Article
Microbiology
Mahebali Tabusi, Sigrun Thorsdottir, Maria Lysandrou, Ana Rita Narciso, Melania Minoia, Chinmaya Venugopal Srambickal, Jerker Widengren, Birgitta Henriques-Normark, Federico Iovino
Summary: Neuronal damage is a major consequence of bacterial meningitis, with Streptococcus pneumoniae playing a prominent role in causing neurological sequelae. This study investigated mechanisms by which pneumococci interact with neurons, leading to neuronal death. The findings suggest that pneumococci utilize specific proteins to bind to neurons, ultimately causing neuronal cell death through intracellular calcium levels and disruption of the actin cytoskeleton. Antibodies against beta-actin may serve as a potential therapy to prevent neuronal death caused by pneumococcal infection.
Article
Medicine, Research & Experimental
Ting Guo, Chun-sun Jiang, Shan-Zhong Yang, Yi Zhu, Chao He, A. Brent Carter, Veena B. Antony, Hong Peng, Yong Zhou
Summary: This study revealed a potential new mechanism by which mitochondria contribute to the progression of fibrotic lung diseases through matrix stiffness-regulated mitochondrial fission and repositioning in migrating lung fibroblasts.
Review
Biochemistry & Molecular Biology
Remo Poto, Gjada Criscuolo, Gianni Marone, Chris E. Brightling, Gilda Varricchi
Summary: Mast cells in the lung of asthmatic patients play a significant role in airway inflammation and remodeling. They activate various immune cells and modulate the activity of structural cells in the lung. Therapies targeting mast cell mediators and receptors have shown effectiveness in treating different types of asthma. Depleting mast cells has also shown promising results. These findings highlight the importance of mast cell-targeted treatments in asthma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lu Zong, Fan Yang, Siyu Liu, Yufeng Gao, Fang Xia, Meijuan Zheng, Yuanhong Xu
Summary: SFTS is a threatening infectious disease caused by a novel Bunyavirus. SFTS patients exhibit a cytokine storm and impaired immune response, but their cellular immune response remains largely unknown.
Article
Multidisciplinary Sciences
Thiago Rojas Converso, Cibelly Goulart, Dunia Rodriguez, Maria Eduarda Souza Guerra, Michelle Darrieux, Luciana C. C. Leite
Summary: The hybrid protein rPotD-PdT can induce stronger immune responses, but is not sufficient to provide full protection against highly virulent pneumococcal strains. Therefore, combination with other antigens may be necessary for sufficient protection.
Article
Cell Biology
Ara Jo, Jin Hee Bae, Yu Jeong Yoon, Tae Hun Chung, Eun-Woo Lee, Young-Ho Kim, Hea Min Joh, Jin Woong Chung
Summary: This study reveals that plasma-activated medium (PAM) promotes cell death in human lung cancer cells through the induction of ferroptosis. PAM increases intracellular and lipid ROS, leading to mitochondrial dysfunction, which can be protected by ROS scavengers and ferroptosis inhibitors.
