CRISPR gene editing of the allergen. Using CRISPR-Cas9, the domestic cat Fel d 1 was successfully knocked out in vitro, suggesting that Fel d 1 is a rational and viable candidate for gene deletion.
Article
Allergy
Marilyn Urrutia-Pereira, Herberto Jose Chong-Neto, Isabella Annesi Maesano, Ignacio J. Ansotegui, Luis Caraballo, Lorenzo Cecchi, Carmen Galan, Juan Felipe Lopez, Margarita Murrieta Aguttes, David Peden, Anna Pomes, Josefina Zakzuk, Nelson A. Rosario Filho, Gennaro D'Amato
Summary: The association between asthma and COVID-19 remains contradictory, but type 2 inflammation may reduce the severity of COVID-19. Further research is needed to determine whether asthma diagnosis is a risk factor for severe COVID-19, especially for those with severe disease or non-allergic phenotypes. COVID-19 does not appear to worsen asthma symptoms, and asthma medications should be continued for patients with COVID-19 exposure. Air pollution has direct effects on the properties of pollen grains, which may partially explain its impact on COVID-19. The behavior changes during the COVID-19 pandemic, such as indoor isolation and reduced exposure to outdoor allergens, may have improved certain allergies while exacerbating others.
WORLD ALLERGY ORGANIZATION JOURNAL
(2022)
Review
Allergy
Scott A. Smith, Maksymilian Chruszcz, Martin D. Chapman, Anna Pomes
Summary: In this review, a major milestone in allergy research is discussed, which is the determination of the atomic resolution structure of an authentic human IgE epitope. The molecular insights provided by IgE epitopes will enable the development of structure-based approaches for novel diagnostics, antibody therapeutics, and immunotherapies.
CURRENT ALLERGY AND ASTHMA REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Anna Pomes, L. Karla Arruda
Summary: The identification of cockroach allergens, the determination of their function and structure, and the development of molecules with reduced IgE reactivity and T cell modulatory capacity have contributed to understanding cockroach allergy and defining future directions for diagnosis and immunotherapy. Recent advancements include component analyses of B and T cell reactivity and a cockroach immunotherapy trial, CRITICAL.
MOLECULAR IMMUNOLOGY
(2023)
Article
Allergy
Stephanie Dramburg, Christiane Hilger, Alexandra F. Santos, Leticia de las Vecillas, Rob C. Aalberse, Nathalie Acevedo, Lorenz Aglas, Friedrich Altmann, Karla L. Arruda, Riccardo Asero, Barbara Ballmer-Weber, Domingo Barber, Kirsten Beyer, Tilo Biedermann, Maria Beatrice Bilo, Simon Blank, Philipp P. Bosshard, Heimo Breiteneder, Helen A. Brough, Merima Bublin, Dianne Campbell, Luis Caraballo, Jean Christoph Caubet, Giorgio Celi, Martin D. Chapman, Maksymilian Chruszcz, Adnan Custovic, Rebecca Czolk, Janet Davies, Nikolaos Douladiris, Bernadette Eberlein, Motohiro Ebisawa, Anna Ehlers, Philippe Eigenmann, Gabriele Gadermaier, Mattia Giovannini, Francisca Gomez, Rebecca Grohman, Carole Guillet, Christine Hafner, Robert G. Hamilton, Michael Hauser, Thomas Hawranek, Hans Juergen Hoffmann, Thomas Holzhauser, Tomona Iizuka, Alain Jacquet, Thilo Jakob, Bente Janssen-Weets, Uta Jappe, Marek Jutel, Tanja Kalic, Sandip Kamath, Sabine Kespohl, Joerg Kleine-Tebbe, Edward Knol, Andre Knulst, Jon R. Konradsen, Peter Korosec, Annette Kuehn, Gideon Lack, Thuy-My Le, Andreas Lopata, Olga Luengo, Mika Maekelae, Alessandro Maria Marra, Clare Mills, Martine Morisset, Antonella Muraro, Anna Nowak-Wegrzyn, Roni Nugraha, Markus Ollert, Kati Palosuo, Elide Anna Pastorello, Sarita Ulhas Patil, Thomas Platts-Mills, Anna Pomes, Pascal Poncet, Ekaterina Potapova, Lars K. Poulsen, Christian Radauer, Suzana Radulovic, Monika Raulf, Pierre Rouge, Joaquin Sastre, Sakura Sato, Enrico Scala, Johannes M. Schmid, Peter Schmid-Grendelmeier, Denise Schrama, Helene Senechal, Claudia Traidl-Hoffmann, Marcela Valverde-Monge, Marianne van Hage, Ronald van Ree, Kitty Verhoeckx, Stefan Vieths, Magnus Wickman, Josefina Zakzuk, Paolo M. Matricardi, Karin Hoffmann-Sommergruber
Summary: Since the discovery of IgE as a mediator of allergic diseases, our knowledge about its immunological mechanisms has greatly increased. Innovations in bioscience and technology have facilitated the production of well-defined molecules for component-resolved diagnosis, allowing personalized management of allergic diseases. The Molecular Allergology User's Guide 2.0 provides updated information on allergen molecules, their clinical relevance, and diagnostic algorithms, serving as a valuable resource for clinicians and scientists.
