Article
Biochemistry & Molecular Biology
Xue-Wei Zhang, Ming-Xin An, Zeng-Kang Huang, Lan Ma, Dan Zhao, Zhao Yang, Jun-Xiu Shi, Dong-Xin Liu, Qiang Li, An-Hua Wu, Yu-Hua Chen, Wei-Dong Zhao
Summary: This study shows that deletion of the Lpp gene in E. coli reduces its pathogenicity in causing neonatal meningitis. The Lpp-deleted E. coli is susceptible to neutrophil bactericidal activity, with increased production of ROS and activation of NADPH oxidase. This enhanced survival is associated with the upregulation of FliC and suppression of NADPH oxidase activation in neutrophils.
Article
Multidisciplinary Sciences
Mutasim E. Ibrahim
Summary: This study investigated the prevalence and risk factors for multidrug-resistant Klebsiella pneumoniae infection among hospitalized patients in southern Saudi Arabia. The study found that 66.8% of patients were infected with multidrug-resistant K. pneumoniae strains. Factors such as gender, age, intensive care unit admission, invasive medical devices, and chronic illness were significantly associated with the infection.
SCIENTIFIC REPORTS
(2023)
Review
Immunology
Marc Bonten, James R. Johnson, Anita H. J. van den Biggelaar, Leonidas Georgalis, Jeroen Geurtsen, Patricia Ibarra de Palacios, Stefan Gravenstein, Thomas Verstraeten, Peter Hermans, Jan T. Poolman
Summary: The study revealed a significant burden of E. coli bacteremia in older adults in high-income countries, especially in individuals aged 55 and above. Implementing community-level prevention programs can effectively prevent E. coli bacteremia and urinary tract infections in this age group.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Joshua T. Thaden, Richard Ahn, Felicia Ruffin, David W. Gjertson, Alexander Hoffmann, Vance G. Fowler, Michael R. Yeaman
Summary: This study identifies reduced host transcriptional signatures involving mitochondrial energy transduction in methicillin-resistant Staphylococcus aureus compared to methicillin-susceptible Staphylococcus aureus. Patients with gram-negative infection had increased activation of the classic complement system.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Agriculture, Multidisciplinary
Hong Pan, He Li, Shaoting Wu, Chongde Lai, Daoyi Guo
Summary: In this study, a biosynthetic pathway for the production of anisyl alcohol from simple carbon sources was established in engineered Escherichia coli MG1655. Anisyl alcohol production was further increased to 355 mg/L by increasing the availability of erythrose-4-phosphate and phosphoenolpyruvate. Additionally, anisyl acetate was produced by overexpressing alcohol acetyltransferase ATF1 for the acetylation of anisyl alcohol. This is the first report on the biosynthesis of anisyl alcohol and anisyl acetate directly from a renewable carbon source.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Infectious Diseases
Qin Zou, Hua Zou, Yan Shen, Lang Yu, Wei Zhou, Chenglin Sheng, Ang Liao, Chunli Li
Summary: This study retrospectively analyzed pathogen distribution and drug sensitivity among postpartum patients with bloodstream infections in Chongqing, China from January 2015 to December 2020. Escherichia coli was found to be the most common gram-negative bacillus in postpartum patients, and resistance increased against commonly used drugs while remaining unchanged for less commonly used drugs.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Andre Becker S. Saidenberg, Arnoud H. M. van Vliet, Marc Stegger, Thor Bech Johannesen, Torsten Semmler, Marcos Cunha, Alessandro C. de O. Silveira, Eleine Kuroki Anzai, Isabel C. A. Scaletsky, Anders Dalsgaard, Roberto M. La Ragione, Terezinha Knobl
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen associated with human and companion animal diseases, while avian pathogenic pathotype (APEC) severely impacts poultry health and production. The similarities between APEC and human ExPEC suggest a potential for zoonotic transmission.
VETERINARY MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Rebecca N. Evans, Jessica Harris, Chris A. Rogers, Alasdair MacGowan
Summary: The aim of this study was to emulate the MERINO trial comparing the efficacy of piperacillin-tazobactam and meropenem for the treatment of bloodstream infection caused by ceftriaxone-nonsusceptible E coli or Klebsiella spp. The results showed that the mortality rate in the emulated trial population was higher than that in the MERINO trial, and the effect size was smaller after adjustment for propensity score.