CELL DEATH & DISEASE
(2022)
Article
Immunology
Elin Ronnberg, Avinash Ravindran, Luca Mazzurana, Yitao Gong, Jesper Safholm, Julie Lorent, Olga Dethlefsen, Ann-Charlotte Orre, Mamdoh Al-Ameri, Mikael Adner, Sven-Erik Dahlen, Joakim S. Dahlin, Jenny Mjosberg, Gunnar Nilsson
Summary: Mast cells are important in maintaining normal bodily functions and are involved in lung inflammatory diseases such as asthma. Human lung mast cells are divided into two subsets, MCT and MCTC, based on their protein expression. However, very little is known about the heterogeneity of these cells. In this study, single cell RNA sequencing was performed to analyze the gene expression profiles of sorted human lung mast cells. The results showed high expression of classical mast cell markers, and significant variation in the expression of individual genes. Although no distinct subpopulations were identified, certain protease-encoding genes showed different levels of expression. The findings suggest that human lung mast cells are primarily of the MCT subset, and the expression of certain genes is not always consistent with protein expression.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Yun Cao, Clayton H. Rische, Bruce S. Bochner, Jeremy A. O'Sullivan
Summary: Siglec-8 is a receptor that binds to sialic acid and has different inhibitory activities on eosinophils and mast cells. Binding of Siglec-8 to antibodies induces cell death in primed eosinophils, but inhibits cellular activation in mast cells. Removal of specific sialylated ligands on cell surfaces alters the effects of Siglec-8 and induces mast cell death without cytokine priming.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Janine M. DeBlasi, Aimee Falzone, Samantha Caldwell, Nicolas Prieto-Farigua, Justin R. Prigge, Edward E. Schmidt, Iok In Christine Chio, Florian A. Karreth, Gina M. DeNicola
Summary: Mutations in the KEAP1-NRF2 pathway are common in NSCLC and contribute to therapy resistance and poor outcomes. The impact of KEAP1 and NRF2 mutations on lung tumor initiation and progression was comprehensively investigated using mouse models. The study revealed the context-dependence and activity threshold for NRF2 in the lung tumorigenic process.
Article
Cell Biology
Shigetoshi Yokoyama, Shun Nakayama, Lei Xu, Aprile L. Pilon, Shioko Kimura
Summary: SCGB3A2 activates the non-canonical inflammasome pathway to eliminate human cancer cells, indicating its potential as a novel therapeutic approach in cancer treatment.
CELL DEATH DISCOVERY
(2021)
Article
Biology
Somruethai Sumkhemthong, Eakachai Prompetchara, Pithi Chanvorachote, Chatchai Chaotham
Summary: This study reveals the potential role of hydroxyl radicals as a key mediator in the autophagic response to cisplatin treatment in lung cancer. The use of autophagy inhibitors or specific hydroxyl radical scavengers can effectively intervene in cisplatin-induced cell apoptosis and autophagosome formation, pointing towards the development of novel therapies for lung cancer.
BIOLOGICAL RESEARCH
(2021)
Article
Biology
Erin Alvi, Ayako L. Mochizuki, Yoko Katsuki, Minori Ogawa, Fei Qi, Yusuke Okamoto, Minoru Takata, Anfeng Mu
Summary: This study finds that mouse Slfn8 and Slfn9 genes may have a similar function to human SLFN11, being recruited to damaged DNA tracks and accelerating DNA repair process. This finding contributes to a better understanding of the biological role of SLFN11 through mouse modeling.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yoshiaki Sato, Hironori Yoshino, Ikuo Kashiwakura, Eichi Tsuruga
Summary: The RLR agonist Poly(I:C) was found to modulate the cellular radiation response of lung adenocarcinoma cells by downregulating DAP3 expression. Experimental results indicated that DAP3 is involved in the resistance of lung adenocarcinoma cells to IR-induced cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Daniel R. Calabrese, Emily Aminian, Benat Mallavia, Fengchun Liu, Simon J. Cleary, Oscar A. Aguilar, Ping Wang, Jonathan P. Singer, Steven R. Hays, Jeffrey A. Golden, Jasleen Kukreja, Daniel Dugger, Mary Nakamura, Lewis L. Lanier, Mark R. Looney, John R. Greenland
Summary: NK cells play a crucial role in pulmonary ischemia-reperfusion injury by binding to the NKG2D receptor. Therapies targeting NK cells may hold promise in acute lung injury.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Chemistry, Multidisciplinary
Jianran Hu, Ping Li, Baozhong Shi, Jun Tie
Summary: Saikosaponin D (SSD) can enhance cisplatin sensitivity of gastric cancer cells by inducing apoptosis and autophagy, and inhibiting the IKK beta/NF-κB signaling pathway.
