Review
Cardiac & Cardiovascular Systems
Alexander Akhmedov, Tatsuya Sawamura, Chu-Huang Chen, Simon Kraler, Daria Vdovenko, Thomas F. Luscher
Summary: LOX-1, as a scavenger receptor, plays a crucial role in the development of atherosclerosis, with implications in various cell types, making it a promising diagnostic and therapeutic target for certain cardiovascular diseases.
EUROPEAN HEART JOURNAL
(2021)
Review
Cardiac & Cardiovascular Systems
Alexander Akhmedov, Tatsuya Sawamura, Chu-Huang Chen, Simon Kraler, Daria Vdovenko, Thomas F. Luescher
Summary: LOX-1, a scavenger receptor that promotes atherosclerotic plaque formation, plays a critical role in the development of cardiovascular diseases. Its expression in various cell types underscores its significance in disease progression, highlighting the potential for LOX-1 as a novel therapeutic target in CVDs prevention and treatment.
EUROPEAN HEART JOURNAL
(2021)
Article
Surgery
Halit Eren Taskin, Ahmet Kocael, Pinar Kocael, Kagan Zengin, Muzaffer Al, Volkan Sozer, J. N. Buchwald, T. W. McGlennon, Hafize Uzun
Summary: The study found that weight loss induced by sleeve gastrectomy (SG) can improve the cardiovascular conditions of patients with morbid obesity. After 12 months of SG, metabolic and inflammatory biomarkers significantly decreased and approached the levels of the control group. Baseline albumin level as well as the reduction in oxLDL and cholesterol retention fraction (CRF) were correlated with the reduction in sLOX-1.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2022)
Article
Food Science & Technology
Qing Li, Xiuping Liu, Xu Zhang, Yushi Du, Guanyu Chen, Panyin Xiang, Wenhua Ling, Dongliang Wang
Summary: Sustained inflammation promotes macrophage foam cell formation by promoting cholesterol influx and impairing cholesterol efflux. Lactucopicrin at physiologically reachable concentrations inhibits oxLDL-induced foam cell formation in inflammatory mouse macrophages. The mechanism involves reducing LOX-1 distribution in lipid rafts, which contributes to its atheroprotective effect.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Oncology
Jihang Yao, Keizo Takenaga, Nobuko Koshikawa, Yuki Kida, Jason Lin, Takayoshi Watanabe, Yoshiaki Maru, Yoshitaka Hippo, Seigi Yamamoto, Yuyan Zhu, Hiroki Nagase
Summary: A study found that a compound CCC-h1005 based on triphenylphosphonium has specific cytotoxic effects on cervical cancer cells and cisplatin-resistant cells, and can inhibit tumor growth in a HeLa tumor model without severe adverse effects. This provides a foundation for clinical trials of this class of molecules for treating cervical cancer and other types of cancers.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Medicine, General & Internal
Sandeep Kumar, Wahid Ali, Sridhar Mishra, Akshyaya Pradhan, Rishi Sethi, Rashmi Kushwaha, Uma Shankar Singh, Marco Alfonso Perrone
Summary: The study revealed that sLOX-1 can serve as a biomarker for stable CAD and ACS, with potential in predicting the risk of future recurrence of coronary artery disease.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Hitomi Ohinata, Takashi Obama, Tomohiko Makiyama, Yuichi Watanabe, Hiroyuki Itabe
Summary: This study found that high-density lipoproteins (HDL) partially decreased neutrophil extracellular trap (NET) formation induced by copper-oxidized low-density lipoprotein (oxLDL). It was also discovered that oxidized phosphatidylcholines and lysophosphatidylcholine in oxLDL promoted NET formation, which could be completely blocked by native HDL. Furthermore, an electronegative subfraction of LDL, LDL(-), was found to promote NET formation. These findings suggest that plasma lipoproteins and their oxidative modifications play multiple roles in promoting NET formation, and HDL acts as a suppressor of this response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Susumu Ikenoshita, Kazuya Matsuo, Yasushi Yabuki, Kosuke Kawakubo, Sefan Asamitsu, Karin Hori, Shingo Usuki, Yuki Hirose, Toshikazu Bando, Kimi Araki, Mitsuharu Ueda, Hiroshi Sugiyama, Norifumi Shioda
