Article
Multidisciplinary Sciences
Haiyin Liu, Kayla R. Wilson, Ashley M. Firth, Christophe Macri, Patrick Schriek, Annabelle B. Blum, Javiera Villar, Samuel Wormald, Mitch Shambrook, Bangyan Xu, Hui Jing Lim, Hamish E. G. McWilliam, Andrew F. Hill, Laura E. Edgington-Mitchell, Irina Caminschi, Mireille H. Lahoud, Elodie Segura, Marco J. Herold, Jose A. Villadangos, Justine D. Mintern
Summary: This study reveals the critical role of ubiquitin-like protein 3 (UBL3) in the trafficking process controlled by MARCH1. UBL3 has wide-ranging immunological consequences and is essential for immune responses.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Yanan Wu, Nianzhi Zhang, Keiichiro Hashimoto, Chun Xia, Johannes M. Dijkstra
Summary: The structures of peptide-loaded major histocompatibility complex class I (pMHC-I) and class II (pMHC-II) complexes are similar, but there are differences in the components. Comparative analysis of primitive vertebrate species' pMHC-I and pMHC-II structures sheds light on the evolution of MHC structures. The establishment of pMHC-I involved the development of important new functions that have been well conserved since their early inception.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kenji Sugata, Yukiko Matsunaga, Yuki Yamashita, Munehide Nakatsugawa, Tingxi Guo, Levon Halabelian, Yota Ohashi, Kayoko Saso, Muhammed A. Rahman, Mark Anczurowski, Chung-Hsi Wang, Kenji Murata, Hiroshi Saijo, Yuki Kagoya, Dalam Ly, Brian D. Burt, Marcus O. Butler, Tak W. Mak, Naoto Hirano
Summary: By combining molecular biological and immunological techniques, this study successfully cloned sequences encoding HLA-DP, HLA-DQ, and HLA-DR molecules with enhanced CD4 binding affinity and produced affinity-matured class II dimers that stain antigen-specific T cells better than conventional multimers. These affinity-matured class II dimers will aid in the investigation of human CD4(+) T-cell responses, providing a comprehensive library of dimers for HLA-DP, HLA-DQ, and HLA-DR alleles. The readily detectable CD4(+) T cells with class II MHC dimers are crucial for studying human immune responses.
NATURE BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Binkai Chi, Muhammet M. Oeztuerk, Christina L. Paraggio, Claudia E. Leonard, Maria E. Sanita, Mahtab Dastpak, Jeremy D. O'Connell, Jordan A. Coady, Jiuchun Zhang, Steven P. Gygi, Rodrigo Lopez-Gonzalez, Shanye Yin, Robin Reed
Summary: Mutations in RNA/DNA-binding proteins can cause ALS, but the exact disease mechanisms are still unclear. This study found that a group of ALS-associated proteins can affect the expression of genes involved in the MHC II antigen presentation pathway. Additionally, hematopoietic progenitor cells with mutations also exhibit disrupted MHC II expression. These findings suggest that the loss of the MHC II pathway may result in the immune system's failure to protect motor neurons from ALS-related damage.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Yue Chen, Sirui Li, Xiaoyong Huang, Chenhui Wang, Yue Pan, Qun Xiang, Zeqing Feng, Lei Fei, Yuzhang Wu, Zhihua Ruan, Yunfei An, Yongwen Chen
Summary: Our previous research showed that MS4A6D is an adapter of VSIG4 that controls NLRP3 inflammasome activation. It is restricted to mononuclear phagocytes and its gene transcript is regulated by the transcription factor NKX2-1. Deletion or mutation of MS4A6D alleviates inflammation and prevents endotoxin lethality, suggesting that MS4A6D can be a novel target for treating macrophage-associated disorders.
MOLECULAR IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Ratna S. Wijaya, Scott A. Read, Naomi R. Truong, Shuanglin Han, Dishen Chen, Haleh Shahidipour, Nicole L. Fewings, Stephen Schibeci, Mahmoud K. Azardaryany, Grant P. Parnell, David Booth, David van der Poorten, Rita Lin, Jacob George, Mark W. Douglas, Golo Ahlenstiel
Summary: Research has shown the existence of HBV-specific memory natural killer cells (mNKs) in humans following vaccination against hepatitis B virus (HBV) or during chronic HBV infection. These mNKs exhibit antigen specificity and functional activity, providing opportunities for novel treatments targeting chronic HBV infection.
Article
Biochemistry & Molecular Biology
M. Cortazar-Chinarro, A. Richter-Boix, P. Rodin-Morch, P. Halvarsson, J. B. Logue, A. Laurila, J. Hoglund
Summary: Microbiomes play a crucial role in determining the ecology and behavior of their hosts. This study investigated the effects of geography, evolutionary history, and host genetics on the skin microbiome diversity and structure in a widespread amphibian. The results showed that microbiome diversity was correlated with host genetic diversity, and the bacterial community composition varied between geographical clusters and specific host genetic variations.
