Article
Medicine, Research & Experimental
Vicky Caponigro, Anna L. Tornesello, Fabrizio Merciai, Danila La Gioia, Emanuela Salviati, Manuela G. Basilicata, Simona Musella, Francesco Izzo, Angelo S. Megna, Luigi Buonaguro, Eduardo Sommella, Franco M. Buonaguro, Maria L. Tornesello, Pietro Campiglia
Summary: This study utilized metabolomics and lipidomics to identify potential biomarkers for hepatocellular carcinoma (HCC). The identified biomarkers showed high diagnostic potential and could assist in the diagnosis of HCC, including in patients with negative AFP results. The predictive accuracy of metabolomics and lipidomics was superior to AFP in distinguishing HCC from chronic HCV conditions.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Yao Qian, Xiangyu Guo, Yunfang Wang, Zheng Ouyang, Xiaoxiao Ma
Summary: A mobility-modulated sequential dissociation (MMSD) strategy was developed for mass spectrometry imaging, enabling spatial lipidomics capable of multiplexed MS/MS imaging of 24 structurally distinct lipids. The method revealed a potential biomarker in a human hepatocellular carcinoma tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Xingyue Liu, Zhao Chen, Tao Wang, Xinrong Jiang, Xuetong Qu, Wei Duan, Fengna Xi, Zhengfu He, Jianmin Wu
Summary: The tissue imprinting technology using 2D nanoflakes-capped silicon nanowires allows for convenient and authentic revelation of molecular distribution on tissue samples, which is crucial for accurate cancer diagnosis.
Article
Oncology
Rong-Quan He, Jian-Di Li, Xiu-Fang Du, Yi-Wu Dang, Lin-Jie Yang, Zhi-Guang Huang, Li-Min Liu, Liu-Feng Liao, Hong Yang, Gang Chen
Summary: This study revealed that increased LPCAT1 expression correlated with poor prognosis in HCC patients and promoted HCC progression by enhancing cellular growth, migration, and metastasis. The cell cycle and chemical carcinogenesis were identified as the most enriched signaling pathways.
CANCER CELL INTERNATIONAL
(2021)
Article
Biochemical Research Methods
Haixing Wang, Ting Li, Qing Shen, Zhifang Liu, You-Wei Chen, Darong Huang, Jiancheng Yu, Long Wang
Summary: Liver cancer is the leading cause of cancer deaths worldwide, with hepatocellular carcinoma (HCC) accounting for over 90% of cases. This study optimized a mass spectrometry technique called rapid evaporative ionization mass spectrometry (REIMS) for analyzing HCC tumor tissue. Through the analysis of 12 human HCC tumor tissue specimens, it was found that certain free fatty acids and phospholipids were distributed differently in HCC, paracancerous tissue (PT), and noncancerous liver tissue (NCT). The upregulation of specific fatty acids and phospholipids was identified in HCC specimens, and a statistical model was established for real-time HCC tumor diagnosis with high sensitivity and specificity.
Article
Chemistry, Analytical
Tao Wang, Xingyue Liu, Xuetong Qu, Yuexin Li, Xiao Liang, Jianmin Wu
Summary: The study used nanostructure-assisted laser desorption/ionization mass spectrometry (NALDI-MS) to investigate the lipid response of HCC cells to anticancer drugs, revealing significant lipid profile changes after drug treatment and the strong correlation between fatty acids and cell viability. This method provides a potential candidate for precise tumor chemosensitivity assay.
Article
Biochemistry & Molecular Biology
Joshua M. Mitchell, Robert M. Flight, Hunter N. B. Moseley
Summary: This study investigated the differences in lipid profiles between cancerous and non-cancerous lung tissue samples from patients with NSCLC, identifying a shared metabolic phenotype across various genetically distinct NSCLC subtypes. The findings suggest that targeting the affected shared pathways may be beneficial for different NSCLC subtypes.
