Article
Public, Environmental & Occupational Health
A. Rahal, A. Andreo, F. Le Gallou, C. Bourigault, C. Bouchand, C. Ferriot, S. Corvec, A. Guillouzouic, C. Gras-Leguen, E. Launay, C. Flamant, D. Lepelletier
Summary: The report discusses the investigation of an Enterobacter cloacae complex outbreak in a neonatal intensive care unit from November 2020 to February 2021. Pulsed-field gel electrophoresis revealed that five out of eight cases were infected with a clonal strain, potentially due to the shared breast pumps among mothers in the unit. Copyright 2021 The Healthcare Infection Society. Published by Elsevier Ltd.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Immunology
Yang Ji, Peihong Wang, Tingting Xu, Yanzi Zhou, Rongchang Chen, Huaiqiu Zhu, Kai Zhou
Summary: A study aimed to develop a method to distinguish four epidemiologically prevalent species of Enterobacter cloacae complex (ECC) with clinical significance has successfully achieved a high specificity and sensitivity in species-specific PCR, especially for E. cloacae. The established multiplex PCR provides an accurate, expeditious, and cost-effective way for routine diagnosis and molecular surveillance of ECC strains at species level.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Dennis Nurjadi, Martin Scherrer, Uwe Frank, Nico T. Mutters, Alexandra Heininger, Isabel Spaeth, Vanessa M. Eichel, Jonas Jabs, Katja Probst, Carsten Mueller-Tidow, Juliane Brandt, Klaus Heeg, Sebastien Boutin
Summary: The hospital water environment can serve as a reservoir for multiward outbreaks, leading to acquisitions or transmissions of multidrug-resistant organisms in a hospital setting. Most Gram-negative bacteria have the ability to form biofilms and persist in hospital plumbing systems for extended periods. Eliminating these reservoirs is crucial to preventing further transmission, but proposed decontamination regimens, such as using acetic acid, can only suppress rather than fully eliminate the environmental reservoir. This study demonstrated that colonization with multidrug-resistant organisms can be acquired through showering in showers with contaminated water traps and drains.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biotechnology & Applied Microbiology
Min Liao, Yixin Luo, Na Xu, Xiaomei Xie, Xinjun Gan, Dan Cao
Summary: A heterotrophic nitrifier and aerobic denitrifier, identified as Enterobacter cloacae DK-6, was isolated from chaff bedding of a pig farm. Strain DK-6 showed efficient nitrogen removal abilities under aerobic condition, achieving removal efficiencies of 86.98%, 94.66%, and 100% for ammonium-N, nitrate-N, and nitrite-N, respectively. The study also found that strain DK-6 preferred to utilize nitrite-N when mixed nitrogen sources were present, leading to improved nitrate-N removal efficiency. The results suggested that strain DK-6 has significant potential in treating multiple nitrogen pollutants in wastewater under aerobic condition and lower carbon/nitrogen ratio.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Microbiology
Enrique Hernandez-Alonso, Nadege Bourgeois-Nicolaos, Margaux Lepainteur, Veronique Derouin, Simon Barreault, Adam Waalkes, Luis A. Augusto, Stuti Gera, Orane Gleizes, Pierre Tissieres, Stephen J. Salipante, Daniele de Luca, Florence Doucet-Populaire
Summary: This study investigated the management and control measures during an Enterobacter outbreak in a NICU in France. Using whole-genome sequencing, the clinical and microbiological characteristics were identified, and the neonatal incubators were determined to be the primary source of contamination. By implementing a new sampling methodology and replacing the contaminated incubators, the outbreak was successfully contained.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
William Bennett, Katrin Mende, Wesley R. Campbell, Miriam Beckius, Laveta Stewart, Faraz Shaikh, Azizur Rahman, David R. Tribble, Joseph M. Yabes
Summary: This study evaluated the characteristics, antibiotic utilization, and outcomes of battlefield-related Enterobacter cloacae infections. Patients with E. cloacae infections sustained more blast trauma, required more initial blood products, and had longer durations of antibiotic therapy and hospitalizations compared to patients with non-E. cloacae infections.
Editorial Material
Biochemistry & Molecular Biology
Zhiyong Zong, Yu Feng, Alan McNally
Summary: Enterobacter is a globally important pathogen with resistance to carbapenem and colistin. Further research is needed to understand colistin heteroresistance and the emergence of certain Enterobacter lineages clinically.
TRENDS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Fang Liu, Panpan Jin, Zhilan Sun, Lihui Du, Daoying Wang, Tong Zhao, Michael P. Doyle
Summary: This study showed that carvacrol oil inhibits biofilm formation and EPS production of Enterobacter cloacae by down-regulating multiple genes related to biofilm formation. Additionally, the use of other antimicrobial agents can further reduce the number of bacterial cells in carvacrol-treated biofilms.