Summary: A compound called CWG-cPIP has been found to suppress the pathogenesis of both coding and noncoding CWG repeat diseases by selectively inhibiting pathogenic mRNA transcripts and reducing the accumulation of abnormal RNA and protein levels. This compound has shown promising results in mouse models, ameliorating behavioral deficits without any toxic effects.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Cardiac & Cardiovascular Systems
Sara E. Stinson, Anna E. Jonsson, Mette K. Andersen, Morten A. V. Lund, Louise Aas Holm, Cilius E. Fonvig, Yun Huang, Evelina Stankevic, Helene Baek Juel, Lars aengquist, Thorkild I. A. Sorensen, Emily L. Ongstad, Ranjitha Gaddipati, Joseph Grimsby, Christopher J. Rhodes, Oluf Pedersen, Michael Christiansen, Jens-Christian Holm, Torben Hansen
Summary: This study found that sLOX-1 levels were elevated during and after puberty in children and adolescents with overweight/obesity, and were associated with inflammatory markers and worsened cardiometabolic risk profiles. sLOX-1 may serve as an early marker of cardiometabolic risk and inflammation in pediatric overweight/obesity.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Chemistry, Multidisciplinary
Yuki Hirose, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama
Summary: The study demonstrated that the conjugation of chlorambucil to cyclic PIPs showed higher DNA alkylation activity and cytotoxicity against prostate cancer cells compared to hairpin PIP-chlorambucil conjugates. The results suggest that the novel cPIP-Chbs could be promising DNA alkylating anticancer drugs.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Daigo Funakoshi, Daisuke Obinata, Kyoko Fujiwara, Shinichiro Yamamoto, Kenichi Takayama, Makoto Hara, Satoru Takahashi, Satoshi Inoue
Summary: The study demonstrates that the pyrrole-imidazole polyamide modified with a DNA alkylating agent, chlorambucil (OCT1-PIP-ChB), has a significant inhibitory effect on prostate cancer cell proliferation and castration-resistant tumor growth, suggesting its potential as a therapeutic agent.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Toshimasa Takahashi, Yibin Huang, Koichi Yamamoto, Go Hamano, Akemi Kakino, Fei Kang, Yuki Imaizumi, Hikari Takeshita, Yoichi Nozato, Satoko Nozato, Serina Yokoyama, Motonori Nagasawa, Tatsuo Kawai, Masao Takeda, Taku Fujimoto, Kazuhiro Hongyo, Futoshi Nakagami, Hiroshi Akasaka, Yoichi Takami, Yasushi Takeya, Ken Sugimoto, Herbert Y. Gaisano, Tatsuya Sawamura, Hiromi Rakugi
Summary: The study revealed a previously unidentified mechanism whereby internalization of GPCR mediates cellular endocytosis of a specific PRR ligand. OxLDL activates selective G proteins and beta-arrestin-dependent endocytosis pathway, leading to accumulation in human vascular endothelial cells.
Article
Biochemistry & Molecular Biology
Barnaby W. R. Roper, Christian Tiede, Izma Abdul-Zani, Gary A. Cuthbert, Dhananjay Jade, Ahmed Al-Aufi, William R. Critchley, Queen Saikia, Shervanthi Homer-Vanniasinkam, Tatsuya Sawamura, Michael J. Mcpherson, Michael A. Harrison, Darren C. Tomlinson, Sreenivasan Ponnambalam
Summary: In multicellular organisms, lipid-protein particles regulate the flow of lipids and fatty acids between cells. Oxidation of these particles triggers pathological responses mediated by scavenger receptors. This study identified synthetic proteins, termed Affimers, that specifically bind to oxidized low-density lipoprotein (oxLDL) and inhibit its binding and uptake by cells expressing LOX-1. The findings provide insights into vascular diseases and can contribute to further understanding of related pathologies.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Katharine A. Kott, Elijah Genetzakis, Michael P. Gray, Peter Hansen, Helen M. Mcguire, Jean Y. Yang, Stuart M. Grieve, Stephen T. Vernon, Gemma A. Figtree
Summary: Researchers found that serum sLOX-1 is associated with the severity and incidence of CAD, especially in the population without standard modifiable cardiovascular risk factors (SMuRFs), suggesting that sLOX-1 can be a useful biomarker for risk prediction.
