Article
Cell Biology
Selina Kiefer, Julia Huber, Hannah Fuellgraf, Kristin Soerensen, Agnes Csanadi, Maren Nicole Stillger, Martin Werner, Hans-Eckart Schaefer, Peter Bronsert, Konrad Aumann
Summary: This study observed colour changes in tissue marking dyes (TMD) during routine immunohistochemistry, with blue TMD from provider A changing to black when DAB and H2O2 were applied. Other colours and TMD from different providers did not show any changes during immunohistochemistry.
Article
Oncology
Saroj Rajthala, Harsh Dongre, Himalaya Parajuli, Anjie Min, Elisabeth Sivy Nginamau, Arild Kvalheim, Stein Lybak, Dipak Sapkota, Anne Christine Johannessen, Daniela Elena Costea
Summary: This study aimed to establish a robust method for dual visualization of miR and protein by combining chromogen-based in situ hybridization and immunohistochemistry, and apply it to investigate stromal expression of miR204 as a putative prognostic biomarker in oral squamous cell carcinoma. The study found that miR204 stromal expression at the tumor front predicted recurrence-free survival (p = 0.032) and overall survival (p = 0.036), and was further confirmed as an independent prognostic biomarker in OSCC through multivariate Cox regression analysis.
Article
Otorhinolaryngology
Andreas P. Keller, Fabienne Grothe, Norbert Stasche, Bjoern Boysen, Niklas Kahl
Summary: The study tested a new optical microscope filter, H.A.S.I., for microlaryngoscopy, which improved the visualization of vocal cord blood vessels and helped in diagnosis and treatment. It was found to be practical and helpful in microlaryngeal laser surgery, not requiring additional equipment.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2022)
Article
Chemistry, Analytical
Bjorn Paulson, Saeed Bohlooli Darian, Youngkyu Kim, Jeongmin Oh, Marjan Ghasemi, Kwanhee Lee, Jun Ki Kim
Summary: Complex clinical and small-animal research procedures can benefit from dual-site imaging with the proposed endoscopic system, which enables multiple fluorescence microendoscopes to share light sources and optical relays on a single microscope base. The system achieves high channel isolation and submicron and subcellular resolution, as demonstrated by imaging experiments on USAF charts and dyed fiber tissues. Simultaneous liver and kidney imaging was also successfully demonstrated in mice. This multi-channel microendoscopy system provides a potentially low-cost means of simultaneous multiple endoscopic imaging, reducing animal harm and enhancing understanding of temporal connections between biological systems.
Article
Biochemical Research Methods
Thomas Kirchner, Martin Frenz
Summary: This study proposes a method for quantitative measurement of blood oxygen saturation using optoacoustic imaging, which combines multiple illumination sensing with learned spectral decoloring. The results show high estimation accuracy and fewer outliers compared to previous methods, with random forest regressors outperforming neural network approaches. Random forest-based MI-LSD is considered a promising method for accurate quantitative OA oximetry imaging.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Dermatology
Sanja Javor, Giulia Gasparini, Chiara Maria Biatta, Emanuele Cozzani, Francesco Cabiddu, Jean Louis Ravetti, Valerio Gaetano Vellone, Aurora Parodi
Summary: Research has shown that p53 staining index in AKs is significantly correlated with age, location, and dysplasia grade. Additionally, there is a significant correlation between p53 staining index and zonal staining patterns. No significant differences in p53 staining index were observed among different histological types of AKs.
ARCHIVES OF DERMATOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Indra Memdi Khoris, Akhilesh Babu Ganganboina, Tetsuro Suzuki, Enoch Y. Park
Summary: By co-precipitating the chromogen TMB in aqueous solution, collective nanoparticles TMB-NPs were formed and enriched in PLGA nanovesicles using a molecular delivery approach. The developed colorimetric sensor demonstrated high sensitivity in detecting influenza virus and other respiratory viruses, showcasing practical applications for airborne virus detection.