Article
Critical Care Medicine
Liam G. Heaney, John Busby, Peter Bradding, Rekha Chaudhuri, Adel H. Mansur, Robert Niven, Ian D. Pavord, John T. Lindsay, Richard W. Costello
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2019)
Article
Engineering, Biomedical
Eunice Chee, Seema Nandi, Kimberly Nellenbach, Emily Mihalko, Douglas B. Snider, Landon Morrill, Andrew Bond, Erin Sproul, Jennifer Sollinger, Glenn Cruse, Maureane Hoffman, Ashley C. Brown
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2020)
Article
Cell Biology
Santosh K. Mishra, Joshua J. Wheeler, Saumitra Pitake, Huiping Ding, Changyu Jiang, Tomoki Fukuyama, Judy S. Paps, Patrick Ralph, Jacob Coyne, Michelle Parkington, Jennifer DeBrecht, Lauren C. Ehrhardt-Humbert, Glenn P. Cruse, Wolfgang Baeumer, Ru-Rong Ji, Mei-Chuan Ko, Thierry Olivry
Article
Cell Biology
Greer K. Arthur, Lauren C. Ehrhardt-Humbert, Douglas B. Snider, Corey Jania, Stephen L. Tilley, Dean D. Metcalfe, Glenn Cruse
CELLULAR SIGNALLING
(2020)
Article
Cell & Tissue Engineering
Emily W. Ozpinar, Ariana L. Frey, Greer K. Arthur, Camilo Mora-Navarro, Andreea Biehl, Douglas B. Snider, Glenn Cruse, Donald O. Freytes
Summary: Mast cells are pro-inflammatory tissue-resident immune cells that play a key role in inflammation and show significant variability in response to environmental stimuli due to in situ maturation in tissue. Studying mature tissue-derived MCs in humans poses challenges, but using decellularized ECM scaffolds can provide a more physiologically relevant environment for culture.
TISSUE ENGINEERING PART A
(2021)
Article
Cell & Tissue Engineering
Emily W. Ozpinar, Ariana L. Frey, Glenn Cruse, Donald O. Freytes
Summary: Tissue engineers often utilize biomaterials to aid wound healing, with a focus on modulating inflammation. Mast cells play an important role in this process by recognizing antigens and releasing granules that affect various aspects of tissue repair. Understanding mast cell interactions with biomaterials can enhance the design of optimized implants for tissue repair and regeneration.
TISSUE ENGINEERING PART B-REVIEWS
(2021)
Article
Biotechnology & Applied Microbiology
Douglas B. Snider, Greer K. Arthur, Guido H. Falduto, Ana Olivera, Lauren C. Ehrhardt-Humbert, Emmaline Smith, Cierra Smith, Dean D. Metcalfe, Glenn Cruse
Summary: This study developed an innovative approach using chemically stable exon skipping oligonucleotides (ESOs) to target KIT expression, which resulted in downregulation of KIT, inhibition of MC proliferation, and induction of apoptosis in neoplastic cells. In vivo administration of KIT targeting ESOs significantly inhibited tumor growth and organ infiltration, suggesting its potential as a therapeutic strategy for KIT-associated malignancies.
Article
Medicine, Research & Experimental
Xiaomei Kong, William C. Bennett, Corey M. Jania, Kelly D. Chason, Zachary German, Jennifer Adouli, Samuel D. Budney, Brandon T. Oby, Catharina van Heusden, Eduardo R. Lazarowski, Ilona Jaspers, Scott H. Randell, Barry A. Hedgespeth, Glenn Cruse, Xiaoyang Hua, Stephen A. Schworer, Gregory J. Smith, Samir Np Kelada, Stephen L. Tilley
Summary: This study shows that ozone induces mast cell degranulation and bronchial hyperresponsiveness, with P2X7 receptors playing a crucial role in the process.
