Article
Biochemistry & Molecular Biology
Rajkishor Nishad, Dhanunjay Mukhi, Srinivas Kethavath, Sumathi Raviraj, Atreya S. V. Paturi, Manga Motrapu, Sreenivasulu Kurukuti, Anil Kumar Pasupulati
Summary: This study finds that podocytes in diabetic kidney disease (DKD) may contribute to renal inflammation by secreting TNF-alpha and promoting macrophage migration. Inhibiting the action of GH or TNF-alpha in podocytes could be a novel therapeutic approach for treating DKD.
Article
Immunology
Lingtong Huang, Xueling Fang, Xuan Zhang, Weifang Wu, Hangping Yao, Qiang Fang
Summary: The study confirmed the expression of the RON receptor in BMDCs, showing that inhibiting RON affects the ubiquitination levels of MHC II and CD86, leading to decreased levels of these molecules and reducing the ability of DCs to promote T cell proliferation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Feng Qin, Guodong Chen, Kwan Ngok Yu, Miaomiao Yang, Wei Cao, Peizhong Kong, Shengjie Peng, Mingyu Sun, Lili Nie, Wei Han
Summary: In this study, Golgi protein GOLPH3 was found to play a crucial role in the transduction of RIBE. Knocking down GOLPH3 blocked RIBE generation, while re-expression of GOLPH3 rescued the effect. The GOLPH3/ERK/EGR1 signaling axis was identified as the mechanism that regulates the transcription of TNF-alpha and determines its release level.
Article
Biology
Sheetal Uppal, Tingting Liu, Emily Galvan, Fatima Gomez, Tishina Tittley, Eugenia Poliakov, Susan Gentleman, T. Michael Redmond
Summary: RPE65 recognizes and binds to membranes through specific mechanisms, including interaction between residues 107-125 and membranes, as well as palmitoylation at C112. The study reveals the membrane recognition mechanism of RPE65 and the impact of the alpha-helical structure on its function, while also suggesting a novel role for cysteine palmitoylation.
LIFE SCIENCE ALLIANCE
(2022)
Article
Neurosciences
Shveta V. Parekh, Jacqueline E. Paniccia, Lydia O. Adams, Donald T. Lysle
Summary: The study reveals that chronic heroin administration and withdrawal can increase fear learning and DH TNF-alpha, while TNF-alpha inhibitor can prevent the development of enhanced fear learning and mitigate withdrawal-induced weight loss.
MOLECULAR NEUROBIOLOGY
(2021)
Review
Immunology
Guangyuan Du, Zherui Xing, Jue Zhou, Can Cui, Chenyuan Liu, Yiping Liu, Zheng Li
Summary: The RLR pathway is a significant pathway in cells for monitoring abnormal RNA, which activates inflammatory effects to combat infections and also participates in cancer development. Various modifications are involved in regulating the RLR pathway in cancer, either enhancing tumor suppression or promoting tumor development. This review summarizes epigenetic, post-transcriptional, and post-translational modifications of the RLR pathway in cancer cells, and provides an overview of therapeutic strategies targeting this pathway in cancers.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Geriatrics & Gerontology
Wei-Ju Lin, Wei-Ju Lee, Li-Ning Peng, Yi-Long Huang, Chien-Yi Tung, Chi-Hung Lin, Ting-Fen Tsai, Liang-Kung Chen
Summary: Tumor necrosis factor (TNF)-alpha is a proinflammatory cytokine involved in sarcopenia, but its short half-life limits its potential as a biomarker. Anti-TNF-alpha, as a natural autoantibody to TNF-alpha, has more stable concentrations and should be a better alternative biomarker for sarcopenia.