Article
Biochemical Research Methods
Olatomiwa O. Bifarin, Samyukta Sah, David A. Gaul, Samuel G. Moore, Ruihong Chen, Murugesan Palaniappan, Jaeyeon Kim, Martin M. Matzuk, Facundo M. Fernandez
Summary: Ovarian cancer is a deadly cancer that affects the female reproductive system and is often asymptomatic until later stages. Little is known about the metabolic changes in the early stages of high-grade serous ovarian cancer. This study used a mouse model and machine learning analysis to examine the temporal changes in serum lipidome. The results showed unique alterations in cell membrane stability, proliferation, and survival during cancer development and progression, providing potential targets for early detection and prognosis of ovarian cancer.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Oncology
Weidan Ji, Zhangxiao Peng, Bin Sun, Lei Chen, Qin Zhang, Minggao Guo, Changqing Su
Summary: The study confirmed that high expression of LpCat1 in HCC cells promoted cancer cell invasion, playing a crucial role in the progression and metastasis of HCC by interacting with STAT1.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Christoph Bookmeyer, Ulrich Roehling, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a rapidly growing method in the life sciences. The study introduces a novel post-ionization scheme based on single-photon induced chemical ionization using pulsed RF-Kr lamps, which effectively improves the ionization efficiency for analytes. Through the interplay with chemical dopants, the signal of various lipid molecules from mammalian tissue sections was significantly boosted.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Daisy Unsihuay, Ruichuan Yin, Daniela Mesa Sanchez, Manxi Yang, Yingju Li, Xiaofei Sun, Sudhansu K. Dey, Julia Laskin
Summary: The study introduces a novel experimental platform that combines nano-DESI and IM spectrometry for high spatial resolution imaging of molecules in mouse uterine tissues, providing unique molecular descriptors and revealing variations in isomeric composition through isomer-specific imaging.
ANALYTICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Tanja Bien, Krischan Koerfer, Jan Schwenzfeier, Klaus Dreisewerd, Jens Soltwisch
Summary: Molecular analysis on the single-cell level is rapidly growing in the life sciences. This study presents a sensitive approach to single-cell mass spectrometry based on high-resolution imaging and optical microscopy. The multimodal datasets allow for statistical analysis and direct connection of MS data with microscopy information, providing insights into intracellular lipid distributions and subcellular molecular profiles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Xiangyu Guo, Wenbo Cao, Xiaomin Fan, Zhiying Guo, Donghui Zhang, Haoyue Zhang, Xiaoxiao Ma, Jiahong Dong, Yunfang Wang, Wenpeng Zhang, Zheng Ouyang
Summary: Mass spectrometry imaging (MSI) of lipids in biological tissues can provide valuable information for biological research and disease diagnosis by correlating molecular distribution with pathological results. A new multiplexed MS2 imaging method (MSI-I-2) has been developed, allowing for MS2 images of 10 or more lipid species at each sampling spot, providing spatial structural lipidomic information. Coupled with on-tissue photochemical derivatization, imaging of 20 phospholipid C=C location isomers is also possible, resulting in enhanced molecular images with high structural definition for mouse brain and human liver cancer tissue sections. Spatially mapped t-distributed stochastic neighbor embedding has been utilized to visualize the tumor margin with the assistance of structural lipidomic information.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Huan Wu, Hui Cheng, Shengyong Luo, Can Peng, An Zhou, Zhiwu Chen, Hong Wu, Qinglin Li
Summary: The study demonstrates that HCS injection inhibits cell proliferation and induces apoptosis in HepG2 cells, as well as exhibits significant anti-HCC effects in an H22 hepatoma-bearing mouse model. Through UPLC-HRMS-based cellular metabolomics and lipidomics, 25 potential biomarkers associated with the intervention effect of HCS were identified, with UDP-GlcNAc and glutathione being confirmed. Metabolic pathway analysis revealed the involvement of glutathione, cysteine and methionine metabolism, sphingolipid metabolism, fatty acid biosynthesis, amino sugar, and nucleotide sugars in the anti-HCC effects of HCS injection.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Oncology
Shawn Lu Wen Tan, Erez Israeli, Russell E. Ericksen, Pierce K. H. Chow, Weiping Han
Summary: Alterations in metabolic pathways, especially in lipid metabolism, are significant in hepatocellular carcinoma. Understanding the lipidomic landscape of HCC is crucial for elucidating the mechanisms of tumor initiation and progression and finding potential therapeutic strategies.
SEMINARS IN CANCER BIOLOGY
(2022)