期刊
JOURNAL OF IMMUNOLOGY
卷 186, 期 3, 页码 1421-1431出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1002587
关键词
-
类别
资金
- National Institutes of Health (NIAID) [AI022033]
We evaluated the presentation of blood-derived protein Ags by APCs in the thymus. Two conventional dendritic cells (cDCs), the CD8 alpha(+)Sirp alpha(-)CD11c(hi) (CD8 alpha(+) cDC) and the CD8 alpha(-)Sirp alpha(+)CD11c(hi) (Sirp alpha(+) cDC), were previously identified as presenting MHC class II bound peptides from hen egg white lysozyme (HEL) injected intravenously. All thymic APCs acquired the injected HEL, with the plasmacytoid dendritic cell being the best, followed by the Sirp alpha(+) cDC and the CD8 alpha(+) cDC. Both cDCs induced to similar extent negative selection and regulatory T cells in HEL TCR transgenic mice, indicating a redundant role of the two cDC subsets in the presentation of blood-borne HEL. Immature dendritic cells or plasmacytoid dendritic cells were considerably less efficient. Batf3(-/-) mice, with significantly reduced numbers of CD8 alpha(+) cDCs, were not impaired in HEL presentation by I-A(k) molecules of thymic APCs. Lastly, clodronate liposome treatment of TCR transgenic mice depleted blood APCs including Sirp alpha(+) cDCs without affecting the number of thymic APCs. In such treated mice, there was no effect on negative selection or regulatory T cells in mice when administering HEL, indicating that the T cell responses were mediated primarily by the cDCs localized in the thymus. The Journal of Immunology, 2011, 186: 1421-1431.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据