Review
Chemistry, Multidisciplinary
Yichao Lu, Yingying Shi, Jian You
Summary: Dendritic cells play an important role in the cross presentation of exogenous antigens, contributing to a diversified T-cell activation and efficient anti-tumor immune response. Understanding the mechanisms and regulation strategies of cross presentation by DC populations can lead to better clinical efficacy in antitumor therapy.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Oncology
Tom J. Harryvan, Marten Visser, Linda de Bruin, Leonie Plug, Lisa Griffioen, Arend Mulder, Peter A. van Veelen, Gerbrand J. van Der Heden van Noort, Marlieke Lm Jongsma, Miranda H. Meeuwsen, Emmanuel Jhj Wiertz, Saskia J. Santegoets, James Ch Hardwick, Thorbald Van Hall, Jacques Neefjes, Sjoerd H. Van der Burg, Lukas J. A. C. Hawinkels, Els M. E. Verdegaal
Summary: This study found that human CRC-derived CAFs have an enhanced ability to cross-present antigens, which involves the participation of lysosomal protease cathepsin S. The interaction between cross-presenting CAFs and CD8+ T cells suppresses T cell function.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Immunology
Ray A. Ohara, Kenneth M. Murphy
Summary: Cross-priming, initially recognized in cytotoxic T lymphocyte (CTL) responses, involves the presentation of minor histocompatibility antigens by antigen presenting cells (APCs) derived from immunizing cells. As understanding of T cell receptor antigen recognition progressed, cross-priming was redefined as cross-presentation and expanded to include different forms of antigens and APCs not involved in in vivo CTL priming. In vitro cell models have been utilized for studying cross-presentation, but recent studies have shown differences between these models and in vivo APCs. Current research focuses on validating in vivo pathways and gene candidates for cross-presentation, and evaluating their contributions to CTL responses across different antigens and immunologic settings.
SEMINARS IN IMMUNOLOGY
(2023)
Review
Immunology
Francois-Xavier Mauvais, Peter van Endert
Summary: The critical role of conventional dendritic cells in the immune response to tumors and pathogens is well-established. However, various other cell types, including myeloid, lymphoid, endothelial, epithelial, and stromal cells, have been shown to also possess the ability to cross-present antigens. This review analyzes the literature on this topic, discussing the antigens and readouts used, mechanistic insights, and in vivo experimentation. While many studies rely on artificial systems, there is evidence to suggest that cross-presentation by non-dendritic cells may have significant implications in anti-tumor immunity and autoimmunity.
SEMINARS IN IMMUNOLOGY
(2023)
Article
Immunology
Ligu Mi, Yan Wang, Hui Xu, Yu Wang, Jia Wu, Hui Dai, Yu Zhang
Summary: Prak deficiency impairs various functions of macrophages, including bactericidal activity and ROS production, affecting the clearance of intracellular pathogens in mice.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Lili Zheng, Junqiu Jia, Yan Chen, Renyuan Liu, Runjing Cao, Manlin Duan, Meijuan Zhang, Yun Xu
Summary: The study demonstrated the therapeutic potential of PTX in VAD, alleviating ischemic white matter injury through modulating Mertk-mediated myelin clearance in microglia.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Oncology
Jinyang Hu, Feng Dong, You He, Xianyou Xia, Fangling Cheng, Sui Chen, Xiaoshuang Hou, Po Zhang, Guohao Liu, Ying Li, Qian Gao, Minhai Dong, Ting Li, Wei Li, Qungen Xiao, Xiaopeng Li, Xingjiang Yu, Guifa Xi, Dongsheng Guo, Xudong Wu, Baofeng Wang
Summary: This study reveals that GBM cells with high level LRIG2 can escape phagocytosis by tumor-associated microglia/macrophages, highlighting the potential of early clinical trials targeting LRIG2 and CD47-SIRP alpha as a novel treatment for GBM.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Immunology
Karthik Dhatchinamoorthy, Jeff D. Colbert, Kenneth L. Rock
Summary: Loss of MHC I antigen presentation is common in many cancers, which may impair immune responses and affect the efficacy of immunotherapy. Studies have discussed underlying mechanisms through which some cancers evade immune killing by shutting down the MHC I pathway, and proposed potential strategies to overcome this limitation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Engineering, Environmental
Jiajia Wang, Xiaoya Liu, Yanfang Qi, Zonghua Liu, Yuepeng Cai, Renfeng Dong