Article
Cell Biology
Joana Nogueira-Rodrigues, Sergio C. Leite, Rita Pinto-Costa, Sara C. Sousa, Liliana L. Luz, Maria A. Sintra, Raquel Oliveira, Ana C. Monteiro, Goncalo G. Pinheiro, Marta Vitorino, Joana A. Silva, Sonia Simao, Vitor E. Fernandes, Jan Provaznik, Vladimir Benes, Celia D. Cruz, Boris Safronov, Ana Magalhaes, Celso A. Reis, Jorge Vieira, Cristina P. Vieira, Gustavo Tiscornia, Ines M. Araujo, Monica M. Sousa
Summary: The spiny mouse is capable of spontaneous and fast restoration of function after severe spinal cord injury (SCI) due to the formation of scarless regenerative tissue. Transcriptomic analysis revealed the rewiring of glycosylation biosynthetic pathways in the spiny mouse, leading to pro-regenerative proteoglycan signature at the SCI site. This study highlights the importance of glycosylation switch in axon regeneration after SCI.
DEVELOPMENTAL CELL
(2022)
Review
Biochemistry & Molecular Biology
Greer K. Arthur, Glenn Cruse
Summary: Mast cells are immune cells involved in allergic inflammatory response. This review focuses on IgE-mediated activation and discusses the structure and function of Fc epsilon RI, as well as the role of Fc epsilon RI beta in regulating mast cell function and signaling. Current and emerging approaches to target IgE and Fc epsilon RI signaling are discussed, along with the potential therapeutic use of alternative splicing of Fc epsilon RI beta.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Fayez Alghofaili, Hastyar Najmuldeen, Banaz O. Kareem, Bushra Shlla, Vitor E. Fernandes, Morten Danielsen, Julian M. Ketley, Primrose Freestone, Hasan Yesilkaya
Summary: This study uncovered the host signal triggering the transition of Streptococcus pneumoniae from a commensal to a parasitic state as catecholamine stress hormones. Stress hormone treatment of this microbe results in reduced cell size and capsule synthesis, enhancing its ability to migrate from the nasopharynx into the lungs. The microbe requires the TCS09 protein for recognition and processing of stress hormone signals.
Article
Neurosciences
Marta Vitorino, Sonia Simao, Joao B. Moreira, Joana Nogueira-Rodrigues, Joana Silva, Ana Sofia Lourenco, Vitor Fernandes, Monica M. Sousa, Gustavo Tiscornia, Ines M. Araujo
Summary: This study presents a coronal brain atlas of the African spiny mouse, providing a new tool for neuroanatomical research and opening new avenues for investigating its regenerative ability in brain disorders.
JOURNAL OF COMPARATIVE NEUROLOGY
(2022)
Article
Allergy
Katie Bitting, Barry Hedgespeth, Lauren C. Ehrhardt-Humbert, Greer K. Arthur, Alicia G. Schubert, Peter Bradding, Stephen L. Tilley, Glenn Cruse
Summary: This study found that the Fc epsilon RI beta-like protein MS4A6A exists in human mast cells and can compensate for the role of Fc epsilon RI beta in trafficking and signaling.
Article
Immunology
Barry A. A. Hedgespeth, Douglas B. Snider, Katie J. Bitting, Glenn Cruse
Summary: This article introduces a oligonucleotide called KitStop, which can safely reduce the severity and duration of anaphylactic response by reducing the number of mast cells in tissues. The study shows that KitStop significantly reduces mast cell numbers in the skin and peritoneum, and mice treated with KitStop experience a significantly diminished anaphylactic response in a model of passive systemic anaphylaxis compared to control mice.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Allergy
Lizette M. Cortes, David Brodsky, Celine Chen, Tiffany Pridgen, Jack Odle, Douglas B. Snider, Glenn Cruse, Arina Putikova, Mia Y. Masuda, Alfred D. Doyle, Benjamin L. Wright, Harry D. Dawson, Anthony Blikslager, Evan S. Dellon, Scott M. Laster, Tobias Kaser
Summary: This study aimed to establish swine as a large animal model for EoE and demonstrated that swine can develop immunological and pathological markers similar to human EoE after sensitization and challenge treatment.
FRONTIERS IN ALLERGY
(2022)