期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 57, 期 4, 页码 373-382出版社
HISTOCHEMICAL SOC INC
DOI: 10.1369/jhc.2008.952895
关键词
thymus; mesenchymal cells; lymphotoxin; beta receptor-deficient mice; Plt/plt mice; adult
类别
Thymic mesenchymal cells are known to be important for the development of the early fetal thymus into a functionally mature organ supporting T cell differentiation. We examined the expression of mesenchymal markers: pan-mesenchymal marker ER-TR7, desmin, alpha-smooth muscle actin (alpha-SMA), and alpha- and beta-chain of platelet-derived growth factor receptor (PDGFR alpha, PDGFR beta) in thymi of normal adult mice. Desmin and ER-TR7 revealed specific staining in the capsule, septa, and perivascular cells. Most perivascular cells highly expressed PDGFR beta at the same levels as desmin. Low expression of PDGFR alpha was detected in the capsule, intralobular septa, and some perivascular cells of normal adult thymi. alpha-SMA, used to identify vascular smooth muscle cells, was detectable on arterioles and some large venules but not on capillaries. Thus, desmin, PDGFR alpha, and PDGFR beta were localized in the capsule, septa, and perivascular cells in thymus of adult mouse, although there were differences in the expression level among these markers. On the other hand, the expression of mesenchymal markers was detectable in the region of the thymic medullary epithelium of lymphotoxin beta receptor-deficient mice and plt/plt mice, indicating that mesenchymal cells were abnormally localized in the region. These results suggest that disorganization of the medullary epithelium may be accompanied by aberrant distribution of mesenchyme in adult mouse thymus. (J Histochem Cytochem 57:373-382, 2009)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据