Detection of cardiac myosin heavy chain-α-specific CD4 cells by using MHC class II/IAk tetramers in A/J mice

A/J mice bearing the H-2 allele IA(k) are highly susceptible to autoimmune myocarditis induced with cardiac myosin heavy chain (Myhc)-alpha 334-352, whereas B10.A mice carrying a similar allele IA(k) are relatively resistant, suggesting that the generation of Myhc-alpha-reactive T cell repertoires is influenced by genetic background. To enumerate the precursor frequencies of Myhc-alpha-specific CD4 T cells, we sought to create IA(k) tetramers for Myhc-alpha 334-352. Tetramers were created using approaches that involve covalent tethering of individual peptide sequences or exogenous loading of peptides into empty IA(k) molecules by peptide-exchange reaction. Using ribonuclease 43-56 tetramers as controls, we demonstrated that by flow cytometry (FC). Myhc-alpha 334-352 tetramers specifically bind myosin-reactive T cells. CD4 cells isolated from A/J mice immunized with Myhc-a 334-352 were used to optimize conditions for tetramer staining, and neuraminidase treatment prior to tetramer staining permitted the detection of Myhc-alpha-specific cells ex vivo. The reagents are useful tools for monitoring the frequency of Myhc-alpha-reactive CD4 cells and to determine their pathogenic potential at a single cell level by FC. (C) 2011 Elsevier B.V. All rights reserved.