Summary: This study describes an effective strategy for delivering intracellular antigens using ultrasound-propelled gold nanowires nanomotors modified with a model antigen. The nanomotors facilitate rapid internalization into antigen-presenting cells, leading to activation and cross-presentation of the cells with significant increase in MHC I and MHC II molecules expression levels. This novel vaccine delivery strategy based on AuNWs nanomotors is expected to enhance cellular immunity and provide a new approach to immunotherapy.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Cell Biology
Jinyu Wang, Michela Manni, Anne Baerenwaldt, Ronja Wieboldt, Nicole Kirchhammer, Robert Ivanek, Michal Stanczak, Alfred Zippelius, David Koenig, Natalia Rodrigues Manutano, Heinz Laeubli
Summary: Interactions between sialylated glycans and Siglec receptors have been identified as a potential new immune checkpoint to improve anticancer immunity. In this study, it was found that cancer-associated dendritic cells (DCs) have a high expression of inhibitory Siglecs, which can impair their maturation states. Furthermore, removing these inhibitory Siglecs from DCs enhances their capability to prime antigen-specific T cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Hongyan Ren, Yongbin Mou, Lin Lin, Lixin Wang, Hongming Hu
Summary: The research found that coating conventional aluminum adjuvants with polyethyleneimine (PEI) could enhance antigen cross-presentation of DCs, providing an alternative approach for the rapid translation of alumina nanoparticles adjuvants into clinical application. PEIs exerted differential effects on antigen cross-presentation, suggesting a potential for improving the efficacy of antigen delivery.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Article
Engineering, Biomedical
Xiaodi Li, Pengfei Yuan, Haiyuan Yang, Xiaoqing Zong, Caiqi Yang, Xinjie Chen, Yuchao Li, Xiaodie Yan, Yaoqi Wen, Tianci Zhu, Qian Zhang, Wei Xue, Jian Dai
Summary: A novel virus-like nanotherapeutic (siRNA@HCM) is developed to enhance the antitumor immune response. It achieves this by improving tumor intrinsic MHC-I antigen presentation and promoting dendritic cell maturation. The use of siRNA@HCM also turns cold tumors into hot tumors and enhances the therapeutic effect of programmed death-1 inhibitor.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Ian Mantel, Barzan A. A. Sadiq, J. Magarian Blander
Summary: This article discusses the importance of the transporter associated with antigen processing (TAP) protein in cellular immune responses, particularly in cross-presentation. TAP plays a crucial role in transporting peptides to the endoplasmic reticulum as well as in phagosomes and endosomes, and its expression and function are often compromised by cancer and viruses.
MOLECULAR IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Carolina Johnstone, Elena Chaves-Pozo
Summary: This review focuses on the essential components of antigen processing and presentation (APP) in teleost fish and the increasing evidence related to the modulation of APP and autophagy during pathogen infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Genetics & Heredity
Evangelos Koustas, Eleni-Myrto Trifylli, Panagiotis Sarantis, Nikolaos Papadopoulos, Konstantinos Papanikolopoulos, Georgios Aloizos, Christos Damaskos, Nikolaos Garmpis, Anna Garmpi, Dimitris Matthaios, Michalis. V. V. Karamouzis
Summary: Autophagy is a crucial process for cell degradation and recycling, which affects cellular functions and is associated with tumorigenesis, tumor-stroma interactions, and resistance to cancer therapy. It influences the tumor microenvironment and plays a key role in immune cell functions, neo-antigen presentation, and cross-presentation. Autophagy is currently an important target in immunotherapy.
Article
Immunology
Shivam K. Purohit, Carolyn Samer, Hamish E. G. McWilliam, Renee Traves, Megan Steain, Brian P. McSharry, Paul R. Kinchington, David C. Tscharke, Jose A. Villadangos, Jamie Rossjohn, Allison Abendroth, Barry Slobedman
Summary: This study demonstrates that varicella zoster virus suppresses the expression of antigen presentation molecule MR1, highlighting the intricate temporal relationship between infection and ligand availability. The study also suggests that VZV likely encodes multiple viral genes targeting MR1.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Marina E. Ivanova, Natalya Lukoyanova, Sony Malhotra, Maya Topf, Joseph A. Trapani, Ilia Voskoboinik, Helen R. Saibil
Summary: Perforin is a protein that forms pores to facilitate the killing of infected or cancerous cells by the immune system. It is released from cytotoxic lymphocytes and binds to target cell membranes, leading to the rapid death of the target cells. The structure and formation process of the perforin pores have been studied, providing insights into their stability and the effects of pathogenic mutations.
