Article
Hematology
Gregoire Detriche, Nicolas Gendron, Aurelien Philippe, Maxime Gruest, Paul Billoir, Elisa Rossi, Coralie L. Guerin, Anna Lokajczyk, Severine Brabant, Dominique Prie, Tristan Mirault, David M. Smadja
Summary: Gonadotropins increase ECFC-related angiogenesis and may be involved in thrombin generation in cardiovascular disease. Gonadotropins may act as biomarkers; moreover, we hypothesize that gonadotropin-blocking strategies may be a novel interesting therapeutic approach in atherosclerotic cardiovascular disease.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Adeline Blandinieres, Anna M. Randi, Koralia E. Paschalaki, Coralie L. Guerin, Juan M. Melero-Martin, David M. Smadja
Summary: The study aims to provide expert consensus guidance on the isolation and culture of endothelial colony-forming cells (ECFCs). A survey was conducted among 21 experts from 10 different countries to attain a consensus on ECFC isolation and culture. The results indicate the need for standardization and validation of ECFC isolation and culture procedures for clinical laboratory practice.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Pharmacology & Pharmacy
Atanu Sen, Himani Thakkar, Vinnyfred Vincent, Sandeep Rai, Archna Singh, Sujata Mohanty, Ambuj Roy, Lakshmy Ramakrishnan
Summary: This study investigated the relationship between biopterins levels of endothelial colony forming cells (ECFCs) and endothelial progenitor cells (EPCs) in circulation, as well as the functional potential of ECFCs. The results suggest that biopterins play an important role in maintaining the functional integrity of EPCs and ECFCs in circulation.
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stephanie Simoncini, Simon Toupance, Carlos Labat, Sylvie Gautier, Chloe Dumoulin, Laurent Arnaud, Maria G. Stathopoulou, Sophie Visvikis-Siest, Pascal M. Rossi, Athanase Benetos, Francoise Dignat-George, Florence Sabatier
Summary: This study investigates the relationship between atherosclerotic cardiovascular disease (ASCVD), inflammation markers, and circulating endothelial progenitor cells. The findings suggest that ASCVD impairs the colony forming capacity and angiogenic properties of endothelial colony forming cells (ECFC) in a severity-dependent manner. Additionally, alteration of ECFC is associated with increased senescent phenotype and IL-6 levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ana Paula Toledo Del Rio, Jessica O. Frade-Guanaes, Stephanie Ospina-Prieto, Bruno K. L. Duarte, Manoel Barros Bertolo, Margareth C. Ozelo, Zoraida Sachetto
Summary: This study found that ECFCs from patients with PR3-positive GPA in remission demonstrated early losses of tube formation and reduced migration capacity, suggesting impairment of endothelial function.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Qingsong Hu, Xiaobian Dong, Kun Zhang, Huangfeng Song, Cuizhi Li, Tao Zhang, Jianyi Feng, Xiao Ke, Hairui Li, Yangxin Chen, Ruqiong Nie, Xiaoming Chen, Youbin Liu
Summary: This study investigated the effects of prehypertension and shear stress on the reendothelialization potential of early EPCs in humans and explored the potential mechanisms. The study found that early EPCs from prehypertensive patients exhibited reduced migration and adhesion, as well as significantly impaired in vivo reendothelialization capacity. Shear stress pretreatment could significantly promote the in vivo reendothelialization capacity of EPCs. Additionally, CXCR4 receptor plays a crucial role in modulating the reendothelialization potential of early EPCs through regulating JAK-2 signaling.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Klara Komici, Angelica Perna, Aldo Rocca, Leonardo Bencivenga, Giuseppe Rengo, Germano Guerra
Summary: Rheumatoid Arthritis is a chronic autoimmune inflammatory disease that can lead to endothelial damage and dysfunction. Endothelial Progenitor Cells play a crucial role in vascular homeostasis and repair, but there are conflicting results regarding their number and function in RA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Simon Toupance, Stephanie Simonici, Carlos Labat, Chloe Dumoulin, Tsung-Po Lai, Cecile Lakomy, Veronique Regnault, Patrick Lacolley, Francoise Dignat George, Florence Sabatier, Abraham Aviv, Athanase Benetos
