Article
Immunology
Nadeem Akhter, Shihab Kochumon, Amal Hasan, Ajit Wilson, Rasheeba Nizam, Ashraf Al Madhoun, Fatema Al-Rashed, Hossein Arefanian, Fawaz Alzaid, Sardar Sindhu, Fahd Al-Mulla, Rasheed Ahmad
Summary: The co-stimulation of IFN-gamma and LPS can enhance the expression of CCL2 through the acetylation of H3K27, leading to augmented inflammation in obesity. These findings contribute to a better understanding of the regulatory mechanisms of CCL2 in the pathogenesis of obesity.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Oncology
John M. Fenimore, Danielle A. Springer, Maria E. Romero, Elijah F. Edmondson, Dan W. McVicar, Sudhirkumar Yanpallewar, Michael Sanford, Thea Spindel, Elizabeth Engle, Thomas J. Meyer, Julio C. Valencia, Howard A. Young
Summary: The effect of cytokines on non-traditional immunological targets under conditions of chronic inflammation is explored. Fatigue is associated with autoimmune diseases, and immune dysfunction-driven changes in myocyte mitochondria are hypothesized to play a critical role in fatigue-related pathogenesis. The study shows that persistent low-level expression of IFN-gamma results in mitochondrial and metabolic deficiencies in myocytes, particularly affecting heart function under stress. These findings highlight the actions of IFN-gamma in fatigue, energy metabolism, and autoimmunity.
JOURNAL OF PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Natasa Todorovic-Rakovic
Summary: The evolution of malignant cells is driven by the increasing oncogenic fitness in active interaction with the tumor microenvironment. Mechanisms promoting the adaptive evolution of cancer cells involve clonal selection and selective pressure from host defensive strategies. The immune system, as part of host defense mechanisms, plays a crucial role in modulating cancer evolution and progression.
Article
Fisheries
Qiuju Liu, Menglu Zhang, Jie Wang, Jianjian Zhang, Zhaofei Wang, Jingjiao Ma, Yaxian Yan, Jianhe Sun, Yuqiang Cheng
Summary: In this study, the first bat IRF1 gene was cloned, and its functions in IFN induction were preliminarily identified.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Meiyan Zhong, Yuanting Huang, Bo Zeng, Lihui Xu, Chunsu Zhong, Jiahao Qiu, Xunjia Ye, Mingye Chen, Bo Hu, Dongyun Ouyang, Xianhui He
Summary: The study demonstrates that gossypol can simultaneously activate multiple subroutines of regulated necrosis in macrophages, depending on the action of apoptotic and inflammatory caspases.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Immunology
Caroline Andrews, Mairi H. McLean, Julie A. Hixon, Sergio M. Pontejo, Tregei Starr, Courtney Malo, Margaret Cam, Lisa Ridnour, Heather Hickman, Olivia Steele-Mortimer, David A. Wink, Howard A. Young, Daniel W. McVicar, Wenqing Li, Scott K. Durum
Summary: Mucosal delivery of IL-27 has been shown to have therapeutic benefit in murine models of inflammatory bowel disease (IBD). However, murine colonoids and primary intact colonic crypts were unresponsive to IL-27 in vitro and lacked detectable IL-27 receptors. On the other hand, macrophages in inflamed colon tissue were responsive to IL-27 in vitro and had potent effects on colonic epithelium.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Jiajie Li, Lin Liu, Junmin Xing, Dianhui Chen, Chao Fang, Feng Mo, Yumei Gong, Zhengrong Tan, Guikuan Liang, Wei Xiao, Shanni Tang, Haixia Wei, Shan Zhao, Hongyan Xie, Xingfei Pan, Xiaomao Yin, Jun Huang
Summary: Splenomegaly is a common symptom of malaria, but its causes are not fully understood. In this study, the researchers found that TLR7 played a role in promoting extramedullary splenic erythropoiesis in mice infected with Plasmodium yoelii NSM. They also found that TLR7 enhanced the production of IFN-? which promoted phagocytosis of infected erythrocytes and the iron metabolism of macrophages. Furthermore, neutralizing IFN-? partially inhibited extramedullary splenic erythropoiesis and reduced iron accumulation in the spleen. Overall, this study highlights the importance of TLR7 in regulating splenic erythropoiesis in malaria.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Amelia Barilli, Rossana Visigalli, Francesca Ferrari, Giulia Recchia Luciani, Maurizio Soli, Valeria Dall'Asta, Bianca Maria Rotoli
Summary: This study investigates the molecular pathogenesis of COVID-19-associated ARDS (CARDS) and suggests that the IFN-gamma-IRF-1 signaling pathway may play a role in the observed alveolar epithelial damage. Changes in cell proliferation, autophagy, and protein degradation were observed in alveolar epithelial A549 cells treated with cytokine-enriched conditioned medium from S1-activated macrophages. The addition of the JAK-STAT inhibitor baricitinib prevented these effects.
