Article
Ecology
Pawel Markwitz, Cedric Lood, Tomasz Olszak, Vera van Noort, Rob Lavigne, Zuzanna Drulis-Kawa
Summary: The study found that phage-resistance emergence in bacterial populations had a key ecological impact and could be influenced by self and cross-resistance mechanisms. Using seven phages with distinct receptors to infect five Pseudomonas aeruginosa strains, significant differences were observed in resistance mechanisms between strains, which were correlated with bacterial growth rates.
Review
Immunology
Pamella Constantino-Teles, Albane Jouault, Lhousseine Touqui, Alessandra Mattos Saliba
Summary: Pseudomonas aeruginosa, an opportunistic pathogen, causes respiratory infections by utilizing various virulence factors, such as bacterial lipids and enzymes, and by interacting with host lipids. These mechanisms play important roles in the establishment and persistence of P. aeruginosa in the lungs, as well as in modulating host immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Vanna Imsirovic, Maja Lenartic, Felix M. M. Wensveen, Bojan Polic, Vedrana Jelencic
Summary: Natural killer (NK) cells, important in early defense against tumors and virally infected cells, are regulated by a balance of activating and inhibitory receptors. However, some activating receptors lack an inhibitory partner. This study shows that activating NK cell receptors without inhibitory partners are not a "master" signal, but instead their activity is controlled through regulation of other activating receptors. This finding has potential implications for the development of NK cell-based cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Microbiology
Alexandra Grote, Ashlee M. Earl
Summary: Many bacterial pathogens can form persistent infections, and high-throughput sequencing methods have enabled the study of their genetic evolution, revealing common trends in bacterial adaptation to persistent infections.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Haider H. Dar, Tamil S. Anthonymuthu, Liubov A. Ponomareva, Austin B. Souryavong, Galina V. Shurin, Alexandr O. Kapralov, Vladimir A. Tyurin, Janet S. Lee, Rama K. Mallampalli, Sally E. Wenzel, Huelya Bayir, Valerian E. Kagan
Summary: The research found that Pseudomonas aeruginosa degrades host GPx4 defense through activating lysosomal chaperone-mediated autophagy, leading to the activation of the host's anti-ferroptotic mechanism driven by iNOS/NO'. Macrophage-produced NO' can distantly prevent P. aeruginosa-induced ferroptosis in epithelial cells by suppressing phospholipid peroxidation.
Article
Multidisciplinary Sciences
Camilla D. de Mattos, Dominick R. Faith, Artem A. Nemudryi, Amelia K. Schmidt, DeAnna C. Bublitz, Lauren Hammond, Margie A. Kinnersley, Caleb M. Schwartzkopf, Autumn J. Robinson, Alex Joyce, Lia A. Michaels, Robert S. Brzozowski, Alison Coluccio, Denghui David Xing, Jumpei Uchiyama, Laura K. Jennings, Prahathees Eswara, Blake Wiedenheft, Patrick R. Secor
Summary: This study reveals how Pseudomonas aeruginosa assesses threats of cellular injury by using polyamines released by dying cells and linear DNA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Xuerui Bao, Ellen Goeteyn, Aurelie Crabbe, Tom Coenye
Summary: D,L-malate enhances ciprofloxacin activity against P. aeruginosa, improving antibiotic therapy in infection environments. The choice of model system influences the outcome of the experiment, emphasizing the importance of using models that closely mimic the in vivo environment. This study confirms the potential of D,L-malate to enhance ciprofloxacin activity against P. aeruginosa-associated infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Immunology
Dusik Kim, Jie Liao, Nathan B. Scales, Carolina Martini, Xiaojie Luan, Asmahan Abu-Arish, Renaud Robert, Yishan Luo, Geoffrey A. McKay, Dao Nguyen, Marc A. Tewfik, Charles D. Poirier, Elias Matouk, Juan P. Ianowski, Saul Frenkiel, John W. Hanrahan
Summary: The airway surface undergoes large pH excursions during breathing, reaching pH 9.0 during inhalation. Reduced luminal bicarbonate and CA12 expression in cystic fibrosis lead to impaired bacterial-killing efficacy. Defective pH oscillations may compromise airway host defense in other respiratory diseases.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Chemistry, Medicinal
Meghan K. O'Leary, Asraa Ahmed, Christopher A. Alabi
Summary: Novel antimicrobial agents are needed to treat infections caused by multidrug-resistant extracellular pathogens. Antibody-drug conjugates have shown success in delivering highly cytotoxic drugs directly to target cells, and this strategy can be applied to the treatment of infections by utilizing an antibody to deliver an antimicrobial agent.