Summary: The study found that individuals with longer telomeres have more self-renewing endothelial colony-forming cells. White blood cell telomere length can serve as a surrogate marker for endothelial repair capacity in clinical and laboratory practice, with potential applications.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Medicine, General & Internal
Nadia Meyer, Lars Brodowski, Katja Richter, Constantin S. von Kaisenberg, Bianca Schroeder-Heurich, Frauke von Versen-Hoeynck
Summary: Pravastatin enhances the functional capacities of ECFCs and promotes angiogenic signaling and protein expression, improving endothelial health. These effects may offer a promising option for ameliorating endothelial dysfunction and understanding the pleiotropic function of statins.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Paolo Armanetti, Anastasia Chilla, Francesca Margheri, Alessio Biagioni, Luca Menichetti, Giancarlo Margheri, Fulvio Ratto, Sonia Centi, Francesca Bianchini, Mirko Severi, Rita Traversi, Daniele Bani, Matteo Lulli, Tommaso Del Rosso, Alessandra Mocali, Elisabetta Rovida, Mario Del Rosso, Gabriella Fibbi, Anna Laurenzana
Summary: This study demonstrated that endothelial colony forming cells can effectively deliver gold nanoparticles to tumors, showing potential in cancer treatment. The AuNP-loaded ECFCs were able to migrate to tumor sites in a mouse model and remain in the tumor mass for an extended period without causing damage to healthy tissue. Additionally, the study showed that the ECFC-loaded AuNPs were efficiently cleared by the liver over time.
Article
Biotechnology & Applied Microbiology
Yaqi Tang, Lu Yin, Shuai Gao, Xiaojing Long, Zhanhui Du, Yingchao Zhou, Shuiyan Zhao, Yue Cao, Silin Pan
Summary: Combining synthetic polymers and biomacromolecules can prevent thrombosis and intimal hyperplasia in small-diameter vascular grafts (SDVGs). In this study, a bi-layered scaffold using electrospinning poly (L)-lactic acid (PLLA) is developed to promote the capture and differentiation of endothelial colony-forming cells (ECFCs) and prevent thrombosis after implantation. The scaffold consists of an outer PLLA scaffold and an inner PLLA biomimetic membrane containing heparin, the peptide Gly-Gly-Gly-Arg-Glu-Asp-Val (GGG-REDV), and vascular endothelial growth factor (VEGF). The modified SDVGs showed improved hemocompatibility and successful capture and differentiation of ECFCs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Paul Billoir, Adeline Blandinieres, Nicolas Gendron, Richard Chocron, Sven Gunther, Aurelien Philippe, Coralie L. Guerin, Dominique Israel-Biet, David M. Smadja
Summary: This study investigated hypercoagulability states in ECFCs from patients with IPF, finding higher thrombin potential in IPF-ECFCs and decreased expression of anticoagulant proteins on ECFCs membrane. This new finding suggests a potential role of ECFC in IPF pathophysiology and thrombotic-related disorders.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Nadia Meyer, Lars Brodowski, Constantin von Kaisenberg, Bianca Schroeder-Heurich, Frauke Von Versen-Hoeynck
Summary: This study investigated the effects of CsA and Tac on endothelial colony-forming cells (ECFCs), finding that these immunosuppressants not only impair vascular repair and angiogenesis, but also trigger an inflammatory cascade and increase cardiovascular risk in transplant patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Hyunsook Lee, Yang-Hoon Huh, Kyu-Tae Kang
Summary: This study investigated the role of mesenchymal stem cells (MSCs) in enhancing the vasculogenic capacity of endothelial colony-forming cells (ECFCs) under high-glucose conditions. The researchers found that the combination of ECFCs and MSCs restored the decreased tube formation ability of ECFCs in high-glucose conditions. In vivo experiments using diabetic mice showed that the combined delivery of ECFCs and MSCs led to the formation of perfused blood vessels in diabetic ischemic regions.