Article
Infectious Diseases
Delphine Girlich, Souad Ouzani, Cecile Emeraud, Lauraine Gauthier, Remy A. Bonnin, N. Le Sache, Mostafa Mokhtari, Isabelle Langlois, Christine Begasse, Nicolas Arangia, Sandra Fournier, Nicolas Fortineau, Thierry Naas, Laurent Dortet, L. Gauthier PharmD, N. Fortineau, T. Naas, N. Le Sache, C. Emeraud, Laurent Dortet
Summary: This study analyzed the transmission routes of Enterobacter cloacae complex isolates in a neonatal intensive care unit using whole genome sequencing, revealing the impact of E cloacae on fatal septic shocks in neonates and the role of incubators as long-term reservoirs and sources of cross contamination.
Article
Environmental Sciences
Alireza Mohammadzadeh, Mustafa M. Kadhim, Talib Zeedan Taban, Omirserik Baigenzhenov, Andrei Ivanets, Basant Lal, Naveen Kumar, Ahmad Hosseini-Bandegharaei
Summary: In this study, the adsorption properties of Enterobacter cloacae towards uranium were investigated. The results showed that Enterobacter cloacae has a high affinity for fast and selective biosorption of uranium ions. Additionally, a composite material of Enterobacter cloacae/carbon nanotube was synthesized and utilized for preconcentration and determination of uranium, demonstrating low detection limit, high precision, and good accuracy.
Article
Biotechnology & Applied Microbiology
Hsiang-Yen Su, Hua-Ying Li, Cai-Yun Xie, Qiang Fei, Ke-Ke Cheng
Summary: This study engineered E. cloacae to enhance the co-production of succinic acid and acetoin through metabolic flux redirection. By optimizing the culture conditions, the production of acetoin and succinic acid was further increased.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Microbiology
Chen Wang, Mengyun Yin, Xuefei Zhang, Qinglan Guo, Minggui Wang
Summary: A new quinolone resistance gene qnrE3 was identified, providing decreased susceptibility to fluoroquinolones like qnrE1 and qnrE2. Phylogenetic analysis revealed two major clusters, with qnrE3 and qnrE(Eas2) alleles possibly captured and mobilized between different bacterial strains. The study also suggested E. mori as the probable source of qnrE1 to qnrE3 alleles, while E. asburiae serves as the reservoir for qnrE4.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Environmental Sciences
Meiyu Jin, Lianwen Zheng, Yunfei Wei, Jiaqi Cheng, Di Zhang, Siru Yan, Haiyan Qin, Qi Wang, Xinxin Ci, Haihua Feng
Summary: This study found that E. cloacae can promote the development of nonalcoholic fatty liver disease (NAFLD) and atherosclerosis by inducing inflammation and lipid accumulation. These findings are important for studying the pathogenesis and drug screening of these diseases.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Environmental Sciences
Katja Probst, Sebastien Boutin, Isabel Spaeth, Martin Scherrer, Nicole Henny, Delal Sahin, Alexandra Heininger, Klaus Heeg, Dennis Nurjadi
Summary: The study utilized qPCR to detect carbapenemase-producing bacteria during outbreaks, showing a higher sensitivity compared to culture methods. Environmental screening identified reservoirs of carbapenemase genes, indicating potential sources for new outbreaks. Rapid identification of colonized patients and screening of hospital environment are essential for early patient treatment and infection control.
ENVIRONMENTAL RESEARCH
(2022)
Article
Microbiology
Zhi Tao, Di Geng, Jiayue Tao, Jing Wang, Siqi Liu, Qiaoxia Wang, Feng Xu, Shengyuan Xiao, Rufeng Wang
Summary: This study constructed a combination of allicin and Enterobacter cloacae bacteriophage, which effectively lowered the dosage of allicin and overcame bacterial drug-resistance. Allicin interacts with bacterial DNA and disrupts it by cleaving phosphate diester bonds, while the bacteriophage destroys the cell wall and membrane of bacteria. The synergistic effect of this combination improves antibacterial efficiency and avoids drug-resistance.
MICROBIOLOGY SPECTRUM
(2023)
Article
Clinical Neurology
Rodolfo Furlan Damiano, Deusdedit Brandao Neto, Joao Vitor Ribeiro Oliveira, Jonatas Magalhaes Santos, Julia Vallin Rodrigues Alves, Bruno F. Guedes, Ricardo Nitrini, Adriana Ladeira de Araujo, Melaine Oliveira, Andre R. Brunoni, Richard Louis Voegels, Ricardo Ferreira Bento, Geraldo Busatto, Euripedes Constantino Miguel, Orestes V. Forlenza, Fabio de Rezende Pinna
Summary: Preliminary methodologically limited studies suggest an association between chemosensory impairments and neuropsychiatric symptoms in post-COVID-19 patients. This study aims to evaluate the relationship between chemosensory dysfunction and neuropsychiatric impairments in a well-characterized sample of post-COVID-19 patients. The findings indicate a positive association between chemosensory abnormalities and cognitive dysfunction in these patients.
