期刊
JOURNAL OF HEREDITY
卷 104, 期 6, 页码 798-806出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jhered/est061
关键词
genomic compartmentalization; heterochromatin; monitor lizard; Platynota; satellite DNA
资金
- National Research Council of Thailand [2556096001002, 2556096003004]
- Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Kasetsart University, Thailand (CASTNAR, NRU-KU, Thailand)
- Fellowship of Capacity Building for Kasetsart University on Internationalization
- Ministry of Education, Culture, Sports, Science and Technology, Japan [22370081]
- Grants-in-Aid for Scientific Research [22370081] Funding Source: KAKEN
Two novel repetitive DNA sequences, VSAREP1 and VSAREP2, were isolated from the water monitor lizard (Varanus salvator macromaculatus, Platynota) and characterized using molecular cytogenetics. The respective lengths and guanine-cytosine (GC) contents of the sequences were 190bp and 57.5% for VSAREP1 and 185bp and 59.7% for VSAREP2, and both elements were tandemly arrayed as satellite DNA in the genome. VSAREP1 and VSAREP2 were each located at the C-positive heterochromatin in the pericentromeric region of chromosome 2q, the centromeric region of chromosome 5, and 3 pairs of microchromosomes. This suggests that genomic compartmentalization between macro- and microchromosomes might not have occurred in the centromeric repetitive sequences of V. salvator macromaculatus. These 2 sequences did only hybridize to genomic DNA of V. salvator macromaculatus, but no signal was observed even for other squamate reptiles, including Varanus exanthematicus, which is a closely related species of V. salvator macromaculatus. These results suggest that these sequences were differentiated rapidly or were specifically amplified in the V. salvator macromaculatus genome.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据