Article
Critical Care Medicine
Tanguy Chaumette, Raphael Cinotti, Alice Molle, Pierre Solomon, Louise Castain, Cynthia Fourgeux, Hamish E. G. McWilliam, Barbara Misme-Aucouturier, Alexis Broquet, Cedric Jacqueline, Mickael Vourc'h, Delphine Fradin, Celine Bossard, Laurent David, Emmanuel Montassier, Cecile Braudeau, Regis Josien, Jose A. Villadangos, Karim Asehnoune, Celine Bressollette-Bodin, Jeremie Poschmann, Antoine Roquilly
Summary: Brain injury induces immunosuppression, increasing the risk of viral reactivations and impairing neurological recovery. This study found that lung replication of herpesviridae is associated with poor recovery after brain injury, and a specific monocyte signature can predict the neurological outcome at 6 months.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Biochemical Research Methods
Vanta J. Jameson, Tina Luke, Yuting Yan, Angela Hind, Maximilien Evrard, Kevin Man, Laura K. Mackay, Axel Kallies, Jose A. Villadangos, Hamish E. G. McWilliam, Alexis Perez-Gonzalez
Summary: Understanding the impact of autofluorescence on signal resolution in cytometry is crucial for experimental design and data quality. This study provides mathematical predictions and empirical evidence to support the importance of autofluorescence extraction through full spectrum unmixing. The study also presents a novel method for isolating pure cell subset-specific autofluorescence profiles based on high dimensionality reduction algorithms.
Article
Multidisciplinary Sciences
Haiyin Liu, Kayla R. Wilson, Ashley M. Firth, Christophe Macri, Patrick Schriek, Annabelle B. Blum, Javiera Villar, Samuel Wormald, Mitch Shambrook, Bangyan Xu, Hui Jing Lim, Hamish E. G. McWilliam, Andrew F. Hill, Laura E. Edgington-Mitchell, Irina Caminschi, Mireille H. Lahoud, Elodie Segura, Marco J. Herold, Jose A. Villadangos, Justine D. Mintern
Summary: This study reveals the critical role of ubiquitin-like protein 3 (UBL3) in the trafficking process controlled by MARCH1. UBL3 has wide-ranging immunological consequences and is essential for immune responses.
NATURE COMMUNICATIONS
(2022)
Article
Cardiac & Cardiovascular Systems
Mickael Vourc'h, Antoine Roquilly, Adrien Foucher, Christelle Retiere, Fanny Feuillet, Sapna Devi, Hamish E. G. McWilliam, Cecile Braudeau, Geraldine Bourreille, Abderrahman Hachani, Dermot O'Kane, Scott N. Mueller, Joseph Ischia, Jean-Christian Roussel, Jean-Christophe Rigal, Regis Josien, Bertrand Rozec, Jose A. Villadangos, Karim Asehnoune
Summary: This study found that transfusion of packed red blood cells with high levels of MRP_14 may increase the incidence of AKI after cardiac surgery. Higher concentrations of MRP_14 were found in packed red blood cells from female donors or prepared by whole blood filtration.
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2022)
Article
Veterinary Sciences
Sathya N. Kulappu Arachchige, Gregory J. Underwood, Daniel M. Andrews, A. M. Hasanthi Abeykoon, Nadeeka K. Wawegama, Glenn F. Browning
Summary: This study reviewed the literature on the evaluation of vaccine efficacy against pathological changes caused by M. gallisepticum in poultry. The study found that assessment of tracheal lesions is a more reliable method for evaluating disease induced by M. gallisepticum compared to assessment of air sac lesions. Furthermore, the study highlighted the importance of using a standardized model for evaluation and reporting of M. gallisepticum vaccines.
Article
Cell Biology
Marine Gros, Elodie Segura, Derek C. Rookhuizen, Blandine Baudon, Sandrine Heurtebise-Chretien, Nina Burgdorf, Mathieu Maurin, Eugene A. Kapp, Richard J. Simpson, Patrycja Kozik, Jose A. Villadangos, Mathieu J. M. Bertrand, Marianne Burbage, Sebastian Amigorena
Summary: Despite its importance in immune responses, the molecular pathways underlying antigen cross-presentation are not fully understood. This study reveals that membrane repair plays a crucial role in containing antigen export to the cytosol and cross-presentation in conventional dendritic cells (cDCs).