Article
Biochemistry & Molecular Biology
Nobuko Koshikawa, Yuki Kida, Nanami Yasui, Yoshinao Shinozaki, Kohei Tsuji, Takayoshi Watanabe, Jason Lin, Seigi Yamamoto, Keizo Takenaga, Hiroki Nagase
Summary: Somatic mutations in mitochondrial DNA present a potential avenue for cancer therapy, and short linear PI-TPPs demonstrate cytotoxic effects and induction of apoptosis in cells with A3243G mitochondrial mutations through targeted mitochondrial homing.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yuki Mori, Hiroki Oi, Yuki Suzuki, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa
Summary: Ribozymes with modular architecture have been designed, forming closed trimers and closed tetramers based on assembly of unit RNAs (L-RNAs). By tuning their structural elements, closed pentamers and closed hexamers were further extended, with their assembly properties analyzed using electrophoretic mobility shift assay (EMSA) and atomic force microscopy (AFM).
Article
Chemistry, Multidisciplinary
Junya Akagi, Takahiro Yamada, Kumi Hidaka, Yoshihiko Fujita, Hirohide Saito, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa
Summary: Ribozymes are attractive platforms for constructing nanoscale objects with biological functions. In this study, a dimeric form of the Tetrahymena group I ribozyme was designed and analyzed biochemically and observed directly by atomic force microscopy. The formation of the ribozyme dimer triggered trans-splicing reactions, resulting in a fluorescent RNA aptamer as the trans-splicing product.
APPLIED SCIENCES-BASEL
(2021)
Review
Biochemistry & Molecular Biology
Soumya Sethi, Hiroshi Sugiyama, Masayuki Endo
Summary: DNA nanotechnology is a promising toolkit for mimicking cell-cell and cell-matrix interactions, aiding in understanding and manipulating cellular functions. Future innovations in the field can be inspired by exploring the potentials of DNA-based nanostructures and addressing current challenges.
Article
Biochemistry & Molecular Biology
Yuki Hirose, Tomo Ohno, Sefan Asamitsu, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama
Summary: A cyclic pyrrole-imidazole polyamide (cPIP) was synthesized to bind to the abnormal expanded CAG/CTG repeat DNA sequences, showing high binding affinity and specificity for its target DNA sequence.
Article
Biochemistry & Molecular Biology
Ryu Tashiro, Ji Hye Yum, Soyoung Park, Hiroshi Sugiyama
Summary: The study showed that the photoreactivity of U-Br changes dramatically from hydrogen abstraction to cross-linking by changing the conformation of the duplex from the B-form to the A-form. Among three A-form structures, the largest amount of cross-linked products was observed when U-Br was incorporated into the RNA strand and the pyrene was conjugated to the 5 ' end of the DNA. These results indicate that the contact manner of pyrene was different between A- and B-form duplexes.
Article
Biochemistry & Molecular Biology
Kai Yu, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa
Summary: Naturally occurring ribozymes with modular architecture are promising for designing and assembling three-dimensional RNA nanostructures with catalytic abilities. In this study, RNA nanostructures with polygonal-shaped ribozyme oligomers were constructed using unit RNAs from the Tetrahymena group I intron, and ribozyme trimers with a triangular shape were dimerized by introducing pillar units. The resulting double-decker nanostructures containing six ribozyme units showed higher catalytic activity than the parent ribozyme trimers.