EXPERIMENTAL GERONTOLOGY
(2023)
Article
Immunology
Ruby J. Siegel, Anil K. Singh, Paul M. Panipinto, Farheen S. Shaikh, Judy Vinh, Sang U. Han, H. Mark Kenney, Edward M. Schwarz, Cynthia S. Crowson, Sadik A. Khuder, Basil S. Khuder, David A. Fox, Salahuddin Ahmed
Summary: This study identified a potential role of Sulf-2 in the inflammatory response of rheumatoid arthritis (RA). The study found that overexpression of Sulf-2, which is positively correlated with TNF receptor 1 expression, can be reduced to attenuate TNF-alpha-induced synovial inflammation.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fabian Johannes Pflieger, Jacqueline Wolf, Martin Feldotto, Andreas Nockher, Tatjana Wenderoth, Jessica Hernandez, Joachim Roth, Daniela Ott, Christoph Rummel
Summary: This study investigated the potential of sensory circumventricular organs (sCVOs) to produce n-3 and n-6 oxylipins after lipopolysaccharide (LPS) stimulation. The results showed that LPS stimulation increased the release of cytokines and certain oxylipins. However, co-treatment with norepinephrine (NE) did not significantly alter the levels of oxylipins or the changes in TNF alpha levels. These findings suggest that LPS-induced oxylipins may play an important role in immune-to-brain communication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Xuefei Sun, Mingwei Li, Jinghao Ban, Zhidan Li
Summary: The study found that miR-23b mediated the TNF-alpha-inhibited osteogenic differentiation of hPDLSCs by targeting Runx2, providing new insights on the expanded function of miR-23b in osteogenesis of hPDLSCs under inflammatory conditions.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Immunology
Jing Huang, Lan Wang, Chuanfei Yu, Zhihao Fu, Chunyu Liu, Gang Wu, Luyun Guo, Xiao Guo, Shiyu Chen, Xumei Liu, Junzhi Wang
Summary: The developed U937 cell-based bioassay for measuring the biological activities of mAbs and biosimilars against TNF-α showed accuracy and precision in determining potency values, making it suitable for quality control and release of mAbs, as well as evaluating the similarity in biological activity of biosimilars.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Catherine F. Hatton, Rachel A. Botting, Maria Emilia Duenas, Iram J. Haq, Bernard Verdon, Benjamin J. Thompson, Jarmila Stremenova Spegarova, Florian Gothe, Emily Stephenson, Aaron I. Gardner, Sandra Murphy, Jonathan Scott, James P. Garnett, Sean Carrie, Jason Powell, C. M. Anjam Khan, Lei Huang, Rafiqul Hussain, Jonathan Coxhead, Tracey Davey, A. John Simpson, Muzlifah Haniffa, Sophie Hambleton, Malcolm Brodlie, Chris Ward, Matthias Trost, Gary Reynolds, Christopher J. A. Duncan
Summary: The innate immune response in epithelial cells following SARS-CoV-2 infection remains unclear. Research using human air-liquid interface culture suggests delayed type I interferon responses compared to other respiratory viruses. The nasal epithelium serves as a likely entry point for virus, and initiating host response. Antiviral interferon responses are crucial in combating SARS-CoV-2, with nasally delivered recombinant IFNs possibly a preventive strategy.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
H. S. Jeffrey Man, Noeline Subramaniam, Tiana Downs, Aravin N. Sukumar, Aninda D. Saha, Ranju Nair, Lucy Chen, Daniel Teitelbaum, Paul J. Turgeon, Kyung Ha Ku, Eileen Tran, Marc de Perrot, Philip A. Marsden
Summary: A novel EC-enriched lncRNA, GATA2-AS1, was discovered to be involved in the physiologic adaptation and angiogenesis of vascular endothelial cells. It regulates HIF1-alpha induction and mitochondrial biogenesis in acute hypoxia. Additionally, GATA2-AS1 plays a role in angiogenesis and is perturbed in atherosclerotic disease.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Ophthalmology
Min Ji, Qing Sun, Guowei Zhang, Zeyu Huang, Yujian Zhang, Qianyi Shen, Huaijin Guan
Summary: The study revealed that microglia-derived TNF-α plays a crucial role in regulating the activation of Muller cells during retinal neurodegeneration. TNF-α led to increased expression of GFAP and proinflammatory factors in Muller cells, while anti-TNFR1 and NF-κB inhibitor could reverse these changes.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Chemistry, Applied
Shaymaa M. Mohamed, Emad H. M. Hassanein, Samir A. Ross, Nesma M. Mohamed
Summary: This study identified several compounds with antioxidant and anti-inflammatory potentials in Adenanthera pavonina, including a previously undescribed compound that showed the strongest inhibition of TNF-alpha production.
NATURAL PRODUCT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
J. Wang, J. Zhao, X. Cui, B. A. Myson, S. Navneet, A. Saul, M. Ahuja, N. Lambert, I. G. Gazaryan, B. Thomas, K. E. Bollinger, S. B. Smith
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Chemistry, Medicinal
Matthew R. Porter, Haiyan Xiao, Jing Wang, Sylvia B. Smith, Joseph J. Topczewski
ACS MEDICINAL CHEMISTRY LETTERS
(2019)
Article
Neurosciences
Yebo Gu, Zhou Wu, Fan Zeng, Muzhou Jiang, Jessica L. Teeling, Junjun Ni, Ichiro Takahashi
JOURNAL OF ALZHEIMERS DISEASE
(2020)
Article
Clinical Neurology
Jay Amin, Delphine Boche, Zoe Clough, Jessica Teeling, Anthony Williams, Yifang Gao, Lindsey Chudley, Laurie Lau, Florence Smith, Scott Harris, Clive Holmes
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2020)
Article
Ophthalmology
Jing Wang, Haiyan Xiao, Shannon Barwick, Yutao Liu, Sylvia B. Smith
Summary: Delayed administration of (+)-PTZ beyond the onset of photoreceptor cell death in rd10 mice can negatively impact the long-term preservation of retinal function. Optimal timing of Sig1R ligand administration is critical for retinal neuroprotection, as revealed by our findings.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Shannon R. Barwick, Mevish S. Siddiq, Jing Wang, Haiyan Xiao, Brendan Marshall, Elizabeth Perry, Sylvia B. Smith
Summary: Sigma 1 receptor (Sig1R) has emerged as a novel target for retinal degenerative disease, mediating retinal neuroprotection by forming a complex with NRF2 and regulating antioxidant response. Studies have shown that Sig1R and NRF2 are within 40 nm of each other, indicating their interaction in cone cells. Additionally, Sig1R can normalize NRF2 levels to modulate antioxidant response.
