Article
Biochemistry & Molecular Biology
Huanhuan Wang, Linghao Zhang, Huaqing Sun, Shufeng Xu, Kun Li, Xin Su
Summary: In this study, we developed an inhibitory aptamer for APE1 using SELEX technology. The aptamer showed high binding affinity and could entirely inhibit the activity of APE1. These aptamers can be utilized for early cancer diagnosis and treatment, as well as a valuable tool for studying the function of APE1.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Analytical
Yuqiang Hu, Zhen Zhang, Weicong Ye, Wei Zhang, Minghao Hu, Wenqian Yuan, Hongbo Wang, Xianjin Xiao, Tongbo Wu
Summary: Human apurinic/apyrimidinic endonuclease 1 (APE1) is a key player in DNA repair and gene regulation, with abnormal variations of its concentration in human blood and tissue cells being highly correlated to various diseases. This study introduces a DNA structure-mediated fluorescent biosensor for detecting APE1 activity with high sensitivity and selectivity. Through clever design, the biosensor improves APE1 reactivity and minimizes interference from other enzymes, achieving a low detection limit of 1.7 x 10(-6) U/mL and successful application in real biological samples and screening of APE1 inhibitors.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Cell Biology
Thais Teixeira Oliveira, Fabricia Lima Fontes-Dantas, Rayssa Karla de Medeiros Oliveira, Daniele Maria Lopes Pinheiro, Leonam Gomes Coutinho, Vandeclecio Lira da Silva, Sandro Jose de Souza, Lucymara Fassarella Agnez-Lima
Summary: Chemical inhibition of APE1/Ref-1 affects gene expression in an inflammatory cellular model, reducing the expression of certain cytokines and chemokines. It also impacts transcriptional factors such as NRF1 and ETS family regulators, suggesting partial overlap between APE1 redox and DNA repair functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Suravi Pramanik, Yingling Chen, Heyu Song, Irine Khutsishvili, Luis A. Marky, Sutapa Ray, Amarnath Natarajan, Pankaj K. Singh, Kishor K. Bhakat
Summary: This study identifies a novel role for the protein APE1 in regulating stable G4 formation and KRAS expression in PDAC, suggesting that G4 structures could be potential prognostic markers and therapeutic targets for PDAC.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yu-Ran Lee, Eun Young Bae, Hong Ryang Kil, Byeong-Hwa Jeon, Geena Kim
Summary: This study investigated the association between APE1/Ref-1 and Kawasaki disease (KD). The results showed that APE1/Ref-1 levels were significantly higher in KD patients, especially in refractory KD. APE1/Ref-1 could be a beneficial biomarker for the diagnosis and prognosis of KD.
Article
Medicine, Research & Experimental
Zhou Hai, Qin Jia
Summary: The multifunctional key protein APE1 is closely associated with asthma, and the mRNA levels of APE1 in peripheral blood neutrophils can be used as a marker for diagnosing and distinguishing the disease.
INVESTIGACION CLINICA
(2022)
Review
Biochemistry & Molecular Biology
Lauren Sahakian, Ainsley M. Robinson, Linda Sahakian, Rhian Stavely, Mark R. Kelley, Kulmira Nurgali
Summary: Inflammatory bowel disease (IBD) is a chronic relapsing inflammation of the gastrointestinal tract. The prevalence of IBD is increasing worldwide, calling for the development of new treatments. APE1/Ref-1 has been identified as a potential target for IBD therapy due to its role in regulating crucial pathways in inflammatory diseases. This review discusses the current status of IBD treatments, the role of APE1/Ref-1 in intestinal inflammation, and the potential of small molecule inhibitors to modulate inflammation and oxidative stress in IBD.
