Article
Cell Biology
Yang Xie, Yuan Zhang, Tianxiao Zhu, Jingchang Ma, Chujun Duan, Lu Yang, Tingting Wang, Ran Zhuang, Ka Bian, Lianjun Lu
Summary: This study revealed the crucial role of CD226 in allergic inflammatory responses in a murine AR model. Specifically, CD226 may regulate ILC2 function to alleviate allergic and inflammatory responses, rather than affecting CD4(+) T cells.
MEDIATORS OF INFLAMMATION
(2022)
Article
Chemistry, Analytical
Emma B. Hartnett, Mengli Zhou, Yi-Nan Gong, Yu-Chih Chen
Summary: The study developed a label-free method to classify cell death status using machine learning and image processing. It enables the precise categorization of live, apoptotic, and necrotic cells and allows for tracking the time dynamics of cell death process, enhancing the understanding of cell death regulation at the molecular level.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Medicinal
Feihong Ji, Xinguang Qiu
Summary: This paper summarizes recent research on the mechanisms of thyroid diseases and the role of NAPCD in treatment, helping to better understand the pathophysiology of thyroid diseases and providing guidance for the development of new therapeutic strategies in the future.
Article
Biology
Talang Wang, Ruoyu Zhao, Junhong Zhi, Ziling Liu, Aiwei Wu, Zimei Yang, Weixu Wang, Ting Ni, Lili Jing, Ming Yu
Summary: Tox4 protein is a regulator of PP1 phosphatases with unknown function in development. In mice, conditional knockout of Tox4 reduces thymic cellularity, partially blocks T cell development, and decreases the ratio of CD8 to CD4 cells. Tox4 loss also impairs proliferation of the fast-proliferating DP blast population within DP cells through downregulation of genes critical for proliferation, and genes with high and low expression level are more dependent on Tox4. Mechanistically, Tox4 may facilitate transcriptional reinitiation and restrict elongation in a dephosphorylation-dependent manner, and it is an evolutionarily conserved regulator of transcriptional elongation and reinitiation. Tox4 controls T cell development in mice by regulating transcription of cell cycle regulation genes via PP1 phosphatases, independent of chromatin accessibility modulation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Immunology
Jingchang Ma, Yitian Liu, Chujun Duan, Shuwen Wu, Yang Xie, Lu Yang, Xuemei Li, Yuling Wang, Yuan Zhang, Ran Zhuang
Summary: The costimulation molecule CD226 plays a significant role in T cell differentiation, activation, and immune functional regulation. Its deficiency impairs immune response capacity and affects thymocyte development, particularly the maturation of CD8+ T cells.
Article
Immunology
David L. Owen, Rebecca S. La Rue, Sarah A. Munro, Michael A. Farrar
Summary: Recent studies have revealed multiple stages of regulatory T cell development in the thymus, with both CD25+ T cell progenitors and FOXP3lo T cell progenitors undergoing agonist-activated states and transitional stages before differentiating into mature T cells. CD25+ T cell progenitors were found to be the major source of IL-2 required for the conversion of progenitors into mature T cells. Additionally, thymic resident regulatory T cells were found to be heterogeneous and exhibit the most diverse T cell receptor repertoire among CD4 single-positive thymocytes.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Fabian Klein, Clara Veiga-Villauriz, Anastasiya Borsch, Stefano Maio, Sam Palmer, Fatima Dhalla, Adam E. E. Handel, Saulius Zuklys, Irene Calvo-Asensio, Lucas Musette, Mary E. E. Deadman, Andrea J. J. White, Beth Lucas, Graham Anderson, Georg A. A. Hollander
Summary: The network of thymic stromal cells plays a crucial role in T cell development and selection. By employing advanced techniques such as flow cytometry and machine learning, the researchers identified novel subpopulations of thymic epithelial cells and characterized their functions, particularly in perinatal cortical TEC.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Jordan F. Hastings, Sharissa L. Latham, Alvin Kamili, Madeleine S. Wheatley, Jeremy Z. R. Han, Marie Wong-Erasmus, Monica Phimmachanh, Max Nobis, Chiara Pantarelli, Antonia L. Cadell, Yolande E. I. O'Donnell, King Ho Leong, Sophie Lynn, Fan-Suo Geng, Lujing Cui, Sabrina Yan, Joanna Achinger-Kawecka, Clare Stirzaker, Murray D. Norris, Michelle Haber, Toby N. Trahair, Frank Speleman, Katleen De Preter, Mark J. Cowley, Ozren Bogdanovic, Paul Timpson, Thomas R. Cox, Walter Kolch, Jamie I. Fletcher, Dirk Fey, David R. Croucher
Summary: Gene expression noise promotes stochastic drug resistance in rare cancer cells. However, when integrated across multiple components of an apoptotic signaling network, the influence of noise leads to a higher frequency of chemoresistant neuroblastoma cells. These cells are characterized by JNK impairment and retain a memory of their resistant state even after chemotherapy treatment.