Article
Biochemistry & Molecular Biology
Meng-Xi Wang, Li Yan, Juan Chen, Jun-Mei Zhao, Jiang Zhu, Shan-He Yu
Summary: This study explored the feasibility of reducing the malignant characteristics of t(8;21) AML cells by reinforcing their endogenous differentiation potential. The results showed that in an AE9a murine model, the process of erythroid differentiation was accompanied by a decline or loss of leukemia-initiating potential. Therefore, enhancing the erythroid differentiation of t(8;21) AML cells may be a promising intervention strategy.
Article
Biotechnology & Applied Microbiology
Baodong Hu, Xinrui Zhao, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du
Summary: This study reports the development of a bacterial P450 sca-2(mut) whole-cell biocatalyst with remarkable 3'-hydroxylation activity for efficient hydroxylation of flavonoids. Flavodoxin Fld and flavodoxin reductase Fpr from Escherichia coli were used to enhance the whole-cell activity of sca-2(mut). Through enzymatic engineering, the double mutant of sca-2(mut) (R88A/S96A) exhibited improved hydroxylation performance for flavonoids. The optimized whole-cell biocatalytic conditions further enhanced the whole-cell activity of sca-2(mut) (R88A/S96A). Eriodictyol, dihydroquercetin, luteolin, and 7,3',4'-trihydroxyisoflavone were successfully produced as functionalized derivatives of flavonoids using naringenin, dihydrokaempferol, apigenin, and daidzein as substrates, with conversion yields of 77%, 66%, 32%, and 75% respectively. The strategy employed in this study offers an effective method for the hydroxylation of other high value-added compounds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Xinghong Zhao, Xinyi Zhong, Shinong Yang, Kai Deng, Lu Liu, Xu Song, Yuanfeng Zou, Lixia Li, Xun Zhou, Renyong Jia, Juchun Lin, Huaqiao Tang, Gang Ye, Jianqing Yang, Shan Zhao, Yifei Lang, Hongping Wan, Zhongqiong Yin, Oscar P. Kuipers
Summary: Brevicidine, a bacterial cyclic lipopeptide, exhibits potent and selective antimicrobial activity against Gram-negative pathogens. However, little was known about its mechanism of action before our investigations. We found that brevicidine exerts its bactericidal activity by interacting with lipopolysaccharide, targeting phosphatidylglycerol and cardiolipin, and disrupting the proton motive force of bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Amanda Brumwell, Granger Sutton, Paul M. M. Lantos, Kate Hoffman, Felicia Ruffin, Lauren Brinkac, Thomas H. H. Clarke, Mark D. D. Adams, Vance G. G. Fowler Jr, Derrick E. E. Fouts, Joshua T. T. Thaden
Summary: This study aims to further understand the risk factors, clinical outcomes, and bacterial genetics associated with ST131 bloodstream infections. A cohort study was conducted in which 88 out of 227 patients with E. coli bloodstream infection were infected with ST131. There was no significant difference in in-hospital mortality between patients with ST131 and non-ST131 infections overall, but ST131 was associated with higher mortality in patients with urinary tract infections.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Biology
Julia Schanin, Wouter Korver, Emily C. Brock, John Leung, Zachary Benet, Thuy Luu, Katherine Chang, Alan Xu, Naomi De Freitas, Kenneth Luehrsen, Michael A. Brehm, Alan Wong, Bradford A. Youngblood
Summary: This study identified an agonistic Siglec-6 mAb that induces inhibition and reduction of mast cells through Fc-mediated interaction. The findings suggest that targeting Siglec-6 with agonistic antibodies may be a potential therapeutic approach for allergic diseases.
COMMUNICATIONS BIOLOGY
(2022)
Article
Medicine, General & Internal
Nick Daneman, Daniel Fridman, Jennie Johnstone, Bradley J. Langford, Samantha M. Lee, Derek M. MacFadden, Kwadwo Mponponsuo, Samir N. Patel, Kevin L. Schwartz, Kevin A. Brown
Summary: This study investigated patients with E.coli bloodstream infections in Ontario, Canada from 2017 to 2020, and found that resistance to multiple antibiotics is associated with a significant increase in mortality risk among patients.
