Article
Veterinary Sciences
Putri Pandarangga, Milton M. McAllister, Anne E. Peaston, Yuen T. Ngai, Mohammad I. Cahyono, Farhid Hemmatzadeh
Summary: Antigenic differences between commercial Newcastle Disease Virus (NDV) vaccine and circulating field virus reduce vaccine efficacy. Superior results are obtained when serological testing, vaccinations, and vaccine challenge experiments all use circulating strains of ND virus.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Oncology
Adam Walter, Rodney P. Rocconi, Bradley J. Monk, Thomas J. Herzog, Luisa Manning, Ernest Bognar, Gladice Wallraven, Phylicia Aaron, Staci Horvath, Min Tang, Laura Stanbery, Robert L. Coleman, John Nemunaitis
Summary: This study provided long-term follow-up of 3 years for HRP patients treated with Vigil, showing prolonged RFS and OS without long-term toxicity, supporting further evaluation of Vigil in HRP ovarian cancer.
GYNECOLOGIC ONCOLOGY
(2021)
Article
Chemistry, Analytical
Jiayan Zhu, Ting Li, Shihui Zhang, Xiaomei Zou, Yingchun Zhou, Weiguo Lu, Zhihui Liu, Tao Deng, Fang Liu
Summary: Horseradish peroxidase (HRP) combined with its fluorescence substrates, such as Amplex red, is widely used in biochemical analysis. However, Amplex red has limitations due to nonspecific responsiveness. In this study, three new dihydrofluorescein derivatives were identified as improved HRP substrates. The most promising derivative, DCFH-1, exhibited excellent sensitivity and selectivity for HRP detection, outperforming Amplex red. DCFH-1 was further used in a fluorescence ELISA for the quantification of inflammatory cytokine biomarkers, yielding successful results.
Article
Biotechnology & Applied Microbiology
Yang Mu, Cunyu Jia, Xu Zheng, Haipeng Zhu, Xin Zhang, Haoran Xu, Baoyuan Liu, Qin Zhao, En-Min Zhou
Summary: A rapid, low-cost, reliable and useful cELISA method based on nanobody technology was developed for detecting PCV2 antibodies. The experiment demonstrated that this method has high sensitivity and specificity, and a high consistency rate with commercial kits.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Zainab Ahmed, Ahmed Tokhi, Mehreen Arif, Naeem Ur Rehman, Vahid Sheibani, Khalid Rauf, Robert D. E. Sewell
Summary: This study investigated the acute effects of fraxetin in a chronic unpredictable stress (CUS) paradigm. The results demonstrated that fraxetin decreased depression-like behavior and anxiety, improved memory deficits, and restored altered neurochemical levels. These findings suggest a potential application of fraxetin in managing anxiety and depression. However, further research is needed to explore the chronic effects of fraxetin.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Materials Science, Biomaterials
Yujing Zeng, Minghui Wang, Zhaowei Sun, Lingjun Sha, Jie Yang, Genxi Li
Summary: This study presents a novel colorimetric immunosensor using 2D-MOF nanomaterials as enzyme mimics for protein analysis. The sensor shows high sensitivity and selectivity in detecting carcinoembryonic antigen (CEA), with the ability to distinguish between colorectal cancer patients and healthy individuals using human serum.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Agriculture, Dairy & Animal Science
Mohammad Afrouziyeh, Martin J. Zuidhof
Summary: The study focused on the correlation between broiler breeder plasma CORT concentrations measured using ELISA and LC-MS/MS methods. The results showed a high correlation between the two methods, validating their interchangeable usage. Furthermore, photostimulation BW and breeders' age did not affect plasma CORT levels, indicating similar welfare status between the high and low BW groups.
Review
Oncology
Rachel Royfman, Emma Whiteley, Olivia Noe, Susan Morand, Justin Creeden, Laura Stanbery, Danae Hamouda, John Nemunaitis
Summary: Mutations in BRCA1 or BRCA2 genes increase the risk of breast and ovarian cancer by impacting DNA repair and homologous recombination. Dysregulation of BRCA1/2 not only drives oncogenesis and cancer proliferation, but also affects immune response, influencing disease progression.
