期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 400, 期 -, 页码 23-29出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2013.06.013
关键词
Mouse; Lymph node; Lymphatic drainage; India ink; Magnetic resonance imaging
资金
- ACS [SP0011492]
- NCI [CA134719]
- NIH Clinical and Translational Science Awards Program (Northwestern University) [UL1RR0254741]
- National Natural Science Foundation of China [NSFC81220108011]
Mouse models are fundamental to the study and design of new techniques for the cancer diagnosis and treatment. The lymphatic system plays an active role in oncogenesis and metastatic disease progression. However, the in vivo identification of LNs in mice is challenging with conventional imaging modalities since the LN diameter in normal mice is 1-2 mm. Standard dissection techniques are challenging and can only provide endpoint data. Here, we describe high resolution MRI (HR-MRI) approaches for the non-invasive detection of mouse LNs in vivo. We compare in vivo non-invasive HR-MRI methods (without exogenous contrast injections) to the ex vivo dye injection methods for the identification of commonly studied LNs in both normal mice and a mouse model of pancreatic ductal adenocarcinoma (PDAC). We demonstrated the potential to use HR-MRI techniques as a non-invasive imaging assay for visualizing mouse LNs in vivo. (C) 2013 Elsevier B.V. All rights reserved.
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