Article
Medicine, General & Internal
In Seol Yoo, Yu-Ran Lee, Seong Wook Kang, Jinhyun Kim, Hee-Kyoung Joo, Su-Jin Yoo, Chan Keol Park, Ha-Reum Lee, Ji Ah Park, Byeong-Hwa Jeon
Summary: The study demonstrates that the levels of APE1/Ref-1 are significantly elevated in the serum and synovial fluid of rheumatoid arthritis patients, and are positively correlated with disease activity.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Nicole M. Hoitsma, Timothy H. Click, Pratul K. Agarwal, Bret D. Freudenthal
Summary: The study provides insights into the processing of abasic ribonucleotides (rAPs) by APE1, revealing a decrease in activity compared to canonical deoxy-AP substrates. Novel contacts between rAP and the APE1 active site were identified using X-ray crystallography, showing accommodation of the rAP sugar pucker in a C3'-endo conformation. This work advances the understanding of ribonucleotide processing within genomic DNA.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Arianna Barchiesi, Veronica Bazzani, Agata Jabczynska, Lukasz S. Borowski, Silke Oeljeklaus, Bettina Warscheid, Agnieszka Chacinska, Roman J. Szczesny, Carlo Vascotto
Summary: APE1 is a versatile protein involved in DNA repair, gene expression regulation, and RNA metabolism in mitochondria. Loss of APE1 leads to accumulation of damaged mitochondrial mRNA, impairing protein translation and mitochondrial respiration efficiency. The data demonstrate that APE1 plays a crucial role in maintaining mitochondrial well-being.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Junyu Fan, Manqi Liu, Xiaomin Li, ShengLan Gao, Yahong Wang, Ao Li, Lujun Chen, Dengshuang Zhou, Hongqiao Chen, Zhiliang Xu, Zijun Wu, Keng Wu
Summary: Cardiomyocyte apoptosis caused by fat metabolism disorder plays an essential role in the pathogenesis of diabetic cardiomyopathy. Apurinic/apyrimidinic endonuclease 1 (APE1) has multiple functions, including regulating redox and DNA repair. However, the role of APE1 in the pathogenesis of DCM remains unclear.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Yecan Pan, Rui Weng, Linghao Zhang, Jing Qiu, Xinlu Wang, Guangqin Liao, Zhaohui Qin, Lingpu Zhang, Haihua Xiao, Yongzhong Qian, Xin Su
Summary: Cellular oxidative stress caused by reactive oxygen species (ROS) and its induced molecular response is crucial in the development of various diseases and biological processes. By using DNA nanostructure-based fluorescent probes, the localization and abundance of ROS and its related molecules can be simultaneously mapped in living cells. This study introduces intramolecular Orthogonal Reporters (iOR) based on DNA nanostructure scaffolds, which eliminate undesired fluorophore interaction by precise positioning of fluorescence reporters. iOR, functionalized with nuclear localization signal peptide, enables high sensitivity and specificity in imaging ROS and its associated DNA repair enzyme (APE1) in the nucleus and cytoplasm. The study reveals a strong positive correlation and synergistic regulation between ROS and APE1 in living cells and tumor-bearing mice through direct fluorescence imaging. This work presents a new approach for developing nanostructure-based molecular probes with broad applications in cellular biology and nanotechnology.
Article
Chemistry, Analytical
Qi Kang, Xiaoyan Yang, Yumin Du, Yinxiao Qi, Zhiqiang He, Hua Xiang
Summary: In this study, a molecular gated hyperbranched rolling circle amplification (HRCA) quick sensing system was designed for rapid detection of APE1. The system exhibited rapid, simple, and sensitive APE1 detection and showed potential therapeutic applications for cancer treatment.
MICROCHEMICAL JOURNAL
(2023)
Article
Immunology
Gerco den Hartog, Lindsay D. Butcher, Amber L. Ablack, Laura A. Pace, Jailal N. G. Ablack, Richard Xiong, Soumita Das, Thaddeus S. Stappenbeck, Lars Eckmann, Peter B. Ernst, Sheila E. Crowe
Summary: APE1 negatively regulates Rac1 to inhibit invasive bacteria from entering human intestinal epithelial cells, protecting the barrier function of epithelial cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Svetlana Senchurova, Aleksandra A. Kuznetsova, Alexander A. Ishchenko, Murat Saparbaev, Olga S. Fedorova, Nikita A. Kuznetsov
Summary: Escherichia coli apurinic/apyrimidinic (AP) endonuclease Nfo is an important enzyme involved in DNA repair. It recognizes and cleaves AP sites in DNA, which can arise from various sources such as spontaneous hydrolysis or the action of DNA glycosylases. Nfo also has other repair activities, including nucleotide incision repair. This study analyzed the kinetics of Nfo's interaction with DNA substrates and provided insights into the mechanism and rate of nucleotide cleavage. The study also found that 2'-deoxynucleoside monophosphates effectively inhibit Nfo's 3'-5'-exonuclease activity.