Article
Peripheral Vascular Disease
Xenia Kraus, Michael Pflaum, Stefanie Thoms, Rebecca Jonczyk, Martin Witt, Thomas Scheper, Cornelia Blume
Summary: The study aimed to develop a standardized pre-conditioning protocol under dynamic conditions to improve the formation of a flow resistant monolayer of ECFCs and enhance the antithrombogenicity of endothelial cells. High shear stress of 20 dyn/cm(2) led to significant orientation and elongation of ECFCs, forming a confluent endothelial cell layer.
MICROVASCULAR RESEARCH
(2021)
Article
Sport Sciences
Fawn M. Weihl, Gary P. Van Guilder
Summary: The study found that collegiate female swimmers experienced endothelial dysfunction after a high-volume training load, but showed significant improvement in endothelial vasodilation after a tapered training period. This suggests that adjusting training volume and intensity can positively impact vascular function in elite athletes.
JOURNAL OF STRENGTH AND CONDITIONING RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Kelly A. Stockelman, Anthony R. Bain, Caitlin A. Dow, Kyle J. Diehl, Jared J. Greiner, Brian L. Stauffer, Christopher A. DeSouza
Summary: Insufficient sleep is associated with endothelial dysfunction and increased cardiovascular risk, while regular aerobic exercise can improve endothelial function and reduce cardiovascular risk. In this study, aerobic exercise training was found to enhance endothelium-dependent vasodilation and reduce ET-1-mediated vasoconstriction in adults with habitual insufficient nightly sleep, regardless of changes in sleep duration. Regular aerobic exercise can potentially mitigate the cardiovascular risk associated with insufficient sleep-related endothelial dysfunction.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Physiology
Matthew C. Babcock, Lyndsey E. DuBose, Teresa L. Witten, Ashley Brubaker, Brian L. Stauffer, Kerry L. Hildreth, Kerrie L. Moreau
Summary: Aging is associated with reductions in endothelial function, assessed using brachial artery flow-mediated dilation. However, microvessel endothelial function assessed via finger plethysmography may not accurately reflect these age-related changes in large artery endothelial function. The relationship between peripheral artery tonometry and brachial artery ultrasound in evaluating endothelial function in the context of aging needs further investigation.
JOURNAL OF APPLIED PHYSIOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
Gary P. Van Guilder, Claudia C. Preston, Thayne A. Munce, Randolph S. Faustino
Summary: Cardiovascular adaptation is essential for athletic training, and circulating biomarkers play a crucial role in monitoring cardiovascular function. Advances in technology have made novel biomarkers like circulating cell free DNA and RNA attractive tools for this purpose, offering unprecedented precision and resolution in understanding physiological remodeling during exercise-induced stimulation.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Review
Peripheral Vascular Disease
George A. Kelley, Kristi S. Kelley, Brian L. Stauffer
Summary: Isometric exercise (IE) has been shown to reduce resting systolic blood pressure (SBP) and diastolic blood pressure (DBP) in adults, but the results of the current study suggest that random variability, rather than true inter-individual response differences, account for any potential differences in the effects of IE on changes in resting SBP and DBP in adults. Further investigation into potential moderators and mediators, including potential genetic interactions associated with IE, may not be warranted.
Article
Peripheral Vascular Disease
Kelly A. Stockelman, Anthony R. Bain, Anabel Goulding, Noah M. DeSouza, Clay Rahaman, Lukas Maly, Jared J. Greiner, Brian L. Stauffer, Christopher A. DeSouza
Summary: Insufficient nightly sleep is associated with reduced nitric oxide-mediated endothelium-dependent vasodilation and endothelial tPA release in hypertensive adults, likely due to increased oxidative stress.