Article
Multidisciplinary Sciences
Christopher Szeto, Pirooz Zareie, Rushika C. Wirasinha, Justin B. Zhang, Andrea T. Nguyen, Alan Riboldi-Tunnicliffe, Nicole L. La Gruta, Stephanie Gras, Stephen R. Daley
Summary: This study describes the contribution of natural covalent bonds between TCR and antigenic peptides in T cell differentiation and activation. The formation of disulfide bonds between TCR and peptide does not require structural rearrangement and can still occur when the initial affinity is low, facilitating T cell activation.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Jeffrey W. Pippin, Natalya Kaverina, Yuliang Wang, Diana G. Eng, Yuting Zeng, Uyen Tran, Carol J. Loretz, Anthony Chang, Shreeram Akilesh, Chetan Poudel, Hannah S. Perry, Christopher O'Connor, Joshua C. Vaughan, Markus Bitzer, Oliver Wessely, Stuart J. Shankland
Summary: With an aging population, kidney health becomes an important medical and socioeconomic factor. The study found that PD-1 signaling plays a crucial role in kidney aging and in the development of focal segmental glomerulosclerosis.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Virology
Jeferson da Costa Lopes, Luiz Fabio Magno Falcao, Arnaldo Jorge Martins Filho, Marcos Luiz Gaia Carvalho, Caio Cesar Henriques Mendes, Fabio Alves Olimpio, Vanessa do Socorro Cabral Miranda, Lais Carneiro Dos Santos, Jannifer Oliveira Chiang, Ana Cecilia Ribeiro Cruz, Vanessa Costa Alves Galucio, Raimunda do Socorro da Silva Azevedo, Livia Caricio Martins, Maria Irma Seixas Duarte, Jorge Rodrigues de Sousa, Pedro Fernando da Costa Vasconcelos, Juarez Antonio Simoes Quaresma
Summary: This study identified and quantified proteases and protein targets involved in apoptosis cascades triggered by YFV infection in human hepatocytes using immunohistochemistry. The complexity of YFV infection-induced apoptosis and its potential contribution to the pathogenesis of fatal YF in humans were confirmed.
Article
Biochemistry & Molecular Biology
Jinning Li, Xiaoming Zhao, Bing Wu, Zhongpeng Ji, Han Liu, Xuehan Wang, Haibing Zhang, Zhiping He
Summary: Cell death detection is traditionally invasive, but this study demonstrates the non-invasive use of near-infrared spectroscopy to distinguish between different types of cell death. By measuring the scattering of NIR light in the wavelength range of 1100-1700 nm, the study successfully differentiated between normal, apoptotic, and necroptotic mouse dermal fibroblast cells. The attenuation coefficient (8μ) was used to quantify the ease of light passing through the cells and confirmed the distinction between cell death types.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2023)
Review
Microbiology
Xin-yu Zhang, Yu-ying Li, Hai-xin Huang, Chen-chen Zhao, Xiao-xiao Lei, Bao-peng Zhao, Jing-yi Lu, Tian Lan, Wen-chao Sun
Summary: This article reviews the functions of Seneca Valley virus 3C protease, including promotion of viral replication, modulation of cell apoptosis, and evasion of host immune response, providing a theoretical basis for preventing and controlling SVV infection.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Hao Yang, Shan Xu, Lidan Tang, Jinhong Gong, Hufeng Fang, Jifu Wei, Dan Su
Summary: This review summarizes the current status of research on the molecular mechanisms and targets of quercetin-mediated non-apoptotic cell death modes.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Jingchang Ma, Wei Hu, Yitian Liu, Chujun Duan, Dongliang Zhang, Yuling Wang, Kun Cheng, Lu Yang, Shuwen Wu, Boquan Jin, Yuan Zhang, Ran Zhuang
Summary: This study demonstrates that CD226 plays a role in maintaining the stability and metabolism of Treg cells under inflammatory conditions. Treg cell-specific deletion of CD226 weakens immunosuppression and leads to a change in phenotype. This effect is mediated by the AMPK/mTOR/Myc pathway. Restoring Myc signaling can improve the impaired functions of CD226-deficient Treg cells in a colitis model.
