Article
Clinical Neurology
Jan Kolcava, Monika Hulova, Lucie Rihova, Josef Bednarik, Pavel Stourac
Summary: This study evaluates the impact of natalizumab treatment on lymphocyte subsets, CD4/CD8 ratio, and their relationship with JCV index and clinical data including NEDA-3 and ARR. The results show significant increases in lymphocytosis after natalizumab treatment, with CD4/CD8 ratio correlating with ARR. Patients maintaining NEDA-3 status exhibit lower CD4/CD8 ratio over the treatment period.
NEUROLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tina Petrogiannis-Haliotis, Kevin Pehr, David Roberge, Ryan N. Rys, Yury Monczak, Gizelle Popradi, Lissa Ajjamada, Naciba Benlimame, Christiane Querfeld, Nathalie Johnson, Hans Knecht
Summary: We present a case of CD4+ small/medium-sized pleomorphic lymphoma with extensive lesions in the legs. Immunohistochemistry and flow cytometry analysis showed distinct CD4+ and CD8+ T-cell populations with no CD4/CD8 co-expression. TCR gene rearrangement studies confirmed the neoplastic population to be of T-cytotoxic cell lineage, representing a previously undescribed type of CTCL.
Article
Medicine, General & Internal
Hadrian Platzer, Richard Trauth, Timo A. Nees, Elena Tripel, Simone Gantz, Marcus Schiltenwolf, Babak Moradi, Nils Rosshirt
Summary: This study aimed to analyze the presence and polarization of CD8(+) T cell subsets in osteoarthritis (OA) knee joints in relation to disease stage and compartment. The study found that CD8(+) T cells can differentiate into pro-inflammatory and anti-inflammatory subsets and were detected in both the synovial membrane and synovial fluid. The proportion of CD8(+) T cell subsets varied depending on OA stage and compartment. The findings provide insights into the pathological mechanisms of OA progression and may contribute to the development of novel anti-inflammatory strategies.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Immunology
Hyun Yang, Ji Won Han, Jae Jun Lee, Ahlim Lee, Sung Woo Cho, Pu Reun Rho, Min-Woo Kang, Jeong Won Jang, Eun Sun Jung, Jong Young Choi, Pil Soo Sung, Si Hyun Bae
Summary: This study investigated the phenotypes and clinical implications of liver-infiltrating immune cells in idiosyncratic drug-induced liver injury (DILI). The results showed that activated CD8+ T cells and mononuclear phagocytes are associated with drug-induced liver injury. Therefore, steroids are suggested as a potential treatment option for idiosyncratic DILI.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Rheumatology
Phuong Nguyen, Marc Melzer, Felix Beck, Marco Krasselt, Olga Seifert, Matthias Pierer, Kathrin Rothe, Ulf Wagner
Summary: Expansion of CD4(+)CD8(+) double-positive (DP) T cells in a subset of RA patients is associated with age and erosive joint destruction, leading to more severe joint damage and frequent escalation of therapy. This suggests a potential contribution of DP T cells to the progression of more severe RA.
Article
Hematology
Devy Deliyanti, William A. Figgett, Thomas Gebhardt, Joseph A. Trapani, Fabienne Mackay, Jennifer L. Wilkinson-Berka
Summary: CD8(+) T cells contribute to pathological angiogenesis by migrating into the retina and releasing cytokines and cytotoxic factors. CXCR3 is identified as the key pathway for the migration of CD8(+) T cells into the retina, and blocking CXCR3 reduces the number of CD8(+) T cells and retinal vasculopathy. This research reveals the important role of CD8(+) T cells in retinal inflammation and vascular disease, and reducing CD8(+) T cells could be a potential treatment for neovascular retinopathies.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Medicine, General & Internal
Fernando Fernandez-Banares, Natalia Lopez-Palacios, Maria Corzo, Beatriz Arau, Mercedes Rubio, Marta Fernandez-Prieto, Eva Tristan, Mar Pujals, Sergio Farrais, Saul Horta, Juana Maria Hernandez, Marta Gomez-Perosanz, Pedro A. Reche, Maria Esteve, Concepcion Nunez
Summary: The study found significant differences in the percentage of CD8(+) and TCR gamma delta(+) cell subsets between CD patients and non-CD subjects after a 3-day gluten challenge. Measuring activated CD8(+) T cells provided highly accurate CD diagnosis with 95% specificity and 97% sensitivity, similar to IFN-gamma ELISPOT results.