Article
Dermatology
Kyung-Il Kim, Kyung Eun Jung, Young-Bin Shin, Chang-Deok Kim, Tae-Jin Yoon
Summary: In this study, we conducted a large-scale screening test on drugs approved for other diseases to find pigmentation-modulating agents. Among the potential drugs, sorafenib was selected for further investigation on its effect on pigmentation in melanoma cells. The results showed that sorafenib promoted pigmentation by increasing the melanin content and tyrosinase activity. Mechanistic studies revealed that this effect was mediated by the activation of beta-catenin signaling through the regulation of GSK3 beta phosphorylation.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu-Ran Lee, Eun Young Bae, Hong Ryang Kil, Byeong-Hwa Jeon, Geena Kim
Summary: This study investigated the association between APE1/Ref-1 and Kawasaki disease (KD). The results showed that APE1/Ref-1 levels were significantly higher in KD patients, especially in refractory KD. APE1/Ref-1 could be a beneficial biomarker for the diagnosis and prognosis of KD.
Article
Biochemistry & Molecular Biology
Su-jeong Choi, Harsha Nagar, Jun Wan Lee, Seonhee Kim, Ikjun Lee, Shuyu Piao, Byeong Hwa Jeon, Cuk-Seong Kim
Summary: This study found that IDH2 deficiency induced increased expression of SDC2 in endothelial cells, with MMP7 and TGF-beta signaling playing regulatory roles. UTI could reverse the increase in SDC2, MMP7, and TGF-beta signaling caused by IDH2 deficiency.
Article
Biochemistry & Molecular Biology
Sunga Choi, Yu-Ran Lee, Ki-Mo Kim, Euna Choi, Byeong-Hwa Jeon
Summary: The regulation of inflammatory signaling in the tumor microenvironment using adenoviral-mediated PPTLS-APE1/Ref-1 can inhibit the activity of inflammatory immune cells and cancer cells, providing a potential therapeutic strategy for treating triple-negative breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Sang Min Jeon, Young Jae Kim, Thanh Quang Nguyen, Jinsheng Cui, Bui Thi Bich Hanh, Prashanta Silwal, Jin Kyung Kim, Jin-Man Kim, Dong-Chan Oh, Jichan Jang, Eun-Kyeong Jo
Summary: The newly identified cyclic peptide Ohmyungsamycin A (OMS) exhibits significant antimicrobial effects against Mycobacterium tuberculosis and has shown promising results against non-tuberculous mycobacteria (NTMs) infections as well. The study demonstrates that OMS treatment enhances the immune response and promotes proinflammatory reactions against Mycobacteroides abscessus (Mabc) infection in both immunocompetent and immunodeficient mice. Furthermore, the combination of OMS and rifabutin shows a synergistic effect against Mabc infections, suggesting the potential of OMS as an adjunctive therapeutic strategy.
