期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 369, 期 1-2, 页码 98-107出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2011.04.012
关键词
ePFT; Pancreas; Biomarker; Pancreatitis; Pancreatic juice; Pancreas fluid
资金
- NIH [1 F32 DK085835-01A1, 1 R21 DK081703-01A2, 5 P30 DK034854-24]
- Burrill family
Cytokines are secreted immunomodulating proteins involved in pancreatic stellate cell activation and propagation of fibrosis in chronic pancreatitis. We aim to show that cytokines can be identified from pancreatic fluid by (1) collecting pancreatic fluid with the ePFT method. (2) processing the fluid for cytokine-targeted microarray analysis, and (3) comparing cytokine profiles in pancreatic fluid of chronic pancreatitis (CP) patients and of chronic abdominal pain (CAP) controls. We endoscopically collected pancreatic fluid from patients with CP and those with CAP using the ePFT method. This fluid was subjected directly to a multiplexed cytokine protein microarray assay. Six patients (3 CP, 3 CAP) underwent a secretin-stimulated ePFT. The mean peak bicarbonate concentrations [meg/L] of the CP and CAP patients were 43 and 97. respectively. Statistically significant decreases in the cytokine concentrations of EGF. IP-10, eotaxin, IL-3, MIP-1a, IL-15, PDGF-AB/BB, and IL-1a were observed in the CP specimens ( p < 0.05). We have successfully identified differences in the abundance of cytokines in ePFT-collected pancreatic fluid with a multiplexed microarray assay comparing CP and CAP controls. Further targeted investigation of cytokines in ePFT-collected fluid will broaden our knowledge of pancreatic immune response and pathogenesis in chronic pancreatitis. (C) 2011 Elsevier B.V. All rights reserved.
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