Article
Endocrinology & Metabolism
Matthew C. Babcock, Lyndsey E. DuBose, Teresa L. Witten, Brian L. Stauffer, Kerry L. Hildreth, Robert S. Schwartz, Wendy M. Kohrt, Kerrie L. Moreau
Summary: Low testosterone in middle-aged/older men is associated with age-associated endothelial dysfunction, potentially due to increased oxidative stress and inflammation.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Environmental Sciences
Riley P. Huffman, Gary P. Van Guilder
Summary: This study found that acetaminophen does not improve running economy or 3 km time trial performance in collegiate distance runners.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Physiology
Matthew C. Babcock, Lyndsey E. DuBose, Kerry L. Hildreth, Brian L. Stauffer, William K. Cornwell, Wendy M. Kohrt, Kerrie L. Moreau
Summary: Low testosterone levels are associated with greater age-associated reductions in cardiovagal baroreflex sensitivity (cBRS) in middle-aged/older men, possibly due to increased inflammation.
JOURNAL OF APPLIED PHYSIOLOGY
(2022)
Article
Biology
Genevieve C. Sparagna, Raleigh L. Jonscher, Sydney R. Shuff, Elisabeth K. Phillips, Cortney E. Wilson, Kathleen C. Woulfe, Anastacia M. Garcia, Brian L. Stauffer, Kathryn C. Chatfield
Summary: This study investigates the relationship between mitochondrial cardiolipin composition and metabolic activity in the rat heart and liver, as well as the impact of altering composition on fatty acid utilization in heart cells. The results show that the enrichment of linoleate in cardiac mitochondria is associated with increased beta-oxidation and mitochondrial respiration. This study provides important insights into the regulatory mechanisms of cardiac metabolism by mitochondrial composition.
Article
Cardiac & Cardiovascular Systems
Megan M. Wenner, Laura M. Welti, Caitlin A. Dow, Jared J. Greiner, Brian L. Stauffer, Christopher A. DeSouza
Summary: Aerobic exercise training reduces ET-1-mediated vasoconstriction and improves endothelium-dependent vasodilation in postmenopausal women.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Frehiwet T. Hailu, Anis Karimpour-Fard, Bonnie Neltner, Brian L. Stauffer, Steven Lipshultz, Shelley D. Miyamoto, Carmen C. Sucharov
Summary: This study compares the levels of miRs in the serum and cardiac tissue of pediatric dilated cardiomyopathy patients and explores the association between the two. Functional analysis suggests that the dysregulation of these miRs is associated with altered pathways related to cell growth, differentiation and proliferation, inflammation, mitochondria function, and metabolism.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Environmental Sciences
Aaron H. Gouw, Gary P. Van Guilder, Gillian G. Cullen, Lance C. Dalleck
Summary: The aim of this study was to assess the reliability and validity of the Tyme Wear smart shirt compared to the Parvo Medics TrueOne 2400 in detecting personalized ventilatory thresholds. The results showed that the smart shirt had high reliability but lower validity compared to the gold standard. Despite underestimations, the ventilatory thresholds detected by the smart shirt still provided adequate training loads for most individuals.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Peripheral Vascular Disease
Tiffany E. Trachte, Brian A. Hemenway, Gary P. Van Guilder
Summary: This study aimed to investigate the impact of clustered cardiovascular disease risk factors on the ability of ischemic preconditioning (IPC) to protect microvascular endothelial function against ischemia-reperfusion (I/R) injury in humans. The results showed that individuals with a greater burden of cardiovascular disease risk factors demonstrated a reduced capacity of IPC to protect against I/R injury.
JOURNAL OF HUMAN HYPERTENSION
(2021)
Article
Physiology
Matthew C. Babcock, Lyndsey E. DuBose, Kerry L. Hildreth, Brian L. Stauffer, William K. Cornwell, Wendy M. Kohrt, Kerrie L. Moreau
Summary: The study suggests that low testosterone may contribute to reductions in cardiovagal baroreflex sensitivity in middle-aged/older men, with increased inflammation potentially playing a role in this process.
JOURNAL OF APPLIED PHYSIOLOGY
(2021)