期刊
JOURNAL OF IMMUNOLOGY
卷 186, 期 1, 页码 222-229出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1002597
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资金
- National Institutes of Health [AI044095]
- Agency for Science, Technology, and Research (Singapore)
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R56AI084939, R56AI044095, R01AI044095] Funding Source: NIH RePORTER
NK cells are innate immune cells that are important in tumor immunity, but also have the ability to modulate the adaptive immune system through cytokine production or direct cell-cell interactions. This study investigates the interaction of NK cells with dendritic cells (DCs) and tumor cells, and the role of specific NK cell-activating receptors in this process. Primary rat NK cells and an NK cell line produced IFN-gamma when cocultured with either DCs or the rat hepatoma cell line McA-RH7777 (McA). This NK cell activation by DCs and McA required cell-cell contact and was dependent on distinct NK-activating receptors. Silencing NK cell expression of NKp46 and NKp30 significantly diminished DC-and McA-mediated NK cell IFN-gamma production, respectively. NK cells killed immature and mature DCs independently of NKp46, NKp30, and NKG2D; however, cytotoxicity against McA cells was dependent on NKp30 and NKG2D. Thus, we have shown in this study that NKp30 plays dual activating roles in NK-McA tumor interactions by mediating cytokine production and cytotoxicity. More importantly, NK cells are activated by both DCs and hepatoma cells to produce IFN-gamma, but require distinct NK cell-activating receptors, NKp46 and NKp30, respectively. Our data suggest that therapeutics could be developed specifically to target NK-DC interactions without compromising NK tumor immunity. The Journal of Immunology, 2011, 186: 222-229.
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