期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 339, 期 2, 页码 264-269出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2008.09.004
关键词
Human rhinovirus; Primary airway epithelial cells; Mouse model of rhinovirus infection
资金
- NHLBI NIH HHS [P01 HL070831-05, P01 HL070831] Funding Source: Medline
- NIAID NIH HHS [U19 AI070503-02, U19 AI070503] Funding Source: Medline
Human rhinoviruses (HRV) are the primary etiological agents in cold infections, and represent a serious risk to individuals with chronic respiratory disease such as asthma. In order to develop treatment options for HRV infections, murine models are a crucial component in the study of infection mechanisms due to the wide array of reagents and techniques available to study murine immunology. We present here a cell culture system for studying isolated murine epithelial cell responses to HRV. Monolayers of primary mouse airway epithelial cells were maintained in a serial culture system, and the identity and purity of the cell population was confirmed via immunostaining (positive for cytokeratin, negative for vimentin). infection of these cells with a minor group rhinovirus (HRV-1A) was evidenced by increases in viral RNA, de novo synthesis of viral proteins, and production of infectious virus. This model will be useful in experiments to define mechanisms of viral replication and host/virus interactions within airway epithelial cells. (C) 2008 Elsevier B.V. All rights reserved.
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