Article
Peripheral Vascular Disease
Tomoyasu Otsuki, Noboru Fukuda, Lan Chen, Takahiro Ueno, Masari Otsuki, Masanori Abe
Summary: This study demonstrates that upregulation of TWIST1 in GMCs from SHRs leads to increased expression of the C3 gene, potentially contributing to the synthetic phenotype of mesenchymal tissue in hypertension.
HYPERTENSION RESEARCH
(2022)
Article
Urology & Nephrology
Yasutaka Murata, Daisuke Obinata, Taro Matsumoto, Yuichiro Ikado, Koichiro Kano, Noboru Fukuda, Kenya Yamaguchi, Satoru Takahashi
Summary: This study successfully established a persistence SUI model and demonstrated the therapeutic effects of DFAT cell transplantation for persistence SUI. These findings suggest that DFAT cells may be a cell source for the treatment of SUI.
INTERNATIONAL UROLOGY AND NEPHROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sagun Jonchhe, Shankar Pandey, Christian Beneze, Tomoko Emura, Hiroshi Sugiyama, Masayuki Endo, Hanbin Mao
Summary: Researchers found that a DNA nanobowl significantly increased the stability of a human telomeric G-quadruplex and its binding with a ligand. By minimizing the proximity effect and attributing the increased affinity to the nanoconfinement effect, they provided insights into the mechanisms of binding events in nanocavities.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Vinodh J. Sahayasheela, Zutao Yu, Yuki Hirose, Ganesh N. Pandian, Toshikazu Bando, Hiroshi Sugiyama
Summary: Research suggests that targeting Gli-mediated transcription to inhibit the proliferation of cancer stem cells may be a promising strategy.
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2022)
Article
Biochemistry & Molecular Biology
Shingo Hirashima, Hiroshi Sugiyama, Soyoung Park
Summary: This study characterized 2FA-containing DNA molecules and found that they have similar structure and properties to native adenine due to their small size, but exhibit unique features due to their high electronegativity. This provides valuable information for future applications of 2FA-modified DNA.
Article
Biochemistry & Molecular Biology
Junnosuke Hatanaka, Yuki Hirose, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama
Summary: In this study, we synthesized linear pyrrole-imidazole polyamides (PIPs) that can selectively bind to DNA. These linear PIPs have lower molecular weights compared to commonly used hairpin PIPs. The binding affinity of the PIPs to DNA was improved by modifying the N-terminus of the linear PIPs. These findings provide insights for the design of effective linear PIPs.
Article
Cell & Tissue Engineering
Kei Utsunomiya, Takashi Maruyama, Satoshi Shimizu, Taro Matsumoto, Morito Endo, Hiroki Kobayashi, Koichiro Kano, Masanori Abe, Noboru Fukuda
Summary: Implantation of DFAT cells can improve ANCA glomerulonephritis through immunosuppressive effects, suggesting a potential cell therapy for this disease.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Tomoyasu Otsuki, Noboru Fukuda, Lan Chen, Akiko Tsunemi, Masanori Abe
Summary: In this study, we have demonstrated the role of complement 3 (C3) in inducing epithelial-mesenchymal transition (EMT) phenomenon and renal fibrosis in unilateral ureteral obstruction (UUO) kidney. We investigated the involvement of twist-related protein 1 (TWIST1) in these processes and found that TWIST1 upregulation leads to EMT and renal fibrosis through C3 upregulation. Our findings suggest that targeting TWIST1 with pyrrole-imidazole (PI) polyamides could be a potential therapy for renal fibrosis.
Article
Biochemistry & Molecular Biology
Takuya Hidaka, Kaori Hashiya, Toshikazu Bando, Ganesh N. Pandian, Hiroshi Sugiyama
Summary: This study constructed a class of compounds that can selectively alkylate mutant mitochondrial DNA, with the potential to treat mitochondrial diseases. These compounds have programmability and can be used to target pathogenic mutations associated with mitochondrial diseases in future studies.
CELL CHEMICAL BIOLOGY
(2022)