ACS INFECTIOUS DISEASES
(2023)
Article
Chemistry, Medicinal
Meghan K. O'Leary, Christopher A. Alabi, Asraa Ahmed
Summary: The development of an antibody-bactericide conjugate (ABC) that targets the antimicrobial peptide oligoTEA to treat infections caused by multidrug-resistant extracellular pathogens such as Pseudomonas aeruginosa is proposed. The ABC binds to the bacterial cell surface and releases the oligoTEA payload, exhibiting antipseudomonal activity.
ACS INFECTIOUS DISEASES
(2023)
Review
Infectious Diseases
Guillermo Santamaria-Corral, Abrar Senhaji-Kacha, Antonio Broncano-Lavado, Jaime Esteban, Meritxell Garcia-Quintanilla
Summary: Phage therapy is an alternative therapy used as a last resort against multidrug-resistant bacteria infections. Pseudomonas aeruginosa, a pathogen that commonly infects immunocompromised individuals, can be effectively treated using bacteriophages alone or in combination with antibiotics. This review summarizes recent studies highlighting the synergistic effects of phage-antibiotic combinations and successful treatments.
Article
Immunology
Sannette C. Hall, Deandra R. Smith, Shetty Ravi Dyavar, Todd A. Wyatt, Derrick R. Samuelson, Kristina L. Bailey, Daren L. Knoell
Summary: Zinc is essential for immune function and host defense, regulated by zinc transporters. Zinc deficiency can increase susceptibility to bacterial infections, especially Streptococcus pneumoniae. Myeloid cells, such as macrophages and dendritic cells, play a critical role in shaping the immune response in the lung against bacterial pathogens.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Ecology
Lydia-Ann J. Ghuneim, Ruma Raghuvanshi, Kerri A. Neugebauer, Douglas V. Guzior, Martin H. Christian, Bella Schena, Jeremiah M. Feiner, Alicia Castillo-Bahena, Jenna Mielke, Marc McClelland, Douglas Conrad, Isaac Klapper, Tianyu Zhang, Robert A. Quinn
Summary: This study investigates the effect of antibiotics on host-associated microbial communities using cultured sputum samples from cystic fibrosis patients. The researchers found that in addition to the predicted outcomes, antibiotic treatment can also lead to shifts in community composition and an increase in total bacterial load. A mathematical model reveals that the increase in bacterial load is due to the antibiotic-mediated release of pathogens by anaerobic fermentation. Metagenomic sequencing of clinical samples further confirms these findings.
Article
Microbiology
Manuel Gonzalez de Aledo, Lucia Blasco, Maria Lopez, Concha Ortiz-Cartagena, Ines Bleriot, Olga Pacios, Marta Hernandez-Garcia, Rafael Canton, Maria Tomas
Summary: This research aims to study the nature, composition, and role of prophages found within a set of circulating strains of Pseudomonas aeruginosa, with special attention to high-risk clones. Prophages have the ability to influence bacterial pathogenesis, making the basic research of prophages a topic of growing interest. The abundance of viral defense and regulatory proteins within prophage genomes detected in this study emphasizes the importance of characterizing the most frequent prophages in circulating clinical strains and in high-risk clones for potential phage therapy.
Article
Food Science & Technology
Viktoria Lilla Balazs, Lilla Nagy-Radvanyi, Rita Filep, Erika Kerekes, Bela Kocsis, Marianna Kocsis, Agnes Farkas
Summary: The study investigated the antibacterial and anti-biofilm effects of Hungarian black locust, linden, and sunflower honeys against respiratory tract pathogens. Linden honey was found to be the most effective in both antibacterial and anti-biofilm activities, highlighting its potential role in treating respiratory tract infections caused by biofilm-forming bacteria.