EUROPEAN ARCHIVES OF PSYCHIATRY AND CLINICAL NEUROSCIENCE
(2023)
Article
Public, Environmental & Occupational Health
Andres Mello Lopez, Igor Carmo Borges, Alessandra Luna-Muschi, Carlos Henrique Mesquita Peres, Paolo Gripp Carreno, Arthur Magalhaes de Oliveira, Humberto Bertola Siqueira de Almeida, Vivian Helena de Castro Marques, Felipe Corchs, Anna Sara Levin, Silvia Figueiredo Costa, Ana Marli Christovam Sartori
Summary: A survey of healthcare workers in Sao Paulo, Brazil found that younger age, previous COVID-19 infection, and burnout symptoms were associated with reduced adherence to protective measures following vaccination.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Ana Paula M. Porto, Igor C. Borges, Lewis Buss, Anna Machado, Bil R. Bassetti, Brunno Cocentino, Camila S. Bicalho, Claudia Carrilho, Cristhieni Rodrigues, Eudes A. S. Neto, Evelyne S. Girao, Filipe Piastrelli, Giovanna Sapienza, Glaucia Varkulja, Karin Kolbe, Luciana Passos, Patricia Esteves, Pollyana Gitirana, Regia D. F. Feijo, Rosane L. Coutinho, Thais Guimaraes, Tiago L. L. Ferraz, Anna S. Levin, Silvia F. Costa
Summary: There was an increase in CLABSI incidence in Brazilian ICUs during the first months of the COVID-19 pandemic. Additionally, there was an increase in the proportion of CLABSI caused by E. faecalis and Candida spp during this period.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Immunology
Adriana Coracini Tonacio, Ana Paula Marchi, Maria Luiza Bazzo, Gabriela Takeshigue Lemos, Jose Victor Bortolotto Bampi, Evelyn Patricia Sanchez Espinoza, Edson Luiz Tarsia Duarte, Roberta Cristina Ruedas Martins, Ana Paula Ramalho da Costa-Lourenco, Vitor Falcao de Oliveira, Marina Farrel Cortes, Sania Alves dos Santos, Lauro Vieira Perdigao Neto, Raquel Regina Bonelli, Maria Rita Elmore, Flavia Rossi, Gwenda Hughes, Silvia Figueiredo Costa
Summary: This study presents a rare case of gonococcal arthritis in a patient with Systemic Lupus Erythematosus (SLE). Whole genome sequencing (WGS) and molecular analysis were performed on the etiologic agent, which was found to be resistant to ciprofloxacin and tetracycline. The isolate had genetic features related to beta-lactam, tetracycline, and quinolone resistance, and 71 virulence genes were identified.
MICROBES AND INFECTION
(2023)
Article
Public, Environmental & Occupational Health
Elisa Teixeira Mendes, Danilo Glauco Pereira Villagelin Neto, Giulia Magalhaes Ferreira, Ian Nunes Valenca, Maria Patelli Juliani Souza Lima, Maria Fernanda Marciano Barros de Freitas, Maria Rita Donalisio, Marcio Cristiano Melo, Carolina Lazari, Jacqueline Goes, Ingra Morales, Ana Carolina Gomes Jardim, Pamela Andrade dos Santos, Lucas Augusto Moyses Franco, Ester Cerdeiro Sabino, Silvia Figueiredo Costa
Summary: This study evaluated the prevalence of RT-PCR positivity in asymptomatic healthcare workers (HCW) and its impact on absenteeism. A significant decrease in absenteeism was detected 3-4 weeks after the intervention. The testing and leave policy for asymptomatic professionals contributed to the control strategy for COVID-19 transmission in the hospital environment.