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2023)
Article
Immunology
Glorismer Pena-Castellanos, Bryan R. E. Smith, Anna Pomes, Scott A. Smith, Maria A. Stigler, Hannah L. Widauer, Serge A. Versteeg, Ronald van Ree, Martin D. Chapman, Lorenz Aglas
Summary: This study investigated the biological activity of hIgE mAb derived from allergic donors on three allergens. The hIgE mAb showed effective induction of mediator release, high allergen specificity, and potential for standardization and mechanistic studies of IgE-mediated allergic diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Letter
Allergy
Cosby A. Stone Jr, Jonathan A. Hemler, Stephanie Filep, R. N. Karen Braden, Anna Pomes, Martin D. Chapman, Scott A. Smith
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Article
Medicine, Research & Experimental
Nicole A. LaHood, Jungki Min, Tarun Keswani, Crystal M. Richardson, Kwasi Amoako, Jingjia Zhou, Orlee Marini-Rapoport, Herve Bernard, Stephane Hazebrouck, Wayne G. Shreffler, J. Christopher Love, Anna Pomes, Lars C. Pedersen, Geoffrey A. Mueller, Sarita U. Patil
Summary: Oral immunotherapy (OIT) treats food allergies by gradually increasing oral allergen exposure. However, it only induces sustained clinical tolerance in a subset of patients. By studying the allergen-specific antibodies from OIT patients with sustained and transient responses, researchers identified specific tolerance-associated epitopes recognized by neutralizing antibodies. These antibodies effectively neutralize the allergen and prevent allergic reactions. This study provides insights into antibody-mediated protection in food allergies and opens possibilities for treatments using neutralizing antibodies and hypoallergens.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Allergy
Jungki Min, Alexander C. Y. Foo, Scott A. Gabel, Lalith Perera, Eugene F. DeRose, Anna Pomes, Lars C. Pedersen, Geoffrey A. Mueller
Summary: This study presents crystal structures, NMR, molecular dynamics, and fluorescence studies of dog allergen Can f 1 and its closely related cat allergen Fel d 7. It is found that Can f 1 and Fel d 7 have open and positively charged calyxes, while other dog allergens have closed and negatively charged ones. Fatty acid screening reveals that Can f 1 and Fel d 7 bind multiple ligands with preferences for palmitic acid and oleic acid. The results provide valuable insights into selectivity determinants and candidate ligands for pet allergens Can f 1 and Fel d 7.
FRONTIERS IN ALLERGY
(2023)
Article
Allergy
Bryan R. E. Smith, Kristina Reid Black, Max Bermingham, Sayeh Agah, Jill Glesner, Serge A. Versteeg, Ronald van Ree, Glorismer Pena-Amelunxen, Lorenz Aglas, Scott A. Smith, Anna Pomes, Martin D. Chapman
Summary: Unique human IgE monoclonal antibodies were produced to major inhaled allergens and food allergens, which have potential applications in allergy diagnosis, allergen standardization, and identification of allergenic epitopes for the development of allergy therapeutics. These antibodies were derived from allergic patients and demonstrated high levels of IgE with specificity for various allergens.
FRONTIERS IN ALLERGY
(2023)
Article
Multidisciplinary Sciences
Kriti Khatri, Crystal M. Richardson, Jill Glesner, Anyway Brenda Kapingidza, Geoffrey A. Mueller, Jian Zhang, Cole Dolamore, Lisa D. Vailes, Sabina Wunschmann, R. Stokes Peebles, Martin D. Chapman, Scott A. Smith, Maksymilian Chruszcz, Anna Pomes
Summary: Immunoglobulin E (IgE) antibody plays a critical role in adaptive allergen-induced immune responses, affecting up to 40% of the global population. This study reports the crystal structure of a house dust mite allergen, Der p 2, in complex with a human IgE monoclonal antibody, providing insights into the IgE antibody repertoire to a major mite allergen.
Meeting Abstract
Allergy
Thomas Platts-Mills, Uta Jappe, Christiane Hilger, Marianne van Hage, Gabriele Gadermaier, Edzard Spillner, Jonas Lidholm, Behnam Keshavarz, Rob Aalberse, Ronald Van Ree, Richard Goodman, Anna Pomes
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Meeting Abstract
Allergy
Anna Pomes, Kriti Khatri, Crystal Richardson, Jill Glesner, Anyway Kapingidza, Lisa Vailes, Sabina Wuenschmann, Jian Zhang, R. Stokes Peebles, Martin Chapman, Scott Smith, Maksymilian Chruszcz
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Review
Allergy
Nicole F. Brackett, Anna Pomes, Martin D. Chapman
Summary: Genome engineering using CRISPR technology has the potential to delete allergen genes with high efficiency and precision. Recent studies have shown promise in using CRISPR editing to tackle allergies, particularly in editing the cat allergen Fel d 1.
FRONTIERS IN ALLERGY
(2022)