Article
Biochemistry & Molecular Biology
Qing-shan Liang, Jian-Gang Xie, ChaoPing Yu, ZhuSheng Feng, JingChang Ma, Yuan Zhang, Dong Wang, JianGuo Lu, Ran Zhuang, Jikai Yin
Summary: Splenectomy reduces liver fibrosis by decreasing the expression of LIGHT, a factor that fuels fibrosis, thereby improving liver disease.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Shuwen Wu, Yuan Zhang, Jingchang Ma, Yongming Liu, Wenpeng Li, Tingting Wang, Xuexue Xu, Yuling Wang, Kun Cheng, Ran Zhuang
Summary: This study reveals the important role of IL-6 in mucosal immune cells and its interaction with gut microbiota. IL-6 loss can alter the composition of gut microbiota and affect the maintenance of immune cells, triggering mucosal immune response.
Article
Multidisciplinary Sciences
Yang Xie, Yuan Zhang, Yitian Liu, Yuling Wang, Kun Cheng, Ran Zhuang, Ka Bian
Summary: ILC2s have been extensively studied and found to be involved in regulating various pathological processes. However, their exact functions in nasal mucosal immunity and their potential role in related diseases remain unknown. This study established a method to isolate and identify ILC2s from the nasal mucosa and detected CD226 expression on these cells. This protocol will help explore the internal pathological mechanism of immunological disorders in nasal mucosal diseases.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Yang Xie, Yuan Zhang, Tianxiao Zhu, Jingchang Ma, Chujun Duan, Lu Yang, Tingting Wang, Ran Zhuang, Ka Bian, Lianjun Lu
Summary: This study revealed the crucial role of CD226 in allergic inflammatory responses in a murine AR model. Specifically, CD226 may regulate ILC2 function to alleviate allergic and inflammatory responses, rather than affecting CD4(+) T cells.
MEDIATORS OF INFLAMMATION
(2022)
Article
Immunology
Pengcheng Li, Chunmei Zhang, Meng Wang, Xiyue Zhang, Yusi Zhang, Kang Tang, Haifeng Hu, Xiaozhou Jia, Ran Zhuang, Boquan Jin, Ying Ma, Yun Zhang
Summary: This study analyzed the role of neutrophils in HFRS patients. The results showed that plasma MPO and NPA significantly increased in the acute phase and critical/severe groups of HFRS patients and were correlated with inflammatory and renal injury-related clinical parameters. These findings provide new insights into the potential role of neutrophils in the pathogenesis of HFRS.
Article
Biochemistry & Molecular Biology
Yuan Zhang, Xuexue Xu, Jingchang Ma, Yongming Liu, Chujun Duan, Yitian Liu, Chongyang Feng, Wenpeng Li, Yuling Wang, Kun Cheng, Ran Zhuang
Summary: CD226 plays a crucial role in the development of atherosclerosis by promoting foam cell formation. CD226 deficiency reduces plaque accumulation and inhibits atherosclerosis progression.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Virology
Huiyuan Zhang, Yazhen Wang, Ying Ma, Kang Tang, Chunmei Zhang, Meng Wang, Xiyue Zhang, Manling Xue, Xiaozhou Jia, Haifeng Hu, Na Li, Ran Zhuang, Boquan Jin, Lihua Chen, Yun Zhang, Yusi Zhang
Summary: This study characterized double positive T (DP T) cells in HFRS patients using flow cytometry data combined with scRNA-seq data. Results showed an upregulation of DP T cells in the peripheral blood during HTNV infection, which was correlated with disease stage. The scRNA-seq data clustered and unraveled the gene expression profile of DP T cells.