Article
Multidisciplinary Sciences
Qiuming Zeng, Na Tang, Yilei Ma, Hui Guo, Yixia Zhao, Rong Tang, Chengkai Yan, Song Ouyang, Wallace Y. Langdon, Huan Yang, Matthew C. O'Brien, Jian Zhang
Summary: This study reveals a previously unappreciated function of Cbl-b in the regulation of pathogenic Th17 responses. Cbl-b expression in macrophages restrains the generation of pathogenic Th17 cells and the development of EAE, while its expression in T cells or dendritic cells has no effect.
Article
Immunology
Angelica W. Y. Lau, Vivian M. Turner, Katherine Bourne, Jana R. Hermes, Tyani D. Chan, Robert Brink
Summary: The BAFF/BAFFR signaling pathway is necessary for the generation of unmutated, GC-independent MBCs derived directly from activated B cells, while not affecting GC B cells or GC-derived MBCs. Both types of MBCs require input from this pathway for optimal long-term survival.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Immunology
Fabrizio Celesti, Andrea Gatta, Mariam Shallak, Anna Maria Chiaravalli, Michele Cerati, Fausto Sessa, Roberto S. Accolla, Greta Forlani
Summary: In an animal experimental system, vaccination with glioblastoma GL261 cells expressing CIITA showed successful protection against glioblastoma growth. The activation of MHC class II through CIITA led to rapid infiltration of CD4+ and CD8+ T cells, resulting in the rejection or retardation of tumor growth. This approach demonstrates the potential application of novel immunotherapeutic strategies in the clinical setting.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Yusuke Kasuga, Ryota Ouda, Masashi Watanabe, Xin Sun, Miki Kimura, Shigetsugu Hatakeyama, Koichi S. Kobayashi
Summary: Major histocompatibility complex (MHC) class I and II molecules are critical for adaptive immunity. The protein FBXO11 has been identified as an E3 ligase that regulates CIITA protein level through ubiquitination-mediated degradation, thus determining the level of MHC-II.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Medical Laboratory Technology
Maria-Luisa Del Rio, Tuan H. Nguyen, Laurent Tesson, Jean-Marie Heslan, Alfonso Gutierrez-Adan, Raul Fernandez-Gonzalez, Julia Gutierrez-Arroyo, Leo Buhler, Jose-Antonio Perez-Simon, Ignacio Anegon, Jose-Ignacio Rodriguez-Barbosa
Summary: CD160 deficiency influences the course of alloreactive cytotoxic responses and the differentiation of effector T cells, but does not significantly affect the survival rate of allogeneic skin grafts. CD160 may serve as an alternative costimulatory molecule for the modulation of alloreactive CD8 T cell responses.
TRANSLATIONAL RESEARCH
(2022)
Article
Biochemical Research Methods
Laura C. Demmers, Wei Wu, Albert J. R. Heck
Summary: HLA molecules play critical roles in the adaptive immune system by presenting small peptides to signal cell health status to the immune system. This study investigated the adaptive response of a B lymphoblastic cell line to high temperature treatment, revealing potential preparations for immune-like responses in the absence of invading pathogenic peptides. The findings suggest intriguing temperature-sensitive adaptations in this particular B cell line.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Gastroenterology & Hepatology
Dalila Mele, Barbara Oliviero, Stefania Mantovani, Serena Ludovisi, Andrea Lombardi, Francesca Genco, Roberto Gulminetti, Stefano Novati, Mario U. Mondelli, Stefania Varchetta
Summary: The study found that adaptive NK cells in patients with chronic HCV infection have specific characteristics before treatment, such as increased PD-1 expression and decreased ADCC activity, which can be improved and restored to normal after treatment. Additionally, hyperfunctional NK cells are affected during HCV infection, and DAA therapy can improve this effect.
Article
Immunology
Nyerhovwo Obarorakpor, Deep Patel, Reni Boyarov, Nansalmaa Amarsaikhan, Joseph Ray Cepeda, Doreen Eastes, Sylvia Robertson, Travis Johnson, Kai Yang, Qizhi Tang, Li Zhang
Summary: In spontaneous type 1 diabetes (T1D) non-obese diabetic (NOD) mice, the insulin B chain peptide 9-23 (B:9-23) can bind to the MHC class II molecule (IA(g7)) in register 3 (R3), creating a bimolecular IA(g7)/InsulinB:9-23 register 3 conformational epitope (InsB:R3). InsB:R3-specific chimeric antigen receptor (CAR) can guide CAR-expressing CD8 T cells to migrate to the islets and pancreatic lymph nodes. Regulatory T cells (Tregs) specific for an islet antigen can suppress autoimmune reactivity in islets and protect against T1D.
FRONTIERS IN IMMUNOLOGY
(2023)