Article
Biochemical Research Methods
Nandini Debnath, Ludovic Saiveng Live, Mahla Poudineh
Summary: Biomarker detection in whole blood is important for understanding the disease. Current detection techniques have limitations, and there is a need for reliable and adaptable methods for point-of-care testing. We have developed a simple and low-cost protein detection device that can detect biomarkers in whole blood within 15 minutes. This technique has the potential to improve the diagnosis and screening of various diseases.
Article
Parasitology
D. Daipert-Garcia, V. G. Virginio, F. B. Oliveira, N. G. Siqueira, H. B. Ferreira, R. Rodrigues-Silva
Summary: Polycystic echinococcosis is a zoonosis endemic in the Neotropical region of the Americas, diagnosed based on clinical and epidemiological features, and imaging techniques. Current serological tests for detecting antibodies against Echinococcus spp. have limitations, thus the need for improvement. Research indicates that using specific antigens like AgB8/1 and AgB8/2 may serve as potential substitutes for the current crude antigens.
JOURNAL OF HELMINTHOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Tianxiang Chen, Baoyuan Liu, Yiyang Chen, Xueting Wang, Meimei Zhang, Xukun Dang, Qin Zhao, En-Min Zhou
Summary: A rapid and reliable competitive ELISA (cELISA) was developed to detect avian HEV antibodies, which showed good specificity. The epitope recognized by Nb49 was located in aa 593-604 of the avian HEV capsid protein, and the peptide TFPS in aa 601-604 was essential for binding.
Article
Public, Environmental & Occupational Health
Jaeeun Yoo, Younjeong Kim, Yu mi Cha, Jaewoong Lee, Yeon Jeong Jeong, Si-Hyun Kim, Lisa L. Maragakis, Seungok Lee
Summary: This study compared the efficacy of three different immunization methods against the wild-type, delta, and omicron variants of SARS-CoV-2. The results showed that heterologous booster vaccination (AZ/AZ/BNT) provided the highest level of protection against the omicron variant, followed closely by COVID-19 patients.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Biotechnology & Applied Microbiology
Chenchit Chayachinda, Kanokwaroon Watananirun, Chayawat Phatihattakorn, Sanitra Anuwutnavin, Suvimol Niyomnaitham, Wanatpreeya Phongsamart, Keswadee Lapphra, Orasri Wittawatmongkol, Supattra Rungmaitree, Laddawan Jansarikit, Kobporn Boonnak, Patimaporn Wongprompitak, Sansnee Senawong, Avishek Upadhya, Zheng Quan Toh, Paul V. V. Licciardi, Kulkanya Chokephaibulkit
Summary: This trial evaluated the immunogenicity and reactogenicity of different COVID-19 vaccination schedules in pregnant Thai women. Heterologous schedules of CoronaVac-BNT162b2 or ChAdOx1-BNT162b2 showed similar immunogenicity to BNT162b2-BNT162b2. Neutralizing antibody levels were highest in the CoronaVac-BNT162b2 group. No significant difference in cord blood antibody levels between groups were observed. Local and systemic adverse events were mild-to-moderate and more frequent in the ChAdOx1-BNT162b2 group.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2023)
Article
Agriculture, Multidisciplinary
Jin Wang, Xing Shen, Peng Zhong, Zhaodong Li, Qiushi Tang, Xinan Huang, Anatoly V. Zherdev, Boris B. Dzantiev, Sergei A. Eremin, Zhili Xiao, Hongtao Lei, Xiangmei Li
Summary: A heterologous immunoassay strategy was designed to enhance the sensitivity for the detection of banned dye RB in fraudulent food in this study. The proposed immunoassay can be used for the surveillance screening of RB in a range of seasoning foods, indicating the heterologous strategy is an effective approach to enhance sensitivity in an immunoassay.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2021)
Article
Biotechnology & Applied Microbiology
Renata Novakova, Dagmar Homerova, Dominika Csolleiova, Bronislava Rezuchova, Beatrica Sevcikova, Rachel Javorova, Lubomira Feckova, Jan Kormanec
Summary: Bacteria of the genus Streptomyces are important producers of biologically active natural products. However, many of these products are silent under laboratory conditions. In this study, we optimized a strain of Streptomyces lavendulae for heterologous gene expression and successfully integrated reporter genes and biosynthetic gene clusters into a vector system. This study provides valuable insights into harnessing the biosynthetic potential of Streptomyces bacteria.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