Article
Cell Biology
Hui Jing Lim, Jacinta M. Wubben, Cristian Pinero Garcia, Sebastian Cruz-Gomez, Jieru Deng, Jeffrey Y. W. Mak, Abderrahman Hachani, Regan J. Anderson, Gavin F. Painter, Jesse Goyette, Shanika L. Amarasinghe, Matthew E. Ritchie, Antoine Roquilly, David P. Fairlie, Katharina Gaus, Jamie Rossjohn, Jose A. Villadangos, Hamish E. G. McWilliam
Summary: MR1 is a conserved microbial immune-detection system in mammals that presents antigens to specific lymphocytes, contributing to host defense and tissue repair. This study reveals that human MR1 interacts with the endocytic adaptor protein 2 (AP2) complex to regulate its internalization from the cell surface, thus controlling antigen presentation and microbial metabolic detection.
JOURNAL OF CELL BIOLOGY
(2022)
Editorial Material
Immunology
Antoine Roquilly, Jose A. Villadangos
Summary: The BCG vaccine provides homotypic protection against tuberculosis and surprising heterotypic protection against other pathogens. New research reveals that BCG vaccination leads to leakage of gut microbial metabolites into circulation, triggering protective changes in alveolar macrophages against pneumonia.
Article
Immunology
Ana Maria Valencia-Hernandez, Thomas Zillinger, Zhengyu Ge, Peck S. Tan, Anton Cozijnsen, Geoffrey I. McFadden, Mireille H. Lahoud, Irina Caminschi, Winfried Barchet, William R. Heath, Daniel Fernandez-Ruiz
Summary: Tissue resident memory T cells (TRM cells) play a crucial role in tissue surveillance and rapid response to infection. This study explores the potential of various adjuvants targeting different pathogen recognition receptors to induce CD8' TRM formation. CpG oligodeoxynucleotides (ODN) were found to be the most efficient inducers of liver TRM cells among all tested adjuvants. Additionally, combining CpG ODN with cationic liposome DOTAP further enhances their potency. This study provides valuable insights for the development of liver TRM-based vaccines.
Article
Immunology
Angelo Ferreira Chora, Sofia Marques, Joana Lisboa Goncalves, Priscila Lima, Daniel Gomes da Costa, Daniel Fernandez-Ruiz, Maria Ines Marreiros, Pedro Ruivo, Tania Carvalho, Ruy M. Ribeiro, Karine Serre, William R. Heath, Bruno Silva-Santos, Ann T. Tate, Maria M. Mota
Summary: During malaria infection, Plasmodium replicates in the liver and later infects red blood cells. It has been commonly believed that liver infection does not affect disease pathology as clinical manifestations only occur during the blood stage. However, a study using a mouse model uncoupling liver and blood stages of infection revealed that signals from both stages determine mortality outcomes. This finding was mediated by the activation of Vy4+ y8 T cells during the liver stage, which subsequently influenced cytokine profiles during the blood stage. The study also suggested that IL-17 produced by y8 T cells played a role in protecting mice from lethal cerebral malaria.
Article
Immunology
Sara M. Klose, David P. De Souza, Jillian F. Disint, Daniel M. Andrews, Gregory J. Underwood, Chris J. Morrow, Marc S. Marenda, Amir H. Noormohammadi
Summary: The live attenuated temperature sensitive vaccine strain MS-H contains 32 single nucleotide polymorphisms (SNPs) compared to the field strain. Three of these SNPs have been shown to be prone to reversion under field conditions. Reisolates with the 86079/7NS genotype in obgE, oppF, and gapdh were more immunogenic and transmissible in chickens. Changes in OppF influenced the uptake of peptides and/or amino acids, while GAPDH played a role in metabolism of glycerophospholipids and the ADI pathway. This study highlights the role of ObgE, OppF, and GAPDH in M. synoviae metabolism and their contribution to the attenuation of MS-H.
Article
Immunology
Caroline L. Ashley, Brian P. McSharry, Hamish E. G. McWilliam, Richard J. Stanton, Ceri A. Fielding, Rommel A. Mathias, David P. Fairlie, James McCluskey, Jose A. Villadangos, Jamie Rossjohn, Allison Abendroth, Barry Slobedman
Summary: This study reveals that human cytomegalovirus (HCMV) inhibits the MR1 pathway and disrupts the MR1:MAIT cell axis through the viral protein gpUS9. The interaction between this virus and MAIT cells in the context of viral infection is not well characterized.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Patrick Schriek, Jose A. Villadangos
Summary: Antigen-presenting cells capture or synthesize antigens and present them on their plasma membrane through major histocompatibility complex (MHC) molecules. Trogocytosis is a mechanism where cells acquire fragments from other cells and incorporate them into their own plasma membrane, including intact antigens and MHC molecules. Trogocytosis expands cellular immunological functions with both beneficial and deleterious consequences.
CURRENT OPINION IN IMMUNOLOGY
(2023)
