Article
Chemistry, Organic
Janeala J. Morsby, Kirk M. Atkinson, Sai Shradha Reddy Kommidi, Tristan Freel, Hana Janekova, Peter Stacko, Bradley D. Smith
Summary: Two new classes of near-infrared molecular probes were developed that exhibit turn on fluorescence when cleaved by the nitroreductase enzyme. These probes have structures that enhance fluorescence brightness and provide better performance when excited by 808 nm light. One of the probes showed higher activity and less self-aggregation when applied in vivo, making it the preferred choice.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Shravan Sreenivasan, Deepak Sonawat, Anurag S. Rathore
Summary: An established platform for determining the size distribution of therapeutic IgG aggregates utilized image processing algorithms for brightfield and fluorescence microscope images. The algorithm involved conversion to grayscale, thresholding, morphological operations, and denoising steps for fluorescence images of tagged aggregates. The resulting size distribution was compared with experimental techniques, showing potential as an orthogonal methodology for analyzing IgG aggregates.
PHARMACEUTICAL RESEARCH
(2021)
Article
Chemistry, Analytical
Ryosuke Kaneko, Masumi Kawamura, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama
Summary: Chloroacetyl group modification can enhance the retention of fluorescent substrates in cells by forming a covalent bond with intracellular proteins, however, its slow reaction speed may lead to dissociation in the early stage of staining.
ANALYTICAL SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yi Liu, Hong Bao, Mei-Li Zhu, Cheng-Xi Hu, Zhi-Gang Zhou
Summary: In this study, MiLPEAT, an important enzyme involved in phospholipid remodeling, was cloned from an arachidonic acid-rich green microalga. The functional analysis confirmed the role of MiLPEAT in acylating lysophosphatidylethanolamine to phosphatidylethanolamine. The localization of MiLPEAT on the microalgal plasma membrane was determined through immunocytochemical observations. This study lays the foundation for understanding the synthesis of TAG in the microalga.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Multidisciplinary Sciences
Prashant Kumar, Prakash Joshi, Jigmi Basumatary, Partha Pratim Mondal
Summary: The proposed optical flow imaging technique (VFC/iLIFE) adds new capabilities to the existing system and enables real-time monitoring and analysis of sub-cellular organelle organization. It provides 3D volume visualization, organelle-level resolution, and multi-organelle screening. This technique allows for high throughput investigation and instant volume reconstruction with organelle-level resolution of live specimens, providing valuable insights into the progression of diseases.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
Margaret N. Ruesch, Luca Benetti, Eileen Berkay, David J. Cirelli, Neha Frantz, Martin H. Gastens, Wayne P. Kelley, Juliana Kretsinger, Mike Lewis, Shawn Novick, Barbara Rellahan, Laura Pack, Corne J. M. Stroop, Ann Subashi, Ping Yin, Ming Zeng, John Stults
Summary: Commercial specifications for new biotherapeutic products are crucial for ensuring safety and efficacy. Setting specifications based on compendia, regulatory guidance, product knowledge, and manufacturing process capability is essential. Patient-centric specifications are needed to control high-risk attributes while allowing flexibility for a sustainable supply chain.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Plant Sciences
Luise Jaeckel, Arianne Schnabel, Hagen Stellmach, Ulrike Klauss, Susanne Matschi, Gerd Hause, Thomas Vogt
Summary: This study provides experimental evidence on the cellular localization and biosynthesis of piperine and piperamide synthase in specific cells of black pepper fruits. Piperine and piperamide synthase accumulate during early stages of fruit development and decline before maturity, and their co-localization is evidenced by fluorescence microscopy. The increasing levels of piperine during fruit maturation are consistent with the increasing numbers of fluorescent cells within the fruit perisperm.
Article
Environmental Sciences
Francesca Marcovecchio, Cinzia Perrino
Summary: The atmospheric concentration of bioparticles was determined in outdoor and indoor sites using a commercial low-volume sampler and a detection method based on particle collection on polycarbonate filters. Higher concentrations of PBAPs were recorded during the warm period, with differences in shape and mass distribution. Indoor PBAPs concentrations were much higher compared to outdoors, with wide polyhedral-shaped particles identifiable as skin flakes.
Article
Chemistry, Analytical
Anna Ryschich, Yan Dong, Michael Schaefer, Eduard Ryschich, Svetlana Karakhanova
Summary: This study developed and characterized a novel antibody clone, DWH24, which can visualize and quantify dead cells. Unlike conventional markers, DWH24 binding is independent of calcium and protein, making it a versatile tool for cell death analysis.
METHODS AND APPLICATIONS IN FLUORESCENCE
(2023)
Review
Biotechnology & Applied Microbiology
Jingjie Jiang, Guangyu Yang, Fuqiang Ma
Summary: Fluorescence-activated droplet sorting (FADS) is a powerful tool for high-throughput screening of enzymes, metabolites, and antibodies. Fluorescence coupling strategies (FCSs) play a key role in developing new FADS methods by linking analyte properties to fluorescence signals. This review highlights recent advances in FCS for different analyte types and discusses successful FADS applications.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Agriculture, Multidisciplinary
Yong Ho Shin, Magda Ledesma, Sonia Whitman, Joy Tyson, Birgit Zange, Ki Deok Kim, Yong Chull Jeun
Summary: The study found that pyraclostrobin + boscalid and iminoctadinetris were the most effective agrochemicals in suppressing ripe rot caused by Botryosphaeria dothidea on kiwifruit. The use of these agrochemicals inhibited the germination of fungal conidia, leading to decreased fungal growth on the fruit.
PLANT PATHOLOGY JOURNAL
(2021)
Article
Pharmacology & Pharmacy
Rasmus Eliasen, Thomas Lars Andresen, Jannik Bruun Larsen
Summary: Lipid nanoparticles have shown clinical success in vaccines, but better tools are needed to analyze heterogeneities at the single particle level. Here, we present a fluorescence microscopy based assay to detect enzyme mediated dePEGylation of individual liposomes, and investigate the factors affecting this process.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Correction
Biochemical Research Methods
Chen LI, Adele Moatti, Xuying Zhang, H. Troy Ghashghaei, Alon Greenbaum
Summary: This note from the publisher corrects the spelling of the fifth author's name in [Biomed. Opt. Express 12, 5214 (2021)].
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Medicinal
Masato Kiyoshi, Minoru Tada, Hiroko Shibata, Michihiko Aoyama, Akiko Ishii-Watabe
Summary: The impact of silicone oil (SO) on antibodies in pre-filled syringes has been studied, revealing that SO accelerates the increase in sub-visible particles and antibody aggregation, leading to the formation of antibody-SO complexes. Further experiments showed that pre-mixed and agitated Ab + SO have higher Fc gamma Rs activation capability compared to agitated Ab alone.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Biochemical Research Methods
Xinyi Zhu, Liangtao Gu, Rui Li, Liang Chen, Jingying Chen, Ning Zhou, Wuwei Ren
Summary: In this study, a cost-effective and comprehensive toolkit named MiniMounter is proposed, which enhances image quality, reduces time for experimental operations and image evaluation, and accelerates the research process for users and developers within the Miniscope community.
JOURNAL OF BIOPHOTONICS
(2023)