Article
Ophthalmology
Haiyan Xiao, Jing Wang, Shannon R. Barwick, Yisang Yoon, Sylvia B. Smith
Summary: The study comprehensively analyzed retinal function and structure in 20-month-old Cbs(+/-) and Cbs(+/+) mice and found that chronic, moderate Hhcy elevation did not lead to significant visual function loss or retinal pathology. Compared to age-matched WT mice, there were no major differences in retinal structure or function in the mice with elevated Hhcy levels.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Zhifen Zhou, Yujing Li, Huimin Xu, Xiaowei Xie, Zibin He, Song Lin, Ruofei Li, Shouheng Jin, Jun Cui, Hai Hu, Feng Liu, Su Wu, Wenbin Ma, Zhou Songyang
Summary: This study identified multiple positive regulators, including the E3 ligase DTX2, through an inducible CRISPR/Cas9 KO screen. Depletion of DTX2 in telomerase-positive cancer cells resulted in downregulation of hTERT transcription and telomerase activity, leading to progressive telomere shortening, growth arrest, and increased apoptosis. BioID analysis revealed that DTX2 interacts with multiple transcription factors, with NFIC functioning cooperatively with DTX2 to promote hTERT transcription. Further investigation demonstrated that DTX2 mediates K63-linked ubiquitination of NFIC, which enhances NFIC binding to the hTERT promoter and increases hTERT expression.
Article
Ophthalmology
Shannon R. Barwick, Haiyan Xiao, David Wolff, Jing Wang, Elizabeth Perry, Brendan Marshall, Sylvia B. Smith
Summary: Retinitis pigmentosa (RP) is a devastating inherited retinal disease that leads to visual impairment and blindness. Currently, there is no cure for RP, highlighting the importance of research into prolonging vision. This study investigated the effects of Sigma 1 receptor (Sig1R) activation in a mouse model of RP and found that it can protect visual function and structure. These results provide a framework for further studies on Sig1R as a potential therapeutic target for retinal degenerative diseases.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Cell & Tissue Engineering
Ran Feng, Su Wu, Ruofei Li, Kunling Huang, Ting Zeng, Zhifen Zhou, Xiaoqin Zhong, Zhou Songyang, Feng Liu
Summary: This study used kl/kl mice to investigate the functional change of bone marrow-derived mesenchymal stem cells (BMSCs) and found that klotho deficiency leads to bone abnormalities. The researchers also discovered that kl/kl BMSCs exhibited hyperactive proliferation but functionally declined, possibly due to mTORC1 activation and decreased autophagy. Additionally, they found that the administration of soluble Klotho protein and mTORC1 inhibitor rapamycin restored BMSC quiescence, improved bone phenotype, and increased lifespan in kl/kl mice in vivo.
STEM CELLS AND DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Jing Wang, Shannon R. Barwick, Haiyan Xiao, Sylvia B. Smith
Summary: Sigma 1 receptor (Sig1R) activation is neuroprotective in retinal degeneration models, and the molecular mechanisms involve the antioxidant pathway mediated by the transcription factor Nrf2. This study demonstrates that Sig1R interacts with Cul3, a component of the Nrf2-Keap1 antioxidant pathway, and activation of Sig1R increases Cul3 levels, leading to preserved mitochondrial function and reduced oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Cell Biology
Luming Guo, Xiaoling Xie, Jing Wang, Haiyan Xiao, Shuchun Li, Mei Xu, Ebenezer Quainoo, Rithwik Koppaka, Jiaping Zhuo, Sylvia B. Smith, Lin Gan
Summary: The generation and characterization of a knock-in mouse line, Rbpms-CreER(T2), that allows for the conditional activation of a reporter gene in retinal ganglion cells (RGCs) is reported. The knock-in mice showed efficient recombination and no detectable defects, making them a powerful tool for studying RGCs.
Article
Ophthalmology
Jing Wang, Haiyan Xiao, Shannon R. Barwick, Sylvia B. Smith
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Article
Clinical Neurology
Patrick Garland, Matthew J. Morton, William Haskins, Ardalan Zolnourian, Andrew Durnford, Ben Gaastra, Jamie Toombs, Amanda J. Heslegrave, John More, Azubuike Okemefuna, Jessica L. Teeling, Jonas H. Graversen, Henrik Zetterberg, Soren K. Moestrup, Diederik O. Bulters, Ian Galea
BRAIN COMMUNICATIONS
(2020)
Article
Ophthalmology
Haiyan Xiao, Jing Wang, Alan Saul, Sylvia B. Smith
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