Article
Veterinary Sciences
Zhenyu Lin, Jie Wang, Nian Zhang, Jianshu Yi, Zhaofei Wang, Jingjiao Ma, Hengan Wang, Yaxian Yan, Kun Qian, Jianhe Sun, Yuqiang Cheng
Summary: This study cloned goose IRF1 (GoIRF1) and conducted bioinformatics analyses to compare its protein homology with IRF1 in other species. The results showed that GoIRF1 induces the expression of interferons, proinflammatory cytokines, and ISGs in geese, playing an important role in antiviral innate immunity.
VETERINARY RESEARCH
(2022)
Article
Cell Biology
Taylor Schmit, Kai Guo, Jitendra Kumar Tripathi, Zhihan Wang, Brett McGregor, Mitch Klomp, Ganesh Ambigapathy, Ramkumar Mathur, Junguk Hur, Michael Pichichero, Jay Kolls, M. Nadeem Khan
Summary: This study identifies IFN-gamma-regulated CCR2(+) monocytes as the main driver of lung damage during IAV infection. IFN-gamma regulates the recruitment and inflammatory phenotype of CCR2(+) monocytes. CD8+ T cells are the main source of IFN-gamma in the infected lungs.
Article
Pharmacology & Pharmacy
Yunlian Zhou, Mengyao Li, Tingyi Lv, Meixia Huang, Beilei Cheng, Yuanyuan Zhang, Jie Zhu
Summary: Type I interferon (IFN-I) plays a critical role in the antiviral immune response, and treatment with Gastrodin (GTD) can increase the survival rate of mice infected with VSV or HSV-1 by promoting the production of IFN-I in macrophages, thereby inhibiting virus infection.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Caio C. B. Bomfim, Logan Fisher, Eduardo P. Amaral, Lara Mittereder, Katelyn McCann, Andre A. S. Correa, Sivaranjani Namasivayam, Muthulekha Swamydas, Mahtab Moayeri, Jonathan M. Weiss, Raj Chari, Daniel W. McVicar, Diego L. Costa, Maria D'Imperio R. Lima, Alan Sher
Summary: This study investigates the pattern recognition receptors and signaling pathways involved in Mycobacterium tuberculosis (Mtb) induced Irg1 gene expression. The findings suggest that TLR-2, MyD88 and NF kappa B signaling play a partial role in Irg1 induction by Mtb. Furthermore, phagocytosis and endosomal acidification are major requirements for Mtb induced Irg1 expression, and the presence of the bacterial ESX-1 secretion system, as well as host STING and Type I IFN receptor (IFNAR) signaling are crucial for the Mtb-induced Irg1 response, while Type II IFN signaling has minimal effect.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Lang Jiang, Lu Liu, Miaomiao Zhang, Linxia Zhang, Cuisong Zhu, Qian He, Lilin Ye, Chen Zhao, Zejun Li, Jianqing Xu, Xiaoyan Zhang
Summary: Research has shown that CD8+ lung tissue resident memory T (T-RM) cells play a crucial role in protecting against respiratory viruses. By studying mouse infection models, it was found that lung CD8+ T-RM cells can quickly respond to antigen re-exposure and induce interferon-stimulated genes (ISGs) in the surrounding tissues. The study also revealed that the induction of certain important antiviral ISGs and the suppression of key inflammatory cytokines are dependent on IFN-gamma signaling.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Baoming Xie, Mingqiu Zhao, Dan Song, Keke Wu, Lin Yi, Wenhui Li, Xiaoming Li, Kun Wang, Jinding Chen
Summary: The study revealed that CSFV infection inhibits the phosphorylation of MTOR leading to autophagy initiation. The MAPK/ERK signaling pathway is involved in CSFV-induced autophagy. CSFV non-structural protein NS5A induced autophagy through the CAMKK2-PRKAA-MTOR signaling pathway.
Article
Immunology
Rajkumar Venkatadri, Vikram Sabapathy, Murat Dogan, Saleh Mohammad, Scott E. Harvey, Sean R. Simpson, Jason M. Grayson, Nan Yan, Fred W. Perrino, Rahul Sharma
Summary: The study reveals a Th1 bias and elevated Tfh cells and germinal center B cells in TREX1 D18N mice. Inhibiting Bcl6 can attenuate Tfh, germinal center, and Th1 responses and rescue TREX1 D18N mice from autoimmunity. These findings suggest that Tfh cells and germinal center B cells may serve as potential therapeutic targets for autoimmune diseases.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Review
Cell Biology
Carolina Soler-Botija, Sonia Vanina Forcales, Antoni Bayes Genis
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2020)
Editorial Material
Cell Biology
Sonia V. Forcales, Montserrat Corominas
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2020)
Article
Dermatology
Carmen De Jesus-Gil, Lidia Sans-de San Nicolas, Ester Ruiz-Romeu, Marta Ferran, Laura Soria-Martinez, Anca Chiriac, Antonio Celada, Ramon M. Pujol, Luis F. Santamaria-Babi
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2020)
Article
Immunology
Lorena Valverde-Estrella, Marti Lopez-Serrat, Guillem Sanchez-Sanchez, Tania Vico, Jorge Lloberas, Antonio Celada
EUROPEAN JOURNAL OF IMMUNOLOGY
(2020)
Article
Biochemical Research Methods
Gabrijela Dumbovic, Xavier Sanjuan, Manuel Perucho, Sonia- Forcales
Summary: Super resolution microscopy and FISH techniques were used to visualize the precise location and interactions of a novel lncRNA TNBL in the nucleus, providing insights into its functionality. Optimized protocols can be employed for single-cell imaging of complex nuclear RNA-protein structures, aiding in understanding spatial arrangements within cells.
