Article
Cell Biology
Selena Y. Lin, Adam Zhang, Jessica Lian, Jeremy Wang, Ting-Tsung Chang, Yih-Jyh Lin, Wei Song, Ying-Hsiu Su
Summary: The study identified 396 recurrently targeted genes (RTGs) in HBV-associated HCC, with 23 of them associated with carcinogenesis. Among the top three RTGs, TERT, MLL4/KMT2B, and PLEKHG4B were identified as most frequently targeted. Comprehensive analysis of these genes provides insights for potential drug development and disease management in HCC.
Article
Genetics & Heredity
Wenhui Wang, Yan Chen, Liang Wu, Yi Zhang, Seungyeul Yoo, Quan Chen, Shiping Liu, Yong Hou, Xiao-ping Chen, Qian Chen, Jun Zhu
Summary: Our study revealed high heterogeneity of HCC tumor cells in terms of both HBV integrations and CNVs. Two HBV integration hotspots were found across cells, and cells from multiple tumor sites shared some HBV integration and CNV patterns.
BMC MEDICAL GENOMICS
(2022)
Article
Oncology
Wei-Feng Hong, De-Xiang Zhu, Yan-Jie Chen, Xi-Zhong Shen, Yue-Hong Cui, Shi-Suo Du, Tian-Shu Liu, Li Liang
Summary: This study identified differential expression of Coiled-Coil Domain-Containing (CCDC) proteins in colorectal cancer (CRC) and their association with patient survival. High expression of CCDC154 was found to be associated with poor survival and correlated with low infiltration of CD8+ T cells and high infiltration of neutrophils, suggesting its role in promoting tumor growth and metastasis. CCDC154 was also found to interact with Minichromosome Maintenance Complex Component 2 (MCM2) protein and promote malignant phenotype via MCM2.
Article
Microbiology
Maria Bousali, Timokratis Karamitros
Summary: This study investigated the preferential integration of hepatitis B virus (HBV) into specific genes and evaluated their contribution to hepatocellular carcinoma (HCC) development. The results suggested that 15 genes are frequently affected by HBV integrations and significantly associated with HBV-HCC.
Article
Nanoscience & Nanotechnology
Jie Zhao, Li Li, Liying Guo, Rui Wang, Yan Zhao, Wei Li, Yupei Liu, Yanhong Ma, Jianwei Jia
Summary: This study introduced nano-gold PCR for detecting TERT methylation and found that TERT methylation is associated with poor prognosis in hepatocellular carcinoma patients. Nano-gold PCR showed higher sensitivity in detecting TERT methylation compared to conventional PCR. Moreover, as chronic hepatitis B progresses, TERT methylation increases, indicating a worsening prognosis in HCC patients.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2021)
Article
Virology
Pranav P. Mathkar, Xun Chen, Arvis Sulovari, Dawei Li
Summary: Hepatocellular carcinoma (HCC) is a major cause of cancer-related mortality, with about half of cases associated with hepatitis B virus (HBV) infections. Accurate identification of HBV integration sites is crucial for understanding the landscape of HCC genome. Through pathway enrichment analysis, it was found that HBV integrations were linked to various metabolism pathways. The VIcaller method showed potential in identifying novel clonal integrations in cancer-related genes.
Article
Biotechnology & Applied Microbiology
Weiyang Li, Wei Wei, Fei Hou, Hanshi Xu, Xiaofang Cui
Summary: This study used high-throughput long-read sequencing combined with bioinformatics to study the complete mode of HBV integration in HCC cells. The results revealed that short insertion segments accounted for 50% of HBV insertion events, with different lengths of HBV insertion in breakpoints in different regions. Additionally, the occurrence of HBV integration events was associated with more frequent structural variations.