Article
Microbiology
Rory Cave, Mary M. Ter-Stepanyan, Nune Kotsinyan, Hermine Mkrtchyan
Summary: We conducted whole-genome sequencing and analysis of ESBL UPEC ST127 isolates recovered from patients in hospitals in Armenia. Our findings indicate that E. coli ST127 was a dominant lineage causing urinary tract infections in different regions of Armenia. We also identified unique genes in Armenian and French isolates that were absent in other ST127 isolates obtained from the European Nucleotide Archive (ENA). These findings suggest a possible cross-country transmission and highlight the importance of implementing whole-genome sequencing surveillance in tackling antibiotic resistance.
MICROBIOLOGY SPECTRUM
(2022)
Editorial Material
Medical Laboratory Technology
Tiffany Yin, Caroline Raasch Alquist
CLINICAL CHEMISTRY
(2020)
Article
Hematology
Victoria Simenson, Jeffrey Burton, Alejandra Del Toro, Caroline Raasch Alquist
JOURNAL OF CLINICAL APHERESIS
(2020)
Editorial Material
Immunology
Bing Han, Caroline R. Alquist
CLINICAL INFECTIOUS DISEASES
(2021)
Letter
Hematology
Kristina Prus, Caroline R. Alquist, Jose A. Cancelas, David Oh
Article
Medical Laboratory Technology
Caroline R. Alquist, Louise Helander
CLINICS IN LABORATORY MEDICINE
(2021)
Article
Hematology
Kristina Prus, Krystol Weidner, Caroline Alquist
Summary: This study investigated the response of 16 late adolescent and adult PANDAS/PANS patients to TPE and found that most patients showed improvement after treatment.
JOURNAL OF CLINICAL APHERESIS
(2022)
Article
Hematology
Ryan D. Salazar, Krystol R. Weidner, Caroline R. Alquist
Summary: Susac's syndrome is an autoimmune endotheliopathy characterized by encephalopathy, hearing loss, and branch retinal artery occlusion. Its pathogenesis and treatment methods are still uncertain, but therapeutic plasma exchange may be an effective adjunctive treatment.
JOURNAL OF CLINICAL APHERESIS
(2023)
Meeting Abstract
Surgery
H. Shaikh, J. Awosika, B. Barakoti, B. Hambley, C. Alquist
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Meeting Abstract
Surgery
P. Shelat, C. Alquist
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Meeting Abstract
Surgery
M. McGowan, A. Bickenbach, A. R. Shields, R. R. Alloway, P. Brailey, E. Portwood, C. Alquist, A. Christianson, B. Abu Jawdeh, M. Cuffy, J. Kremer, S. Bumb, A. Govil, M. Anand, T. Kaur, E. S. Woodle
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Meeting Abstract
Hematology
Laura Cooling, Nicole L. Draper, Caroline Alquist, Andrew Jones, Leonor Fernando, Yara Park, Nancy M. Dunbar, Joseph Schwartz, Elizabeth A. Godbey, Haewon C. Kim, Amy E. Schmidt, Esther Lee, Charles Harmon, Briana Gibson, Yevgeniy Linnik, Laura S. Connelly-Smith
Review
Medicine, General & Internal
Gordon Wadge, Jenny Zhang, John Seal, Edgar Shannon Cooper, Caroline R. Alquist
Summary: Directed blood donation, despite its drawbacks such as transfusion-transmitted infection risk and administrative burdens, still plays a role in specific patient populations like those with rare blood types or immunoglobulin A deficiencies due to the challenges in finding compatible blood for transfusion. Clinicians should carefully weigh the risks and benefits when discussing directed blood donations with patients and family members.
Meeting Abstract
Surgery
Kelley Nunez, Sarah Robertson, Jamie Cherry, E. Shannon Cooper, Paul Thevenot, Ari Cohen, Caroline Alquist
AMERICAN JOURNAL OF TRANSPLANTATION
(2020)
Meeting Abstract
Hematology
Victoria Simenson, Alejandra Del Toro, Caroline Raasch Alquist
Meeting Abstract
Gastroenterology & Hepatology
Kelley Nunez, Sarah Robertson, Jamie Cherry, Shannon Cooper, Paul Thevenot, Ari J. Cohen, Caroline Alquist