AMERICAN JOURNAL OF INFECTION CONTROL
(2023)
Article
Multidisciplinary Sciences
Ana Rubia Guedes, Maura S. S. Oliveira, Bruno M. M. Tavares, Alessandra Luna-Muschi, Carolina dos Santos Lazari, Amanda C. C. Montal, Elizabeth de Faria, Fernando Liebhart Maia, Antonio dos Santos Barboza, Mariana Deckers Leme, Francis M. M. Tomazini, Silvia Figueiredo Costa, Anna S. S. Levin
Summary: In this study, the rate of reinfection by SARS-CoV-2 over a two-year period of the COVID-19 pandemic was estimated in a large cohort of healthcare workers. It was found that approximately 5% (284 cases) of symptomatic cases experienced reinfection, with the majority occurring during the Omicron variant period. All cases of reinfection were mild.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Ana Paula Marchi, Marina Farrel Cortes, Saidy Vasconez Noguera, Flavia Rossi, Anna Sara Levin, Silvia Figueiredo Costa, Lauro Vieira Perdigao Neto
Summary: The aim of this study was to evaluate the susceptibility of 32 gram-negative and 6 gram-positive isolates to chlorhexidine gluconate (CHG) in both planktonic cells and biofilm. The study found that gram-negative isolates had higher minimal concentrations than gram-positive isolates, and bacteria in biofilms were more tolerant than planktonic ones. There was no statistically significant correlation between minimal bactericidal concentration or minimal biofilm elimination concentration and biofilm formation. The occurrence of the Eagle effect was observed in 60.5% of the isolates, resulting in minimal concentration of CHG as high as 16,384 mu g/mL. Attention should be given to this phenomenon to avoid false susceptibility results.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Review
Virology
Bruno Azevedo Randi, Hermes Ryoiti Higashino, Vinicius Ponzio da Silva, Erick Menezes Xavier, Vanderson Rocha, Silvia Figueiredo Costa
Summary: This study analyzed the clinical characteristics and outcomes of COVID-19 in adult hematopoietic stem-cell transplantation (HSCT) recipients through a systematic review and meta-analysis. The findings showed that symptomatic infection of SARS-CoV-2 was common in HSCT patients and the mortality rate was higher compared to the general population. It is crucial to implement measures to mitigate the risk of SARS-CoV-2 infection in this population and carefully evaluate HSCT recipients who develop COVID-19.
REVIEWS IN MEDICAL VIROLOGY
(2023)
Article
Immunology
Ronaldo Cesar Borges Gryschek, Marcelo Andreetta Corral, Renata Barnabe Sitta, Maiara Gottardi, Ligia Camera Pierrotti, Silvia Figueiredo Costa, Edson Abdala, Pedro Paulo Chieffi, Fabiana Martins de Paula
Summary: This study aimed to observe S. stercoralis infection in transplant candidates using different techniques, and propose an algorithm for the detection of this infection. Parasitological techniques found that 10% of fecal samples were positive. Anti-Strongyloides antibodies IgG were detected in 19.3% and 20.7% of patients by immunofluorescence assay and enzyme-linked immunosorbent assay, respectively. S. stercoralis DNA was observed in 17.3% of samples by conventional polymerase chain reaction and 32.7% of samples by quantitative polymerase chain reaction (qPCR).
TRANSPLANT INFECTIOUS DISEASE
(2023)
Article
Endocrinology & Metabolism
Lais Isidoro Alves, Adriana Aparecida Bosco, Adriana Aparecida Rosa, Marcia Regina Soares Correia, Sergio Russo Matioli, Maria Elizabeth Rossi da Silva
Summary: This study analyzed the differences between diabetic and non-diabetic COVID-19 inpatients. The results showed that diabetic patients were more likely to have abnormal glucose metabolism, inflammatory response, and organ damage, and had a higher mortality rate. Factors related to blood glucose levels, age, gender, and BMI also influenced the mortality risk in diabetic patients.
DIABETOLOGY & METABOLIC SYNDROME
(2023)
News Item
Health Care Sciences & Services
Anna S. Levin, Silvia F. Costa, Maria Tereza P. Razzolini, Maria Clara Padoveze, Fatima L. S. Nunes
LANCET REGIONAL HEALTH-AMERICAS
(2023)
Article
Infectious Diseases
Luis Arthur Brasil Gadelha Farias, Natalia Nogueira Firmino, Marcos Maciel Sousa, Mateus Lavor Lira, Leonardo Nogueira Meireles, Angela Maria Veras Stolp, Kelma Maria Maia, Silvia Figueiredo Costa, Lauro Vieira Perdigao Neto
Summary: Streptococcus constellatus, a gram-positive coccus member of the Streptococcus anginosus group, can be found in the oral flora and commonly cause abscesses in the gastrointestinal tract, lungs, and heart. While Streptococcus species other than S. pneumoniae are rare causes of brain abscesses, this case report highlights a severe case of extra and intracranial abscesses caused by S. constellatus in an immunocompetent host.
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO
(2023)
Article
Nursing
Caroline Lopes Ciofi-Silva, Luciana Cordeiro, Naila Albertina Oliveira, Giulia Marcelino Mainardi, Anna Sara Levin, Rodrigo Maximiano Antunes de Almeida, Juliana Falasco-Fantinatti, Maria Clara Padoveze
Summary: This study aims to adapt, validate, and assess the reliability of the National Aeronautics and Space Administration - Task Load Index instrument translated into Brazilian Portuguese. Through five steps, including translation, synthesis, back-translation, assessment by an expert committee, and pre-test, the final Portuguese version was found to have reliability and content validity evidence.
REVISTA BRASILEIRA DE ENFERMAGEM
(2023)