Article
Dermatology
Jung-Min Shin, Kyung Min Kim, Chang Deok Kim, Young Lee, Sanghyun Park
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Dermatology
Jung-Min Shin, Kyung Min Kim, Mi Soo Choi, Sanghyun Park, Dongkyun Hong, Kyung-Eun Jung, Young-Joon Seo, Chang Deok Kim, Hanseul Yang, Young Lee
Summary: Alopecia areata (AA) is a T-cell-mediated autoimmune disease causing chronic hair loss, with its exact pathogenesis still unclear. Recent studies have shown the importance of crosstalk between inflammasomes and mitophagy, a process that removes damaged mitochondria. This study found mitochondrial DNA damage in AA-affected scalp tissues and treated cells, as well as increased mitochondrial reactive oxygen species (ROS) levels with treatment. Mitophagy induction was shown to alleviate NLRP3 inflammasome activation, and PINK1-mediated mitophagy played a critical role in inflammasome activation. These findings highlight the significance of mitophagy-inflammasome crosstalk in AA pathogenesis and suggest potential therapeutic targets.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eun-Ok Lee, Hee-Kyoung Joo, Yu-Ran Lee, Sungmin Kim, Kwon-Ho Lee, Sang-Do Lee, Byeong-Hwa Jeon
Summary: Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is involved in DNA repair and redox regulation. It inhibits adipocyte differentiation by regulating adipogenic transcription factors, suggesting it as a potential therapeutic target for regulating adipocyte differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shuyu Piao, Ikjun Lee, Seonhee Kim, Hyewon Park, Harsha Nagar, Su-Jeong Choi, Giang-Huong Vu, Minsoo Kim, Eun-Ok Lee, Byeong-Hwa Jeon, Dong Woon Kim, Youngduk Seo, Cuk-Seong Kim
Summary: This study investigates the role of CRIF1 deficiency in modulating mitochondrial oxidative phosphorylation (OXPHOS) system dysfunction and its antitumor effects in MCF-7 breast cancer cells. CRIF1 silencing decreases the assembly of mitochondrial OXPHOS complexes, leading to mitochondrial dysfunction and elevated reactive oxygen species (ROS) levels. Inhibition of CRIF1 suppresses cell proliferation and inhibits cell migration in MCF-7 cells, and intratumoral injection of CRIF1 siRNA nanoparticles inhibits tumor growth in MCF-7 xenograft mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Young Jae Kim, Eun-Jin Park, Sang-Hee Lee, Prashanta Silwal, Jin Kyung Kim, Jeong Seong Yang, Jake Whang, Jichan Jang, Jin-Man Kim, Eun-Kyeong Jo
Summary: In this study, dimethyl itaconate (DMI) was found to have potential as a candidate for host-directed therapy (HDT) against both Mycobacterium tuberculosis (Mtb) and nontuberculous mycobacteria. It can activate multiple immune defense mechanisms in the host to promote intracellular killing of mycobacteria and suppress inflammation. This research contributes to the development of new anti-tuberculosis drugs.
CELL AND BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ikjun Lee, Shuyu Piao, Seonhee Kim, Harsha Nagar, Su-jeong Choi, Minsoo Kim, Giang-Huong Vu, Byeong-Hwa Jeon, Cuk-Seong Kim
Summary: Endothelial senescence impairs vascular function and is a major factor in age-related vasculature diseases. IDH2 is involved in the production of alpha-ketoglutarate and preservation of mitochondrial function. However, the mechanism and regulation of IDH2 in endothelial senescence have not been fully understood.
Article
Dermatology
Kyung-Il Kim, Seung-Mee Kim, Young-Yoon Lee, Young Lee, Chang-Deok Kim, Tae-Jin Yoon
Summary: This study investigates the action mechanisms of the cholesterol-lowering drug pitavastatin on cutaneous squamous cell carcinoma (SCC) cells. The results show that pitavastatin dose-dependently induces apoptosis of cutaneous SCC cells, but has no effect on normal keratinocytes. Further experiments reveal that pitavastatin-induced apoptosis is achieved through GGPP-dependent JNK activation.
ANNALS OF DERMATOLOGY
(2023)
Article
Dermatology
Sang-Hoon Lee, Hee-Seok Seo, Seong Jun Seo, Chang-Deok Kim, Seung-Phil Hong
Summary: This study investigated the potential of plant extracts to enhance skin-barrier function by promoting lipid synthesis in keratinocytes. The results showed that Melia toosendan extract had the highest expression of lipid synthase and could improve skin-barrier function.
ANNALS OF DERMATOLOGY
(2022)
Article
Dermatology
Changhyeon Kim, Jung-Min Shin, Doyeon Kim, Sanghyun Park, Dongkyun Hong, Kyung Eun Jung, Chang-Deok Kim, Young-Joon Seo, Young Lee
Summary: This study investigates the role of substance P (SP) in the pathogenesis of alopecia areata (AA), finding that SP may contribute to AA by triggering localized inflammation, provoking inflammatory response, and inhibiting hair growth.
ANNALS OF DERMATOLOGY
(2022)
Article
Dermatology
Kyung-Il Kim, Chang-Il Kwon, Jeung-Hoon Lee, Chang-Deok Kim, Tae-Jin Yoon
Summary: This study found that mitoxantrone reduces collagen synthesis by inhibiting TGF-(3/SMAD signaling and LARP6 expression in fibroblasts.
ANNALS OF DERMATOLOGY
(2022)