Article
Immunology
Yusi Zhang, Meng Wang, Xiyue Zhang, Kang Tang, Chunmei Zhang, Xiaozhou Jia, Haifeng Hu, He Liu, Na Li, Ran Zhuang, Boquan Jin, Ying Ma, Yun Zhang
Summary: This study characterized CD8(+)MAIT cells from HFRS patients using scRNA-seq and flow cytometry. The activation and deficiency of CD8(+)MAIT cells in peripheral blood during HTNV infection were observed, which correlated with disease severity. CD8(+)MAIT cells showed anti-viral properties by producing granzyme B and IFN-gamma, and limiting HTNV replication. The activation of MAIT cells was mediated by IL-18, sourced from HTNV-infected monocytes.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2023)
Article
Immunology
Jingchang Ma, Yitian Liu, Chujun Duan, Shuwen Wu, Yang Xie, Lu Yang, Xuemei Li, Yuling Wang, Yuan Zhang, Ran Zhuang
Summary: The costimulation molecule CD226 plays a significant role in T cell differentiation, activation, and immune functional regulation. Its deficiency impairs immune response capacity and affects thymocyte development, particularly the maturation of CD8+ T cells.
Article
Infectious Diseases
Kang Tang, Yusi Zhang, Chunmei Zhang, Haifeng Hu, Ran Zhuang, Boquan Jin, Yun Zhang, Ying Ma
Summary: This study found that plasma OPG levels were elevated in patients with HFRS and correlated positively with disease severity and negatively with platelet counts. Endothelial cells released abundant OPG in response to TNF-α stimuli and HTNV infection, which correlated positively with plasma TNF-α and c-reactive protein. Increased plasma OPG levels induced by HTNV may be an important factor for the severity of HFRS and could contribute to endothelial dysfunction and hemorrhagic disorder.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Xiyue Zhang, Yusi Zhang, He Liu, Kang Tang, Chunmei Zhang, Meng Wang, Manling Xue, Xiaozhou Jia, Haifeng Hu, Na Li, Ran Zhuang, Boquan Jin, Fanglin Zhang, Yun Zhang, Ying Ma
Summary: This study aimed to investigate the effect of bystander-activated CD8+ T cell responses in HTNV infection. An in vitro infection model was established to mimic endothelium injury in HFRS patients. The results showed that HTNV infection could activate EBV or CMV-specific CD8+ T cells and induce high levels of IL-15 production in HTNV-infected HUVECs, which were correlated with disease severity and NKG2D expression on bystander-activated CD8+ T cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Yuan Zhang, Chujun Duan, Shuwen Wu, Jingchang Ma, Yongming Liu, Wenpeng Li, Tingting Wang, Lu Yang, Kun Cheng, Ran Zhuang
Summary: IL-6 deficiency alleviates acute CWIR-induced intestinal damage and apoptosis, and improves stress defense. This finding sheds new light on treating CWIR-induced intestinal disorders by inhibiting IL-6 signaling.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Surgery
Dongliang Zhang, Yitian Liu, Jingchang Ma, Zhigang Xu, Chujun Duan, Yuling Wang, Xuemei Li, Juntao Han, Ran Zhuang
Summary: This study established a transplantation rejection model to evaluate the regulation of the poliovirus receptor signaling pathway using CD226 knockout or TIGIT-Fc recombinant protein treatment. The results showed that TIGIT-Fc treatment and CD226 knockout prolonged graft survival time, increased regulatory T cell proportion, and induced M2-type macrophage polarization. Donor-reactive recipient T cells became hyporesponsive but still responded normally to a third-party antigen challenge.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Immunology
Tingting Wang, Wei Qiao, Yang Xie, Jingchang Ma, Wei Hu, Lu Yang, Xuemei Li, Chujun Duan, Shuwen Wu, Yuling Wang, Kun Cheng, Yuan Zhang, Ran Zhuang
Summary: In this study, CD226 deficiency was found to result in more severe skin pathologies and bowel abnormalities in an AD-like mouse model. CD226 expression was elevated in intestinal mucosal tissues following DNCB administration. CD226 deficiency led to an overactive intestinal immune response, characterized by increased inflammation and cytokine levels, Paneth cell numbers, and AMP expression.
Article
Microbiology
Shanshou Liu, Dongqing Wen, Chongyang Feng, Chaoping Yu, Zhao Gu, Liping Wang, Zhixiang Zhang, Wenpeng Li, Shuwen Wu, Yitian Liu, Chujun Duan, Ran Zhuang, Lihao Xue
Summary: Heat acclimation training (HAT) can prevent heat-related diseases. This study found that HAT has beneficial effects on organ function, immunity, and gut microbes, with a stronger promotion of probiotics in a high temperature and humidity environment.
FRONTIERS IN MICROBIOLOGY
(2023)