Article
Cell Biology
Juan Tur, Selma Pereira-Lopes, Tania Vico, Eros A. Marin, Juan P. Munoz, Maribel Hernandez-Alvarez, Pere-Joan Cardona, Antonio Zorzano, Jorge Lloberas, Antonio Celada
Article
Immunology
Juan Tur, Consol Farrera, Ester Sanchez-Tillo, Tania Vico, Paula Guerrero-Gonzalez, Ainhoa Fernandez-Elorduy, Jorge Lloberas, Antonio Celada
Summary: The induction of MHC class II proteins by IFN-γ in macrophages requires the involvement of signaling pathways, with CypA playing a key role in the process by affecting the expression of CIIta. This study highlights the importance of cross talk between different pathways, with STAT1 being essential for the two-step induction of CIIta.
Article
Multidisciplinary Sciences
Neus Sole-Morata, Isabel Baenas, Mikel Etxandi, Roser Granero, Sonia Forcales, Manel Gene, Carme Barrot, Monica Gomez-Pena, Jose M. Menchon, Nicolas Ramoz, Philip Gorwood, Fernando Fernandez-Aranda, Susana Jimenez-Murcia
Summary: This study assessed the involvement of genetic factors, specifically neurotrophin (NTF) genes, in the development of gambling disorder (GD). The findings suggest a potential link between certain NTF gene variants and GD, supporting the role of the NTF family in the pathogenesis of GD. Alterations in the cross-regulation of NTF signaling pathways may contribute to the biological vulnerability for GD.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Tania Vico, Catrin Youssif, Fathema Zare, Monica Comalada, Carlos Sebastian, Jorge Lloberas, Antonio Celada
Summary: In this study, it was found that GM-CSF provides better protection against DNA damage and promotes faster DNA repair in macrophages compared to M-CSF. The presence of M-CSF resulted in a higher number of apoptotic cells after DNA damage. The protective effect of GM-CSF against DNA damage is associated with its induction of differentiation markers and pro-survival protein.
Article
Cell Biology
Luis Arpa, Carlos Batlle, Peijin Jiang, Carme Caelles, Jorge Lloberas, Antonio Celada
Summary: IL-4 is a key activator of macrophage alternative activation and induces an anti-inflammatory phenotype. The IL-4 signaling pathway involves STAT-6 and MAPK family members. We found that JNK-1 activation regulates the expression of genes involved in alternative activation in response to IL-4.
Article
Pharmacology & Pharmacy
Cristina Moreta-Moraleda, Cristina Queralt, Carla Vendrell-Ayats, Sonia Forcales, Eva Martinez-Balibrea
Summary: Colorectal cancer is the third most prevalent cancer globally and chemoresistance is the main cause of treatment failure. Epigenetic factors may serve as clinical biomarkers for colorectal cancer. In recent years, studies have investigated the relationship between chemotherapeutic treatment response and DNA methylation, histone modifications, and chromatin remodelers, but their exact relationship in colorectal cancer is still unclear.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Stefano Zoroddu, Luca Sanna, Valentina Bordoni, Weidong Lyu, Gabriele Murineddu, Gerard A. Pinna, Sonia Vanina Forcales, Arturo Sala, David J. Kelvin, Luigi Bagella
Summary: This study investigated the characterization of two new compounds 6 and 14 on HeLa and PC-3 cancer cell lines. The results showed that 6 and 14 strongly inhibited the proliferation of cancer cells by arresting them in the G2/M phase of the cell cycle. Transcriptome analysis showed that exposure of HeLa and PC-3 cells to the compounds caused a marked reprogramming of gene expression. Functional enrichment analysis indicated that differentially expressed genes were significantly enriched throughout the cell cycle and cancer-related biological processes. Furthermore, PPI network, hub gene, and CMap analyses revealed that compounds 14 and 6 shared target genes with established microtubule inhibitors, indicating points of similarity between the two molecules and microtubule inhibitors in terms of the mechanism of action. They were also able to influence the polymerization process of tubulin, suggesting the potential of these new compounds to be used as efficient chemotherapeutic agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)