Article
Biochemistry & Molecular Biology
Mengya Wang, Weihao Song, Chaofan Jin, Kejia Huang, Qianwen Yu, Jie Qi, Quanqi Zhang, Yan He
Summary: Pax3 and Pax7 are crucial for muscle development, with distinct populations of muscle satellite cells in black rockfish marked by SsPax3 and SsPax7. SsPax3a and two SsPax7 genes show increased expression during muscle repair, while SsPax3b remains unchanged, suggesting a more complex muscle stem cell population in teleosts compared to mammals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Jonas M. D. Enander, Gerald E. Loeb, Henrik Jorntell
Summary: By modeling the connectivity patterns between the musculoskeletal system and neural circuits, researchers have found that learning induced by Hebbian adaptation during early development can lead to cooperative connections between interneurons, improving the coordination of muscle activities.
JOURNAL OF NEUROPHYSIOLOGY
(2022)
Article
Cell & Tissue Engineering
Congshan Sun, Suraj Kannan, In Young Choi, HoTae Lim, Hao Zhang, Grace S. Chen, Nancy Zhang, Seong-Hyun Park, Carlo Serra, Shama R. Iyer, Thomas E. Lloyd, Richard M. Lovering, Su Bin Lim, Peter Andersen, Kathryn R. Wagner, Gabsang Lee, Chulan Kwon
Summary: Human pluripotent stem cell (hPSC)-derived myogenic progenitor cell (MPC) transplantation can contribute to muscle fiber regeneration in mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders.
Article
Cell Biology
Kwantae Kim, Jameson Orvis, Alberto Stolfi
Summary: Pax3/7 factors play crucial roles in the development of the nervous system in vertebrates and are also involved in similar functions in the model tunicate Ciona. The study reveals that Pax3/7 is expressed in the neural plate borders and central nervous system after neural tube closure, playing a conserved role in neural tube closure and neuron specification. These results suggest that the key functions of Pax3/7 in neural tube development are evolutionarily ancient, dating back to the common ancestor of vertebrates and tunicates.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Oscar Horwath, Fabian Nordstrom, Ferdinand von Walden, William Apro, Marcus Moberg
Summary: Cumulative evidence supports the hypothesis that hypoxia acts as a regulator of muscle mass. This study examined the effect of hypoxia on signaling pathways and gene expression in human skeletal muscle after resistance exercise. The results showed that hypoxia attenuated ribosome signaling but did not affect satellite cell pool expansion and myogenic gene expression.
Article
Cell Biology
Bingqing Huo, Yang Yang, Manhui Li, Jun Wan, Wei Zhang, Bo Yu, Xiaofan Chen
Summary: Pax3 inhibits cell viability and proliferation, affects cell cycle and neurite outgrowth in Neuro-2a cells; Pax7 overexpression has no significant impact on cell viability and proliferation; Pax3 up-regulated genes are involved in cell cycle machinery, potentially revealing the mechanism of Neuro-2a cell proliferation.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Fabian Montecino, Natalia Gonzalez, Natasha Blanco, Manuel J. Ramirez, Adrian Gonzalez-Martin, Alejandra R. Alvarez, Hugo Olguin
Summary: The study reveals the role of c-Abl in regulating myogenic progression in satellite cells (SCs) by controlling Pax7 function and expression, affecting the activity and muscle regeneration of SCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Christine M. Heske, Yueh-Yun Chi, Rajkumar Venkatramani, Minjie Li, Michael A. Arnold, Roshni Dasgupta, Susan M. Hiniker, Douglas S. Hawkins, Leo Mascarenhas
Summary: In patients with localized, FOXO1 fusion-positive RMS, clinical factors such as age at diagnosis and tumor size can impact survival outcomes, with older age and larger tumors being adverse prognostic factors for EFS. Patients with both older age and large tumor size experience significantly inferior outcomes.
Article
Agriculture, Dairy & Animal Science
Jae Ho Han, Si Won Jang, Ye Rim Kim, Hoon Jang, Kwan Seob Shim, Hyun Woo Choi
Summary: The objective of this study was to investigate the appropriate concentration of fibronectin coated dishes for proliferation and maintenance of porcine satellite cells at long-term culture. The results showed that 5μg/mL fibronectin effectively maintains the growth and expression of Pax7 in porcine satellite cells.
Article
Oncology
Massimo Ganassi, Francesco Muntoni, Peter S. Zammit
Summary: This study explores the effects of pathogenic mutations on satellite cell function in muscle diseases. The findings suggest that these mutations can directly or indirectly affect satellite cell function, leading to muscle pathology. The study introduces a classification of satellite cell-opathies, which helps in diagnosis, prognosis, and treatment development.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Genetics & Heredity
Line J. Manceau, Julien E. Richard Albert, Pier-Luigi J. Lollini, Maxim V. C. E. Greenberg, Pascale J. Gilardi-Hebenstreit, Vanessa E. Ribes
Summary: The chimeric proteins PAX3-FOXO1 and PAX7-FOXO1, derived from chromosomal translocations, exhibit distinct genomic occupancy and transcriptional activity, leading to different pathological manifestations in alveolar rhabdomyosarcoma.
Article
Cell Biology
Elke Albrecht, Yaolu Zhao, Quentin L. L. Sciascia, Cornelia C. C. Metges, Steffen Maak
Summary: One way to improve the growth of low-birth-weight piglets is by stimulating muscle development through optimized amino acid supply. In this study, the effects of glutamine (Gln) supplementation on muscle tissue of low-birth-weight and normal-birth-weight piglets were investigated. The results showed that satellite cells and macrophages were the main types of proliferating cells in skeletal muscle early after birth, and Gln supplementation somewhat stimulated satellite cells. There were differences between the two muscles, but no influence of birth weight on the results was observed. Therefore, Gln supplements may not be considered an effective treatment for improving muscle growth in low-birth-weight piglets.
Article
Cell Biology
Philip Barrett, Tom J. Quick, Vivek Mudera, Darren J. Player
Summary: This study aimed to generate and characterize intrafusal bag and chain myotubes from differentiated C2C12 myoblasts using the addition of Neuregulin 1 protein. The results showed that Nrg-1 treatment increased the number of intrafusal bag myotubes and enhanced the expression of the specific transcription factor Egr3. Additionally, Nrg-1 treatment had a proliferative effect during the serum starvation-initiated differentiation phase. Although there was no clear evidence for specific intrafusal development, Nrg-1 treated myotubes exhibited some characteristics of intrafusal fibres, such as increased satellite cell numbers and smaller myonuclear domains.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Lisa S. Chow, Darko Bosnakovski, Douglas G. Mashek, Michael Kyba, Rita C. R. Perlingeiro, Alessandro Magli
Summary: This study investigated the epigenetic changes occurring during the transition from activated satellite cells to myoblasts by performing chromatin accessibility profiling. The analysis identified a compendium of putative regulatory elements defining human activated satellite cells and myoblasts, some of which are shared by both murine and human satellite cells. Integration of transcriptional and epigenetic data revealed potential targets for regulators of metabolic gene expression, indicating an important starting point for understanding the molecular mechanisms underlying human satellite cell muscle regenerative potential.
STEM CELL RESEARCH
(2021)
Review
Cell Biology
Chintan K. Kikani
Summary: Tissue regeneration is a complex process that involves the coordination of cellular responses and stem cell fate. PASK, a sensory protein, plays a critical role in balancing self-renewal and differentiation programs during mammalian tissue regeneration.