Article
Pathology
Anne-Sophie van der Werf-'t, Diantha Terlouw, Carli M. Tops, Merel S. van Kan, Liselotte P. van Hest, Hans J. P. Gille, Floor A. M. Duijkers, Anja Wagner, Ellis L. Eikenboom, Tom G. W. Letteboer, Mirjam M. de Jong, Sanne W. Bajwa-ten Broeke, Fonnet E. Bleeker, Encarna B. Gomez Garcia, Niels de Wind, J. Tom van Wezel, Hans Morreau, Manon Suerink, Maartje Nielsen
Summary: This study aimed to identify the various causes of the discordant phenotypes in MSH6-associated Lynch syndrome, including abnormal immunohistochemical staining and mutations in the mismatch repair genes. The results suggest that genetic analysis should be considered in colorectal cancer patients with a strong family history.
Article
Cell Biology
Maelle Saliba, Nora Katabi, Snjezana Dogan, Bin Xu, Ronald A. Ghossein
Summary: The study evaluated the diagnostic utility of NRAS Q61R immunohistochemistry in thyroid lesions, demonstrating a high sensitivity and specificity for detecting RAS Q61R mutations, particularly in follicular-patterned neoplasms. When evaluated alongside histological features, NRAS Q61R immunoreactivity can be instrumental in the diagnosis and classification of thyroid nodules.
Article
Chemistry, Analytical
Nazan Altun, Martin F. Hervello, Felipe Lombo, Pelayo Gonazlez
Summary: Currently, the lack of reference methods is hindering the full potential of spectral imaging, despite its ability to provide valuable information. This study proposes a staining-based reference method with digital image treatment for spectral imaging, aiming to predict the presence of biofilms in a fast, efficient, contactless, and non-invasive way.
Article
Biochemistry & Molecular Biology
Charlie Colin-Pierre, Valerie Untereiner, Ganesh D. Sockalingum, Nicolas Berthelemy, Louis Danoux, Vincent Bardey, Solene Mine, Christine Jeanmaire, Laurent Ramont, Stephane Brezillon
Summary: This study utilized infrared spectral imaging (IRSI) to investigate the expression and distribution changes of glypicans in different hair follicle compartments during various phases of the hair growth cycle, providing a new perspective on hair shaft growth. The results demonstrate the capability of IRSI to simultaneously reveal the location of proteins, PGs, GAGs, and sulfated GAGs in hair follicles in a reagent- and label-free manner.
Review
Optics
Longqian Huang, Ruichen Luo, Xu Liu, Xiang Hao
Summary: This article reviews state-of-the-art deep-learning-empowered computational spectral imaging methods, which possess fast reconstruction speed, excellent reconstruction quality, and the potential to reduce system volume significantly.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Biology
Jennifer Eng, Elmar Bucher, Zhi Hu, Ting Zheng, Summer L. Gibbs, Koei Chin, Joe W. Gray
Summary: The researchers developed a Python software called mplexable, which is used for image processing in multiplex imaging, and shared their optimization of signal removal, antibody specificity, background correction, and batch normalization through Jupyter notebooks. This work improves the CyCIF methodology and provides a framework for easily sharing and reproducing multiplexed image analytics.
COMMUNICATIONS BIOLOGY
(2022)
Article
Oncology
Sean P. Kelly, Dipak B. Ramkumar, Zachary S. Peacock, Erik T. Newman, Connor Venrick, Santiago A. Lozano-Calderon, Kevin A. Raskin, Ivan Chebib, Joseph H. Schwab
Summary: This study investigated the expression of SOST in giant cell tumor of bone (GCTB) and its correlation with predictor variables. The results showed that SOST staining was present in the majority of GCTB cases and was associated with tumor aggressiveness, patient symptoms, previous surgery, and age. This novel finding warrants further research on the role of SOST as a prognostic factor and therapeutic target in GCTB.
JOURNAL OF SURGICAL ONCOLOGY
(2022)
Article
Biology
Ilan Bejar, Jacob Rubinstein, Jacob Bejar, Edmond Sabo, Hilla K. Sheffer, Zaher Bahouth, Sarel Halachmi, Zahava Vadasz
Summary: Semaphorin 3A (Sema3A) is overexpressed in patients with urothelial carcinoma (UC), particularly in the mucosal layers of high-grade UC patients. Sema3A may serve as a potential non-invasive marker for urothelial cancer.