Review
Immunology
Zicheng Hu, Sanchita Bhattacharya, Atul J. Butte
Summary: Modern cytometry technologies allow profiling of the immune system at a single-cell resolution with over 50 protein markers, and the number of publicly available cytometry datasets is increasing. Analyzing cytometry data remains challenging due to its high dimensionality, large cell numbers, and dataset heterogeneity, but machine learning techniques are well suited for addressing these challenges and have been employed in various aspects of cytometry data analysis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Daixi Jiang, Can Chen, Danying Yan, Xiaobao Zhang, Xiaoxiao Liu, Dong Yan, Dawei Cui, Shigui Yang
Summary: Exhausted CD8(+) T cells in HBV carriers exhibit higher levels of inhibitory receptors and enhanced fatigue phenotype in specific subsets. Moreover, CD8(+) T cells in HBV carriers produce lower levels of cytokines, and there are differences in cytokine production levels among different subsets.
Article
Biochemistry & Molecular Biology
Amelie Rousseliere, Laurence Delbos, Celine Bressollette, Mailys Berthaume, Beatrice Charreau
Summary: This study quantitatively determined the frequency of various anti-HCMV immune cell subsets and qualitatively analyzed their phenotypes using spectral flow cytometry analysis. The results provide insights into the cellular immune responses to HCMV infection or vaccines, and can be used for monitoring and analysis of T-cell and NK cell responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Laura J. Pallett, Leo Swadling, Mariana Diniz, Alexander A. A. Maini, Marius Schwabenland, Adria Dalmau Gasull, Jessica Davies, Stephanie Kucykowicz, Jessica K. K. Skelton, Niclas Thomas, Nathalie M. M. Schmidt, Oliver E. E. Amin, Upkar S. S. Gill, Kerstin A. A. Stegmann, Alice R. R. Burton, Emily Stephenson, Gary Reynolds, Matt Whelan, Jenifer Sanchez, Roel de Maeyer, Clare Thakker, Kornelija Suveizdyte, Imran Uddin, Ana M. M. Ortega-Prieto, Charlotte Grant, Farid Froghi, Giuseppe Fusai, Sabela Lens, Sofia Perez-del-Pulgar, Walid Al-Akkad, Giuseppe Mazza, Mahdad Noursadeghi, Arne Akbar, Patrick T. F. Kennedy, Brian R. R. Davidson, Marco Prinz, Benjamin M. M. Chain, Muzlifah Haniffa, Derek W. W. Gilroy, Marcus Dorner, Bertram Bengsch, Anna Schurich, Mala K. K. Maini
Summary: CD14(+)CD8(+) T cells in the liver exhibit immunomodulatory features and play a role in liver transplantation, infection, and tumors. These cells have high levels of IL-10 and IL-2 production, as well as enhanced antiviral/anti-tumor effector function. Additionally, these cells can enhance the tumor-killing ability of CD8(+) T cells by acquiring the lipopolysaccharide receptor complex from mononuclear phagocytes. Bacterial products and tissue stromal factors can tune liver immunity by driving myeloid instruction of CD8(+) T cells.
Article
Biochemical Research Methods
Helen Loo Yau, Daniel D. De Carvalho
Summary: CD8+ T cells are key effector cells in the adaptive immune response, and drug treatments may impact their function. This protocol outlines robust methods for measuring the effects of drugs on the activation and cytolytic potential of primary human CD8+ T cells through ex vivo polyclonal activation and cell culture conditions.
Article
Multidisciplinary Sciences
Kaiwen Wang, Yuqiu Yang, Fangjiang Wu, Bing Song, Xinlei Wang, Tao Wang
Summary: While scRNA-seq data analysis techniques are advanced, research on CyTOF data analysis has lagged behind. Dimension reduction methods were benchmarked on real and synthetic CyTOF samples, highlighting the high level of complementarity between the methods. The study provides useful guidelines for choosing the appropriate method based on data structure and analytical needs.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Huw D. Summers, Carla P. Gomes, Aida Varela-Moreira, Ana P. Spencer, Maria Gomez-Lazaro, Ana P. Pego, Paul Rees
Summary: The study utilized data simulation techniques to model nanoparticle drug delivery processes, revealing the dynamic nature of dose variation over time, including supply time constant and degradation rate constant. The model successfully replicated the dynamic changes in cell-to-cell dose variation.
Article
Biochemistry & Molecular Biology
Maha K. Rahim, Trine Line H. Okholm, Kyle B. Jones, Elizabeth E. McCarthy, Candace C. Liu, Jacqueline L. Yee, Stanley J. Tamaki, Diana M. Marquez, Iliana Tenvooren, Katherine Wai, Alexander Cheung, Brittany R. Davidson, Vrinda Johri, Bushra Samad, William E. O'Gorman, Matthew F. Krummel, Annemieke van Zante, Alexis J. Combes, Michael Angelo, Lawrence Fong, Alain P. Algazi, Patrick Ha, Matthew H. Spitzer
Summary: CD8+ T cell responses in lymph nodes play a critical role in anti-tumor immunity in human head and neck squamous cell carcinomas. These responses are associated with specific T cells in circulation and provide important insights for immune-based therapies in cancer.