Article
Pathology
Ye Zheng, Mingzhu Xu, Dong Zeng, Haitao Tong, Yuhan Shi, Yanling Feng, Xiaonan Zhang
Summary: This study revealed the differential expression and localization of HBsAg and HBV DNA in tumor tissue and surrounding tissue of HBV-related HCC patients. These findings provide insight into the complex mechanisms of HBV-induced tumorigenesis.
DIAGNOSTIC PATHOLOGY
(2022)
Article
Genetics & Heredity
Hongyuan Cui, Weiqi Rong, Jie Ma, Qing Zhu, Boyue Jiang, Lili Zhang, Chang Li, Zhongling Zhuo, Meng Chen
Summary: DNA methylation on N6-adenine (6mA) has been identified as a potential epigenetic marker in prokaryotes and eukaryotes. This study used Nanopore sequencing to globally profile 6mA sites in the genome of hepatocellular carcinoma, revealing widespread distribution throughout the human genome. The 6mA sites were found to be associated with metabolic processes and gene transcription, and may serve as potential biomarkers for cancer diagnosis and treatment.
Article
Fisheries
Huiming Sun, Wen Huang, Zhizhan Luo, Chuntian Zheng, Guoxia Wang, Peng Luo, Shengpeng Wang, Jinshang Liu, Huo Li, Hu Shu
Summary: The Lv-MCM2 gene plays a crucial role in DNA replication and stress response in Pacific white shrimp, particularly under low-temperature conditions. Single-nucleotide polymorphisms (SNPs) within the Lv-MCM2 gene can be used as molecular markers for breeding cold-tolerant shrimp seeds in the future.
AQUACULTURE REPORTS
(2021)
Review
Microbiology
Maria Bousali, George Papatheodoridis, Dimitrios Paraskevis, Timokratis Karamitros
Summary: HBV is an Old World virus with a high mutation rate, originating in Africa alongside Homo sapiens. It can lead to chronic infection by integrating its genome into host DNA, affecting various genes including those related to cell proliferation and oncogenes.
Article
Endocrinology & Metabolism
Jiangyan Zhou, Faying Liu, Lifeng Tian, Ming Yang, Jun Tan, Xianxian Liu, Peishuang Li, Jia Chen, Ge Chen, Lixian Xu, Lisha Peng, Qiongfang Wu, Yang Zou
Summary: This study investigated whether mutations in the minichromosome maintenance complex component (MCM) family genes were present in Chinese patients with polycystic ovary syndrome (PCOS). Through PCR and Sanger sequencing, genomic DNA was obtained from 365 PCOS patients for analysis. The results identified 29 missense or nonsense mutations/rare variants in the MCM genes, suggesting a high frequency of potential pathogenic mutations in Chinese women with PCOS.
GYNECOLOGICAL ENDOCRINOLOGY
(2023)
Article
Medicine, Research & Experimental
Yu Lei, Shuhui Wang, Jingmei Liu, Wei Yan, Ping Han, Dean Tian
Summary: Systematic analysis revealed that overexpression of MCM in HCC is significantly associated with poor prognosis, with main function enriched in DNA replication. MCM is also linked to cancer pathways and drug sensitivity in HCC.
Article
Gastroenterology & Hepatology
Grace Lai-Hung Wong, Vicki Wing-Ki Hui, Terry Cheuk-Fung Yip, Lilian Yan Liang, Xinrong Zhang, Yee-Kit Tse, Jimmy Che-To Lai, Henry Lik-Yuen Chan, Vincent Wai-Sun Wong
Summary: Universal HBV vaccination markedly reduces the prevalence of chronic HBV infection and may contribute to the decreased incidences of HCC and hepatic events.
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Oncology
Sheng-Han Wang, Shiou-Hwei Yeh, Pei-Jer Chen
Summary: HBV infection is a major risk factor for HCC globally, with chronic hepatitis B generating an inflammatory microenvironment that leads to genetic mutations in hepatocytes predisposing them to cancer. Male dominance in HBV-related HCC highlights the interplay between sex hormones and viral factors influencing carcinogenesis.